Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_bme_pgi 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_bme_pgi 1299499–1300697 1300697 1–1199 26 [0] [0] 26 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_bme_pgi = 12994980 (0.000)25 (0.850) 23/280 0.4 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_bme_pgi 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

GGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCA  >  NC_000913_3_bme_pgi/1300553‑1300842
                                                                                                                                                 |                                                                                                                                                
tcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataa                                                                                                                                               <  1:158909‑M2/4‑1 (MQ=255)
  aaattacagtttgttataagtatttccttacccgctgctcctccccatcatgttattttcgccacatcataatcctgggtctgctcaagaataattgaaatgatattattaattcctctgcctttggtagaggaaagtgctaaataata                                                                                                                                             <  2:75472‑M2/6‑1 (MQ=255)
         atttatgtataaagatgttcttaacttcttctccgtaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAtt                                                                                                                                      <  2:471376‑M2/13‑1 (MQ=255)
            tagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGtt                                                                                                                                   <  1:168815‑M2/16‑1 (MQ=255)
                   aaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattgcactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattat                                                                                                                            >  2:187586‑M2/127‑149 (MQ=255)
                             ttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTc                                                                                                                  >  2:36575‑M2/117‑149 (MQ=255)
                                         cctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACt                                                                                                      <  1:445133‑M2/45‑1 (MQ=255)
                                         cctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACt                                                                                                      <  1:451773‑M2/45‑1 (MQ=255)
                                           tcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTTTGCATTTCACTAATGGAacttt                                                                                                    >  2:508683‑M2/103‑147 (MQ=255)
                                              ccagcatgttatttgcgccacatcatgatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctgtggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAAt                                                                                                 <  1:292096‑M2/50‑1 (MQ=255)
                                                      ttattttcgccacatcataatcctgcgcgtgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCACAAAAg                                                                                         >  2:304267‑M2/92‑149 (MQ=255)
                                                       tattttcgccacatcataatcctgggcttgctgaagaatacttgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGa                                                                                        <  1:42028‑M2/59‑1 (MQ=255)
                                                         ttttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATa                                                                                      <  2:153763‑M2/61‑1 (MQ=255)
                                                            tcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactccctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGAc                                                                                   >  1:220470‑M2/86‑149 (MQ=255)
                                                              gccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTGAATGATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACAt                                                                                 >  2:381376‑M2/84‑149 (MQ=255)
                                                                   gtcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTa                                                                            >  1:40756‑M2/79‑149 (MQ=255)
                                                                   atcataatctttggcttgctgaataataatgaaaatgatattattaattccacttcctttggtagaggaaagtgcgaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATTGACATGCTTa                                                                            <  2:467875‑M2/71‑1 (MQ=255)
                                                                                             tgattcaagtggtattattaattccactgcatttcgtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGAttgaattgaa                                                  <  2:354280‑M2/97‑1 (MQ=255)
                                                                                                       gatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgt                                        >  1:534654‑M2/43‑149 (MQ=255)
                                                                                                             attaattccactgcctttggtagaggaaagtgataaATAATACTCGATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAGCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGc                                  >  1:426111‑M2/37‑149 (MQ=255)
                                                                                                                      actgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCa                         >  1:389466‑M2/28‑149 (MQ=255)
                                                                                                                        tgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAg                       >  2:195424‑M2/26‑149 (MQ=255)
                                                                                                                            tttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtat                   >  2:395008‑M2/22‑149 (MQ=255)
                                                                                                                              tggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTACGCGGCTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTGTAg                 >  1:507848‑M2/20‑149 (MQ=255)
                                                                                                                                           tgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGt    <  1:374374‑M2/143‑1 (MQ=255)
                                                                                                                                             ctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCa  >  2:530445‑M2/5‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCA  >  NC_000913_3_bme_pgi/1300553‑1300842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: