Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_bme_pgi 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_bme_pgi 1299499–1300697 1300697 1–1199 26 [0] [0] 26 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_bme_pgi = 12994980 (0.000)25 (0.850) 23/280 0.4 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_bme_pgi 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

TCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTC  >  NC_000913_3_bme_pgi/1299354‑1299643
                                                                                                                                                |                                                                                                                                                 
tcAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataa                                                                                                                                               <  1:158909‑M1/149‑5 (MQ=255)
  aaaTTACAGTTTGTTATAAGTATTTCCTTACCCGCTGCTCCTCCCCATCATGTTATTTTCGCCACATCATAATCCTGGGTCTGCTCAAGAATAATTGAAATGATATTATTAATTCCTCTGCCTTTGGTAGAGGAAAGTGCTAAataata                                                                                                                                             <  2:75472‑M1/149‑7 (MQ=255)
         atttatgtataaagatgtTCTTAACTTCTTCTCCGTACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                                                                      <  2:471376‑M1/142‑14 (MQ=255)
            tagttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtt                                                                                                                                   <  1:168815‑M1/149‑17 (MQ=255)
                   aaGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTGCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattat                                                                                                                            >  2:187586‑M1/1‑126 (MQ=255)
                             ttAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttc                                                                                                                  >  2:36575‑M1/1‑116 (MQ=255)
                                         cctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaact                                                                                                      <  1:445133‑M1/149‑46 (MQ=255)
                                         cctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaact                                                                                                      <  1:451773‑M1/149‑46 (MQ=255)
                                           tcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattttgcatttcactaatggaacttt                                                                                                    >  2:508683‑M1/1‑102 (MQ=255)
                                              ccAGCATGTTATTTGCGCCACATCATGATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTGTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaat                                                                                                 <  1:292096‑M1/149‑51 (MQ=255)
                                                      ttATTTTCGCCACATCATAATCCTGCGCGTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcacaaaag                                                                                         >  2:304267‑M1/1‑91 (MQ=255)
                                                       tATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATACTTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaaga                                                                                        <  1:42028‑M1/149‑60 (MQ=255)
                                                         ttttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagata                                                                                      <  2:153763‑M1/149‑62 (MQ=255)
                                                            tCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTCCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagac                                                                                   >  1:220470‑M1/1‑85 (MQ=255)
                                                              gCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttgaatgattgtgcatttcactactggaactgtaatcagaaaagatagacat                                                                                 >  2:381376‑M1/1‑83 (MQ=255)
                                                                   gtcataATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgctta                                                                            >  1:40756‑M1/2‑78 (MQ=255)
                                                                   atcatAATCTTTGGCTTGCTGAATAATAATGAAAATGATATTATTAATTCCACTTCCTTTGGTAGAGGAAAGTGCGAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagattgacatgctta                                                                            <  2:467875‑M1/149‑72 (MQ=255)
                                                                                             tgattcAAGTGGTATTATTAATTCCACTGCATTTCGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaa                                                  <  2:354280‑M1/147‑98 (MQ=255)
                                                                                                       gATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgt                                        >  1:534654‑M1/1‑42 (MQ=255)
                                                                                                             attaATTCCACTGCCTTTGGTAGAGGAAAGTGATAAataatactcgattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaagctctatttgattgaattgaaagatgtttgttaaggc                                  >  1:426111‑M1/1‑36 (MQ=255)
                                                                                                                      aCTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgca                         >  1:389466‑M1/1‑27 (MQ=255)
                                                                                                                        tGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaag                       >  2:195424‑M1/1‑25 (MQ=255)
                                                                                                                            tttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctat                   >  2:395008‑M1/1‑21 (MQ=255)
                                                                                                                              tGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcattacgcggctggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctgtag                 >  1:507848‑M1/1‑19 (MQ=255)
                                                                                                                                           tGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggt    <  1:374374‑M1/149‑144 (MQ=255)
                                                                                                                                             cTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggtca  >  2:530445‑M1/1‑4 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
TCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTC  >  NC_000913_3_bme_pgi/1299354‑1299643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: