mutation predictions | marginal predictions | summary statistics | genome diff | command line log
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breseq version 0.27.1 revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Predicted mutations | |||||
|---|---|---|---|---|---|
| evidence | position | mutation | annotation | gene | description |
| RA | 162,973 | G→A | C290Y (TGT→TAT) | hrpB → | putative ATP‑dependent helicase |
| RA | 236,541 | C→T | R159* (CGA→TGA) | dnaQ → | DNA polymerase III epsilon subunit |
| MC JC | 257,908 | Δ776 bp | [crl] | [crl] | |
| RA | 528,665 | C→T | pseudogene (26/491 nt) | ybbD → | pseudogene |
| RA | 700,805 | A→T | C264S (TGC→AGC) | nagC ← | N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor |
| RA | 720,946 | C→T | intergenic (+106/+110) | ybfK → / ← kdpE | uncharacterized protein/response regulator in two‑component regulatory system with KdpD |
| RA | 1,103,510 | G→A | intergenic (‑314/‑441) | csgD ← / → csgB | csgBAC operon transcriptional regulator/curlin nucleator protein, minor subunit in curli complex |
| RA | 1,143,125 | C→T | D415N (GAC→AAC) | rne ← | endoribonuclease; RNA‑binding protein;RNA degradosome binding protein |
| RA | 1,197,739 | A→G | S167P (TCC→CCC) | ymfE ← | e14 prophage; putative inner membrane protein |
| RA | 1,264,999 | T→A | intergenic (+29/‑13) | hemA → / → prfA | glutamyl tRNA reductase/peptide chain release factor RF‑1 |
| RA | 1,292,944 | T→C | intergenic (‑22/‑583) | hns ← / → tdk | global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase |
| RA | 1,401,844 | A→G | I471T (ATC→ACC) | abgB ← | p‑aminobenzoyl‑glutamate hydrolase, B subunit |
| RA | 1,420,038 | T→A | P68P (CCA→CCT) | racR ← | Rac prophage; putative DNA‑binding transcriptional regulator |
| RA | 1,434,459 | C→T | intergenic (‑235/+499) | ynaE ← / ← ttcC | cold shock protein, Rac prophage/pseudogene, prophage Rac integration site ttcA duplication;Phage or Prophage Related |
| RA | 1,458,804 | A→T | I181F (ATC→TTC) | paaG → | 1,2‑epoxyphenylacetyl‑CoA isomerase, oxepin‑CoA‑forming |
| RA | 1,530,076 | (A)7→8 | coding (155/483 nt) | ydcD → | putative immunity protein for RhsE |
| RA | 1,665,195 | (T)7→8 | intergenic (+75/‑120) | dmsD → / → clcB | twin‑argninine leader‑binding protein for DmsA and TorA/H(+)/Cl(‑) exchange transporter |
| RA | 1,681,932 | T→C | P33P (CCA→CCG) | ydgC ← | GlpM family inner membrane protein |
| RA | 1,896,268 | T→A | A33A (GCT→GCA) | nudL → | putative CoA pyrophosphohydrolase, weak 3‑phosphohydroxypyruvate phosphatase |
| MC JC | 1,978,503 | Δ776 bp | insB1–insA | insB1, insA | |
| RA | 1,983,761 | C→T | E437K (GAA→AAA) | araG ← | L‑arabinose ABC transporter ATPase |
| RA | 2,071,321 | A→G | intergenic (+110/‑218) | yeeP → / → flu | pseudogene, CP4‑44 prophage; 50S ribosome‑binding GTPase family;Phage or Prophage Related; putative histone/CP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter |
| RA | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
| RA | 2,191,432 | C→T | G72S (GGC→AGC) | yehC ← | putative periplasmic pilin chaperone |
| RA | 2,210,811 | C→T | pseudogene (1712/2001 nt) | yehQ → | pseudogene |
| RA | 2,234,512 | T→A | G160G (GGT→GGA) | preT → | dihydropyrimidine dehydrogenase, NADH‑dependent, subunit N |
| RA | 2,469,686 | C→T | Q186* (CAA→TAA) | gtrS → | serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage |
| RA | 2,563,518 | (A)5→6 | intergenic (+401/‑74) | yffP → / → yffQ | CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein |
| RA | 2,564,525 | G→A | M1M (ATG→ATA) † | yffS → | CPZ‑55 prophage; uncharacterized protein |
| RA | 2,614,327 | T→A | W132R (TGG→AGG) | focB → | putative formate transporter |
| RA | 2,644,342 | G→A | intergenic (‑59/+91) | rlmN ← / ← ndk | dual specificity 23S rRNA m(2)A2503, tRNA m(2)A37 methyltransferase, SAM‑dependent/multifunctional nucleoside diphosphate kinase and apyrimidinic endonuclease and 3'‑phosphodiesterase |
| RA | 2,752,089 | A→T | S99C (AGC→TGC) | recN → | recombination and repair protein |
| RA | 2,810,756 | G→A | intergenic (+77/‑14) | ygaH → / → mprA | putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux |
| RA | 2,887,352 | T→A | intergenic (‑133/+226) | ygcB ← / ← cysH | Cascade complex anti‑viral R‑loop helicase‑annealase Cas3/phosphoadenosine phosphosulfate reductase; PAPS reductase, thioredoxin dependent |
| RA | 2,996,015 | (T)8→9 | pseudogene (6/261 nt) | ygeN ← | pseudogene, orgB family, part of T3SS PAI ETT2 remnant |
| RA | 3,267,638 | T→C | intergenic (+40/‑216) | tdcR → / → yhaB | L‑threonine dehydratase operon activator protein/uncharacterized protein |
| RA | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| RA | 3,853,909 | A→G | intergenic (+267/‑13) | tisB → / → emrD | toxic membrane persister formation peptide, LexA‑regulated/multidrug efflux system protein |
| RA | 3,924,915 | A→T | V240E (GTG→GAG) | mnmG ← | 5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34 |
| RA | 3,950,467 | 2 bp→TT | intergenic (+47/‑39) | ilvL → / → ilvX | ilvG operon leader peptide/uncharacterized protein |
| RA | 3,971,690 | A→C | Q144P (CAG→CCG) | wecC → | UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase |
| RA | 4,235,689 | +A | intergenic (+282/‑217) | pgi → / → yjbE | glucosephosphate isomerase/extracellular polysaccharide production threonine‑rich protein |
| RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| RA | 4,310,950 | T→A | I11F (ATC→TTC) | alsA ← | D‑allose ABC transporter ATPase |
| RA | 4,572,279 | A→T | intergenic (+364/‑135) | yjiS → / → yjiT | DUF1127 family protein/pseudogene |
| RA | 4,615,859 | T→G | T403P (ACC→CCC) | yjjI ← | DUF3029 family protein, putative glycine radical enzyme |
| Unassigned missing coverage evidence | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | NC_000913 | 3423759–3424234 | 3424547–3424238 | 5–789 | 38 [36] | [35] 40 | [rrfD]–[rrlD] | [rrfD], [rrlD] |
| Unassigned new junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | 1207790 = | 10 (0.110) | 68 (0.810) | 46/264 | 0.3 | 90.7% | coding (290/630 nt) | stfP | e14 prophage; uncharacterized protein |
| ? | NC_000913 | 1209619 = | 5 (0.060) | pseudogene (1/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
| * | ? | NC_000913 | = 1207805 | 10 (0.110) | 60 (0.710) | 38/264 | 0.7 | 89.6% | coding (305/630 nt) | stfP | e14 prophage; uncharacterized protein |
| ? | NC_000913 | = 1209602 | 5 (0.060) | pseudogene (18/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
| * | ? | NC_000913 | = 1299498 | 0 (0.000) | 92 (1.000) | 50/288 | 0.3 | 100% | intergenic (+253/‑1684) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
| ? | NC_000913 | 1300698 = | 0 (0.000) | intergenic (+1453/‑484) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein | |||||