breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutation | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 2,399,955 | C→A | E56* (GAA→TAA) | lrhA ← | transcriptional repressor of flagellar, motility and chemotaxis genes |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | CP009273 | 80542 | 80613 | 72 | 2 [1] | [1] 2 | leuL/leuO | leu operon leader peptide/global transcription factor |
* | * | ÷ | CP009273 | 153676 | 153872 | 197 | 2 [1] | [0] 2 | [folK] | [folK] |
* | * | ÷ | CP009273 | 340589 | 340635 | 47 | 2 [1] | [0] 2 | yahL | uncharacterized protein |
* | * | ÷ | CP009273 | 559276 | 559343 | 68 | 3 [1] | [1] 2 | [sfmF]–[sfmZ] | [sfmF],[sfmZ] |
* | * | ÷ | CP009273 | 653424 | 653471 | 48 | 2 [1] | [1] 2 | flc/ybeM | fluoride efflux channel, dual topology membrane protein/pseudogene, C‑N hydrolase superfamily homology;putative enzyme; Not classified; putative amidase |
* | * | ÷ | CP009273 | 993170 | 993214 | 45 | 2 [0] | [1] 2 | ssuE/elfA | NAD(P)H‑dependent FMN reductase/laminin‑binding fimbrin subunit |
* | * | ÷ | CP009273 | 1072420 | 1072465 | 46 | 2 [0] | [1] 2 | putA | fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase |
* | * | ÷ | CP009273 | 1205059 | 1205074 | 16 | 15 [0] | [0] 14 | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related |
* | * | ÷ | CP009273 | 1350036 | 1350079 | 44 | 3 [1] | [0] 2 | sapA | antimicrobial peptide transport ABC transporter periplasmic binding protein |
* | * | ÷ | CP009273 | 1385065 | 1385112 | 48 | 2 [1] | [1] 2 | ymjC | pseudogene |
* | * | ÷ | CP009273 | 1459362 | 1459541 | 180 | 2 [1] | [1] 2 | ynbG | ynbG |
* | * | ÷ | CP009273 | 1647402 | 1647409 | 8 | 4 [0] | [0] 2 | rspB | putative Zn‑dependent NAD(P)‑binding oxidoreductase |
* | * | ÷ | CP009273 | 1725468 | 1725543 | 76 | 2 [1] | [0] 2 | lhr | putative ATP‑dependent helicase |
* | * | ÷ | CP009273 | 1726383 | 1726415 | 33 | 3 [1] | [0] 2 | lhr | putative ATP‑dependent helicase |
* | * | ÷ | CP009273 | 1783742 | 1783774 | 33 | 3 [0] | [1] 3 | aroH/ydiE | 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tryptophan repressible/hemin uptake protein HemP homolog |
* | * | ÷ | CP009273 | 1819132 | 1819163 | 32 | 3 [1] | [1] 2 | ves | cold‑ and stress‑inducible protein |
* | * | ÷ | CP009273 | 1842432 | 1842481 | 50 | 2 [1] | [0] 2 | ydjA | putative oxidoreductase |
* | * | ÷ | CP009273 | 1942448 | 1942460 | 13 | 2 [0] | [0] 3 | nudB | dihydroneopterin triphosphate pyrophosphatase |
* | * | ÷ | CP009273 | 1944734 | 1944784 | 51 | 2 [1] | [1] 2 | [aspS] | [aspS] |
* | * | ÷ | CP009273 | 2027296 | 2027415 | 120 | 2 [1] | [1] 2 | rseX/yedS | sRNA antisense regulator of ompA and ompC translation, Hfq‑dependent/pseudogene, outer membrane protein homology; putative outer membrane protein |
* | * | ÷ | CP009273 | 2051074 | 2051224 | 151 | 2 [0] | [1] 2 | yeeN/asnW | UPF0082 family protein/tRNA‑Asn |
* | * | ÷ | CP009273 | 2118378 | 2118434 | 57 | 3 [0] | [1] 4 | wcaI | putative glycosyl transferase |
* | * | ÷ | CP009273 | 2179065 | 2179089 | 25 | 2 [1] | [1] 3 | rcnR | transcriptional repressor of rcnA |
* | * | ÷ | CP009273 | 2201126 | 2201188 | 63 | 3 [1] | [1] 6 | yehM | uncharacterized protein |
* | * | ÷ | CP009273 | 2208322 | 2208788 | 467 | 2 [1] | [1] 2 | [mlrA] | [mlrA] |
* | * | ÷ | CP009273 | 2208850 | 2209085 | 236 | 2 [1] | [0] 2 | [mlrA] | [mlrA] |
* | * | ÷ | CP009273 | 2463688 | 2463744 | 57 | 2 [0] | [0] 2 | gtrS | serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage |
* | * | ÷ | CP009273 | 2568664 | 2568694 | 31 | 6 [0] | [1] 2 | eutP | putative P‑loop NTPase ethanolamine utilization protein |
* | * | ÷ | CP009273 | 2569405 | 2569429 | 25 | 2 [0] | [1] 2 | eutS/maeB | putative ethanol utilization carboxysome structural protein/malic enzyme: putative oxidoreductase/putative phosphotransacetylase |
* | * | ÷ | CP009273 | 2636976 | 2637008 | 33 | 3 [0] | [1] 2 | rlmN | dual specificity 23S rRNA m(2)A2503, tRNA m(2)A37 methyltransferase, SAM‑dependent |
* | * | ÷ | CP009273 | 2853082 | 2853089 | 8 | 2 [0] | [1] 3 | mutS/pphB | methyl‑directed mismatch repair protein/serine/threonine‑specific protein phosphatase 2 |
* | * | ÷ | CP009273 | 3354276 | 3354296 | 21 | 2 [0] | [0] 3 | gltD/gltF | glutamate synthase, 4Fe‑4S protein, small subunit/periplasmic protein |
* | * | ÷ | CP009273 | 3448775 | 3448865 | 91 | 3 [1] | [0] 10 | gspA/gspC | general secretory pathway component, cryptic/general secretory pathway component, cryptic |
* | * | ÷ | CP009273 | 3629073 | 3629086 | 14 | 3 [0] | [1] 2 | yhiM | acid resistance protein, inner membrane |
* | * | ÷ | CP009273 | 3745281 | 3745398 | 118 | 2 [0] | [1] 2 | [yiaU] | [yiaU] |
* | * | ÷ | CP009273 | 3750355 | 3750357 | 3 | 2 [0] | [1] 2 | yiaY | putative Fe‑containing alcohol dehydrogenase, Pfam00465 family |
* | * | ÷ | CP009273 | 3787017 | 3787111 | 95 | 2 [0] | [1] 2 | [yibB] | [yibB] |
* | * | ÷ | CP009273 | 3797648 | 3797810 | 163 | 3 [1] | [1] 2 | waaS | lipopolysaccharide core biosynthesis protein |
* | * | ÷ | CP009273 | 3805715 | 3805725 | 11 | 3 [0] | [0] 2 | yicR | UPF0758 family protein |
* | * | ÷ | CP009273 | 4036008 | 4036098 | 91 | 2 [1] | [1] 2 | srkA | stress response kinase A; MazF antagonist; Thr/Ser kinase involved in Cpx stress response |
* | * | ÷ | CP009273 | 4137111 | 4137123 | 13 | 2 [1] | [1] 3 | frwD | putative enzyme IIB component of PTS |
* | * | ÷ | CP009273 | 4294365 | 4294421 | 57 | 6 [0] | [1] 3 | ytcA/yjcS | putative inner membrane DUF1656 family protein; efflux pump associated/metallo‑beta‑lactamase superfamily protein |
* | * | ÷ | CP009273 | 4374142 | 4374154 | 13 | 2 [1] | [1] 2 | yjeM | putative transporter |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | CP009273 | 360104 = | 15 (0.800) | 13 (0.700) | 9/268 | 0.4 | 46.4% | intergenic (‑38/+557) | lacZ/lacI | pseudogene, truncated/DNA‑binding transcriptional repressor |
? | CP009273 | = 360286 | NA (NA) | intergenic (‑220/+375) | lacZ/lacI | pseudogene, truncated/DNA‑binding transcriptional repressor | |||||
* | ? | CP009273 | 1203246 = | 6 (0.320) | 14 (0.850) | 9/236 | 0.3 | 84.1% | coding (290/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | CP009273 | 1205075 = | 0 (0.000) | pseudogene (1/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | CP009273 | = 1203261 | 6 (0.320) | 14 (0.850) | 11/236 | 0.2 | 84.1% | coding (305/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | CP009273 | = 1205058 | 0 (0.000) | pseudogene (18/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | CP009273 | 3923686 = | 16 (0.860) | 5 (0.270) | 3/266 | 1.3 | 23.9% | coding (768/1497 nt) | ravA | hexameric AAA+ MoxR family ATPase, putative molecular chaperone |
? | CP009273 | 4033280 = | NA (NA) | noncoding (2402/2905 nt) | rrlA | 23S ribosomal RNA of rrnA operon | |||||
* | ? | CP009273 | 4363113 = | 15 (0.860) | 12 (0.690) | 3/248 | 1.2 | 44.6% | intergenic (+134/+69) | yjeI/yjeJ | DUF4156 family lipoprotein/uncharacterized protein |
? | CP009273 | 4364315 = | NA (NA) | noncoding (1/99 nt) | RIP329 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site | RIP329 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site |