mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | TF_KALE_lrp_A20_F16_I1_R1_S39_L001_R1_001.good.fq | 365,988 | 47,156,259 | 100.0% | 128.8 bases | 137 bases | 97.6% |
| errors | TF_KALE_lrp_A20_F16_I1_R1_S39_L001_R2_001.good.fq | 365,988 | 47,157,886 | 100.0% | 128.9 bases | 137 bases | 95.8% |
| errors | TF_KALE_lrp_A20_F16_I1_R1_S39_L002_R1_001.good.fq | 364,047 | 46,890,884 | 100.0% | 128.8 bases | 137 bases | 98.3% |
| errors | TF_KALE_lrp_A20_F16_I1_R1_S39_L002_R2_001.good.fq | 364,047 | 46,892,953 | 100.0% | 128.8 bases | 137 bases | 97.0% |
| errors | TF_KALE_lrp_A20_F16_I1_R1_S39_L003_R1_001.good.fq | 364,136 | 46,821,326 | 100.0% | 128.6 bases | 137 bases | 98.2% |
| errors | TF_KALE_lrp_A20_F16_I1_R1_S39_L003_R2_001.good.fq | 364,136 | 46,822,066 | 100.0% | 128.6 bases | 137 bases | 97.2% |
| errors | TF_KALE_lrp_A20_F16_I1_R1_S39_L004_R1_001.good.fq | 372,961 | 47,962,554 | 100.0% | 128.6 bases | 137 bases | 98.5% |
| errors | TF_KALE_lrp_A20_F16_I1_R1_S39_L004_R2_001.good.fq | 372,961 | 47,963,161 | 100.0% | 128.6 bases | 137 bases | 97.7% |
| total | 2,934,264 | 377,667,089 | 100.0% | 128.7 bases | 137 bases | 97.5% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 78.8 | 4.1 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 1831 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 125 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.008 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.82774 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction | 0.1 |
| Junction allow suboptimal matches | FALSE |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.2.6 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 21:24:11 22 Apr 2021 | 21:25:01 22 Apr 2021 | 50 seconds |
| Read alignment to reference genome | 21:25:01 22 Apr 2021 | 21:31:09 22 Apr 2021 | 6 minutes 8 seconds |
| Preprocessing alignments for candidate junction identification | 21:31:09 22 Apr 2021 | 21:31:45 22 Apr 2021 | 36 seconds |
| Preliminary analysis of coverage distribution | 21:31:45 22 Apr 2021 | 21:33:26 22 Apr 2021 | 1 minute 41 seconds |
| Identifying junction candidates | 21:33:26 22 Apr 2021 | 21:33:26 22 Apr 2021 | 0 seconds |
| Re-alignment to junction candidates | 21:33:26 22 Apr 2021 | 21:34:56 22 Apr 2021 | 1 minute 30 seconds |
| Resolving best read alignments | 21:34:56 22 Apr 2021 | 21:35:46 22 Apr 2021 | 50 seconds |
| Creating BAM files | 21:35:46 22 Apr 2021 | 21:37:17 22 Apr 2021 | 1 minute 31 seconds |
| Tabulating error counts | 21:37:17 22 Apr 2021 | 21:37:49 22 Apr 2021 | 32 seconds |
| Re-calibrating base error rates | 21:37:49 22 Apr 2021 | 21:37:52 22 Apr 2021 | 3 seconds |
| Examining read alignment evidence | 21:37:52 22 Apr 2021 | 21:44:47 22 Apr 2021 | 6 minutes 55 seconds |
| Polymorphism statistics | 21:44:47 22 Apr 2021 | 21:44:48 22 Apr 2021 | 1 second |
| Output | 21:44:48 22 Apr 2021 | 21:44:57 22 Apr 2021 | 9 seconds |
| Total | 20 minutes 46 seconds | ||
