breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
MC JC | 257,908 | Δ776 bp | [crl] | [crl] | |
RA | 1,529,185 | C→T | pseudogene (1284/2037 nt) | rhsE → | pseudogene, Rhs family |
RA | 1,529,203 | C→A | pseudogene (1302/2037 nt) | rhsE → | pseudogene, Rhs family |
RA | 1,529,218 | C→T | pseudogene (1317/2037 nt) | rhsE → | pseudogene, Rhs family |
RA | 1,756,497 | G→T | C267F (TGT→TTT) | pykF → | pyruvate kinase I |
RA | 1,927,949 | A→C | R297R (CGT→CGG) | ptrB ← | protease II |
MC JC | 1,978,503 | Δ776 bp | insB1–insA | insB1, insA | |
RA | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
RA | 3,316,893 | C→T | E212K (GAA→AAA) | nusA ← | transcription termination/antitermination L factor |
RA | 3,440,086 | C→A | G315V (GGC→GTC) | rpoA ← | RNA polymerase, alpha subunit |
RA | 3,546,391 | C→T | intergenic (+192/‑168) | nfuA → / → gntT | Fe/S biogenesis protein, putative scaffold/chaperone protein/gluconate transporter, high‑affinity GNT I system |
RA | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
RA | 4,158,779 | T→G | L97W (TTG→TGG) | oxyR → | oxidative and nitrosative stress transcriptional regulator |
RA | 4,235,408 | Δ1 bp | intergenic (‑2175/‑499) | lysC ← / → yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
RA | 4,296,382 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913_3_bme_pgi | 3423729–3424234 | 3424577–3424238 | 5–849 | 38 [37] | [36] 38 | [rrfD]–[rrlD] | [rrfD],[rrlD] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913_3_bme_pgi | = 1299498 | 0 (0.000) | 74 (0.860) | 58/248 | 0.6 | 100% | intergenic (+253/‑1684) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
? | NC_000913_3_bme_pgi | 1300698 = | 0 (0.000) | intergenic (+1453/‑484) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein | |||||
* | ? | NC_000913_3_bme_pgi | = 1979270 | NA (NA) | 1348 (15.200) | 264/256 | -0.0 | NA | intergenic (‑55/‑483) | insA/uspC | IS1 repressor TnpA/universal stress protein |
? | NC_000913_3_bme_pgi | = 4235548 | NA (NA) | intergenic (‑2315/‑359) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein | |||||
* | ? | NC_000913_3_bme_pgi | 3583427 = | NA (NA) | 32 (0.620) +53 bp |
20/150 | 2.4 | NA | intergenic (+9/‑56) | yrhA/insA | pseudogene, interrupted by IS1E/IS1 repressor TnpA |
? | NC_000913_3_bme_pgi | 4235463 = | NA (NA) | noncoding (42/77 nt) | REP313 (repetitive extragenic palindromic) element; contains 2 REP sequences | REP313 (repetitive extragenic palindromic) element; contains 2 REP sequences | |||||
* | ? | NC_000913_3_bme_pgi | = 3584195 | NA (NA) | 1161 (13.100) | 255/256 | -0.0 | 93.5% | intergenic (+15/‑1) | insB1/yrhA | IS1 transposase B/pseudogene, interrupted by IS1E |
? | NC_000913_3_bme_pgi | 4221371 = | 80 (0.900) | coding (1117/2187 nt) | arpA | ankyrin repeat protein |