Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_bme_pgi	1,927,949	A→C	R297R (CGT→CGG)	ptrB ←	protease II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_bme_pgi	1,927,949	0	A	C	100.0%	258.3 / NA	74	R297R (CGT→CGG)	ptrB	protease II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (35/39);  total (35/39)

CGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAAAACAAACGGCTCGGCATCGGCCATTTCCGCGTCCAGCAGG  >  NC_000913_3_bme_pgi/1927806‑1928090                                                                                                                                                |                                                                                                                                              cGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGtat                                                                                                                                         <  1:445040/150‑1 (MQ=255)  ggTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGtata                                                                                                                                        >  4:164918/1‑150 (MQ=255)  ggTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGtata                                                                                                                                        >  1:73904/1‑150 (MQ=255)   gTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATaa                                                                                                                                       >  4:172502/1‑150 (MQ=255)   gTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATaa                                                                                                                                       <  2:508926/150‑1 (MQ=255)   gTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGCGTCCGGTATaa                                                                                                                                       >  4:67413/1‑150 (MQ=255)        tttGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCaa                                                                                                                                  >  3:91875/1‑150 (MQ=255)              gcAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGtttt                                                                                                                            <  4:91875/150‑1 (MQ=255)                aaaCTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTg                                                                                                                          <  4:150768/150‑1 (MQ=255)                 aaCTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCATTCCAGCATGATGTTTGCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGc                                                                                                                         >  3:149920/1‑150 (MQ=255)                      gTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTgg                                                                                                                    >  3:381623/1‑150 (MQ=255)                             cgcgCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTgg                                                                                                             <  3:560951/150‑1 (MQ=255)                                gcTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAAc                                                                                                          >  3:22062/1‑150 (MQ=255)                                  tGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGCGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGc                                                                                                        <  3:67413/150‑1 (MQ=255)                                      gCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGAt                                                                                                    >  4:501738/1‑150 (MQ=255)                                      gCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGAt                                                                                                    <  4:636725/150‑1 (MQ=255)                                            cAACCACCAGCCAGTCGGTAAACAGCGGAAATCTTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAAc                                                                                              >  2:490361/1‑150 (MQ=255)                                             aaCCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTg                                                                                                              <  1:540652/133‑1 (MQ=255)                                             aaCCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTg                                                                                                              >  2:540652/1‑133 (MQ=255)                                             aaCCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACg                                                                                             >  2:516987/1‑150 (MQ=255)                                                caccaGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATg                                                                                          >  1:316007/1‑150 (MQ=255)                                                caccaGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATg                                                                                          <  3:632802/150‑1 (MQ=255)                                                   cagccagTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTg                                                                                       <  3:501738/150‑1 (MQ=255)                                                   cagccagTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTg                                                                                       <  2:426741/150‑1 (MQ=255)                                                    agccagTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTgg                                                                                      <  1:1062592/150‑1 (MQ=255)                                                     gccagTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGt                                                                                     <  1:516987/150‑1 (MQ=255)                                                      ccagTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTa                                                                                    >  2:241693/1‑150 (MQ=255)                                                        agTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGt                                                                                  >  1:945687/1‑150 (MQ=255)                                                             gTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCa                                                                             >  2:864571/1‑150 (MQ=255)                                                                aaCAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCAAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGCTGCTGGTAGTGATCAAgg                                                                          <  4:249169/150‑1 (MQ=255)                                                                      gTAAACCCTTCCAGCATGATGTTTTCGGGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTAt                                                                    >  1:769968/1‑150 (MQ=255)                                                                      gTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGtat                                                                                                                                         >  1:240431/1‑81 (MQ=255)                                                                      gTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGtat                                                                                                                                         <  2:240431/81‑1 (MQ=255)                                                                        aaaCCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTc                                                                  <  3:635479/150‑1 (MQ=255)                                                                        aaaCCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCACATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTc                                                                  <  1:315690/150‑1 (MQ=255)                                                                          aCCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGt                                                                <  1:228428/150‑1 (MQ=255)                                                                          aCCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTAGAAAGAAAATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGt                                                                <  4:260141/150‑1 (MQ=255)                                                                           cccTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTg                                                               <  2:858744/150‑1 (MQ=255)                                                                            ccTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGa                                                              <  2:316007/150‑1 (MQ=255)                                                                                 cAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCaa                                                                            <  2:734117/131‑1 (MQ=255)                                                                                 cAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCaa                                                                            >  1:734117/1‑131 (MQ=255)                                                                                  aGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTg                                                        <  4:381623/150‑1 (MQ=255)                                                                                    cATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAGACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTgcg                                                      <  2:73904/150‑1 (MQ=255)                                                                                      tgatgTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCa                                                                                                       <  2:84654/99‑1 (MQ=255)                                                                                      tgatgTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCa                                                                                                       >  1:84654/1‑99 (MQ=255)                                                                                          gTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTgcggcgcgg                                                <  1:490361/150‑1 (MQ=255)                                                                                          gTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTgcggcgcgg                                                <  3:172502/150‑1 (MQ=255)                                                                                           ttttCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTg                                                        >  1:1011565/1‑141 (MQ=255)                                                                                           ttttCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTg                                                        <  2:1011565/141‑1 (MQ=255)                                                                                              tCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAAAAAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGa                                            <  4:22062/150‑1 (MQ=255)                                                                                                  gTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGaaaaa                                        <  3:164918/150‑1 (MQ=255)                                                                                                   tGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAaaac                                       <  1:787284/150‑1 (MQ=255)                                                                                                       ggAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAaaacaaac                                   >  1:164594/1‑150 (MQ=255)                                                                                                        gAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAgg                                                                          <  3:294865/110‑1 (MQ=255)                                                                                                        gAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAgg                                                                          >  4:294865/1‑110 (MQ=255)                                                                                                             aaCTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAAAACAAACGGCTCg                             <  3:325388/150‑1 (MQ=255)                                                                                                             aaCTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTAGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAAAACAAACGGCTCg                             >  3:543340/1‑150 (MQ=255)                                                                                                               ctctTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAAAACAAACGGCTCGGc                           >  1:778250/1‑150 (MQ=255)                                                                                                                  ttCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAAc                                                                                                          <  4:465235/68‑1 (MQ=255)                                                                                                                  ttCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAAc                                                                                                          >  3:465235/1‑68 (MQ=255)                                                                                                                  ttCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAAAACAAACGGCTCGGCATc                        >  1:797152/1‑150 (MQ=255)                                                                                                                    cccACTGTTGCTCATCACTCATACGGGTCCGGTATAAGCCAAAGTTTTTGCTGTTGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCCAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAAAACAAACGGCTCGGCATCgg                      >  2:629179/1‑150 (MQ=255)                                                                                                                         tGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGaa                                                                                                           >  2:826605/1‑60 (MQ=255)                                                                                                                         tGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGaa                                                                                                           <  1:826605/60‑1 (MQ=255)                                                                                                                             gCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAAAACAAACGGCTCGGCATCGGCCATTTCcg             >  2:227019/1‑150 (MQ=255)                                                                                                                              cTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAAAACAAACGGCTCGGCATCGGCCATTTCcgc            <  3:260312/150‑1 (MQ=255)                                                                                                                                 atcaCGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGGTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAATGCTGTATTCGTGATCTTTGCGGCGCGGACGAAGAACACAGCGCTCGTCATCGGGCATTTCCGCGTc         >  3:356669/1‑150 (MQ=255)                                                                                                                                  tcaCGCATAAGGGTCCGGTATAAGCTAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACAATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAAAACAAACGGCTCGGCATCGGCAATTTCCGCGTcc        >  2:796317/1‑150 (MQ=255)                                                                                                                                    aCGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAg                                                                           <  2:1043389/81‑1 (MQ=255)                                                                                                                                    aCGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAg                                                                           >  1:1043389/1‑81 (MQ=255)                                                                                                                                     cGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAAAACAAACGGCTCGGCATCGGCCATTTCCGCGTCcagc     <  2:164594/150‑1 (MQ=255)                                                                                                                                      gCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAAAACAAACGGCTCGGCATCGGCCATTTCCGCGTCcagca    >  3:334359/1‑150 (MQ=255)                                                                                                                                      gCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAAAACAAACGGCTCGGCATCGGCCATTTCCGCGTCcagca    <  2:945687/150‑1 (MQ=255)                                                                                                                                        aTACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAAAACAAACGGCTCGGCATCGGCCATTTCCGCGTCCAGCAgg  >  4:402705/1‑150 (MQ=255)                                                                                                                                                |                                                                                                                                              CGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAAAACAAACGGCTCGGCATCGGCCATTTCCGCGTCCAGCAGG  >  NC_000913_3_bme_pgi/1927806‑1928090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑