Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,755,755	IS5 (+) +4 bp	coding (58‑61/1413 nt)	pykF →	pyruvate kinase I

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1397238	NA (NA)	43 (1.060)	34/284	0.2	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	1755755 =	0 (0.000)					coding (58/1413 nt)	pykF	pyruvate kinase I
*	?	NC_000913	= 1755758	0 (0.000)	27 (0.670)	23/284	0.9	100%	coding (61/1413 nt)	pykF	pyruvate kinase I
	?	NC_000913	= 2102943	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

ATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGATTACATCATGATTATGGCGA  >  NC_000913/1397102‑1397377                                                                                                                                         |                                                                                                                                            aTAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCctaact                                                                                                                                       >  1:473587/1‑143 (MQ=11)   aaCGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCctaactaa                                                                                                                                     <  1:22526/143‑1 (MQ=32)   aaCGCCTTAAAGGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCACAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCctaactaa                                                                                                                                     <  1:22353/143‑1 (MQ=17)       ccTTAAATGGCGAAGAAACGGTCTAAATAGGCTGCTTCCAGGCATTTACGGGAGAAAAAATCGGCTCAAAACTGAAGGAATGAAATGACTGAGTCAGCCGAGACGAATTTCCCGGCTTATTCGCACGTTCCCTAACTAATGaa                                                                                                                                 >  2:223507/1‑143 (MQ=11)          tAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGc                                                                                                                              >  2:81817/1‑143 (MQ=37)             aTGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGtt                                                                                                                           >  1:558169/1‑143 (MQ=40)                 cGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCTAGAAGAATTTACCCGATTATACGCAACCTCCCTCACTAATGAATCCGTTGAtt                                                                                                                       >  2:348597/1‑143 (MQ=11)                    agaaACGGTCTAAATAGGCTGATTCCAGGCATTTACGGGAGAAAAAATCGGCTCAAACGTGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTCACTAATCAATGCGTTGATTGtc                                                                                                                    >  1:645564/1‑142 (MQ=255)                                  tAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTAAGCCGAGAAGAATTTCCCAGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTAATTGTAAATCCAGCTAagag                                                                                                      >  2:244332/1‑143 (MQ=255)                                          ttCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTGACTAATCAATGCGt                                                                                                                            >  1:124101/1‑113 (MQ=255)                                          ttCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTGACTAATCAATGCGt                                                                                                                            <  2:124101/113‑1 (MQ=255)                                          ttCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGtt                                                                                              <  1:81817/143‑1 (MQ=255)                                            cAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGtttt                                                                                            <  1:16453/143‑1 (MQ=255)                                                gCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCaga                                                                                        >  1:595185/1‑143 (MQ=255)                                                    ttACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGACAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAACAg                                                                                    >  2:400614/1‑143 (MQ=255)                                                        gggAGAAAAAATCGGCTCAAAAAAGCCGAAATGATAGTATTGTGGCAGCCGAAAAGAATATCACCACTTATTCGTACCTTCCCTAACTAAACAATTAAATGATTATAAATACACCTAAGACGTGAGCATGTCAGAGCAAACaa                                                                                >  2:54466/1‑143 (MQ=9)                                                            aaaaaaaTCAGCTGAAGCATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTATTCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGt                                                                            <  1:423107/142‑1 (MQ=255)                                                             aaaaaaTCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAACTCCAGCTAAGAGGTGAGGTCTTCAGAGCAGACAACgcag                                                                           >  2:664933/1‑140 (MQ=255)                                                              aaaaaTCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGGGa                                                                          >  1:76679/1‑143 (MQ=255)                                                                aaaTCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGCTTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGGCAACGGTGaaa                                                                        >  2:90273/1‑143 (MQ=255)                                                                 aaTCGGCTCAAATATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCTCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAAt                                                                       <  2:374644/143‑1 (MQ=255)                                                                   tCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTCTAAATCCAGCTAAGAGGCGAGGCTTTCCGAGCAGACAACGGTGAAATGt                                                                     >  2:508043/1‑143 (MQ=255)                                                                        tCAAACAGCAAGAACTGAACGGCCTGAGTCAGCCGAGAAGAAGTTCCCCGCTTATTCGCACCTTCCCTAACGAATCAATGCGTTGATTGTACATCCAGCTAAGAGGTGAGCTTTTCAGAGCAGACAACGGTGAAATGTCATgg                                                                <  2:384980/143‑1 (MQ=25)                                                                         cAAACATGAAGAAAGGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGt                                                               <  2:689669/143‑1 (MQ=255)                                                                             cATGAAGAACTGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAGTCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTg                                                           <  2:240812/143‑1 (MQ=255)                                                                              aTGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTGTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGt                                                          >  2:448113/1‑143 (MQ=255)                                                                                 aagaaATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTAc                                                       >  1:91409/1‑143 (MQ=255)                                                                                 aagaaATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTAc                                                       >  2:667780/1‑143 (MQ=255)                                                                                    atgtcaaatgaCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGtt                                                    <  2:625852/138‑1 (MQ=255)                                                                                      atgaaatgaCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTa                                                  >  2:56693/1‑143 (MQ=255)                                                                                       tgaaatgaCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAg                                                 <  1:711343/143‑1 (MQ=255)                                                                                       ggaaatgaCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAg                                                 <  2:443620/142‑1 (MQ=255)                                                                                        gaaatgaCTGAGGCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGCTTGCAAATCGAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAgg                                                <  1:656139/143‑1 (MQ=255)                                                                                          aatgaCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTa                                              >  1:82268/1‑143 (MQ=255)                                                                                           atgaCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTaa                                             <  1:696253/143‑1 (MQ=255)                                                                                                   gTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAAt                                     <  2:595185/143‑1 (MQ=255)                                                                                                   gTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGGTTAGGTAACAGGAAAt                                     >  2:639230/1‑143 (MQ=255)                                                                                                      aGCCGAGAAGAATTTGTCGGCTTATTCGGAACTTCCCTAACTAAGCAGTGAGTTGATGGGAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTg                                  <  1:184011/143‑1 (MQ=255)                                                                                                       gCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGTCAACGGTGAAATGTCATGGTATTGTTACGTTGAGGTAACAAGAAATTTga                                 >  2:439416/1‑142 (MQ=255)                                                                                                       gCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGt                                 <  1:405969/143‑1 (MQ=255)                                                                                                        ccGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGGAATGTCATGGTGCTGTGAAGGCTAGGTAACAAGAAATTTGTc                                >  2:624297/1‑143 (MQ=38)                                                                                                        ccGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTc                                <  2:558169/143‑1 (MQ=255)                                                                                                            gaagaaTTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGca                            >  1:35467/1‑143 (MQ=255)                                                                                                                   ttCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTATGAGGGGAGATTCAAAGAGCAGACAACGGTGTAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCAAAAGGAt                     >  1:420294/1‑143 (MQ=255)                                                                                                                   ttCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATATCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGAt                     <  1:179628/143‑1 (MQ=255)                                                                                                                   ttCCCCGCTTAGTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGTTTTTTAGTTCGGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGAt                     <  1:201069/143‑1 (MQ=255)                                                                                                                      cccGCTTATTCGCACCTTCCCTGACTAATCGATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGATTAc                  <  1:627190/143‑1 (MQ=255)                                                                                                                          cTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGATTAcatca              <  1:667780/143‑1 (MQ=255)                                                                                                                           ttATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGATTAcatcat             >  1:485527/1‑143 (MQ=255)                                                                                                                            tATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTCTAGGTAACAAGAAATTTGTCTGCACAAGGATTACATCATg            <  1:54114/143‑1 (MQ=255)                                                                                                                             agtCGCGCCTGCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGATTACATCATGa           <  2:133294/141‑1 (MQ=255)                                                                                                                               tCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTGTCAGAGCAGACAACGGTGAAAGGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGATTACATCATGAtt         >  2:593062/1‑143 (MQ=255)                                                                                                                               ctgCACCTTCCCTAACTAATCGATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGATTACATCATGAtt         <  2:76679/141‑1 (MQ=255)                                                                                                                                 gCACCTTCCCTAACTATTTATTTCGTTTATTGTATATCCAGTTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGATTACATCATGATTAt       <  1:181941/143‑1 (MQ=255)                                                                                                                                   aCCTTTCGTATCTTATAAAGGCGTGGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGATTACATCATGATTATgg     <  2:358715/143‑1 (MQ=255)                                                                                                                                      ttCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGATTACATCATGATTATGGCGa  >  1:364677/1‑143 (MQ=255)                                                                                                                                         |                                                                                                                                            ATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGATTACATCATGATTATGGCGA  >  NC_000913/1397102‑1397377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.