Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 1,755,755 IS5 (+) +4 bp coding (58‑61/1413 nt) pykF → pyruvate kinase I

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 1397238NA (NA)43 (1.060) 34/284 0.2 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 1755755 = 0 (0.000)coding (58/1413 nt) pykF pyruvate kinase I
* ? NC_000913 = 17557580 (0.000)27 (0.670) 23/284 0.9 100% coding (61/1413 nt) pykF pyruvate kinase I
?NC_000913 = 2102943 NA (NA)noncoding (1/1195 nt) IS5 repeat region

TACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1755621‑1755758
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTC  <  NC_000913/2102943‑2102804
                                                                                                                                                                                                                                                                                      
TACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACAAAGACTGTCATGAAAAAGACCAAAATTGTGTGCACCAGCGGACCGAAGAGCGAATCGGCAGAGAGGTGAGGGAAG                                                                                                                                         >  2:274337/1‑143
       TTCGCTTGCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGGGAAGGTGCGAA                                                                                                                                  <  1:560731/143‑1
         CGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGGGAAGGTGCGAACA                                                                                                                                <  2:229213/143‑1
                               AAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGT                                                                                                          <  2:128111/143‑1
                                               ATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCGGAAGAGATGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCA                                                                                          >  2:712396/1‑143
                                                        AACTTAAAGACTAAGCCTGTCATGACAAGGATAATAATTAGCTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGG                                                                                 >  2:717939/1‑143
                                                        AACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGG                                                                                 >  1:487932/1‑143
                                                         ACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGG                                                                                <  2:388682/143‑1
                                                         ACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGGGAAGGCGCGCACAAGTCCCTGATATGAGATCATGCTTGTCATCGGGAGCCATAGAACAGGG                                                                                >  2:652323/1‑143
                                                                 ACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGACAACCGAATCTGAAGAGATGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGCACAGGGTTCATCgt                                                                        >  1:318297/1‑141
                                                                       ACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCA                                                                  <  1:528056/143‑1
                                                                                  AAAGACCAAAAGTGTTTGCACCATCGGACCGAAAACCGAATCGGAAGAGATGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGTGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCT                                                       <  1:311338/143‑1
                                                                                                TTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAA                                         >  1:687789/1‑143
                                                                                                  TGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGGGAAGGTGTGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATT                                       >  1:31996/1‑143
                                                                                                       CATCGGACCGATAACCGAATCTGATGTGATGTTCGGGGAGGTGGCAACAGGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCA                                  <  1:608357/143‑1
                                                                                                       CATCGGACCGAAAACCGAATCTGAAGAGATGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCA                                  <  1:712396/143‑1
                                                                                                        ATCGGACCGAAAACCGAATCTGAAGAGATGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAG                                 >  1:621103/1‑143
                                                                                                           GGACCGAAATCCGAATATGAGGAGATGTTAGGGAGGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCACCGACAGTGAATTCAGCAGTAA                              <  2:214662/143‑1
                                                                                                            GACCGAAAACCGAATCTGAAGAGATGTTAGGGAACGTGCGAACAAGTCCCTGATATAAGATCATATTAGTCATATGGAGCCATAGAACAGGGATCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAG                             >  1:44021/1‑143
                                                                                                               CGAAAACCGAATCTGAAGAGATGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGCAAGCGC                          >  2:338460/1‑143
                                                                                                               CGAAAACCGAATCTGAAGAGATGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTCAATTCAGCAGTCAGCGC                          >  1:585678/1‑143
                                                                                                               CGAAAACCGAATCTGAAGAGATGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACATTCGCCGACAGTGAATTCAGCAGTACGCcc                          >  2:242084/1‑141
                                                                                                                             GAAGAGATGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAACTCAGCAGTAAGCGCCGTCAGACCAGCAA            >  2:255826/1‑143
                                                                                                                             GAAGAGATGTGAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAA            <  1:353898/143‑1
                                                                                                                              AAGAGATGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAGAA           >  2:59134/1‑143
                                                                                                                                      TTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGAGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATcat   >  2:566162/1‑140
                                                                                                                                       TAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAAGTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGGTTTTC  >  1:9132/1‑143
                                                                                                                                                                                                                                                                                      
TACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1755621‑1755758
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTC  <  NC_000913/2102943‑2102804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.