Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 2,375,828 IS186 (+) +4 bp coding (132‑135/963 nt) menC ← O‑succinylbenzoyl‑CoA synthase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 2375828 =0 (0.000)20 (0.720) 18/276 0.6 100% coding (135/963 nt) menC O‑succinylbenzoyl‑CoA synthase
?NC_000913 = 2515617 NA (NA)noncoding (1345/1345 nt) IS186 repeat region
* ? NC_000913 = 23758310 (0.000)27 (0.970) 21/276 0.3 100% coding (132/963 nt) menC O‑succinylbenzoyl‑CoA synthase
?NC_000913 2514273 = NA (NA)noncoding (1/1345 nt) IS186 repeat region

AAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGT  >  NC_000913/2514131‑2514408
                                                                                                                                              |                                                                                                                                       
aaaGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAtt                                                                                                                                         <  1:249894/143‑1 (MQ=255)
aaaGGCCGGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAtt                                                                                                                                         <  1:112492/143‑1 (MQ=255)
 aaGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTCCGGCGCTAACCTGGTGAGTGTGCTGTCTGGGTCAATCGCAGCACTGGTGCTGTAAGGCCGTACATAAAAAAGCCGGGGTTAATTc                                                                                                                                        >  1:38039/1‑143 (MQ=255)
           tgaaGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTGCCATCAgcgc                                                                                                                              >  2:364937/1‑143 (MQ=255)
           tgaaGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATCCCCATCAgcgc                                                                                                                              >  2:76977/1‑143 (MQ=255)
             aaGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTa                                                                                                                            >  1:142897/1‑143 (MQ=255)
              agagCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTaa                                                                                                                           <  1:261088/143‑1 (MQ=255)
                           gTGGTTTTTCGCTTGTGTCTGAGGCTGGTTTACGGCTCTACCCTGGGGAGTGTGCTGTCAGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTtgtg                                                                                                              <  2:256619/143‑1 (MQ=255)
                               tttCGCGCTTCGGTGTGAGGCTGGTTTACGGCTCTACCCTGGTGGGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTAt                                                                                                          <  2:477937/143‑1 (MQ=255)
                                ttCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTAtt                                                                                                         <  1:176683/143‑1 (MQ=255)
                                ttCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGAAAATTCCCATAACCGCTAACTTAAGAGCTGTGGTAtt                                                                                                         >  2:147271/1‑143 (MQ=255)
                                            tCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATCAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGAGTTGAAGTATTACGCCTGACATg                                                                                             >  2:485585/1‑143 (MQ=39)
                                                aaGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGCTGTGGTATTAGGCCTGATATGAgtt                                                                                         >  2:126041/1‑143 (MQ=255)
                                                                     ctgctgAGTGTGCGGTCTGCGCCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTActct                                                                    <  2:435202/143‑1 (MQ=255)
                                                                       ggTGAGTGTGCTGTCTGCGTCAATCGCAGAACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCa                                                                  <  1:144784/143‑1 (MQ=255)
                                                                            gtgtGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATa                                                             >  1:263510/1‑143 (MQ=255)
                                                                                     cagggTCAAGCGCAGCAGTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGGTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAg                                                    <  2:383374/139‑1 (MQ=255)
                                                                                       gCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTGTCACGATAACTGGTCAGCa                                                  >  1:467075/1‑143 (MQ=255)
                                                                                       gCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCa                                                  <  1:509889/143‑1 (MQ=255)
                                                                                              tCGCAGCAGTGTTGATGTAAGACAATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCTTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTgg                                           <  1:322599/143‑1 (MQ=17)
                                                                                              tCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTACCTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTGTgg                                           >  1:188632/1‑143 (MQ=255)
                                                                                              tCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACCTGCCGATGAATTACTCTCACGATAACTGCTCAGCCATTCTgc                                           >  1:375063/1‑142 (MQ=255)
                                                                                              tCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATAATCAAAATCTTTTACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTgg                                           <  1:239876/143‑1 (MQ=18)
                                                                                                                        acataAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATACCTGGTCAGCAATTCTGGCACATCTTGGTAAGCCCGACGAAccg                 >  2:131246/1‑141 (MQ=255)
                                                                                                                            aaaaaaGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAATCTCACGATAACTGGTAAGCAATTCTGGCCAATATTGGTAAGCCCGAAGCACTGGATa             >  2:268209/1‑143 (MQ=255)
                                                                                                                                       ggATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGt  >  1:489601/1‑143 (MQ=35)
                                                                                                                                       cgATACTTCCCATAAGCGCTAACTTAACGGTTGCGTTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGt  <  1:77319/142‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                       
AAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGT  >  NC_000913/2514131‑2514408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.