| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC JC | NC_000913 | 153,986 | IS5 (–) +4 bp | coding (1438‑1441/2598 nt) | htrE ← | putative outer membrane usher protein |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | 153986 = | 0 (0.000) | 52 (1.810) | 34/284 | 0.0 | 100% | coding (1441/2598 nt) | htrE | putative outer membrane usher protein |
| ? | NC_000913 | = 275149 | NA (NA) | noncoding (1/1195 nt) | IS5 | repeat region | |||||
| * | ? | NC_000913 | = 153989 | 0 (0.000) | 22 (0.760) | 19/284 | 0.5 | 100% | coding (1438/2598 nt) | htrE | putative outer membrane usher protein |
| ? | NC_000913 | 273955 = | NA (NA) | noncoding (1195/1195 nt) | IS5 | repeat region | |||||
GAAAGATAAAATGAACCGTAATCTTTTTTCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGG > NC_000913/153847‑154127 | gAAAGATAAAAGGAAGCGTAATCTCGTTTCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGAGTTTTCATGCGGGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTag < 2:515017/143‑1 (MQ=255)aaaatgtctttgctggtcggagggcgcttacgcctgaattcactgtcggcgaagctaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTag > 2:122144‑M1/140‑143 (MQ=255)aaaatctcttttctggtctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTag > 1:470680‑M1/140‑143 (MQ=255)aaaatctcttttctggtctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTag < 2:318042‑M1/4‑1 (MQ=255) aaacctcttttcctgtctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTaga > 1:155338‑M1/139‑143 (MQ=255) aatctcttttctggtctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTcgag > 2:90730‑M1/138‑143 (MQ=255) tctcttttctggtctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaacactgttctatggctcctgatgacaaacatgatctcatatcagggcattattcgcaccttccTTAAAGtt < 2:497612‑M1/8‑1 (MQ=255) ctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactaatgatgaacactgttctatggctccagatgacaaacatgatatcatatgagggacttgttcgcaacttacTTAGAGTTCGTGAATCATTaa < 2:47798‑M1/21‑1 (MQ=255) tgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctgcagatgctcaacctgatcccataacagggacctgttcgcaccgtccTTAGAGCTGGCGCAGCATTAAg < 2:499257‑M1/22‑1 (MQ=255) tgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGAGTTAGTGCATCATTAAg > 1:147148‑M1/122‑143 (MQ=255) tgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatgcctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGAGTTAGTGCCTCATTAAg < 2:222477‑M1/22‑1 (MQ=255) tCTGTTTTCTCAAAGTGCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGggaaggtgcgaataagcgggg < 1:521044‑M1/143‑22 (MQ=255) cgcttactggtgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGAGTTAGTGCATCATTAAGACCAAg > 2:37317‑M1/116‑143 (MQ=255) ttCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCCATTAGggaaggtgcgaataagcggggaaattc > 1:104527‑M1/1‑116 (MQ=255) tgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatctcaggcacttgttcgaaccttccTTAGAGTTAGTGCATCATTCAGACgaaggtgcttc < 2:320566‑M1/35‑6 (MQ=255) tgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGAGTTAGTGCACGCTTAAGACCAAGGCAATTc < 2:220423‑M1/35‑1 (MQ=255) tgctgaattcactgtcggcgaaggtaagttgatgacccatgatcaaccctgtcctatggctccagctgacaaacatgatctcatatcagggacttgctcgcaccttccTCAGAGTTAGTGCATCAGTAAGACCAAGGTAATTc < 2:286187‑M1/35‑1 (MQ=255) aaaTTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTGCATGCGTGAGTAATTACGCATGGATTTCGGTTGACGCTCTTGTTCGGGATGTCTCACTTCATCACTGCGggaaggtgcaaagaagcggtgaactgcttcta > 2:335545‑M1/1‑111 (MQ=255) ctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCtg < 2:330476‑M1/37‑1 (MQ=255) ctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGAGTTAGTGCATCATTAAGACCAAGGTAATCCtg < 1:427619‑M1/37‑1 (MQ=255) aaTTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGggaaggtgcgaataagcggggaaattcttctcg > 1:247910‑M1/1‑110 (MQ=255) tgaaggcactgtcggcggaggtgagttgatgactcatgaggaaccctgttctatggctccagatgacaaacatgatcgcatatcagggacttgttcgcaccttccTTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCtgt > 2:129454‑M1/106‑143 (MQ=255) gctacggTTTGTTAATATTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGCATTTCGGTTCAAGATCTTGTTCGGGATTTTTCACTTCATCAATTAGggaaggtgcgaataagcggggaaattcttctcggctg < 1:105691‑M1/140‑38 (MQ=255) tgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGCATAg < 2:409583‑M1/47‑1 (MQ=255) tgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAg > 2:148153‑M1/97‑143 (MQ=255) tggttAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGggaaggtgcgaataagcggggaaattcttctcggccgactcagtc > 2:99339‑M1/1‑98 (MQ=255) cggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGa > 1:246378‑M1/94‑143 (MQ=255) gttAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATGAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcat > 1:439494‑M1/1‑96 (MQ=255) gcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGAGTTAGTGCATCATTAAGACCCAGGTATTTCTGTGTCGAATAGCGatg < 1:26197‑M1/52‑2 (MQ=255) gaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggaattgttagcaccttccTTAGAGATAGTGCATAATTAAGAACAAGGTAGTTATGTGtcgaatagggaaaag < 2:197059‑M1/54‑8 (MQ=255) taagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGAGTTAGTGCATCATTAAGACCCAGGTAACTCTGTGTCGAATAGCGAGAGGCcgcg < 2:267614‑M1/59‑1 (MQ=255) tGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttc < 1:205178‑M1/143‑56 (MQ=255) ggaggactcgggatgaccccggttcgatggttcccggtggcaagcatgatctcatatcagggacttgttcgcaccttccTTAGAGTTAGTGCATCATTAAGAGCACGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATAtt > 2:224658‑M1/80‑143 (MQ=255) cTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGggaaggtgcgaataagcggggaagttcttctcggctggctcagtcatttcatttcttgatggttg > 1:44501‑M1/1‑78 (MQ=255) cTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttg > 1:441111‑M1/1‑78 (MQ=255) catgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCcgtgcag < 1:463452‑M1/72‑4 (MQ=255) tttttCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtgatttcgttgtttcatgtagagctg > 1:378816‑M1/1‑74 (MQ=255) ttttCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGggaaggtgcgaataagcggggaaattcttctgggatgactcagtaatttaatttgttcatgtgtgagcgg > 1:128019‑M1/1‑73 (MQ=255) gaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGAGGTAGGGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGGTGGTCAGTGAAGTGCTTg < 1:43940‑M1/78‑1 (MQ=255) cATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGggaaggtgcgaataagcggggaaactcttctcggctgactcagtcatttcatttcttcatgtcggagcccatct > 2:215489‑M1/1‑69 (MQ=255) aaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGt < 1:33267‑M1/79‑1 (MQ=255) aTGCGTAAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTTACTTCATCAATTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgatttt < 1:426807‑M1/143‑76 (MQ=255) tgcgtgcgTAATTACGCATGCATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGggaacgtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttt < 1:260331‑M1/143‑77 (MQ=255) gCGTGAGTAGTTTTGCATGGATTTCGGTGCGAGTTCTTGTTGGGGAGTTTCCACTTCATCAATTAGggaaggtgcgaataagcggggaaattcttctcggctgtctcagtcatttcatttcttcatgtttgagccgatttttt < 2:227370‑M1/143‑78 (MQ=255) cctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaacttccTTAGAGTTAGTGCATCATTAAGACCAAGGTCATTCTGTGTCGAATAGCGATAGGCCGCGATATTCACGGAAGTACttgtgtc < 1:169192‑M1/82‑4 (MQ=255) gttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGAGTTAGTGCATCATTAATACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTttcttc > 2:72785‑M1/59‑143 (MQ=255) aGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctccc < 2:419392‑M1/143‑83 (MQ=255) tggctccagatgacaaacatgatctcatatcagtcacttgttctcaccttccTTAGAGTTAGTGCATCATTAATACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATaa > 1:85429‑M1/53‑143 (MQ=255) tccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGAGTTAGTGCATCATTAAGACCCAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTg < 2:43900‑M1/95‑1 (MQ=255) atgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCa > 1:506599‑M1/44‑143 (MQ=255) aTTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaat > 1:295898‑M1/1‑46 (MQ=255) agaaacatggtgtcatatcagggacttgttcgcaccttccTTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGa > 2:138159‑M1/41‑143 (MQ=255) aaacatgatctcatatcagggacttgttcgcaccttccTTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGaaa < 1:28906‑M1/105‑1 (MQ=255) aaacatgatctcatatcagggacttgttcgcaccttccTTAGAGTTAGTGAATCATTAAGACCACGGTAATTCTGTGCCGAATCGCGATAGACCGCGATCTTCAGTGAAGTACTTGTTTCTTCGAATAACTAGCTCCAGGaaa < 2:184108‑M1/105‑1 (MQ=255) acatgatctcatatcagggacttgttcgcaccttccTTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAaca > 1:47798‑M1/37‑143 (MQ=255) cAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgatttttgctcccgtaaatgccttgaatcagcctatt < 1:223036‑M1/143‑107 (MQ=255) cAAGATCTTGGTCTGCATGTGTCACTTCATCAATTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatt < 2:277931‑M1/143‑107 (MQ=255) tctcatatcagggacttgttcgcaccttccTTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATaa > 2:185785‑M1/31‑143 (MQ=255) tatcagggacttgttcgcaccttccTTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGAAAACACGATAAATTTg < 2:492932‑M1/118‑1 (MQ=255) agggacttgttcgcaccttccTTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATCACTTGTTCCAGGAAACACGATAACTTTGcccc < 1:209252‑M1/122‑1 (MQ=255) ggacttgttcgcaccttccTTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGCCCGAGACCGTCGGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTg < 1:383041‑M1/124‑1 (MQ=255) ttgttcgcaccttccTTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTAt < 1:8609‑M1/128‑1 (MQ=255) tCACTTCATCAATTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccattt > 1:211357‑M1/1‑16 (MQ=255) cgcaccttccTTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTTGAATAGCTATAGTCTGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGtttt > 1:343647‑M1/11‑143 (MQ=255) cgcaccttccTTAAAGTTAGGGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGtttt > 1:489376‑M1/11‑143 (MQ=255) cttccTTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTatcat < 1:446034‑M1/138‑1 (MQ=255) cttccTTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGAGAACTTTGCCCCTGGTATGTTttatcgt < 2:327620‑M1/138‑3 (MQ=255) atcaATTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtgtcttcgccatttgaggcgt > 2:69067‑M1/1‑9 (MQ=255) cTTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGCTTTCTCatcccg < 2:17980‑M1/142‑3 (MQ=255) | GAAAGATAAAATGAACCGTAATCTTTTTTCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGG > NC_000913/153847‑154127 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |