Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	153,986	IS5 (–) +4 bp	coding (1438‑1441/2598 nt)	htrE ←	putative outer membrane usher protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	153986 =	0 (0.000)	52 (1.810)	34/284	0.0	100%	coding (1441/2598 nt)	htrE	putative outer membrane usher protein
	?	NC_000913	= 275149	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region
*	?	NC_000913	= 153989	0 (0.000)	22 (0.760)	19/284	0.5	100%	coding (1438/2598 nt)	htrE	putative outer membrane usher protein
	?	NC_000913	273955 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region

CCGGATGATAAAACATACCAGGGGCAAAGTTATCGTGTTTCCTGGAACAAGTTATTCGAAGAAACAAGTACTTCACTGAATATCGCGGCCTATCGCTATTCGACACAGAATTACCTTGGTCTTAATGATGCACTAACTCTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/154127‑153986 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTT  <  NC_000913/275149‑275008                                                                                                                                                                                                                                                                                              cgGGATGAGAAAGCATACCAGGGGCAAAGTTATCGTGTTTCCTGGAACAAGTTATTCGAAGAAACAAGTACTTCACTGAATATCGCGGCCTATCGCTATTCGACACAGAATTACCTTGGTCTTAATGATGCACTAACTCTAAG                                                                                                                                               <  2:17980/141‑1     acGATAAAACATACCAGGGGCAAAGTTCTCGTGTTTCCTGGAACAAGTTATTCGAAGAAACAAGTACTTCACTGAATATCGCGGCCTATCGCTATTCGACACAGAATTACCTTGGTCTTAATGATGCACTAACTCTAAGGAAG                                                                                                                                           <  2:327620/141‑1     ATGATAAAACATACCAGGGGCAAAGTTATCGTGTTTCCTGGAACAAGTTATTCGAAGAAACAAGTACTTCACTGAATATCGCGGCCTATCGCTATTCGACACAGAATTACCTTGGTCTTAATGATGCACTAACTCTAAGGAAG                                                                                                                                           <  1:446034/143‑1          AAAACATACCAGGGGCAAAGTTATCGTGTTTCCTGGAACAAGTTATTCGAAGAAACAAGTACTTCACTGAATATCGCGGCCTATCGCTATTCGACACAGAATTACCTTGGTCTTAATGATGCCCTAACTTTAAGGAAGGTGCG                                                                                                                                      >  1:489376/1‑143          AAAACATACCAGGGGCAAAGTTATCGTGTTTCCTGGAACAAGTTATTCGAAGAAACAAGTACTTCACTGAATATCGCAGACTATAGCTATTCAACACAGAATTACCTTGGTCTTAATGATGCACTAACTCTAAGGAAGGTGCG                                                                                                                                      >  1:343647/1‑143               ATACCAGGGGCAAAGTTATCGTGTTTCCTGGAACAAGTTATTCGAAGAAACAAGTACTTCACTGAATATCGCGGCCTATCGCTATTCGACACAGAATTACCTTGGTCTTAATGATGCACTAACTCTAAGGAAGGTGCGAACAA                                                                                                                                 <  1:8609/143‑1                   CAGGGGCAAAGTTATCGTGTTTCCTGGAACAAGTTATTCGAAGAAACAAGTACTTCACCGACGGTCTCGGGCTATCGCTATTCGACACAGAATTACCTTGGTCTTAATGATGCACTAACTCTAAGGAAGGTGCGAACAAGTCC                                                                                                                             <  1:383041/143‑1                     GGGGCAAAGTTATCGTGTTTCCTGGAACAAGTGATTCGAAGAAACAAGTACTTCACTGAATATCGCGGCCTATCGCTATTCGACACAGAATTACCTTGGTCTTAATGATGCACTAACTCTAAGGAAGGTGCGAACAAGTCCCT                                                                                                                           <  1:209252/143‑1                         CAAATTTATCGTGTTTTCTGGAACAAGTTATTCGAAGAAACAAGTACTTCACTGAATATCGCGGCCTATCGCTATTCGACACAGAATTACCTTGGTCTTAATGATGCACTAACTCTAAGGAAGGTGCGAACAAGTCCCTGATA                                                                                                                       <  2:492932/143‑1                              TTATCGTGTTTCCTGGAACAAGTTATTCGAAGAAACAAGTACTTCACTGAATATCGCGGCCTATCGCTATTCGACACAGAATTACCTTGGTCTTAATGATGCACTAACTCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGA                                                                                                                  >  2:185785/1‑143                                    TGTTTCCTGGAACAAGTTATTCGAAGAAACAAGTACTTCACTGAATATCGCGGCCTATCGCTATTCGACACAGAATTACCTTGGTCTTAATGATGCACTAACTCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGT                                                                                                            >  1:47798/1‑143                                      TTTCCTGGAGCTAGTTATTCGAAGAAACAAGTACTTCACTGAAGATCGCGGTCTATCGCGATTCGGCACAGAATTACCGTGGTCTTAATGATTCACTAACTCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTT                                                                                                          <  2:184108/143‑1                                      TTTCCTGGAACAAGTTATTCGAAGAAACAAGTACTTCACTGAATATCGCGGCCTATCGCTATTCGACACAGAATTACCTTGGTCTTAATGATGCACTAACTCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTT                                                                                                          <  1:28906/143‑1                                        TCCTGGAACAAGTTATTCGAAGAAACAAGTACTTCACTGAATATCGCGGCCTATCGCTATTCGACACAGAATTACCTTGGTCTTAATGATGCACTAACTCTAAGGAAGGTGCGAACAAGTCCCTGATATGACACCATGTTTct                                                                                                        >  2:138159/1‑141                                           TGGAACAAGTTATTCGAAGAAACAAGTACTTCACTGAATATCGCGGCCTATCGCTATTCGACACAGAATTACCTTGGTCTTAATGATGCACTAACTCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCAT                                                                                                     >  1:506599/1‑143                                                CAAGTTATTCGAAGAAACAAGTACTTCACTGAATATCGCGGCCTATCGCTATTCGACACAGAATTACCTGGGTCTTAATGATGCACTAACTCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGA                                                                                                <  2:43900/143‑1                                                    TTATTCGAAGAAACAAGTACTTCACTGAATATCGCGGCCTATCGCTATTCGACACAGAATTACCTTGGTATTAATGATGCACTAACTCTAAGGAAGGTGAGAACAAGTGACTGATATGAGATCATGTTTGTCATCTGGAGCCA                                                                                            >  1:85429/1‑143                                                          GAAGAAACAAGTACTTCACTGAATATCGCGGCCTATCGCTATTCGACACAGAATTACCTTGGTATTAATGATGCACTAACTCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAAC                                                                                      >  2:72785/1‑143                                                             gacACAAGTACTTCCGTGAATATCGCGGCCTATCGCTATTCGACACAGAATGACCTTGGTCTTAATGATGCACTAACTCTAAGGAAGTTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGG                                                                                   <  1:169192/140‑1                                                                ACAAGTACTTCACTGAATATCGCGGCCTATCGCTATTCGACACAGAATTACCTTGGTCTTAATGATGCACTAACTCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTT                                                                                <  1:33267/143‑1                                                                 CAAGCACTTCACTGACCACCGCGGCCTATCGCTATTCGACACAGAATTACCTTGGTCTTAATGATGCCCTACCTCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTC                                                                               <  1:43940/143‑1                                                                       ctgCACGGAATATCGCGGCCTATCGCTATTCGACACAGAATTACCTTGGTCTTAATGATGCACTAACTCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATG                                                                         <  1:463452/140‑1                                                                               AATATCGCGGCCTATCGCTATTCGACACAGAATTACCGTGCTCTTAATGATGCACTAACTCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGCCACCGGGAACCATCGAACCGGGGTCATCCCGAGTCCTCc                                                                 >  2:224658/1‑142                                                                                    CGCGGCCTCTCGCTATTCGACACAGAGTTACCTGGGTCTTAATGATGCACTAACTCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTA                                                            <  2:267614/143‑1                                                                                         cttttccCTATTCGACACATAACTACCTTGTTCTTAATTATGCACTATCTCTAAGGAAGGTGCTAACAATTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTC                                                       <  2:197059/136‑1                                                                                           cATCGCTATTCGACACAGAAATACCTGGGTCTTAATGATGCACTAACTCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGC                                                     <  1:26197/142‑1                                                                                             TCGCTATTCGACACAGAATTACCTTGGTCTTAATGATGCACTAACTCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCG                                                   >  1:246378/1‑143                                                                                                CTATTCGACACAGAATTACCTTGGTCTTAATGATGCACTAACTCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACA                                                >  2:148153/1‑143                                                                                                CTATGCGACACAGAATTACCTTGGTCTTAATGATGCACTAACTCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACA                                                <  2:409583/143‑1                                                                                                         ACAGAATTACCTTGGTCTTAATGATGCACTAACTCTAAGGAAGGTGCGAACAAGTCCCTGATATGCGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCCTCATGAGTCATCAACTCACCTCCGCCGACAGTGCCTTCA                                       >  2:129454/1‑143                                                                                                          CAGGATTACCTTGGTCTTAATGATGCACTAACTCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAG                                      <  1:427619/143‑1                                                                                                          CAGAATTACCTTGGTCTTAATGATGCACTAACTCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAG                                      <  2:330476/143‑1                                                                                                            gaagcACCTTCGTCTGAATGATGCACTAACTCTAAGGAAGGTTCGAACAAGTGCCTGAGATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCA                                    <  2:320566/138‑1                                                                                                            GAATTGCCTTGGTCTTAAGCGTGCACTAACTCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCA                                    <  2:220423/143‑1                                                                                                            GAATTACCTTGGTCTTACTGATGCACTAACTCTGAGGAAGGTGCGAGCAAGTCCCTGATATGAGATCATGTTTGTCAGCTGGAGCCATAGGACAGGGTTGATCATGGGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCA                                    <  2:286187/143‑1                                                                                                                   CTTGGTCTTAATGATGCACTAACTCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCACCAGTAAGCG                             >  2:37317/1‑143                                                                                                                         CTTAATGCTGCGCCAGCTCTAAGGACGGTGCGAACAGGTCCCTGTTATGGGATCAGGTTGAGCATCTGCAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCA                       <  2:499257/143‑1                                                                                                                         CTTAATGATGCACTAACTCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCA                       >  1:147148/1‑143                                                                                                                         CTTAATGAGGCACTAACTCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGGCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCA                       <  2:222477/143‑1                                                                                                                          TTAATGATTCACGAACTCTAAGTAAGTTGCGAACAAGTCCCTCATATGATATCATGTTTGTCATCTGGAGCCATAGAACAGTGTTCATCATTAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAG                      <  2:47798/143‑1                                                                                                                                       AACTTTAAGGAAGGTGCGAATAATGCCCTGATATGAGATCATGTTTGTCATCAGGAGCCATAGAACAGTGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGA         <  2:497612/143‑1                                                                                                                                        aaaacAAGGAAGGGGCGAACAAGTCCAAGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACGTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGAT        <  1:279788/138‑1                                                                                                                                         CTCGAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATT       >  2:90730/1‑143                                                                                                                                          agTAAGTAAGGTGCGAACAAGCCTCTGATATGAGATCATGTTTGTCATCTGGAGCCAGAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTTAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTT      <  2:36828/141‑1                                                                                                                                          TCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACAGGAAAAGAGGTTT      >  1:155338/1‑143                                                                                                                                           CTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTAGCTTCGCCGACAGTGAATTCAGGCGTAAGCGCCCTCCGACCAGCAAAGACATTTT     >  2:122144/1‑143                                                                                                                                           CTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTT     <  2:318042/143‑1                                                                                                                                           CTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTT     >  1:470680/1‑143                                                                                                                                            TAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTC    <  2:69394/143‑1                                                                                                                                            TAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATCTTC    >  1:226562/1‑143                                                                                                                                              AGGGAGGTTCGAACAAGGCCCTGATATGAGATCATGTTTGCCATCTGGAGCCATAGAACAGGGTTCATCATGAGACATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGTAAAGAGATTTTCTT  <  1:37868/143‑1                                                                                                                                              AGGAAGGTGCGAACAAGTCCCTGAGATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTT  <  2:406892/143‑1                                                                                                                                                                                                                                                                                              CCGGATGATAAAACATACCAGGGGCAAAGTTATCGTGTTTCCTGGAACAAGTTATTCGAAGAAACAAGTACTTCACTGAATATCGCGGCCTATCGCTATTCGACACAGAATTACCTTGGTCTTAATGATGCACTAACTCTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/154127‑153986 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTT  <  NC_000913/275149‑275008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.