Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	4,001,645	Δ5 bp	coding (220‑224/951 nt)	corA →	magnesium/nickel/cobalt transporter

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	4001645	4001649	5	37 [0]	[0] 36	corA	magnesium/nickel/cobalt transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4001644	0 (0.000)	36 (0.980)	30/284	0.2	100%	coding (219/951 nt)	corA	magnesium/nickel/cobalt transporter
	?	NC_000913	4001650 =	0 (0.000)					coding (225/951 nt)	corA	magnesium/nickel/cobalt transporter

GATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGT  >  NC_000913/4001509‑4001779                                                                                                                                              |                                                                                                                                  gtatggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttgtttgacgacgacgccggcctgcgtct                                                                                                                                  >  1:317691‑M2/142‑143 (MQ=255)   atggattgttcgtgtcgaaccggacgccgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTcc                                                                                                                                <  2:437741‑M2/4‑1 (MQ=255)             cttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttcttcttct                                                                                                                      >  1:399473‑M2/130‑143 (MQ=255)              ttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttcttcttctt                                                                                                                     <  1:282818‑M2/15‑1 (MQ=255)                gtcgaaccggacgacgacgagcgactgcgcgaacaatctgaacttggccagagcctggcaacccgcccggaactggaagtcatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTg                                                                                                                   <  1:251415‑M2/17‑1 (MQ=255)                    aaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGa                                                                                                               >  1:54075‑M2/123‑143 (MQ=255)                    aaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcacCTCCTTCTTCTTCTTTGAAGa                                                                                                               >  1:201433‑M2/123‑143 (MQ=255)                           cgcctccgatcgactgcgcgtacaatctgaacttggccagagcctggccacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGaa                                                                                                        <  2:17040‑M2/28‑1 (MQ=255)                            gacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgcagcatcggcacgtttctttgaagacgacgacggactgcatCTACTTCTTCATCTTTGGAGATGCGGGAg                                                                                                       >  2:129478‑M2/115‑143 (MQ=255)                                   agcgactgcgcgtacaatccgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGc                                                                                                <  1:595086‑M2/36‑1 (MQ=255)                                     cgaccgcgcgcacacgctgcacgtggcccgagcctggcaacccgctcggaactggaagacatcgaagcagcggcacgtttcgttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCg                                                                                              <  2:496205‑M2/38‑1 (MQ=255)                                       actgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGt                                                                                            >  2:437343‑M2/104‑143 (MQ=255)                                            gcgtacaatctgaacttggccagagcctggcaaccggcccgtatgtgggagacatcgaagcatcggcacgtttctttgacgacgacgacggcctgcatCTCCTTCTGCTTCGTTGAAGATGCGGAAGATCACGCAGGTAACTc                                                                                       >  1:315139‑M2/99‑143 (MQ=255)                                                   gtctggactgggccagagcctggcaacccgcccggaagtggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGAGCACGCCGGTAACTCCACtgtg                                                                                <  2:538200‑M2/52‑1 (MQ=255)                                                           ttggccagggcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTGCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTAc                                                                        <  1:77837‑M2/60‑1 (MQ=255)                                                           ttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTAc                                                                        <  1:444808‑M2/60‑1 (MQ=255)                                                            tggccagagcctggcaacccggccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTAcc                                                                       <  2:276496‑M2/61‑1 (MQ=255)                                                               ccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATc                                                                    >  1:99387‑M2/80‑143 (MQ=255)                                                               ccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACGGTGGCATTTACAATc                                                                    >  1:320568‑M2/80‑143 (MQ=255)                                                                      ctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGCGATg                                                             >  1:255347‑M2/73‑143 (MQ=255)                                                                        ggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGCTCACGCCGCTACCTCCACCGTGCCACTTACCCTCCGTGctgtc                                                           >  1:221661‑M2/71‑141 (MQ=255)                                                                             cccgcccggaactggaagacatcgaagcagcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCt                                                      <  1:179085‑M2/78‑1 (MQ=255)                                                                                    ggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTAct                                               <  2:317691‑M2/85‑1 (MQ=255)                                                                                    ggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTAct                                               <  1:583238‑M2/85‑1 (MQ=255)                                                                                        ctggaagacatcgaagcatcggcacggttcgttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgc                                           <  1:517594‑M2/89‑1 (MQ=255)                                                                                          ggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgcgt                                         <  1:576399‑M2/91‑1 (MQ=255)                                                                                              gacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgcgtgagc                                     <  1:322968‑M2/95‑1 (MQ=255)                                                                                              gacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgcgtgagc                                     <  1:322963‑M2/95‑1 (MQ=255)                                                                                                             gcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGACCTGCCCGCtt                      >  1:117587‑M2/34‑143 (MQ=255)                                                                                                                    tctttgaagacgacgacggcatgcatCTCCTTCTTCTTCTTTGAAGATGCAGAATATCACGCCGGTAACTCCACTGTAGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCt               <  1:11346‑M2/117‑1 (MQ=255)                                                                                                                       ttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTAGTCTGCGGGAGGGTGAAGCGCCGGCGTTGCGTatgga            >  2:615272‑M2/24‑141 (MQ=255)                                                                                                                          aagccgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCg         <  1:486111‑M2/123‑1 (MQ=255)                                                                                                                           agacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGt        >  2:180525‑M2/20‑143 (MQ=255)                                                                                                                           agacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGt        <  1:575010‑M2/124‑1 (MQ=255)                                                                                                                              cgacgacggcctgcatCTCCTCCTCCTTCTTTGAAGATGCGGAAGATCCCGCCGGTACCTCCCCTGTGCCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATg     <  1:205009‑M2/127‑1 (MQ=255)                                                                                                                                 cgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGt  >  1:172010‑M2/14‑143 (MQ=255)                                                                                                                                              |                                                                                                                                  GATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGT  >  NC_000913/4001509‑4001779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑