Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 4,001,645 Δ5 bp coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 4001645 4001649 5 37 [0] [0] 36 corA magnesium/nickel/cobalt transporter

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 40016440 (0.000)36 (0.980) 30/284 0.2 100% coding (219/951 nt) corA magnesium/nickel/cobalt transporter
?NC_000913 4001650 = 0 (0.000)coding (225/951 nt) corA magnesium/nickel/cobalt transporter

GATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGT  >  NC_000913/4001509‑4001779
                                                                                                                                             |                                                                                                                                 
gtatggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttgtttgacgacgacgccggcctgcgtct                                                                                                                                  >  1:317691‑M2/142‑143 (MQ=255)
  atggattgttcgtgtcgaaccggacgccgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTcc                                                                                                                                <  2:437741‑M2/4‑1 (MQ=255)
            cttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttcttcttct                                                                                                                      >  1:399473‑M2/130‑143 (MQ=255)
             ttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttcttcttctt                                                                                                                     <  1:282818‑M2/15‑1 (MQ=255)
               gtcgaaccggacgacgacgagcgactgcgcgaacaatctgaacttggccagagcctggcaacccgcccggaactggaagtcatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTg                                                                                                                   <  1:251415‑M2/17‑1 (MQ=255)
                   aaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGa                                                                                                               >  1:54075‑M2/123‑143 (MQ=255)
                   aaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcacCTCCTTCTTCTTCTTTGAAGa                                                                                                               >  1:201433‑M2/123‑143 (MQ=255)
                          cgcctccgatcgactgcgcgtacaatctgaacttggccagagcctggccacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGaa                                                                                                        <  2:17040‑M2/28‑1 (MQ=255)
                           gacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgcagcatcggcacgtttctttgaagacgacgacggactgcatCTACTTCTTCATCTTTGGAGATGCGGGAg                                                                                                       >  2:129478‑M2/115‑143 (MQ=255)
                                  agcgactgcgcgtacaatccgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGc                                                                                                <  1:595086‑M2/36‑1 (MQ=255)
                                    cgaccgcgcgcacacgctgcacgtggcccgagcctggcaacccgctcggaactggaagacatcgaagcagcggcacgtttcgttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCg                                                                                              <  2:496205‑M2/38‑1 (MQ=255)
                                      actgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGt                                                                                            >  2:437343‑M2/104‑143 (MQ=255)
                                           gcgtacaatctgaacttggccagagcctggcaaccggcccgtatgtgggagacatcgaagcatcggcacgtttctttgacgacgacgacggcctgcatCTCCTTCTGCTTCGTTGAAGATGCGGAAGATCACGCAGGTAACTc                                                                                       >  1:315139‑M2/99‑143 (MQ=255)
                                                  gtctggactgggccagagcctggcaacccgcccggaagtggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGAGCACGCCGGTAACTCCACtgtg                                                                                <  2:538200‑M2/52‑1 (MQ=255)
                                                          ttggccagggcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTGCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTAc                                                                        <  1:77837‑M2/60‑1 (MQ=255)
                                                          ttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTAc                                                                        <  1:444808‑M2/60‑1 (MQ=255)
                                                           tggccagagcctggcaacccggccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTAcc                                                                       <  2:276496‑M2/61‑1 (MQ=255)
                                                              ccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATc                                                                    >  1:99387‑M2/80‑143 (MQ=255)
                                                              ccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACGGTGGCATTTACAATc                                                                    >  1:320568‑M2/80‑143 (MQ=255)
                                                                     ctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGCGATg                                                             >  1:255347‑M2/73‑143 (MQ=255)
                                                                       ggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGCTCACGCCGCTACCTCCACCGTGCCACTTACCCTCCGTGctgtc                                                           >  1:221661‑M2/71‑141 (MQ=255)
                                                                            cccgcccggaactggaagacatcgaagcagcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCt                                                      <  1:179085‑M2/78‑1 (MQ=255)
                                                                                   ggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTAct                                               <  2:317691‑M2/85‑1 (MQ=255)
                                                                                   ggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTAct                                               <  1:583238‑M2/85‑1 (MQ=255)
                                                                                       ctggaagacatcgaagcatcggcacggttcgttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgc                                           <  1:517594‑M2/89‑1 (MQ=255)
                                                                                         ggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgcgt                                         <  1:576399‑M2/91‑1 (MQ=255)
                                                                                             gacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgcgtgagc                                     <  1:322968‑M2/95‑1 (MQ=255)
                                                                                             gacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgcgtgagc                                     <  1:322963‑M2/95‑1 (MQ=255)
                                                                                                            gcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGACCTGCCCGCtt                      >  1:117587‑M2/34‑143 (MQ=255)
                                                                                                                   tctttgaagacgacgacggcatgcatCTCCTTCTTCTTCTTTGAAGATGCAGAATATCACGCCGGTAACTCCACTGTAGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCt               <  1:11346‑M2/117‑1 (MQ=255)
                                                                                                                      ttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTAGTCTGCGGGAGGGTGAAGCGCCGGCGTTGCGTatgga            >  2:615272‑M2/24‑141 (MQ=255)
                                                                                                                         aagccgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCg         <  1:486111‑M2/123‑1 (MQ=255)
                                                                                                                          agacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGt        >  2:180525‑M2/20‑143 (MQ=255)
                                                                                                                          agacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGt        <  1:575010‑M2/124‑1 (MQ=255)
                                                                                                                             cgacgacggcctgcatCTCCTCCTCCTTCTTTGAAGATGCGGAAGATCCCGCCGGTACCTCCCCTGTGCCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATg     <  1:205009‑M2/127‑1 (MQ=255)
                                                                                                                                cgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGt  >  1:172010‑M2/14‑143 (MQ=255)
                                                                                                                                             |                                                                                                                                 
GATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGT  >  NC_000913/4001509‑4001779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.