breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 168,066 | A→T | K195* (AAA→TAA) | fhuA → | ferrichrome outer membrane transporter |
MC JC | 257,908 | Δ776 bp | [crl] | [crl] | |
JC JC | 911,086 | IS2 (–) +5 bp | intergenic (‑37/+92) | poxB ← / ← hcr | pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent |
RA | 1,159,109 | A→G | N414S (AAC→AGC) | ptsG → | fused glucose‑specific PTS enzymes: IIB component/IIC component |
JC JC | 1,293,032 | IS1 (–) +8 bp | intergenic (‑110/‑488) | hns ← / → tdk | global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase |
MC JC | 1,299,499 | Δ1,199 bp | intergenic (+254/‑485) | ychE → / → oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
MC JC | 1,978,503 | Δ776 bp | insB1–insA | insB1, insA | |
JC JC | 1,979,486 | IS5 (+) +4 bp | intergenic (‑271/‑264) | insA ← / → uspC | IS1 repressor TnpA/universal stress protein |
RA | 2,132,787 | A→C | I204S (ATC→AGC) | wcaA ← | putative glycosyl transferase |
RA | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
RA | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
RA | 3,799,134 | G→T | S60* (TCG→TAG) | waaU ← | lipopolysaccharide core biosynthesis |
MC JC | 3,815,859 | Δ82 bp | [rph]–[rph] | [rph], [rph] | |
RA | 3,963,061 | (G)5→4 | coding (1169/1485 nt) | gpp ← | guanosine pentaphosphatase/exopolyphosphatase |
MC JC | 4,001,645 | Δ5 bp | coding (220‑224/951 nt) | corA → | magnesium/nickel/cobalt transporter |
RA | 4,184,543 | C→A | P1100Q (CCG→CAG) | rpoB → | RNA polymerase, beta subunit |
RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
Unassigned missing coverage evidence | ||||||||||
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seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 123023 | 127553 | 4531 | 6 [5] | [2] 6 | [aceE]–[aceF] | [aceE],[aceF] |
* | * | ÷ | NC_000913 | 1612280 | 1612325 | 46 | 6 [5] | [5] 7 | yneG | DUF4186 family protein |
* | * | ÷ | NC_000913 | 2066277–2066464 | 2066588–2066466 | 3–312 | 7 [5] | [4] 6 | [insH1] | [insH1] |
* | * | ÷ | NC_000913 | 3423777–3424234 | 3424541–3424240 | 7–765 | 6 [5] | [5] 6 | [rrfD]–[rrlD] | [rrfD],[rrlD] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 315563 = | NA (NA) | 5 (0.200) | 5/220 | 2.7 | NA | noncoding (335/1255 nt) | IS3 | repeat region |
? | NC_000913 | 315599 = | NA (NA) | noncoding (371/1255 nt) | IS3 | repeat region | |||||
* | ? | NC_000913 | 521820 = | 21 (0.750) | 6 (0.230) | 5/222 | 2.7 | 23.3% | coding (1405/2415 nt) | ybbP | putative ABC transporter permease |
? | NC_000913 | 521868 = | 20 (0.780) | coding (1453/2415 nt) | ybbP | putative ABC transporter permease | |||||
* | ? | NC_000913 | 1207790 = | 0 (0.000) | 14 (0.580) | 13/208 | 0.9 | 93.3% | coding (290/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913 | 1209619 = | 2 (0.080) | pseudogene (1/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | NC_000913 | = 1207805 | 0 (0.000) | 30 (1.240) | 25/208 | 0.1 | 96.8% | coding (305/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913 | = 1209602 | 2 (0.080) | pseudogene (18/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related |