Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,159,109	A→G	N414S (AAC→AGC)	ptsG →	fused glucose‑specific PTS enzymes: IIB component/IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,159,109	0	A	G	100.0%	118.4 / NA	34	N414S (AAC→AGC)	ptsG	fused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/16);  total (18/16)

GATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTA  >  NC_000913/1158963‑1159252                                                                                                                                                   |                                                                                                                                                gATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCa                                                                                                                                               >  6:45257/1‑149 (MQ=255)   ttGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCAtt                                                                                                                                             <  5:45257/149‑1 (MQ=255)   ttGTTTACTACAACATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCAtt                                                                                                                                             <  8:38875/149‑1 (MQ=255)                                            gATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTa                                                                                                                                            >  6:112643/1‑109 (MQ=255)                                                                         gACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAt                                                                              <  4:18344/142‑1 (MQ=255)                                                                         gACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAt                                                                              >  3:18344/1‑142 (MQ=255)                                                                           cTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAggccggc                                                                     <  1:111652/149‑1 (MQ=255)                                                                            tGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAggccggcc                                                                    >  1:35561/1‑149 (MQ=255)                                                                                                gTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATgtgt                                                                                         <  3:26661/108‑1 (MQ=255)                                                                                                gTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATgtgt                                                                                         >  4:26661/1‑108 (MQ=255)                                                                                                     aGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCTGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAggc                                                                         >  5:113461/1‑119 (MQ=255)                                                                                                     aGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCTGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAggc                                                                         <  6:113461/119‑1 (MQ=255)                                                                                                     aGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCa                                                                                                        <  8:62071/88‑1 (MQ=255)                                                                                                     aGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCa                                                                                                        >  7:62071/1‑88 (MQ=255)                                                                                                      gCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGAc                                                                                                                                 <  8:73799/62‑1 (MQ=255)                                                                                                      gCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGAc                                                                                                                                 >  7:73799/1‑62 (MQ=255)                                                                                                      gCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCg                                            >  7:43670/1‑147 (MQ=255)                                                                                                         aaaTGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTg                                       >  6:133172/1‑149 (MQ=255)                                                                                                              gCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGc                                  >  6:18560/1‑149 (MQ=255)                                                                                                                    gCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTgg                                      >  4:11613/1‑139 (MQ=255)                                                                                                                    gCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCAGGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTgg                                      <  3:11613/139‑1 (MQ=255)                                                                                                                      gctGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTg                          <  8:43670/148‑1 (MQ=255)                                                                                                                        tGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTg                          <  5:133172/147‑1 (MQ=255)                                                                                                                               tgcATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAgg                 >  4:115908/1‑149 (MQ=255)                                                                                                                                 cATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGt                                                                                  >  1:77426/1‑82 (MQ=255)                                                                                                                                 cATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGt                                                                                  <  2:77426/82‑1 (MQ=255)                                                                                                                                         ggtAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGt                                                                                  <  7:65770/74‑1 (MQ=255)                                                                                                                                         ggtAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGt                                                                                  >  8:65770/1‑74 (MQ=255)                                                                                                                                          gtAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCgg       <  5:18560/148‑1 (MQ=255)                                                                                                                                             aaGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAggccggc                                                                     >  7:58957/1‑83 (MQ=255)                                                                                                                                             aaGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAggccggc                                                                     <  8:58957/83‑1 (MQ=255)                                                                                                                                               gAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGgtg                       >  1:110920/1‑127 (MQ=255)                                                                                                                                               gAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGgtg                       <  2:110920/127‑1 (MQ=255)                                                                                                                                               gAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTa  >  8:72237/1‑148 (MQ=255)                                                                                                                                                   |                                                                                                                                                GATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTA  >  NC_000913/1158963‑1159252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑