Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	CP009273	4,145,501	Δ320 bp	coding (573‑892/1005 nt)	argC →	N‑acetyl‑gamma‑glutamylphosphate reductase, NAD(P)‑binding

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	CP009273	4145501	4145820	320	61 [0]	[0] 61	argC	N‑acetyl‑gamma‑glutamylphosphate reductase, NAD(P)‑binding

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	= 4145500	0 (0.000)	57 (1.090)	45/278	0.2	100%	coding (572/1005 nt)	argC	N‑acetyl‑gamma‑glutamylphosphate reductase, NAD(P)‑binding
	?	CP009273	4145821 =	0 (0.000)					coding (893/1005 nt)	argC	N‑acetyl‑gamma‑glutamylphosphate reductase, NAD(P)‑binding

AAATTAAAAGAAGCGAATTTGATTGCGGTGCCGGGCTGTTATCCGACGGCGGCACAGCTGGCGCTGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGTAAAGCGGCCATTTCAAACAGCTTTTGTGAAGTTAGCCTGCAACCGTATGGCGTCTTTACTCATCGCCATCAACCAGAGATCGCCACACACCTCGGTGCTGACGTTATCTTCACCCCACATCTGGGCAATTTCCCGCGCGGCATTCT  >  CP009273/4145352‑4145647                                                                                                                                                     |                                                                                                                                                    aaaTTAAAAGAAGCGAATTTGATTGCGGTGCCGGGCTGTTATCCGACGGCGGCACAGCTGGCGCTGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACTAGCGGGGTGAGCGGTGCAGGGCG                                                                                                                                                     >  1:904142‑M1/1‑149 (MQ=255)  aaTTAAAAGAAGCGAATTTGATTGCGGTGCCGGGCTGTTATCCGACGGCGGCACAGCTGGCGCTGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGa                                                                                                                                                    <  1:870824‑M1/149‑2 (MQ=255)    ttAAAAGAAGCGAATTTGATTGCGGTGCCGGGCTGTTATCCGACGGCGGCACAGCTGGCGCTGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGGTGTCTACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagc                                                                                                                                                  >  1:630208‑M1/1‑146 (MQ=255)     tAAAAGAAGCGAATTTGATTGCGGTGCCGGGCTGTTATCCGACGGCGGCACAGCTGGCGCTGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagca                                                                                                                                                 >  1:129003‑M1/1‑145 (MQ=255)         agaagCGGATTGGAGGGCGGGGCCGGGGTGGTATCCGACGGCGGCACAGCTGGCGCTGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAAGCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagc                                                                                                                                                  <  2:797298‑M1/144‑4 (MQ=255)              cGAATTTGATTGCGGTGCCGGGCTGTTACCAGACGGCGGCACAGCTGGCGCTGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattat                                                                                                                                        <  1:863528‑M1/149‑14 (MQ=255)                  tttGATTGCGGTGCCGGGCTGTTATCCGACGGCGGCACAGCTGGCGCTGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtg                                                                                                                                    <  1:539012‑M1/149‑18 (MQ=255)                  tttGATTCCGGTGCCGGGCTGTTATCCGACGGTGGCACAGCTGGCGCTGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtg                                                                                                                                    <  1:474031‑M1/149‑18 (MQ=255)                     gATTGCGGTGCCGGGCTGTTATCCGACGGCGGCACAGCTGGCGCTGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgcttattg                                                                                                                                      <  2:542065‑M1/144‑16 (MQ=255)                       ttGCGGTGCCAGGCTGTAAACCGAAAGCGGAACAGCTGGAGCTAAAACCATTGATTGATGCCGATCTTCTTGACCTCACTCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCTGTGCAGGGCGagcatctgattattgtggcgac                                                                                                                               <  1:886973‑M1/149‑23 (MQ=255)                              gCCGGGCTGTTATCCGACGGCGGCACAGCTGGCGCTGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccg                                                                                                                             <  2:314064‑M1/144‑25 (MQ=255)                               tcgGGCTGTTATCGGACGGCGGCACAGCTGGCGCTGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagaca                                                                                                                       <  1:53500‑M1/148‑31 (MQ=255)                                  ggCTGTTATCCGACGGCGGCACAGCTGGCGCTGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaact                                                                                                                    <  1:128456‑M1/149‑34 (MQ=255)                                  ggCTGTTATCCGACGGCGGCACAGCTGGCGCTGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaaga                                                                                                                         <  2:315023‑M1/144‑29 (MQ=255)                                   gCTGTTATCCGACGGCGGCACAGCTGGCGCTGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagac                                                                                                                        <  2:263625‑M1/144‑30 (MQ=255)                                    cTGTTATCCGACGGCGGCACAGCTGGCGCTGAAACCGTTGATTGATGCCGATCTTCTTGCCCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaaatta                                                                                                                  >  1:243885‑M1/1‑114 (MQ=255)                                     tGTTATCCGACGGCGGCACAGCTGGCGCGGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCCACGCAACGAGCGGCATGCATGATCCAGGGCGagcatctgattattgtgtcgaccgaagacaacttac                                                                                                                 <  1:681010‑M1/149‑37 (MQ=255)                                          tCCGACGGCGGCACAGCTGGCGCTGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcgtctgagtattgtggcgaccgaaggcaacttac                                                                                                                 >  2:403826‑M1/1‑108 (MQ=255)                                                  cgggcccgcgggcgctgaaaccGTTGATTGATGCCGATCTCCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCACCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggc                                                                                                    <  1:67874‑M1/139‑50 (MQ=255)                                                    gcACAGCTGGCGCTGAAACCGTTGAATGATACCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCCACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcgg                                                                                                  >  1:481619‑M1/1‑98 (MQ=255)                                                     cacaGCTGGCGCTGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggc                                                                                                 >  1:927018‑M1/1‑97 (MQ=255)                                                       caGCTGGCGCTGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCCGTGGCCGGTGATCAACGCTACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggc                                                                                                    >  2:820076‑M1/1‑95 (MQ=255)                                                        aGCTGGCGCTGAAACCGTGGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcg                                                                                                   <  2:793425‑M1/144‑51 (MQ=255)                                                          cTGGCGCTGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggc                                                                                                 >  2:212597‑M1/1‑92 (MQ=255)                                                           tGGCGCTGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggcacaagc                                                                                           >  1:232664‑M1/1‑91 (MQ=255)                                                                cTGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgcaaggggcggcggcccaagc                                                                                           >  2:207386‑M1/1‑86 (MQ=255)                                                                 tGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCCGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggcacaagcg                                                                                          <  2:840553‑M1/144‑60 (MQ=255)                                                                 tGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagaaagtttttttgttggcgcggcggcacaagcggtaca                                                                                     <  1:225307‑M1/149‑65 (MQ=255)                                                                 tGAAACCGTTGATCGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggcacaagcggtaca                                                                                     <  1:268003‑M1/149‑65 (MQ=255)                                                                 cgaAACCGCTGATTGATGCCTATCTTCTCGCCCTCAATCAGTGGCCGGTGACCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgcacggtctcaaccgactgaaaggcgcggcggcacaagcggtaca                                                                                     <  1:224156‑M1/148‑65 (MQ=255)                                                                     aCCGTTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggcacaagcggtacagtgc                                                                                 >  1:462053‑M1/1‑81 (MQ=255)                                                                              tgatGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggcacaagcggtacagtgcgccaatatt                                                                        >  1:925896‑M1/1‑72 (MQ=255)                                                                              tgatGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggcacaagcggtacagtgcgccaatatt                                                                        >  1:608869‑M1/1‑72 (MQ=255)                                                                                          tcttGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggcacaagcggtacagtgcgccaatattcgtttcg                                                                 <  2:418872‑M1/144‑85 (MQ=255)                                                                                             tgactcctatcagtggcCGGTGATCAACCCCACCACGGGCGTGGGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggcacaagcggtacagtgcgccaatattcgtttcggct                                                              <  2:124483‑M1/136‑88 (MQ=255)                                                                                                ccTCCATCAGGGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgatcattgtggcgaccgaagacaacttactgaaaggcgcggcggcacaagcggtacagtgcgccaatattcgtttcggctatg                                                           <  2:113610‑M1/144‑91 (MQ=255)                                                                                                 cTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaacgaagacaacttactgaaaggcgcggcggcaccagcggtgcagtgcgccaatatggcttgcggctatgg                                                          >  2:32895‑M1/1‑53 (MQ=255)                                                                                                  tCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggcacaagcggtacagtgcgccaatattcgtttcggctatgcggaaac                                                    >  1:603677‑M1/1‑52 (MQ=255)                                                                                                  tCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggagcggcggcacaaccggtacagccggccagtccccgtgtcggctatgcggggag                                                    >  1:398019‑M1/1‑52 (MQ=255)                                                                                                  ccagtcggggtccggtgatcaacgCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttaccgaaaggcgcggcggcacaagcggtacagtgcgccaatattcgtttcggctatgcg                                                         <  2:537209‑M1/133‑93 (MQ=255)                                                                                                   cAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggcacaagcggtacagtgcgccaatattcgtttcggctatgcgg                                                        <  2:472433‑M1/144‑94 (MQ=255)                                                                                                          tGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacgtagtgaagggcaggacggcacaagcggtacagtgcgccaatattcgtttcggctatgcggaaacgccgtctc                                            >  1:445666‑M1/1‑44 (MQ=255)                                                                                                             ccGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggcacaagcggtacagtgcgccaatattcgtttcggctatgcggaaacgcagtctctta                                         >  1:537443‑M1/1‑41 (MQ=255)                                                                                                              cGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggcacaagcggtacagtgcgccaacatgcggttcggctatgcggaaactcagtcg                                             >  2:472095‑M1/1‑40 (MQ=255)                                                                                                                  gATCAAGGCGACCAGCGGGGTGAGCGGTGCGGGGCGagcgtctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggcacaagcggtacagtgcgccaatattcgtttcggctatgcggaaacgcagtctctta                                         <  2:56506‑M1/144‑109 (MQ=255)                                                                                                                   aTCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggcacaagcggtacagtgcgccaatattcgtttcggctatgcggaaacgcaccactccgc                                        <  2:46886‑M1/144‑110 (MQ=255)                                                                                                                    tCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggcacaagcggtacagtgcgccaatattcgtttcggctatgcggaaacgcagtctcttatttaagg                                  >  1:855183‑M1/1‑34 (MQ=255)                                                                                                                    tCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgaggcgaccgaagacaacttactgaaaggcgcggcggcacaagcggtacagtgcgccaatattcgtttcggctatgcggaaacgcagtctcttatttaagg                                  >  1:663489‑M1/1‑34 (MQ=255)                                                                                                                      aaCGCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggcacaagcggtacagtgcgacaatactcggttcggctatgcggaaacgcagcctcctactga                                     >  2:506430‑M1/1‑32 (MQ=255)                                                                                                                      aaCGCCACCAGCGGCGTGAGCGGTGAAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggcacaagcggtacagtgcgccaatattcgttgcggctctgcggaaccgcagccccttctttaagggt                                >  1:554164‑M1/1‑32 (MQ=255)                                                                                                                         gCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggcacacgcggtgcagtgcgccaatattcgtttcggctatgaggaaaagcagtctcttatttaagggggca                             >  1:13679‑M1/1‑29 (MQ=255)                                                                                                                         gCCACCAGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggcacaagcggtacagtgcgccaatattcgtttcggctatgcggaaacgcagtctcttatttaagggtgga                             >  1:273686‑M1/1‑29 (MQ=255)                                                                                                                          ccaccaGCGGCGTGAGCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggcacaagcggtacagtgcgccaatattcgtttcggctatgcggaaacgcagtctcttatttaagggtgcaa                            >  1:802408‑M1/1‑28 (MQ=255)                                                                                                                                      tGAGCGGTTCAGGGCGaggatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggcacaagcggtacagtgcgccaatattcgtttcggctatgcggaaacgcagtctcttatttaagggtgcaatgatgaatccat                <  1:59077‑M1/149‑134 (MQ=255)                                                                                                                                         gCGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggcacaagcggtacagtgcgccaatcttcgtttcggctatgcggaaacgcagtctcttatttaagggtgcaatgatgaatccattaa             <  1:872792‑M1/149‑137 (MQ=255)                                                                                                                                          cGGTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggcacaagcggtacagtgcgccaatattcgtttcggctatgcggaaacgcagtctcttatttaagggtgcaatgatgaatacc                 >  2:368220‑M1/1‑12 (MQ=255)                                                                                                                                           ggTGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggcacaagcggtacagtgcgccaatattcgtttcggctatgcggaaacgcagtctcttatttaagggtgcaatgatgaatccat                <  2:521307‑M1/144‑134 (MQ=255)                                                                                                                                             tGCAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggcacaagcggtacagtgcgccaatattcgtttcggctatgcggcaacgcagtctcttatttaagggtgcaatgatgaatccattaagtat         >  1:767613‑M1/1‑9 (MQ=255)                                                                                                                                               cAGGGCGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggcacaagcggtacagtgcgccaatattcgtttcggctatgcggaaacgcagtctcttatttaagggtgcaatgatgaatccattaattatca       >  1:529659‑M1/1‑7 (MQ=255)                                                                                                                                                  ggCGggcatctgattattgtggcgaccgaagacaatttacagaaaggcgcggcggcacaagcggtacagtgcgccaatattcgtttcggctatgcggaaacgcagtctcttatttaagggtgcaatgatgaatccattaattat         <  2:88714‑M1/144‑141 (MQ=255)                                                                                                                                                    cGagcatctgattattgtggcgaccgaagacaacttactgaaaggcgcggcggcacaagcggtacagtgcgccaatattcgtttcgtctatgcggaaacgcagtctcttatttaagggtgcaatgatgaatcccttaattatcaaactg  >  1:627512‑M1/1‑2 (MQ=255)                                                                                                                                                     |                                                                                                                                                    AAATTAAAAGAAGCGAATTTGATTGCGGTGCCGGGCTGTTATCCGACGGCGGCACAGCTGGCGCTGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGTAAAGCGGCCATTTCAAACAGCTTTTGTGAAGTTAGCCTGCAACCGTATGGCGTCTTTACTCATCGCCATCAACCAGAGATCGCCACACACCTCGGTGCTGACGTTATCTTCACCCCACATCTGGGCAATTTCCCGCGCGGCATTCT  >  CP009273/4145352‑4145647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑