New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 1973060 =4 (0.060)56 (0.900) 40/292 0.7 93.3% intergenic (‑606/‑174) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 = 3577555 NA (NA)intergenic (+15/‑1) insB1/yrhA IS1 transposase B/pseudogene, interrupted by IS1E

ATTAAAGCTTTACTCCTAATGGAGAAACGACGCAATCCCAACTCGGTCAAACAGGTAAATATTAGTCTGATTATTAGTAAAGATAATTAATCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP009273/1973200‑1973060
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCG  <  CP009273/3577555‑3577415
                                                                                                                                                                                                                                                                                          
ATTAAAGCCTTACTCCTAATGGATAAACGGCGTAATCCCAAGTCGGTCAAACAGGTAAATATTAGTCTGATTATTAGTAAAGATAATTAATCAATCACTCCCGGTGATCATATAATTTCAAATGTTATTATAAGAAAATAGGGTAATGA                                                                                                                                       <  1:703661/149‑1
             TCCTAATGGAGAAACGACGCAATCCCAACTCGGTCAAACAGGTAAATATTAGTCTGATTATTAGTAAAGAGAATTAATCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCACCTTATTG                                                                                                                          >  1:765934/1‑149
                TAATTGAAAAACCACACAATCCCAACTCGGTCAAACATGTAAATATTACTCTGATTATTAGTAAAGATAATTAATCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATA                                                                                                                       <  1:214044/149‑1
                TAATGGAGAAACGACGCAATCCCAACTCGGTCAAACAGGTAAATATTAGTCTGATTATTAGTAAAGATAATTAATCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATA                                                                                                                       <  2:623606/149‑1
                TAATGGAGAAACGACGCAATCCCAACTCGGTCAAACAGGTAAATATTAGTCTGATTATTAGTAAAGATAATTAATCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGCGTAATGACTCCAACTTATTGATA                                                                                                                       <  2:565258/149‑1
                TAATGGAGAAACGACGCAATCCCAACTCGGTCAAACAGGTAAATATTAGTCTGATTATTAGTAAAGATAATTAATCAATCACTCCAGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATA                                                                                                                       >  2:330525/1‑149
                     GAGAAACGACGCAATCCCAACTCGGTCAAACAGGTAAATATTAGTCTGATTATTAGTAAAGATAATTAATCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATGGTGTT                                                                                                                  >  1:395690/1‑149
                     GAGAAACGACGCAATCCCAACTCGGTCAAACAGGTAAATATTAGTCTGATTACTAGTAAAGATAATTAATCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTT                                                                                                                  >  2:807672/1‑149
                       GAAACGACGCAATCCCAACTCGGTCAAACAGGTAAATATTAGTCTGATTATTAGTAAAGATAATTAATCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTT                                                                                                                <  2:456006/149‑1
                       GAAACGACGCAATCCCAACTCGGTCAAACAGGTAAATATTAGTCTGATTATTAGTAAAGATAATTAATCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTT                                                                                                                <  2:738544/149‑1
                       GAAAAGACGCAATCCCAACTCGGTCAAACATGTAAATATTAGTCTGATTATTAGTAAAGATAATTAATTAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTT                                                                                                                <  1:28367/149‑1
                          ACGACGCAATCCCAACTCGGTCAAACAGGTAAATATTAGTCTGATTATTAGTAAAGATAATTAATCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATG                                                                                                             <  2:944059/149‑1
                          ACGACGCAATCCCAACTCGGTCAAACAGGTAAATATTAGTCTGATTATTAGTAAAGATAATTAATCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATG                                                                                                             >  1:200872/1‑149
                               GCAATCCCAACTCGGTCAAACAGGTAAATATTAGTCTGATTATTAGTAAAGATAATTAATCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAG                                                                                                        >  2:696070/1‑149
                                    CCCGACTCGGTCAAACAGGTAAATATTAGTCTGATTATTAGTAAAGATCATTAATTAATCACTCCCGGTGATCATATAATTTCAACTGTAATTATAAGGAAATAGGGTAATGCTTCCAACTTATTGATAGTGTTTTATGTTCAGATAAT                                                                                                   <  1:117687/149‑1
                                      CAACTCGATCAAACAGGTAAATATTAGTCTGATTATTAGTAAAGATAATTAATCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGC                                                                                                 <  2:778370/149‑1
                                          tcgtgCAAACAGGTAAATATTAGGCTGATTATTAGGAATGATAATTAATCAATCAATCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGA                                                                                             <  1:852722/144‑1
                                          TCGGTCAAACAGGTAAATATTAGTCTGATTATTAGTAAAGATAATTAATCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGA                                                                                             <  2:990293/149‑1
                                              TCAAACAGGTAAATATTAGTCTGATTATTAGTAAAGATAATTAATCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATGCGAAAGTAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGAC                                                                                         >  2:1016150/1‑149
                                                AAACAGGTAAATATTAGTCTGATTATTAGTAAAGATAATTAATCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTT                                                                                       >  2:144999/1‑149
                                                 aacctggAAATATTCGTGTGATTATTAGTAAAGATTATTAATCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTT                                                                                      <  1:547317/142‑1
                                                 AACATGTAAATTTTATTCTTATTATTAGTAAAGATAATTAATCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTT                                                                                      <  1:416874/149‑1
                                                 AACAGGTAAACATTAGTCTGATTATTAGTAAAGATAATTAATCAATCACTCCCGGTTATCATATAATTTCAAATGTAAGTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTT                                                                                      <  1:582232/149‑1
                                                        AAATATTAGTCTGATTATTAGTAAAGATAATTAATCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGC                                                                               >  2:962664/1‑149
                                                               agttttagTATTAGTAAAGATAATTAATCAATCACTCCCGGTGATCATATAATTTCAAATGTAATCATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCA                                                                        <  1:769895/141‑1
                                                               AGTCTGATTATTAGTAAAGATAATTAATCAATCACTCCCGGGGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAAATTATCGCTAGTGTTTTATTTTCAGATAATGCCCGATAAATTTGTCATGCAGCACCc                                                                        >  1:55396/1‑148
                                                                GTCTGATTATTAGTAAAGATAATTAATCAATCACTCCCGTTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGACGACTTTGTCATGCAGCTCCAC                                                                       <  2:732397/149‑1
                                                                  CTGATTATTAGTAAAGATAATTAATCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCG                                                                     >  2:420811/1‑149
                                                                   TGATTATTAGTAAAGATAATTAATCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGA                                                                    >  2:39564/1‑149
                                                                   TGATTATTAGTAAAGATAATTAATCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTAATAGTGTTTTATGTTTAGTTAATGACCGATGTCTTTGTCATGCAGCTCCACGGA                                                                    >  2:137608/1‑149
                                                                   TGATTATTAGTAAAGATAATTAAGCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATACAGCTCCACCGA                                                                    >  2:542260/1‑149
                                                                       TATTAGTAAAGATAATTAATCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTT                                                                >  2:170928/1‑149
                                                                       TATTAGTAAAGATAATTAATCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTT                                                                <  1:696070/149‑1
                                                                                 GATAATTAATCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGCCA                                                      >  2:326379/1‑149
                                                                                     ATTAATCAATCTCTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTTCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGA                                                  <  2:562377/149‑1
                                                                                         ATCAAGCCCTCCCGGTGATCATATAATTTCAAATGTAATTATAAGACAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTC                                              <  1:285753/149‑1
                                                                                          TCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCC                                             >  2:744478/1‑149
                                                                                          TCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCC                                             <  2:955272/149‑1
                                                                                            AATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTGCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGT                                           >  2:687047/1‑149
                                                                                            AATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGACTAAGAGAACGACAGCGTCTTCCGT                                           >  1:259259/1‑149
                                                                                                tCTCCCGGTTATCATATAATTTCAAATGTAATTATAAGAAAATAGGTTAATTACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCA                                       <  2:1022517/148‑1
                                                                                                ACTCCCGGAGATCGTGTAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATAGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGCTTTTGAGAACGACAGCGACTTCCGTCCCA                                       >  1:762403/1‑149
                                                                                                ACTCCCGAAGAGCACATAATTGCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAAAGCCCGATGACTTCGTCATGCACCTCCACCGATTTACAGAACCACAGCGACCTCCGTCCCA                                       <  1:7148/149‑1
                                                                                                  TCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGC                                     <  2:103410/149‑1
                                                                                                   cggtgtgagtATATTAGTTCAAATGTTATTATAAGAAAATAGGGGAATGATTCCAACTTATTGATAGTGTTTTATGTTCAGAGAATGCCCGATGACTTTGTAATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCC                                    <  1:303927/139‑1
                                                                                                     CGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGT                                  <  1:688673/149‑1
                                                                                                        TGATCATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCC                               >  2:643220/1‑149
                                                                                                            CATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGT                           <  1:144999/149‑1
                                                                                                             ATATAATTTCAAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTGCCGTCCCAGCCGTGCCAGATG                          >  1:333905/1‑149
                                                                                                                       AAATGTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTTTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGA                <  1:39564/149‑1
                                                                                                                           GTAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGCTGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGGCGTGCCAGCGGCTGCCCCCGATTCA            >  2:626335/1‑149
                                                                                                                            TAATTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAG           <  2:225101/149‑1
                                                                                                                               TTATAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTT        <  1:962664/149‑1
                                                                                                                                  TAAGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATG     >  1:208130/1‑149
                                                                                                                                    AGAAAATAGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCC   <  2:84047/149‑1
                                                                                                                                     aAAAATAGCGTACTGACTCCAACTTATTCATAGTGTTTTATGTTCAGATAATGCCCGATAACTTTGTCATGCAGCGCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTTCTTCAGATTCAGGTTATGCCG  <  1:631798/148‑1
                                                                                                                                                                                                                                                                                          
ATTAAAGCTTTACTCCTAATGGAGAAACGACGCAATCCCAACTCGGTCAAACAGGTAAATATTAGTCTGATTATTAGTAAAGATAATTAATCAATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP009273/1973200‑1973060
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCG  <  CP009273/3577555‑3577415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.