Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC CP009273 2,400,257 IS5 (+) +4 bp 50.0% intergenic (‑137/‑780) lrhA ← / → alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 = 2283592NA (NA)11 (0.620) 8/276 NT 54.3% noncoding (1/1195 nt) IS5 repeat region
?CP009273 = 2400260 9 (0.520)intergenic (‑140/‑780) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2
* ? CP009273 2400257 =9 (0.520)7 (0.410) 7/268 NT 43.8% intergenic (‑137/‑783) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2
?CP009273 3358915 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region

ATTTGGCCCGAATGGTTCATTAAATCTGATCGCCAGCGATGCATTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAA  >  CP009273/3358789‑3359043
                                                                                                                              |                                                                                                                                
atttggCCCGAATGGTTCATTAAATCTGATCGCCAGCGATGCATTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGaa                                                                                                            <  1:158015/149‑1 (MQ=255)
           aTGGTTCATTAAATCTGATCGCCAGCGATGCATTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCgg                                                                                                 <  1:140294/149‑1 (MQ=255)
               ttCATTAAATCGGATCGCCAGCGATGCATTTTCAGGTAGTAACAATCTCGACCTGGCGTTAAAAAAAAGAAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGa                                                                                             <  1:230383/149‑1 (MQ=38)
                         cTGATCGCCAGCGATGCATTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatt                                                                                   >  2:12479/1‑149 (MQ=255)
                              cGCCAGCGATGCATTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGCCGGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcagttcatt                                                                              >  1:63158/1‑149 (MQ=255)
                              cGCCAGCGATGCATTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGGGCGAATAAGCGGGGGAATTCTTCTCGGCGGACTCAGtcatttcatt                                                                              >  1:204764/1‑149 (MQ=255)
                                ccAGCGATGCCTTTTCAGGTAGTAACGATCTCGACCTGGCGGTAGCCAGGGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGCGAAGGTGCGAATAACCGGGGAAATTCTTCTCGGCTGACTCAGTCatttcatttc                                                                            <  1:15207/149‑1 (MQ=37)
                                    cGATGCATTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATttctgcg                                                                        >  2:38329/1‑146 (MQ=255)
                                          aTTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTg                                                                  >  1:83668/1‑149 (MQ=255)
                                                   tagtaACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAttt                                                         >  2:46248/1‑149 (MQ=255)
                                                                    tGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGcc                                        >  1:38301/1‑149 (MQ=255)
                                                                              cAGCGGCAAAACTGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGc                              <  1:175862/149‑1 (MQ=255)
                                                                                   gCAAAAATGACAACCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTAtt                         >  1:190082/1‑149 (MQ=255)
                                                                                     aaaaaTGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTa                       >  2:275541/1‑149 (MQ=255)
                                                                                                          acaacagtagctgcAATTAGGGACTGTTATAATTAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTaa  <  2:225561/136‑1 (MQ=11)
                                                                                                                              |                                                                                                                                
ATTTGGCCCGAATGGTTCATTAAATCTGATCGCCAGCGATGCATTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAA  >  CP009273/3358789‑3359043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.