Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC CP009273 2,400,257 IS5 (+) +4 bp 50.0% intergenic (‑137/‑780) lrhA ← / → alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 = 2283592NA (NA)11 (0.620) 8/276 NT 54.3% noncoding (1/1195 nt) IS5 repeat region
?CP009273 = 2400260 9 (0.520)intergenic (‑140/‑780) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2
* ? CP009273 2400257 =9 (0.520)7 (0.410) 7/268 NT 43.8% intergenic (‑137/‑783) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2
?CP009273 3358915 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region

ACTGGCGGCTCATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGA  >  CP009273/2400133‑2400394
                                                                                                                               |                                                                                                                                      
tggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgaatcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTcc                                                                                                                   <  2:97115‑M1/25‑1 (MQ=255)
    gcggcTCATACTGAGCTGGTTAATGCTGTGCACACACAAACACGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggcgcgaacaagtccctgata                                                                                                               >  1:152407‑M2/1‑124 (MQ=255)
          cATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGTAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGc                                                                                                         <  2:153339/149‑1 (MQ=255)
                gAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAg                                                                                                   <  1:269544/149‑1 (MQ=255)
                               aaggcatttacgggagaaaaaatcggctcaaacgtgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAatat                                                                                    <  2:199364‑M1/56‑1 (MQ=255)
                                     acacaAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaac                                                                              >  2:132197‑M2/1‑91 (MQ=255)
                                             aaGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGAACGCAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCg                                                                      <  2:25307/149‑1 (MQ=255)
                                                cAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaccggttttgttga                                                                   >  1:53677‑M2/1‑80 (MQ=255)
                                                                 tgCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgggtcatcaacttccc                                                  <  1:311058‑M2/149‑87 (MQ=255)
                                                                       ccTGGCAGTTTCATCCGGAACCCGGCGGAAAGTAAAAATGCATATGAAAAAAAAAAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCttt                                            <  1:189643/149‑1 (MQ=255)
                                                                       ccTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacatggttcatcatgagtcatcaacttaccttcgcc                                            >  1:149234‑M2/1‑57 (MQ=255)
                                                                       ccTCGCCGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaacgtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgcc                                            >  1:149119‑M2/1‑57 (MQ=255)
                                                                                     ccGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcag                              >  2:283253‑M2/1‑43 (MQ=255)
                                                                                     ccGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcag                              >  2:8521‑M2/1‑43 (MQ=255)
                                                                                       cgaACCCGGACCAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccccgatatgagatcatgtttctcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagca                            >  2:12983‑M2/2‑41 (MQ=255)
                                                                                           cgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCgt                        <  2:265431‑M1/116‑1 (MQ=255)
                                                                                             agaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCgtgt                      >  1:97115‑M1/32‑149 (MQ=255)
                                                                                               aagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGttt                    >  2:309598‑M1/30‑149 (MQ=255)
                                                                                                   aaaGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtc                >  2:109087‑M2/1‑29 (MQ=255)
                                                                                                   aaaGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGtat                <  1:290942/149‑1 (MQ=255)
                                                                                                       tAAAAATGCATATGAGGTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaat            <  1:98979/149‑1 (MQ=255)
                                                                                                            aTGCATATGAGCTGCACTAAcgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaa       >  2:64090‑M2/1‑20 (MQ=255)
                                                                                                             ttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaatgtaaat      <  2:312531‑M1/134‑1 (MQ=255)
                                                                                                             atattcgcaccttgcCTAAAAAAGCGACTCACATGGTTCCGTTATAATGCCTTAAGGAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCTTGTTTGTAtacctgtaaat      >  1:253790‑M1/16‑149 (MQ=255)
                                                                                                                 tatGAGTTGCAGTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGa  <  2:2515/149‑1 (MQ=255)
                                                                                                                 tatGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGa  <  2:235391/149‑1 (MQ=255)
                                                                                                                 tatGAGGTGGACTAAAAAAGCGACTCACATTTTTCCTTTATAATGCCTTAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACGCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGa  <  2:126574/149‑1 (MQ=255)
                                                                                                                               |                                                                                                                                      
ACTGGCGGCTCATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGA  >  CP009273/2400133‑2400394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.