Notes

Publication:

10.1101/327270

10.1371/journal.pcbi.1006213

ALE, flask, and isolate counts

ALE replicate Flask count Isolate count
1 1 1
2 1 3
3 1 3
4 1 3
5 1 3
6 1 3
6 6 16

ALE experiment mutation counts

Mutation Type Unique Observed
Single Base Substitutions 49 110
Multiple Base Substitutions 1 1
Deletions 7 11
Insertions 4 12
Mobile Element Insertions 4 21
Amplifications 0 0
Gene Conversions 0 0
Inversions 0 0
Total 65 155

Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Unassigned Missing Coverage Evidence Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F26 I0 R1 19 0 19.3 701150 97.5% 683621 146.2
A2 F26 I0 R1 23 0 22.9 848682 95.4% 809642 144.9
A2 F26 I30 R1 7 0 85.8 2797110 96.5% 2699211 148.9
A2 F26 I40 R1 5 0 75.4 2442972 96.7% 2362353 148.8
A3 F21 I0 R1 26 0 23.4 828097 98.3% 814019 146.2
A3 F21 I30 R1 6 0 72.8 2370788 96.6% 2290181 149.0
A3 F21 I40 R1 12 0 60.0 2002308 97.3% 1948245 146.6
A4 F24 I0 R1 18 0 26.7 941114 95.9% 902528 148.9
A4 F24 I30 R1 7 0 75.6 2463364 96.2% 2369756 148.6
A4 F24 I40 R1 7 0 61.9 2001120 96.7% 1935083 148.9
A5 F33 I0 R1 24 0 36.4 1217072 98.1% 1193947 146.1
A5 F33 I30 R1 8 0 55.1 1786802 98.2% 1754639 146.6
A5 F33 I40 R1 9 0 118.4 3828688 98.0% 3752114 146.6
A6 F34 I0 R1 78 0 72.6 2478562 95.6% 2369505 148.9
A6 F34 I30 R1 8 0 101.8 3301630 96.8% 3195977 148.6
A6 F34 I40 R1 7 0 81.8 2653624 96.6% 2563400 148.7

Mutation Needle Plot

Mutation Targets

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