Notes

Publication:

10.1016/j.ymben.2018.06.005

ALE, flask, and isolate counts

ALE replicate Flask count Isolate count
0 1 1
9 1 3
10 1 3
11 1 3
12 1 3
5 5 13

ALE experiment mutation counts

Mutation Type Unique Observed
Single Base Substitutions 18 66
Multiple Base Substitutions 0 0
Deletions 10 75
Insertions 3 3
Mobile Element Insertions 0 0
Amplifications 0 0
Gene Conversions 0 0
Inversions 0 0
Total 31 144

Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Unassigned Missing Coverage Evidence Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 31 0 56.8 1065165 97.7% 1040666 253.4
A9 F131 I0 R1 80 0 103.3 1763998 98.4% 1735774 265.0
A9 F131 I1 R1 12 0 63.3 2199030 94.3% 2073685 142.9
A9 F131 I2 R1 13 0 45.3 1574262 94.4% 1486103 142.7
A10 F135 I0 R1 43 0 78.1 1390606 98.4% 1368356 267.4
A10 F135 I1 R1 8 0 67.1 2410154 94.6% 2280005 142.6
A10 F135 I2 R1 9 0 52.8 1916504 93.9% 1799597 141.5
A11 F138 I0 R1 39 0 66.8 1182276 98.7% 1166906 256.8
A11 F138 I1 R1 12 0 41.2 1437398 94.1% 1352591 142.5
A11 F138 I2 R1 10 0 46.7 1613674 94.9% 1531376 142.7
A12 F142 I0 R1 65 0 79.5 1442294 98.2% 1416332 263.9
A12 F142 I1 R1 11 0 59.1 2131346 94.5% 2014121 142.0
A12 F142 I2 R1 11 0 76.9 2733720 94.5% 2583365 142.8

Mutation Needle Plot

Mutation Targets

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