Notes

Publication:

10.1128/AEM.00410-17

ALE, flask, and isolate counts

ALE replicate Flask count Isolate count
0 1 1
7 1 12
8 1 13
9 1 10
4 4 36

ALE experiment mutation counts

Mutation Type Unique Observed
Single Base Substitutions 48 217
Multiple Base Substitutions 0 0
Deletions 25 136
Insertions 2 8
Mobile Element Insertions 8 39
Amplifications 0 0
Gene Conversions 0 0
Inversions 0 0
Total 83 400

Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Unassigned Missing Coverage Evidence Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4 1 422.4 11956043 98.9% 11824526 164.0
A7 F90 I0 R1 54 20 129.4 2031886 96.4% 1958738 285.8
A7 F90 I0 R2 30 8 112.2 1976983 88.9% 1757537 285.2
A7 F90 I0 R3 40 8 146.9 2297919 97.7% 2245066 286.4
A7 F90 I1 R1 13 4 64.3 1373750 72.9% 1001463 300.4
A7 F90 I2 R1 13 3 65.6 1278727 80.0% 1022981 300.4
A7 F90 I3 R1 11 2 267.5 5183891 88.6% 4592927 276.0
A7 F90 I4 R1 13 4 51.2 939505 89.5% 840856 286.9
A7 F90 I5 R1 24 3 61.2 1135137 88.5% 1004596 286.9
A7 F90 I6 R1 16 5 85.2 1642287 81.9% 1345033 300.6
A7 F90 I7 R1 19 5 83.6 1539361 84.6% 1302299 300.6
A7 F90 I8 R1 12 4 70.9 1364818 81.4% 1110961 300.6
A7 F90 I9 R1 15 4 182.4 3327383 85.7% 2851567 300.6
A8 F91 I0 R1 29 28 124.7 1952491 96.8% 1890011 285.3
A8 F91 I0 R2 42 3 81.4 1505765 88.3% 1329590 282.1
A8 F91 I1 R1 11 3 79.7 1483581 89.0% 1320387 285.2
A8 F91 I2 R1 11 4 74.1 1377522 89.7% 1235637 286.6
A8 F91 I3 R1 11 5 97.5 1793558 89.6% 1607027 287.0
A8 F91 I4 R1 11 3 122.9 2233892 89.9% 2008268 288.6
A8 F91 I5 R1 15 5 132.2 2398682 89.8% 2154016 288.1
A8 F91 I6 R1 15 4 129.6 2330251 89.9% 2094895 290.6
A8 F91 I7 R1 13 3 139.5 2546366 90.0% 2291729 287.1
A8 F91 I8 R1 9 4 81.3 1714908 73.9% 1267317 300.6
A8 F91 I9 R1 11 4 67.7 1668216 63.4% 1057648 300.6
A8 F91 I10 R1 11 4 70.1 1546763 70.6% 1092014 300.6
A8 F91 I11 R1 11 4 98.1 2135868 72.1% 1539960 300.6
A9 F92 I0 R1 51 39 146.1 2193620 97.0% 2127811 289.7
A9 F92 I0 R2 37 3 124.6 2246525 89.6% 2012886 283.8
A9 F92 I1 R1 12 2 129.3 2355075 89.0% 2096016 291.1
A9 F92 I2 R1 14 2 170.8 3126081 90.1% 2816598 287.4
A9 F92 I3 R1 14 2 218.9 3952508 89.8% 3549352 290.5
A9 F92 I4 R1 16 3 228.5 4127912 90.0% 3715120 290.6
A9 F92 I5 R1 15 2 73.5 1537466 74.9% 1151562 300.6
A9 F92 I6 R1 18 5 65.3 1472746 69.2% 1019140 300.6
A9 F92 I7 R1 17 4 117.3 2470152 74.3% 1835322 300.6
A9 F92 I8 R1 15 3 104.2 2166912 75.2% 1629517 300.6

Mutation Needle Plot

Mutation Targets

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