Ecoli_SugarSynth_CO2: 2_chemostat - Sample Meta Data
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Note: the reference genome BOP27 is a known variant of E. coli K12 MG1655 E. coli K12 MG1655.
| ALE, Flask, Isolate, Technical Replicate | Clonal or Population | ALE Sample Details | Base Media | Carbon Source(g/L) | Nitrogen Source(g/L) | Phosphorus Source(g/L) | Sulfur Source(g/L) | Calcium Source(g/L) | Supplement(g/L) | Temperature (Celsius) | Strain | Additional Strain Details | Taxonomy ID | Reference Genome |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A0 F0 I1 R1 | clonal | M9 | NH4Cl(1) | KH2PO4(3),Na2HPO4(6.8) | MgSO4(0.24) | CaCl2(0.1) | 37 | 679895 | E. coli BW25113 delta-gpmA,gpmM,pfkA,pfkB,zwf,aceBAK pCBB | ['/var/data/KX077536.1.gb', '/var/data/CP009273.1.gb'] | ||||
| A1 F1 I1 R1 | clonal | xylose-limiting condition; To avoid the possibility that pyruvate becomes the limiting nutrient instead of xylose, in the second and fourth experiments, xylose concentrations were lowered in the feed media whenever a significant drop in residual pyruvate was detected in the culture | M9 | sodium pyruvate(5 g/L),xylose(25 mg/L),carbon dioxide(25%) | NH4Cl(1) | KH2PO4(3),Na2HPO4(6.8) | MgSO4(0.24) | CaCl2(0.1) | chloramphenicol(34 mg/L), | 37 | 679895 | E. coli BW25113 delta-gpmA,gpmM,pfkA,pfkB,zwf,aceBAK pCBB; ancestral strain inoculated contained a ∆mutS mutation | ['/var/data/KX077536.1.gb', '/var/data/CP009273.1.gb'] |