Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 242,857 G→A G829D (GGT→GAT)  JCVSYN2_01035 → ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816242,8570GA99.9% 5304.1 / NA 1640G829D (GGT→GAT) JCVSYN2_01035ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (784/853);  minor base T (0/2);  total (785/855)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1640 total aligned reads displayed.

AAGAACTTGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGGTTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACGATTAAT  >  CP016816/242732‑242981
                                                                                                                             |                                                                                                                            
aaGAACTTGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGAtt                                                                                                                            >  2:3177081/1‑128 (MQ=255)
aaGAACTTGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGAtt                                                                                                                            >  2:1011420/1‑128 (MQ=255)
     cTTGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCa                                                                                                                       <  1:262326/128‑1 (MQ=255)
        gATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAAtt                                                                                                                    >  1:2720504/1‑128 (MQ=255)
         aTTTTAAAAAGCTAAGTTTACTAATAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAAttg                                                                                                                   <  2:3843981/128‑2 (MQ=255)
         aTTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTc                                                                                                                   >  1:3660686/1‑128 (MQ=255)
          tttAAAAAAGCTAAGTTTACTACTTAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTcc                                                                                                                  <  2:921769/128‑1 (MQ=255)
              aaaaaGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAAttgatgga                                                                                                              <  2:1648611/128‑7 (MQ=255)
               aaaaGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCaa                                                                                                             >  2:1545156/1‑128 (MQ=255)
               aaaaGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCaa                                                                                                             >  2:826268/1‑128 (MQ=255)
                aaaGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAAt                                                                                                            <  1:1001960/128‑1 (MQ=255)
                aaaGATAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAAt                                                                                                            <  1:2364553/128‑1 (MQ=255)
                 taGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAAtt                                                                                                           <  2:2676644/127‑1 (MQ=255)
                 aaGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAAtt                                                                                                           <  1:1989566/128‑1 (MQ=255)
                   gCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGc                                                                                                         <  2:2834068/128‑1 (MQ=255)
                    cTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCt                                                                                                        <  1:896445/128‑1 (MQ=255)
                     tAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCtt                                                                                                       <  1:1601417/128‑1 (MQ=255)
                       aGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCtttt                                                                                                     <  2:3448307/128‑1 (MQ=255)
                        gTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTa                                                                                                    >  2:1420288/1‑128 (MQ=255)
                         tttACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTAc                                                                                                   >  2:769470/1‑128 (MQ=255)
                          ttACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCATATTTATCAATTCAATCAATTGATTTTACt                                                                                                  >  1:2816669/1‑128 (MQ=255)
                          ttACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACt                                                                                                  >  2:359562/1‑128 (MQ=255)
                           tactacTTAATTTAGTTTAACTTTAGCTTCTTCTGGTTAAAAAAATATCTTTTTATTAGTACCTGTTGTTGGAATTTCAACTAAGTATATTACATCAGATATATCAATTCCATCAAATGCTTTTAcaa                                                                                                 >  2:1986456/1‑126 (MQ=255)
                             ctactaAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTAtt                                                                                               >  2:3762339/1‑128 (MQ=255)
                              tactaAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTa                                                                                              >  1:3740892/1‑128 (MQ=255)
                              tactaAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTa                                                                                              >  2:240961/1‑128 (MQ=255)
                               actaAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTaa                                                                                             <  1:382298/128‑1 (MQ=255)
                                 taAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTaaaa                                                                                           <  2:2242656/128‑1 (MQ=255)
                                  aaaTTTATGTTAACTTTAGCTGCTTCTGGTAAAAAATATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTaaaaa                                                                                          <  2:2586789/128‑1 (MQ=255)
                                   aaTTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAac                                                                                         <  1:3198333/128‑1 (MQ=255)
                                         gTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACttat                                                                                   <  2:2485706/128‑1 (MQ=255)
                                          tttAACTTTAGCTTCTTCTGGTAAAAAATATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACttatt                                                                                  <  1:58324/128‑1 (MQ=255)
                                          gttAACTTTATCTTCATAAGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACttatt                                                                                  <  1:175954/127‑1 (MQ=255)
                                            tAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTtattat                                                                                >  1:959049/1‑128 (MQ=255)
                                             aaCTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATtata                                                                               <  2:562263/128‑1 (MQ=255)
                                               cTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATaaa                                                                             >  1:2180356/1‑128 (MQ=255)
                                               cTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATaaa                                                                             >  2:3690503/1‑128 (MQ=255)
                                                tttAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTTTTGTTGGAATTTCAACTATGTTTATTTCATCAGATTTATCAATTCAATCAATTTCTTTTAATATTAAAAACACTTATTATaaaa                                                                            >  2:2939249/1‑128 (MQ=255)
                                                tttAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTg                                                                                                          >  1:2255677/1‑98 (MQ=255)
                                                tttAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATaaaa                                                                            <  1:2306566/128‑1 (MQ=255)
                                                tttAGCTTATTCTTGTAAATAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATTTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATaaaa                                                                            <  2:1111048/128‑1 (MQ=255)
                                                   aGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTa                                                                         <  1:3310306/128‑1 (MQ=255)
                                                   aGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTa                                                                         <  1:1132827/128‑1 (MQ=255)
                                                      ttcttcTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATATCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAATAGTATTa                                                                      <  1:3602977/128‑1 (MQ=255)
                                                        cttctGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAAtt                                                                                                                    >  1:1493744/1‑80 (MQ=255)
                                                         ttctGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAat                                                                   <  1:1900452/128‑1 (MQ=255)
                                                          tctGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAata                                                                  <  1:1197105/128‑1 (MQ=255)
                                                           ctGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAatat                                                                 <  2:2767347/128‑1 (MQ=255)
                                                            tGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATg                                                                <  2:3100090/128‑1 (MQ=255)
                                                                aaaaaaaaTATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCaaa                                                            <  1:3892415/128‑1 (MQ=255)
                                                                  aaaaaaTATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATg                                                          <  1:1255297/128‑1 (MQ=255)
                                                                     aaaTATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAat                                                       <  2:405057/128‑1 (MQ=255)
                                                                        tatCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAAtata                                                    >  2:2354050/1‑128 (MQ=255)
                                                                        tatCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCAATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAAtata                                                    <  1:2088862/128‑1 (MQ=255)
                                                                          tCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAat                                                  >  2:1889975/1‑128 (MQ=255)
                                                                          tCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAat                                                  >  1:297450/1‑128 (MQ=255)
                                                                            tttttATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAatta                                                <  2:3216940/128‑1 (MQ=255)
                                                                             ttttATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAattat                                               >  1:523274/1‑128 (MQ=255)
                                                                              tttATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAattatt                                              <  2:3944664/128‑1 (MQ=255)
                                                                               ttattaGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAattatac                                             >  2:120370/1‑126 (MQ=255)
                                                                               ttattaGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAAATCCATCAATTGCTTTTACTATTAAAAAAACTTAATATAAAAGTAATAAATATGCAAATGAAAATAAATATTc                                             >  2:1347546/1‑128 (MQ=255)
                                                                                 attaGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCaa                                           >  1:1055208/1‑128 (MQ=255)
                                                                                  ttaGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCaaa                                          <  2:3374593/128‑1 (MQ=255)
                                                                                  ttaGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCaaa                                          >  2:3978999/1‑128 (MQ=255)
                                                                                    aGTAACTGTTGTTGGAATTTCAACTATGTTTAATTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAg                                        <  2:630624/128‑1 (MQ=255)
                                                                                     gTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCaaaatt                                       >  2:2169220/1‑126 (MQ=255)
                                                                                     gTAACTCGTGTTGGAATTGCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCATTTGATTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAgg                                       <  2:1277735/128‑1 (MQ=255)
                                                                                      tAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGATTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGgt                                      <  1:3972079/128‑1 (MQ=255)
                                                                                       aaCTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGgtg                                     >  1:645517/1‑128 (MQ=255)
                                                                                        aCTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGgtgt                                    <  1:2911617/128‑1 (MQ=255)
                                                                                         cTGTTGTTTGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGtt                                   <  2:229412/128‑1 (MQ=255)
                                                                                         cTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGtt                                   <  1:454986/128‑1 (MQ=255)
                                                                                         cTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGtt                                   <  2:1489117/128‑1 (MQ=255)
                                                                                         cTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGtt                                   >  1:3135396/1‑128 (MQ=255)
                                                                                           gttgttGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTc                                 >  1:1838501/1‑128 (MQ=255)
                                                                                                tgGAATTTAAACTATTATAATTTCATAAGATTTATCAATTTCATAAATTTCTTTTACTAATAAAAACAATTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAAtt                            <  1:2566355/128‑1 (MQ=255)
                                                                                                  gAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTACAAATtct                          >  1:3048415/1‑128 (MQ=255)
                                                                                                   aaTTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATtctc                         >  1:2123437/1‑128 (MQ=255)
                                                                                                   aaTTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATtctc                         >  2:1813541/1‑128 (MQ=255)
                                                                                                    aTTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTcc                        <  1:1390769/128‑1 (MQ=255)
                                                                                                    aTTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGCTGTTTCAAATTCTcc                        >  1:719847/1‑128 (MQ=255)
                                                                                                     tttCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCt                       >  2:1742085/1‑128 (MQ=255)
                                                                                                        cAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGgtgt                                    <  1:778044/112‑1 (MQ=255)
                                                                                                        cAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTa                    >  1:1711064/1‑128 (MQ=255)
                                                                                                        cAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTa                    >  2:512010/1‑128 (MQ=255)
                                                                                                         aaCTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTaa                   <  1:1327638/128‑1 (MQ=255)
                                                                                                          aCTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAAc                  >  1:819184/1‑128 (MQ=255)
                                                                                                             aTGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACaaa               >  1:2456563/1‑128 (MQ=255)
                                                                                                             aTGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACaaa               >  1:4019989/1‑128 (MQ=255)
                                                                                                             aTGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACCTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACaaa               >  2:963262/1‑128 (MQ=255)
                                                                                                               gTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAacac             >  2:3006404/1‑128 (MQ=255)
                                                                                                               gTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAAc             >  2:352273/1‑128 (MQ=255)
                                                                                                               gTTTATTTCAGCAGATTTAGCAAATACATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTTAAATTCTCCTTTAACAAAAc             <  1:3374694/128‑1 (MQ=255)
                                                                                                                   aTTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAAc         <  1:3537267/128‑1 (MQ=255)
                                                                                                                    tttCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACg        <  2:3287769/128‑1 (MQ=255)
                                                                                                                     ttCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACGa       <  2:723768/128‑1 (MQ=255)
                                                                                                                       cagcagATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACGatt     <  2:465551/128‑1 (MQ=255)
                                                                                                                       cagcagATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACGatt     >  1:3255387/1‑128 (MQ=255)
                                                                                                                         gcagATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAACCCAACGattaa   >  2:2100086/1‑128 (MQ=255)
                                                                                                                          cagATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGTTGTTTCAAATTCTCCTTTAACAAAACCAAATattaat  >  1:34389/1‑128 (MQ=255)
                                                                                                                             |                                                                                                                            
AAGAACTTGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGGTTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACGATTAAT  >  CP016816/242732‑242981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: