BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	165,218	A→T	intergenic (+182/‑360)	JCVSYN2_00665 → / → JCVSYN2_00670	tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	165,218	0	A	T	98.1%	4036.3 / ‑1.3	1365	intergenic (+182/‑360)	JCVSYN2_00665/JCVSYN2_00670	tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand): ref base A (4/22); new base T (807/531); total (812/553)
Fisher's exact test for biased strand distribution p-value = 5.25e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1415 total aligned reads displayed.

TTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGT  >  CP016816/165093‑165346

   aCTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTtaaaaa                                                                                                                             <  2:2781036/128‑3 (MQ=255)

    atttAATTGTATTAATTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTaaaaaa                                                                                                                            <  2:3591581/127‑1 (MQ=255)

     tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAaat                                                                                                                           >  1:1921294/1‑128 (MQ=255)

     tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAaat                                                                                                                           >  1:1670973/1‑128 (MQ=255)

       tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAaatta                                                                                                                         <  1:1008035/128‑1 (MQ=255)

        aaTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAaattaa                                                                                                                        <  1:942656/128‑1 (MQ=255)

        aaTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAaattaa                                                                                                                        >  1:363030/1‑128 (MQ=255)

            gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTg                                                                                                                    >  2:3461948/1‑128 (MQ=255)

             tATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGa                                                                                                                   <  1:3152680/128‑1 (MQ=255)

              aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGaa                                                                                                                  >  2:1159728/1‑128 (MQ=255)

              aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGaa                                                                                                                  >  1:801564/1‑128 (MQ=255)

              aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGaa                                                                                                                  <  1:3101562/128‑1 (MQ=255)

              aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGaa                                                                                                                  <  1:1546577/128‑1 (MQ=255)

               ttactttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAAc                                                                                                                 >  1:2135210/1‑128 (MQ=255)

                tactttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACt                                                                                                                <  2:1312015/128‑1 (MQ=255)

                  ctttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTg                                                                                                              <  2:2955898/128‑1 (MQ=255)

                    ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATAATTTTTTTATATGCAAA‑TTTATAAAAAATTAATTTGAACTTGTa                                                                                                            >  1:874177/1‑128 (MQ=255)

                            tGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATat                                                                                                    >  1:536689/1‑127 (MQ=255)

                             gATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGc                                                                                                   >  1:1291136/1‑128 (MQ=255)

                             gATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGc                                                                                                   >  1:3469730/1‑128 (MQ=255)

                                aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCttt                                                                                                >  1:2213701/1‑128 (MQ=255)

                                aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttt                                                                                                >  1:2670828/1‑128 (MQ=255)

                                 atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttt                                                                                               >  2:866652/1‑128 (MQ=255)

                                    tttAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttt                                                                                            >  2:2380036/1‑128 (MQ=255)

                                        gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttt                                                                                        >  2:286094/1‑128 (MQ=255)

                                        gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttattttt                                                                                        >  2:560927/1‑128 (MQ=255)

                                         tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCtttttttttttt                                                                                       >  1:602515/1‑128 (MQ=255)

                                           tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttt                                                                                     >  2:3768986/1‑128 (MQ=255)

                                              ttAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGCtttttttttttttttac                                                                                  <  1:3026097/128‑3 (MQ=255)

                                              ttAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttt                                                                                  >  2:1518664/1‑128 (MQ=255)

                                                  tttATTGCACTTGTATGAAATAGTAACAAACAAAAAATATTATCTTTTTAGATATTTTTTGTATATTCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAAAAGCTTTTTTTTTTTTTTTTtataa                                                                              <  1:2592713/128‑2 (MQ=255)

                                                  tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTtataa                                                                              >  1:751350/1‑127 (MQ=255)

                                                    tatatcACTTTTATGAATTATTAATAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTTTAAAAAATAGCTTTTTTTTTTTTTTTTtataatg                                                                            <  1:2272246/123‑4 (MQ=255)

                                                     ttCGCATTTTTATGAATTATTAATAAAATAAAAATTTTTTTTTTTTTTATATTTTTTTTTTATTCAAA‑TTTTTAAAAAATTAATTTTAACTTTTAAAAAATATCTTTTTTTTTTTTTTTTtataatgg                                                                           <  2:3506072/127‑5 (MQ=255)

                                                       cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttataatttaaa                                                                         >  1:468546/1‑118 (MQ=255)

                                                         cACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttaaatgtaaat                                                                       >  2:1765067/1‑118 (MQ=255)

                                                            attatttaaTTTGTAATAAACTTAAAATATTATATTTTTATTTATTTTTTGTTTATTCAAA‑TTTTTAAAAAATTAATTTTTACTTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAAtata                                                                  <  2:2286812/121‑1 (MQ=255)

                                                               ttttaaTTATTAAGAAAAAAAAAATATTATCTTTTTATATAATTTTTTTATATGCAAA‑TTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAAt                                                               <  2:3055160/124‑1 (MQ=255)

                                                               tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATATTGTAAATATAAAt                                                               >  2:1012859/1‑128 (MQ=255)

                                                                 tGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAAAATAAAttt                                                             >  1:3937463/1‑128 (MQ=255)

                                                                   aaattctAAGTAACTAAAAATAATATATTTTTATATATTTTTTGTTAATCCAAA‑TTTTTAAAAAATTAATTGTAACTTGTAAAAAAAACC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTa                                                           <  2:1991413/122‑1 (MQ=255)

                                                                    atttttaataaAATAAAAATATTATATTTTTTGTTTTTTTTTTTAAATTAAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAg                                                          <  2:3409215/123‑1 (MQ=255)

                                                                      tagtaatatcataaaacttTTATATTTTTTTATATTTTTTGTATAAGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                        <  2:2074035/117‑1 (MQ=255)

                                                                      tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGaa                                                                                                                  <  1:1402728/72‑1 (MQ=255)

                                                                        tttataaactaaaaattTTTTATTTTTATTTATTTTTTTTATATGCAAA‑TTTTTAAAAAAATAATTTTTACTTGTAAAAAATACC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAt                                                      <  1:3518611/123‑1 (MQ=255)

                                                                        gTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTGAAATATAAATTTtatttatt                                                     >  1:3359421/1‑128 (MQ=255)

                                                                        gTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAAATTTTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATATTGAAAATATTATTTTTattta                                                       >  1:3996528/1‑124 (MQ=255)

                                                                         taataaAATAAAAATATTTTTTTTTTATTTTTTTTTTGTAATTTTAAA‑TTTTTAAAAAATTAATTTTAAATTTTAAAAAATAG‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAtt                                                     <  1:3576810/128‑1 (MQ=255)

                                                                          aaGAAACTAAAACTATTATATTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTCTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTAAAATGTAAATATTAATTTtatttattat                                                   >  2:1904180/1‑126 (MQ=255)

                                                                             aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATttttttt                                               >  1:2085664/1‑127 (MQ=255)

                                                                             aaaataaaaattTTTTATTTTTTTATTTTTTTTTTTTATTTAAA‑TTTTTAAAAAATTTATTTTAAATTTTATAAAAT‑TTATTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttg                                                  <  1:654049/128‑1 (MQ=255)

                                                                              taataaaaataTTATTTTTTTTTTATTTTTTTTTATATTTAAATTTTTAAAAAAATAAATTTTAA‑TTTTATAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttgtt                                                <  1:693005/125‑1 (MQ=255)

                                                                              aactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTATTTTTTTTTTTAAAATTTAAATATAAATTTTAGttttttttttt                                               >  2:2123505/1‑127 (MQ=255)

                                                                                ataaaaattTTATATTTTTTTATTTTTTTTTTATATTAAAA‑TTTTTAAAAAATTAATTTTTAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttgtt                                                <  2:1854155/127‑1 (MQ=255)

                                                                                ataaaaataTTATTTTTTTTTATATTTTTTTTTTATTAAAA‑TTTTTAAAAAATTTATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttgttg                                               <  2:4023946/127‑1 (MQ=255)

                                                                                 taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAATTATAAATTTTATTTATtttttttaa                                             >  1:1053436/1‑125 (MQ=255)

                                                                                 taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATAAAATTTTTAGTTATttttttttaa                                            >  2:927433/1‑124 (MQ=255)

                                                                                 taaaaataTTATTATTTTTTTTAATTTTTTTATATTTAAA‑TTTTTTAAAAAATAATTTTAAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTc                                             <  1:291544/128‑1 (MQ=255)

                                                                                  aaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAAAGTAAATATAAATTTAATTAATTTTTTTTTCTa                                           >  1:411046/1‑128 (MQ=255)

                                                                                  aaaaataTTATATTTTTATATATTTTTTTTATATTTAAA‑TTTTTAAAAAATTAATTTTAACTTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCt                                            <  1:1450356/128‑1 (MQ=255)

                                                                                     agatttatATTTTTATTTATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTTAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                         <  1:3790435/124‑1 (MQ=255)

                                                                                     aatattatCTTTTTATATATTTTTTTTATATTCAAA‑TTTTTAAAAAATTAATTTTAAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                         <  2:1441409/126‑1 (MQ=255)

                                                                                      ctaTTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATGGAAATA‑AAATTTTATGTttttttttttaaatctt                                       >  1:2006344/1‑119 (MQ=255)

                                                                                      ctaTTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGT‑AAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATTTAAATATAAATTTAAGTTATTTTTTTTTATAATTa                                       >  2:691521/1‑128 (MQ=255)

                                                                                      ctaTTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTATAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATTATGAAAAAATAATTTTTATATTTTtttttgaataa                                          >  2:1650800/1‑123 (MQ=255)

                                                                                       tattatCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAAAAT‑AAAATTAAAATTTTAAGTATtttttttttaatat                                        >  2:460371/1‑120 (MQ=255)

                                                                                       tattatCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTATttttttttatattta                                       >  1:1250719/1‑119 (MQ=255)

                                                                                       tattatATTTTTTTATATTTTTTGTAAATTCAAA‑TTTTTAAAAAATTAATTTTAAATTTTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATc                                     <  2:3684429/128‑1 (MQ=255)

                                                                                        attatCTTTTTAGATATTTTTTGTATTATAAAA‑TTTTTAAAAAATTAATTTGAACTTTTAAAAATTA‑‑TGTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTAt                                      <  1:1763139/128‑1 (MQ=255)

                                                                                         ttatCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTATTTTTTTGTATAATTAta                                     >  1:2796225/1‑127 (MQ=255)

                                                                                          tttttttttATTTATTTTTTTTATATTTAAA‑TTTTTTAAAAATTTATTTTTAATTTTAAAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCt                                    <  1:1589530/124‑1 (MQ=255)

                                                                                          tatCTTTTTTTTTATTTTTTTTATATTATAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCt                                    <  1:1145793/128‑1 (MQ=255)

                                                                                           atCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTTTGTATTTTGGTGTCTAATTATCTa                                   >  2:2714898/1‑128 (MQ=255)

                                                                                              tttttAGATATTTTTTGTATATTCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTCTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaa                               <  1:2415790/128‑1 (MQ=255)

                                                                                                tttAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTATTTTGTTTTATAATTATATaaaaaaa                             >  1:3279309/1‑128 (MQ=255)

                                                                                                 ttAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATGTTTTTTGTTGTATAAGTATCTaaaaaaa                             >  2:609198/1‑128 (MQ=255)

                                                                                                 ttAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATA‑‑‑‑‑TCTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                          <  1:133154/128‑1 (MQ=255)

                                                                                                  tAGATATATTTTTTATATTTAAA‑TTTTTAAAAAATTAATTTTAAATTGTAAAAAATAG‑‑‑ATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATa                           <  1:270607/128‑1 (MQ=255)

                                                                                                   ttttatTTTTTGTATATTAAAA‑TTTTTAAAAAATTAAATTTAACTTGTTAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                          <  1:3698915/125‑1 (MQ=255)

                                                                                                   atatatTTTTTGTATATGAAAA‑TTTTTAAAAAATTAATTTGTAATTGTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                          <  2:3252822/126‑1 (MQ=255)

                                                                                                   aGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGAAAATATAAATTTTAGTTATTTTTTTTTATAATTATATAAAAAATTAg                          >  1:2319456/1‑128 (MQ=255)

                                                                                                    tttatTTTTTTTTTTTTGAAA‑TTTTTTAAAAATTAATTTTTAATTGTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                         <  1:1835104/126‑1 (MQ=255)

                                                                                                    gATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTAAGTTATTTTTTTGTCTAATTATCTAAAAAAATaaa                         >  1:3422750/1‑126 (MQ=255)

                                                                                                     atatTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTTAAAAAAAAATTATAGATATTTTTTTGTTTAAGTATATAAAAAAATATaa                        >  2:2583640/1‑128 (MQ=255)

                                                                                                       atTTTTTTTATATTCAAA‑TTTTTAAAAAATTAATTTTTAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGaaa                       <  2:1378374/128‑1 (MQ=255)

                                                                                                       atTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGTTATTTTTTTGTAAAATTATAAAAAAAAATATaaa                       >  2:776089/1‑128 (MQ=255)

                                                                                                         tttttGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTAAAAAAAAAATTTAATTTATTTTTTTTTCTAATAATAAAAAAAAATATaaatt                     >  1:2853060/1‑126 (MQ=255)

                                                                                                          ttttGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTATTTTGTTGTATAATTATCTAAAAAAATATAAAGTaa                   >  2:2222182/1‑128 (MQ=255)

                                                                                                           tttGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTATTTTGTTTTAAAATTATATAAAAAAATATaattaaa                   >  2:3925638/1‑123 (MQ=255)

                                                                                                            ttGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTT‑TAATGTAAAAATAAATTTTATTTATTTTTTTTTATAATTATATAaaaaaaaaaaaaataa                   >  2:1253457/1‑124 (MQ=255)

                                                                                                             tgtttttttaaa‑TTTTTAAAAAATTATATTGTAATTGTTAAAAA‑A‑TTGTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaa                 <  2:3137705/119‑1 (MQ=255)

                                                                                                             tgtttttGCAAA‑TTTTTAAAAAATTTATTTTTAATTTTTAAAAAT‑TTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                  <  2:3203045/122‑1 (MQ=255)

                                                                                                             tGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATATAAAAAAATAGAAAGGaataa                >  1:2477976/1‑128 (MQ=255)

                                                                                                              ttataTTTAAA‑TTTTTTAAAAATTAATTTTAAATTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaaaa               <  1:3860956/127‑1 (MQ=255)

                                                                                                                atttgaaaa‑TTTTTAAAAAATTTATTTGAAGTTTTAAAAAA‑‑‑‑TTGTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAatat            <  2:2499724/122‑1 (MQ=255)

                                                                                                                atatGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATAAAAATTTTATGTATTTTTTTTTATAAGTATATAAAAAAATAAAAAGTAAAAAat              >  2:3343672/1‑128 (MQ=255)

                                                                                                                      aaa‑TTTTTTAAAAAATAATTTTAAATTTTAAAAAATA‑‑‑TGTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTa       <  2:3869320/128‑1 (MQ=255)

                                                                                                                       aa‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTATAAGTATATAAAAAAATAGAAAGTAAAAAATATTTTTaa      >  2:107971/1‑128 (MQ=255)

                                                                                                                        a‑TTTTTTAAAAATTTATTTGAAAATTTAAAAAATA‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTaaa     <  2:1584550/128‑1 (MQ=255)

                                                                                                                           tttAAAAAAATTAATTT‑AACATTTAAAAAA‑A‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGt  <  1:3207880/128‑1 (MQ=255)

TTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGT  >  CP016816/165093‑165346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`