Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816437,8802.T46.7% inf / inf 585intergenic (‑184/+238)JCVSYN2_01985/JCVSYN2_01990glycosyl transferase/ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base . (140/172);  new base T (161/112);  total (301/284)
Fisher's exact test for biased strand distribution p-value = 6.88e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.09e-10

Only 100 of 934 total aligned reads displayed.

TGAAACTTATTTTTGTAGTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑ATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTTTTATTTATAGTTTATAAAGTTAATAATTTAGATTTTTAA  >  CP016816/437753‑438007
                                                                                                                                     |                                                                                                                                     
tGAAACTTATTTTTGTAGTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑g                                                                                                                                         >  2:3924486/1‑128 (MQ=255)
 gAAACTTATTTTTGTAGTTGTAAGTTTCATTTTTAATAAATATTAGAATTAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGATGCAAAGCAAATAACTCCATATTACGTATAGAGTTAAT‑‑‑‑G‑‑‑‑a                                                                                                                                    >  1:1637351/1‑128 (MQ=255)
 gAAACTTATTTTTGTAGTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑a                                                                                                                                    >  1:2796105/1‑128 (MQ=255)
  aaaCTTATTTTTGTAGTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑at                                                                                                                                   >  1:3313549/1‑128 (MQ=255)
       tATTTTTGTAGTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑atatata                                                                                                                              >  2:3393057/1‑128 (MQ=255)
        tttttaGTAGTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGATATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑agataaaa                                                                                                                             >  2:3717032/2‑125 (MQ=255)
        aTTTTTGTAGTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑atatatat                                                                                                                             >  2:2006194/1‑128 (MQ=255)
        aTTTTTGTAGTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑atatatat                                                                                                                             >  1:1881280/1‑128 (MQ=255)
         caactacaaaaatAAGTTTCATTTTTAATAAATATTAGATATAAAAATTGTATTTTTAAAATAAATATATTAGGAAATGAAGCAAAGCAAAAACCTCAATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑atatttata                                                                                                                            >  2:1715087/13‑128 (MQ=255)
           tttGTAGTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGTAAATGAAGCAAAGCAAAAACCTCCATATTAACTATAGAGTTAAT‑‑‑‑G‑‑‑‑atatatatata                                                                                                                          >  1:2099008/1‑128 (MQ=255)
           tttGTAGTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑atatatatata                                                                                                                          >  1:979395/1‑128 (MQ=255)
           tttGTAGTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑atatatatata                                                                                                                          >  2:1927547/1‑128 (MQ=255)
            ctacaaaaatAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑ataaaaatatat                                                                                                                         >  2:1387114/10‑128 (MQ=255)
              acaaaaatAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑atatatatatatat                                                                                                                       >  2:2397815/8‑128 (MQ=255)
                aGTTGTAATTTTAATTTTTAATAAATATTATAAATAAAAATTGTATTTTTAAAATAAATATTTTATGAAATTAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑atatatatatatatat                                                                                                                     <  2:3085584/128‑1 (MQ=255)
                aGTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑atatatatatatatat                                                                                                                     >  2:2129568/1‑128 (MQ=255)
                 gTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑atatatatatatatata                                                                                                                    >  2:2819043/1‑128 (MQ=255)
                  tttttaGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGTAAATTAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑atatatatatatatatat                                                                                                                   <  1:452023/123‑1 (MQ=255)
                    gTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTATGAAATGAATCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑atatatatatatatatatat                                                                                                                 <  1:2240855/128‑1 (MQ=255)
                    gTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑T‑‑‑‑atatatatatatatatatat                                                                                                                 >  2:1110806/1‑128 (MQ=255)
                    gTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑atatatatatatatatatat                                                                                                                 >  1:1000692/1‑128 (MQ=255)
                    gTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAACCAAACCAAAAACCACCATATAACCTATAGAGATAAT‑‑‑‑G‑‑‑‑atatatatatatatatatat                                                                                                                 <  1:1709968/128‑1 (MQ=255)
                     tatgTTTCATTTTTTATATATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑atatatatatatatatatata                                                                                                                <  2:4027668/125‑1 (MQ=255)
                      aaGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑atatatatatatatatatatat                                                                                                               >  2:534426/1‑128 (MQ=255)
                       aGTTTAATTTTTAATAAATATTATATATAAAAATTTTATTTTTAAAATAAATATTTTATTAAATGAATCAAATAAAAAACCTCAATATTAAATATAGAGTTAAT‑‑‑‑t‑‑‑‑atatatatatatatatatatata                                                                                                              <  2:881556/128‑1 (MQ=255)
                        ttttAATTTTTAATAAATATTTGAAATAAAAATTGTATTTTTATAATAAATATTTTATGAAATGAAGAAAATCAAAAAACTCCATATTATCTATAGAGTTAAT‑‑‑‑T‑‑‑‑atatatatatatatatatatatat                                                                                                             <  2:3661305/127‑1 (MQ=255)
                        gTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑atatatatatatatatatatatat                                                                                                             >  1:508313/1‑128 (MQ=255)
                          tttttttttAATAAATATTATAAATAAAAATTTTATTTATAAAATAAATATTTTTTTAAATGTAGCAAATCCAAAACCTCAATATAACATATAGAGATAAT‑‑‑‑T‑‑‑‑atatatatatatatatatatatatat                                                                                                           <  2:852741/124‑1 (MQ=255)
                           taaTTTTTAATAAATATTAGAAATAAAAATTTTATTTTTAAAATAAATATATTAGTAAATTAAGAAAAGCAAAAACCTCCATATTACCTATAGAGTTAAA‑‑‑‑G‑‑‑‑atatatatatatatatatatatatata                                                                                                          <  1:1310925/126‑1 (MQ=255)
                           tCATTTTTAATATATATTATTAATATAAATTTTTTTTTTATAATAAATATTTTATTTAATGTATCAAATCAAAAAACTCCATATTATCTATATAGTTAAT‑‑‑‑t‑‑‑‑atatatatatatatatatatatatata                                                                                                          <  2:2300267/128‑1 (MQ=255)
                            cATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑atatatatatatatatatatatatatat                                                                                                         >  1:3473609/1‑128 (MQ=255)
                            aaTTTTTAATAAATATTATAAATAAAAATTGTATTTTTAAAATAAATATTTTATTAAATGAACCAAACCAAAAACCTCCATATAACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑atatatatatatatatatatatatatat                                                                                                         <  1:298131/127‑1 (MQ=255)
                              tttttAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGTATATGTATCAAAGAAAAAACATCCATATTACCTATAGAGTTATT‑‑‑‑T‑‑‑‑atatatatatatatatatatatatatatat                                                                                                       <  1:3802237/128‑3 (MQ=255)
                               ttttAATAAATATTAGATATATAATTTTTTTTTTTATTATAAATATTTTAGTATATTTAGAAAAGAAAAAAACTATATATTATATATATAGTTTTT‑‑‑‑T‑‑‑‑atatatatatatatatatatatatatatatg                                                                                                      <  2:459254/128‑4 (MQ=255)
                                tttAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑atatatatatatatatatatatatatgcaagc                                                                                                     >  1:921676/1‑122 (MQ=255)
                                 ttAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑atatatatatatatatatatatatatatatatg                                                                                                    <  1:2327465/128‑6 (MQ=255)
                                  taataaATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGCACCAAAGCAAAAACATCCATATTAACTATATAGATAAT‑‑‑‑T‑‑‑‑atatatatatatatataaatatatatataaaaaa                                                                                                   >  1:2726411/1‑122 (MQ=255)
                                   aataaataTTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑ATATATATATATATATATATATATATATgatatag                                                                                                  >  1:3966002/1‑122 (MQ=255)
                                      aaataTTATAAATAAAAATAGTATTTTTAAAATTAATATTTTATTTATTTAAGAAAAGCAAAAAACTCAATATTAAATATAGAGTTAAT‑‑‑‑GAT‑‑ATATATATATATATATATATATATATATGCAAGCGa                                                                                                 <  1:1550911/128‑1 (MQ=255)
                                       aataTTATAAATAAAAATTTTTTTTTTAAAATAAATATTTTAGGAAATCAAGCATAGCAAAAACATCCATATTAAATATAGAGTTAAT‑‑‑‑GAT‑‑ATATATATATATATATATATATATATATGCAAGCGAt                                                                                                <  2:246698/128‑1 (MQ=255)
                                       aataTTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑atatatatatatatatatatatatatatataaacaatct                                                                                              >  2:2682227/1‑117 (MQ=255)
                                       aataTTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGATAAT‑‑‑‑G‑‑‑‑atatatatatatatatatatatatatattttctagctat                                                                                              >  2:2074270/1‑117 (MQ=255)
                                        atatTATATATAAAAATTATTTTTTTATAATATATATTTTATTTAAATATTATAATAAAAAACATCTATATTATATATAGAGTTATT‑‑‑‑G‑‑‑‑ATATATATATATATATATATATATATATGCAAGCGATC‑‑‑ac                                                                                          <  2:1176726/128‑2 (MQ=255)
                                        atatTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑atatatatatatatatatatatatatatatatatgcaagc                                                                                             >  2:3180405/1‑116 (MQ=255)
                                           ttATAAATAAAAATTGTATTTTTAAAATAAATTTTTTAGTATATTAATAAAATCAAAAACATCAATATTAAATATAGAGTTAATTA‑‑T‑‑‑‑ATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGc                                                                                         <  2:3478687/128‑1 (MQ=255)
                                            tAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑ATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATa                                                                                     >  1:1377128/1‑128 (MQ=255)
                                                 aTAAAAATTTTTTTTATAAAATAAATATTTTATGAAATTAATCAAAGCAAAAAACTCCATATTACCTATAGAGATAAA‑‑‑‑GATATATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATaa                                                                                    <  2:786495/128‑1 (MQ=255)
                                                 aTAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑ATATATATATATATATATATATATATATGAAAGCGATC‑‑‑AGC‑ATAAAATg                                                                                >  2:1885790/1‑128 (MQ=255)
                                                   aaaaaTTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑ATATATATATATATATATATATATATATGCTATCGATC‑‑‑AGA‑ATaaataaga                                                                              >  2:699774/1‑123 (MQ=255)
                                                    aataTTGTATTTTTAAAATAAATATTTTAGGTAATTTAGAAAAGAAAAAAACTACATATTACCTATAGAGTTAAT‑‑‑‑GAT‑‑ATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATgg                                                                               <  1:647898/125‑1 (MQ=255)
                                                    aaaaTTTTATTTTTAAAATAAATATTTTATGAAAATAATCAAATCAAAAAACTCCATATAAACAATATAGATAAT‑‑‑‑G‑‑‑‑ATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTg                                                                             <  2:1432467/128‑1 (MQ=255)
                                                    aaaaTTGTATTTTTAATATAAATATTTTAGGAAAAGAAGCAAATCAAAAACCTCCATATTAAATATAGAGTTAAT‑‑‑‑GATATATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAAt                                                                                 <  1:1815521/128‑1 (MQ=255)
                                                    aaaaTTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑ATATATATATATATATATATATATATATGTAAGCGATC‑‑‑AGC‑ATaaaa                                                                                  <  1:1492722/123‑1 (MQ=255)
                                                     aaaTTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑GATATATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATg                                                                                >  2:958255/1‑128 (MQ=255)
                                                          ttataTTTAAAATAAATATTTTATGAAATGAAGCAAAGAAAAAACATCCATATTACATATAGAGTTAATTA‑‑T‑‑‑‑ATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAAt                                                                         <  1:3590469/127‑1 (MQ=255)
                                                                 tcttacgagatttttATTAAAAGAATCAAATCAAAAAACTCAATATTAAATATAGAGTTAAT‑‑‑‑T‑‑‑‑ATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTg                                                                <  1:3670106/117‑1 (MQ=255)
                                                                 tAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑ATATATATATATATATATATATATATATGAAATCGCTC‑‑‑AGA‑ATAAAATTGTGATATTTAGATCTg                                                                >  2:3231255/1‑128 (MQ=255)
                                                                    aataaataTTTTATTAAATTATTAAAAGCAAAAAAATCTATATTAAATATATATTTAATTATAT‑‑‑‑ATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTTAAATTTAGATCt                                                                 <  2:1637351/128‑1 (MQ=255)
                                                                         atatTTTATGTATTGAATCATAGAAAAAACATCTATATTAACTATAGAGTTAAT‑‑‑‑GATATATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGtt                                                            <  1:1144180/128‑1 (MQ=255)
                                                                           atTTTAGTATATGAATCAAATATAAAACCTCCATATTAACTATATTGTTAAT‑‑‑‑G‑‑‑‑AAATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCtt                                                      <  2:2215847/128‑1 (MQ=255)
                                                                             tttATGAAATTAAGAAAATAAAAAACCTCCATATTACATATAGAGTTAAT‑‑‑‑G‑‑‑‑‑‑ATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAAt                                                  <  2:1255926/128‑1 (MQ=255)
                                                                              ttattaaaTTAATCAAAGAAAAAAAATAAATATTATCTATAGAGTTAAT‑‑‑‑GATATATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCt                                                       <  1:2920888/123‑1 (MQ=255)
                                                                              ttAGTAAATGAAGCAAACCAAAAAACTCAATATTACATATAGAGTTAAT‑‑‑‑G‑‑‑‑ATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTaat                                                   <  1:3231255/128‑2 (MQ=255)
                                                                              ttAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑GAT‑‑ATATATATATATATATATATATATATATGAAAGCGATC‑‑‑ATC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTa                                                     >  1:34152/1‑128 (MQ=255)
                                                                                  gAAATTAATAAAATCAAACACCTCAATATTTACTATATAGTTAAT‑‑‑‑GATATATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTaaa                                                   <  1:1101818/128‑1 (MQ=255)
                                                                                  gAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑GAT‑‑ATATATATATATATATATATATATATATACAAGCGATC‑‑‑ATA‑ATAAAATAGTGAAATTTAGATATGAGTTATTCTTAAAtt                                                 >  1:543408/1‑128 (MQ=255)
                                                                                   aaaTGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑ATATATATATATATATATATATATATATTCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGATTTGTTCTTAAATTTGt                                              >  2:1337848/1‑128 (MQ=255)
                                                                                     aTGAAGCAAATAAAAAAACTCAATATTATCTATAGAGTTAAT‑‑‑‑GAT‑‑ATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGt                                              <  1:855383/128‑1 (MQ=255)
                                                                                      tGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑GATATATATATATATATATATATATATATATATGCAAGCTATC‑‑‑ATC‑ATAAAATGGTGATATTTAGATGTGAGTTGGTATTAAATTTg                                               >  2:3013314/1‑128 (MQ=255)
                                                                                        aagcaaagcaAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑‑‑ATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAATTGGTGAAATTTATATCTGAGTTTGTCTTAAATTTATAGttttt                                       >  2:2971621/1‑128 (MQ=255)
                                                                                            taatcaaaAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑‑‑ATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTtttattt                                   <  2:1491122/124‑1 (MQ=255)
                                                                                            aaagcaaaAACCTCAATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑‑‑ATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTtttattt                                   <  1:3102711/128‑1 (MQ=255)
                                                                                            aaagaaaaAAACTACATATTAAATATAGAGTTAAT‑‑‑‑G‑‑‑‑ATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTtttat                                     <  2:3966002/128‑1 (MQ=255)
                                                                                              ataaaaaaaCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑ATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTtttattt                                   <  1:124883/125‑1 (MQ=255)
                                                                                              agcaaaAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑GAT‑‑ATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGTTCTTAAATTTGTAGATtttat                                     >  1:1609326/1‑128 (MQ=255)
                                                                                              agaaaaaaaCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑ATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTtttattt                                   <  1:2424651/125‑1 (MQ=255)
                                                                                               gcaaaAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑GATATATATATATATATATATATATATATATATGCAAGAGATC‑‑‑AGA‑ATAAAATGGTGATATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTttta                                      >  2:1827340/1‑128 (MQ=255)
                                                                                               gcaaaAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑‑‑‑‑‑‑ATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTTTTTTTTATAGttt                            >  2:1546883/1‑128 (MQ=255)
                                                                                                caaaAACCTCCTTATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑ATATATATATATATATATATATATATATGAAAGCTTTC‑‑‑AGG‑ATAATATTGTGATATTTTGATCTGTTTTTTTATTAAATTtttattttttattttt                                 >  1:2674318/1‑126 (MQ=255)
                                                                                                caaaAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑ATATATATATATATATATATATATATATGCAAGAGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTTttatttat                                 >  2:601315/1‑128 (MQ=255)
                                                                                                   aaaCCTCCATATTACCTATAGAGTTAAT‑‑‑‑GAT‑‑ATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATTGTGAAATTTAGATCTGAGTTTGTCTTAAATTTGTAGTTTTTATTtata                                >  1:31739/1‑128 (MQ=255)
                                                                                                   aaaCATCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑‑‑‑‑‑‑ATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTTTTATTTATAGTTTATaa                        <  2:103710/128‑1 (MQ=255)
                                                                                                    aaCCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑‑‑ATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTTTTATTTATAGTTta                           >  2:3562344/1‑128 (MQ=255)
                                                                                                     aCCTCCATATTACCTATAGAGTTAAT‑‑‑‑GATATATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAATATTGTGAAATTTAGATCTGAGTTGGTCTTATATTTATAGTTTTTATTtata                                >  1:2869228/1‑128 (MQ=255)
                                                                                                     aCCTCCATATTACCTATAGAGTTAAT‑‑‑‑GAT‑‑ATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATTGTTAAATTTATATCTGAGTTGGTATTAAATTTGTAGTTTTTATTtatatt                              >  1:2024452/1‑126 (MQ=255)
                                                                                                      caaccATATTAACTATAGAGTTAAT‑‑‑‑G‑‑‑‑ATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTTTTATTTATAGTTta                           <  1:699774/125‑1 (MQ=255)
                                                                                                        tCCATATTACCTATAGAGTTAAT‑‑‑‑GAT‑‑ATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATTGTTAAATTTAGAACTGAGTTTGTCTTAAATTTGTATTATTTATTTATAGTTta                           >  1:2520648/1‑128 (MQ=255)
                                                                                                         ccATATTACCTATAGAGTTAAT‑‑‑‑GAT‑‑ATATATATATATATATATATATATATATTCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTTTTATTTATAGTatat                          >  1:405990/1‑128 (MQ=255)
                                                                                                         caatatTATATATAGAGTTAAT‑‑‑‑T‑‑‑‑ATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTTTTATTTATAGTTTATaa                        <  2:2598401/126‑1 (MQ=255)
                                                                                                          tatatAACATATAGAGTTAAT‑‑‑‑GATATATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTTTTATTTATAGTTta                           <  1:3051699/127‑1 (MQ=255)
                                                                                                          cATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑ATATATATATATATATATATATAAATATATAAGAAATCTAAAACAATAAAATAGTGATATATATATGACTTTTTGTCTTAAATTTGTATTTtttatatagatgtta                           >  2:2755425/1‑121 (MQ=255)
                                                                                                          cATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑‑‑ATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTTTTATTTATAGTTTATAAAGt                     >  2:3306714/1‑128 (MQ=255)
                                                                                                          aatatTTCCTATAGAGTTAAT‑‑‑‑GAT‑‑ATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTTTTATTTATAGTTtata                         <  1:754843/127‑1 (MQ=255)
                                                                                                          aatatTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑ATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTTTTATTTATAGTTTATaaa                       <  1:386941/127‑1 (MQ=255)
                                                                                                           atatTACCTATAGAGTTAAT‑‑‑‑GATATATATATATATATATATATATATATATATGATAGCGATC‑‑‑ATA‑ATAAAATTGTTAAATATATATCTGAGTTTATCTTATATTTATATTTTTTATTtatattatat                          >  1:583237/1‑128 (MQ=255)
                                                                                                                aactctaGAGTTAAT‑‑‑‑GAT‑‑ATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTTTTATTTATAGTTTATAAAGTta                   <  1:3369813/123‑1 (MQ=255)
                                                                                                                  cTATAGAGTTAAA‑‑‑‑GAT‑‑ATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTTTTATTTATAGTTTATAAAGTtaat                 <  1:1942809/128‑1 (MQ=255)
                                                                                                                      agagTTAAT‑‑‑‑G‑‑‑‑ATATATATATATATATATATATATATATGCAAGCGAGC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTTTTATTTATAGTTTATAAAGTTAATAATTTa           <  1:216701/128‑1 (MQ=255)
                                                                                                                       gagTTAAT‑‑‑‑GAT‑‑ATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTTTTATTTATAGTTTATAAAGTTAATAAttt            <  2:3840284/128‑1 (MQ=255)
                                                                                                                         gTTAAT‑‑‑‑G‑‑‑‑ATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGATTTTGTATTAAATTTGTATTTTTTATTTATAGTTTATAAATTTAATAATTTatat        >  2:2869046/1‑128 (MQ=255)
                                                                                                                                     |                                                                                                                                     
TGAAACTTATTTTTGTAGTTGTAAGTTTCATTTTTAATAAATATTAGAAATAAAAATTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAGTTAAT‑‑‑‑G‑‑‑‑ATATATATATATATATATATATATATATGCAAGCGATC‑‑‑AGC‑ATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTTTTATTTATAGTTTATAAAGTTAATAATTTAGATTTTTAA  >  CP016816/437753‑438007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑