Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 254,950 C→T S457L (TCA→TTA)  JCVSYN2_01075 → RNA polymerase subunit sigma

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816254,9500CT99.5% 7917.7 / NA 2577S457L (TCA→TTA) JCVSYN2_01075RNA polymerase subunit sigma
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (1407/1157);  minor base A (7/6);  total (1414/1163)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2577 total aligned reads displayed.

TGATTCAAATGCTAAAGAATTAAAACAAGCAATTGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTCAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAAACAACCATCACCAAATAATAAATCTGGAAAAACA  >  CP016816/254825‑255074
                                                                                                                             |                                                                                                                            
tGATTCAAATGCTAAAGAATTAAAACAAGCAATTGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAg                                                                                                                            <  1:5317064/128‑1 (MQ=255)
tGATTCAAATGCTAAAGAATTAAAACAAGCAATTGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAg                                                                                                                            >  1:2284575/1‑128 (MQ=255)
  aTTCAAATGCTAAAGAATTAAAACAAGCAATTGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCt                                                                                                                          >  2:913022/1‑128 (MQ=255)
    tCAAATGCTAAAGAATTAAAACAAGCAATTGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAg                                                                                                                        >  1:3307137/1‑128 (MQ=255)
     cAAATGCTAAAGAATTAAAACAAGCAATTGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGa                                                                                                                       <  2:81156/128‑1 (MQ=255)
      aaaTGCTAAAGAATTAAAACAAGCAATTGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGaa                                                                                                                      <  2:1626244/128‑1 (MQ=255)
      aaaTGCTAAAGAATTAAAACAAGCAATTGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGaa                                                                                                                      >  2:1430616/1‑128 (MQ=255)
       aaTGCTAAAGAATTAAAACAAGCAATTGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAac                                                                                                                     <  2:428427/128‑1 (MQ=255)
       aaTGCTAAAGAATTAAAACAAGCAATTGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAac                                                                                                                     <  1:574344/128‑1 (MQ=255)
        aTGCTATAGAATTAATACAAGCAATTGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTAAGCTAGAaca                                                                                                                    <  2:3738603/128‑1 (MQ=255)
         tGCTAAAGAATTAAAACAAGCAATTGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAacac                                                                                                                   >  2:5218752/1‑128 (MQ=255)
           cTAAAGAATTAAAACAAGCAATTGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTa                                                                                                                 >  1:5243917/1‑128 (MQ=255)
            tAAAGAATTAAAACAAGCAATTGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTag                                                                                                                >  2:682969/1‑128 (MQ=255)
            tAAAGAATTAAAACAAGAAATTGAAGAATTAGATAATCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTag                                                                                                                <  2:3815393/128‑1 (MQ=255)
             aaaGAATTAAAACAAGCAATTGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTaga                                                                                                               <  1:2742014/128‑1 (MQ=255)
                  aTTAAAACAAGCAATTGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGaagaag                                                                                                          >  2:3262110/1‑128 (MQ=255)
                   ttAAAACAAGCAATTGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGt                                                                                                         >  1:763248/1‑128 (MQ=255)
                    tAAAACAAGCAATTGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGtt                                                                                                        >  1:4524145/1‑128 (MQ=255)
                    tAAAACAAGCAATTGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGtt                                                                                                        >  2:584153/1‑128 (MQ=255)
                     aaaaCAAGCATTTGAAGATTTAGATATTCATTTAGATACACCAATTGAAAAAGTTCTAAAATTTAATAATCAAATCATTAATATTAATTTAGCAAAATATGATGTAGCTAGAACACTAGAAGAAGTTg                                                                                                       <  2:5048849/128‑1 (MQ=255)
                       aaCAAGCAATTGAAGAATTAGATATTCATTTAGATACACAAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGa                                                                                                     <  2:2828407/128‑1 (MQ=255)
                         cAAGCAATTGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGaaa                                                                                                   <  2:4517144/128‑1 (MQ=255)
                         cAAGCAATTGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGaaa                                                                                                   <  1:1197239/128‑1 (MQ=255)
                           aGCAATTGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAATTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTTGAAAAg                                                                                                 >  1:5387133/1‑128 (MQ=255)
                            gCAATTGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGa                                                                                                <  1:2064958/128‑1 (MQ=255)
                            gCAATTGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGa                                                                                                >  2:5297351/1‑128 (MQ=255)
                            gCAATTGAAGAAATAGATATTCATATAGATACACGAATTGAAAAAGTAAGAAAATTTAATAATCAAATCATTAATAATTATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGa                                                                                                <  2:3456933/128‑1 (MQ=255)
                             cAATTGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTATAACACTAGAAGAAGATGGATATGaa                                                                                               >  2:2631004/1‑128 (MQ=255)
                              aaTTGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAAt                                                                                              <  1:4427702/128‑1 (MQ=255)
                                 tGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTaa                                                                                           >  1:5480015/1‑128 (MQ=255)
                                 tGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTaa                                                                                           >  1:5151058/1‑128 (MQ=255)
                                  gaagaaTTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTaaa                                                                                          <  1:2658223/128‑1 (MQ=255)
                                  gaagaaTTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGATAGAACACTAGAAGAAGTTGGAAAAGAATTaaa                                                                                          >  2:2367576/1‑128 (MQ=255)
                                   aagaaTTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAAt                                                                                         <  1:431858/128‑1 (MQ=255)
                                    agaaTTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATg                                                                                        >  1:4646757/1‑128 (MQ=255)
                                     gaaTTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGt                                                                                       <  2:5465334/128‑1 (MQ=255)
                                      aaTTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGtt                                                                                      <  1:299198/128‑1 (MQ=255)
                                      aaTTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGtt                                                                                      >  2:4850361/1‑128 (MQ=255)
                                       aTTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTAGGAAAAGAATTAAATGTTa                                                                                     >  2:2134044/1‑128 (MQ=255)
                                       aTTAGATATTCATATAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTa                                                                                     <  2:4169774/128‑1 (MQ=255)
                                        ttAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTAc                                                                                    >  1:2893751/1‑128 (MQ=255)
                                          aGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTa                                                                                  <  1:4992230/128‑1 (MQ=255)
                                           gATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTag                                                                                 <  1:176101/128‑1 (MQ=255)
                                           gATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTag                                                                                 >  1:3920930/1‑128 (MQ=255)
                                            atatTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTaga                                                                                >  1:853356/1‑128 (MQ=255)
                                                tCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGagaaa                                                                            <  1:3416688/128‑1 (MQ=255)
                                                  aTTTAGATACACCAATTGAATAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATATAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGagaaaga                                                                          <  1:3514010/128‑1 (MQ=255)
                                                   tttAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGagaaagaa                                                                         >  2:1795671/1‑128 (MQ=255)
                                                    ttAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTaa                                                                                           <  1:5091912/109‑1 (MQ=255)
                                                    ttAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAAt                                                                        >  1:1577601/1‑128 (MQ=255)
                                                          acacCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACa                                                                  <  1:2823837/128‑1 (MQ=255)
                                                             ccAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAAt                                                               >  2:1095790/1‑128 (MQ=255)
                                                              cAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAAtt                                                              >  1:1481693/1‑128 (MQ=255)
                                                                 ttGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTCGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGaa                                                           >  2:1898509/1‑128 (MQ=255)
                                                                      aaaGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCaaa                                                      <  2:1362007/128‑1 (MQ=255)
                                                                      aaaGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGAAAAATATGATTTAGCTAGAACAATAGAATAAGTTGGAAAAGAATTAAATGTTACTAGATAAAGAATCATACAAATTGATTCaaa                                                      >  1:4083605/1‑128 (MQ=255)
                                                                       aaGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCaaaa                                                     <  2:4393782/128‑1 (MQ=255)
                                                                          ttAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACt                                                  >  2:2233605/1‑128 (MQ=255)
                                                                          ttAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACGAGAGAAAGAATCAGACAAATTGAAGCAAAAACt                                                  >  1:488209/1‑128 (MQ=255)
                                                                           tAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTa                                                 <  2:120763/128‑1 (MQ=255)
                                                                            aGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTAt                                                >  1:2153919/1‑128 (MQ=255)
                                                                             gAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTAtt                                               <  2:4726735/128‑1 (MQ=255)
                                                                              aaaaTTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTa                                              <  2:3630367/128‑1 (MQ=255)
                                                                              aaaaTTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTa                                              >  1:320097/1‑128 (MQ=255)
                                                                                 aTTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGaa                                           >  2:1285448/1‑128 (MQ=255)
                                                                                  tttAATAATCAAATCATTAATAATAATTTAGCAAAATATGATATAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAAAGTTACTAGAGAAAGAATCAGACAAATTCAAGCAAACACTATAAGaaa                                          >  2:2731733/1‑128 (MQ=255)
                                                                                   ttAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGtt                                                                                                        <  2:4610801/65‑1 (MQ=255)
                                                                                    taataaTCTAATCATTAATAATAATTTAGCAAATTATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAAt                                        <  2:3978572/128‑1 (MQ=255)
                                                                                         atcaaatcaTTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTaaaa                                   <  2:3065715/128‑1 (MQ=255)
                                                                                         atcaaatcaTTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTaaaa                                   >  2:3547893/1‑128 (MQ=255)
                                                                                          tcaaatcaTTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAaac                                  >  1:2526766/1‑128 (MQ=255)
                                                                                          tcaaatcaTTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAaac                                  >  1:1839155/1‑128 (MQ=255)
                                                                                           caaatcaTTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAaaca                                 >  1:1005128/1‑128 (MQ=255)
                                                                                            aaatcaTTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAACAGAATTAAATGTTACTAGAGAAAGCATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAaacaa                                >  2:2942245/1‑128 (MQ=255)
                                                                                            aaatcaTTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAaacaa                                <  2:53764/128‑1 (MQ=255)
                                                                                            aaatcaTTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAaacaa                                <  1:663302/128‑1 (MQ=255)
                                                                                                caTTAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAAATGAAGCAAAAACTATTAGAAAATTAAAACAACCAt                            >  1:4007907/1‑128 (MQ=255)
                                                                                                  ttAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAAACAACCATCa                          >  2:1535173/1‑128 (MQ=255)
                                                                                                  ttAATAATAATTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAAACAACCATCa                          >  2:3890938/1‑128 (MQ=255)
                                                                                                   taataataaTTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAAACAACCATCAc                         <  2:333007/128‑1 (MQ=255)
                                                                                                   taataataaTTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAAACAACCATCAc                         <  1:3606018/128‑1 (MQ=255)
                                                                                                    aataataatTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAAACAACCATCAcc                        >  1:1395351/1‑128 (MQ=255)
                                                                                                      taataatTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAAACAACCATCACCaa                      >  1:3707623/1‑128 (MQ=255)
                                                                                                       aataatTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAAACAACCATCACCaaa                     >  2:2518397/1‑128 (MQ=255)
                                                                                                       aataatTTAGCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAAACAACCATCACCaaa                     >  1:1928808/1‑128 (MQ=255)
                                                                                                                gCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAAACAACCATCACCAAATAATAAATc            <  1:1114384/128‑1 (MQ=255)
                                                                                                                gCAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAAACAACCATCACCAAATAATAAATc            <  1:1659419/128‑1 (MQ=255)
                                                                                                                 cAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAATCTATTAGAAAATTAAAACAACCATCACCAAATAATAAATCt           >  1:2444495/1‑128 (MQ=255)
                                                                                                                 cAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAAACAACCATCACCAAATAATAAATCt           <  2:1694518/128‑1 (MQ=255)
                                                                                                                 cAAAATATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAAACAACCATCACCAAATAATAAATCt           >  1:3808349/1‑128 (MQ=255)
                                                                                                                  aaaaTATGATTTAGCTAGAACACTAGTAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAAACAACCATCACCAAATAATAAATCTg          <  1:3117468/128‑1 (MQ=255)
                                                                                                                  aaaaTATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAAACAACCATCACCAAATAATAAATCTg          <  2:315323/128‑1 (MQ=255)
                                                                                                                   aaaTATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAAACAACCATCACCAAATAATAAATCTgg         <  2:2000184/128‑1 (MQ=255)
                                                                                                                   aaaTATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAAACAACCATCACCAAATAATAAATCTgg         >  1:969053/1‑128 (MQ=255)
                                                                                                                    aaTATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAAACAACCATCACCAAATAATAAATCTGGa        <  2:4070566/128‑1 (MQ=255)
                                                                                                                    aaTATGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAAACAACCATCACCAAATAATAAATCTGGa        <  1:4200287/128‑1 (MQ=255)
                                                                                                                     atatGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAAACAACCATCACCAAATAATAAATCTGGaa       >  1:4793980/1‑128 (MQ=255)
                                                                                                                      tatGATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAAACAACCATCACCAAATAATAATTCTGGaaa      >  2:5143710/1‑128 (MQ=255)
                                                                                                                         gATTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAAACAACCATCACCAAATAATAAATCTGGATAAAc   >  2:495836/1‑128 (MQ=255)
                                                                                                                          aTTTAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAAACAACCATCACCAAATAATAAATCTGGAAAAACa  <  1:1316134/128‑1 (MQ=255)
                                                                                                                             |                                                                                                                            
TGATTCAAATGCTAAAGAATTAAAACAAGCAATTGAAGAATTAGATATTCATTTAGATACACCAATTGAAAAAGTTAGAAAATTTAATAATCAAATCATTAATAATAATTTAGCAAAATATGATTCAGCTAGAACACTAGAAGAAGTTGGAAAAGAATTAAATGTTACTAGAGAAAGAATCAGACAAATTGAAGCAAAAACTATTAGAAAATTAAAACAACCATCACCAAATAATAAATCTGGAAAAACA  >  CP016816/254825‑255074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: