Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 484,791 T→A intergenic (‑100/+71) JCVSYN2_02220 ← / ← JCVSYN2_02225 hypothetical protein/50S ribosomal protein L7/L12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816484,7910TA97.0% 7514.0 / 74.7 2517intergenic (‑100/+71)JCVSYN2_02220/JCVSYN2_02225hypothetical protein/50S ribosomal protein L7/L12
Reads supporting (aligned to +/- strand):  ref base T (47/28);  new base A (1217/1224);  total (1264/1253)
Fisher's exact test for biased strand distribution p-value = 3.43e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2614 total aligned reads displayed.

TATTATTTAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTT  >  CP016816/484664‑484914
                                                                                                                               |                                                                                                                             
               aGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGAAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAAtt                                                                                                              <  1:4910662/128‑1 (MQ=255)
               aGTCAAAAATCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAAtt                                                                                                              <  1:3534028/128‑1 (MQ=255)
                 tCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGAGTATTTAAATATATTTTTTTTTAATATAAAAAGCAATTaa                                                                                                            >  2:5383788/1‑128 (MQ=255)
                  cAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTaaa                                                                                                           <  1:4220692/128‑1 (MQ=255)
                  cAAAACTCATAGATTTAATCATTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTaaa                                                                                                           <  2:4317714/128‑1 (MQ=255)
                   aaaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGCGCATAGCAGAAGCTCTTAACACCTAAACTACACTCGATTATTTAATTATATTTTTTTTAATATAAAAAGCAATTAAaca                                                                                                         >  2:4499505/1‑128 (MQ=255)
                    aaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAaca                                                                                                         >  1:808152/1‑128 (MQ=255)
                     aaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAaaac                                                                                                        >  1:2696425/1‑128 (MQ=255)
                      aCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacaca                                                                                                       >  1:920858/1‑128 (MQ=255)
                      aCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacaca                                                                                                       >  1:2820128/1‑128 (MQ=255)
                       cTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacacac                                                                                                      >  1:4817907/1‑128 (MQ=255)
                       cTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacacac                                                                                                      >  2:3389925/1‑128 (MQ=255)
                          aTAGATTTAATCATTTTATAGTTGAAAAAAAAATAAGATAGAGCATAGCAGAAGCTATTAACAACTAAACTACAATAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAAcacacata                                                                                                   <  2:1846547/128‑3 (MQ=255)
                           tAGATTTTATCCTTTTATAGGTGACCAAACAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaaaa                                                                                                  <  1:5010675/128‑1 (MQ=255)
                              aTTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACt                                                                                               <  1:4632529/128‑1 (MQ=255)
                              aTTTAATCCTTTTATAGTTGAACAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACt                                                                                               <  2:5165689/128‑1 (MQ=255)
                                 tAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAc                                                                                            >  2:3155876/1‑128 (MQ=255)
                                  aaTCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTATTTTTTAATATAATAAGCAATTAAGAACACAAAAACACAcc                                                                                           >  2:278489/1‑128 (MQ=255)
                                       ttttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTa                                                                                      <  2:3611898/128‑1 (MQ=255)
                                        tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATTTAAAAAGCAATTAAACACACAAAAACTCAcaaaatat                                                                                     >  1:5194590/1‑121 (MQ=255)
                                          tataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTaa                                                                                                            >  2:811751/1‑103 (MQ=255)
                                          tataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATgg                                                                                   <  1:1306633/128‑1 (MQ=255)
                                           ataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGt                                                                                  <  1:3090959/128‑1 (MQ=255)
                                             aGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATAAAACACACAAAAACTCACCCATTATGCTGa                                                                                >  1:2275933/1‑128 (MQ=255)
                                                tGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTAACCCATTATGGTGAGtt                                                                             >  2:718810/1‑128 (MQ=255)
                                                  aCCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAATATAAAAATCAATTAAACACACAAAAACTCACACATTagtgatagtat                                                                            >  1:4464685/1‑118 (MQ=255)
                                                  aCCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCTCCCATTATGGTGAGTTTa                                                                           <  1:5496955/128‑1 (MQ=255)
                                                     aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaa                                                                        <  2:1307127/128‑1 (MQ=255)
                                                     aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaa                                                                        <  1:1007664/128‑1 (MQ=255)
                                                     aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACCAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaa                                                                        <  1:1219105/128‑1 (MQ=255)
                                                      aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                       <  1:198754/128‑1 (MQ=255)
                                                         aaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAatta                                                                    <  2:1952451/128‑1 (MQ=255)
                                                            aaGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTc                                                                 >  2:3683090/1‑128 (MQ=255)
                                                            aaGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTc                                                                 >  2:4394802/1‑128 (MQ=255)
                                                                tAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaaa                                                             >  1:2049810/1‑128 (MQ=255)
                                                                  gagCATAGCAGAAGCTCTTAACAACTGAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                           >  1:5427499/1‑128 (MQ=255)
                                                                  gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                           >  2:2384713/1‑128 (MQ=255)
                                                                  gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                           >  2:2149752/1‑128 (MQ=255)
                                                                  gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTATTATAATATAAAAAGCAATTAAACACACAAAAACTCACCCATAATGCAGAGTTTAAAAAGTAATCAAACAt                                                           >  1:1678904/1‑128 (MQ=255)
                                                                  gagCATAGAAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATAATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAATATTGTGAGTTTAAAAATTATTCAAAAAt                                                           <  1:373019/128‑1 (MQ=255)
                                                                   agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                           >  1:403980/1‑127 (MQ=255)
                                                                    gCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGGTTAAAACTTATTCAAAAAttt                                                         >  2:3818953/1‑128 (MQ=255)
                                                                       tAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGat                                                      <  1:4536646/128‑1 (MQ=255)
                                                                        aGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGatt                                                     <  1:4138426/128‑1 (MQ=255)
                                                                         gCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaaa                                                             <  1:3357340/119‑1 (MQ=255)
                                                                         gCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGatta                                                    >  2:102171/1‑128 (MQ=255)
                                                                           aGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGattatt                                                  <  1:4355874/128‑1 (MQ=255)
                                                                            gtaggtctTTACATCTAAAGTACACTCGATTATTAAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTAttt                                                 <  2:2285994/123‑1 (MQ=255)
                                                                            gAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATTGTGAGTTTAAAAATTATTCAAAAATTTGATTAttt                                                 >  2:4902927/1‑128 (MQ=255)
                                                                            gAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTAttt                                                 >  1:2906378/1‑128 (MQ=255)
                                                                            gAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTAttt                                                 >  1:5107095/1‑128 (MQ=255)
                                                                            gAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTAttt                                                 >  1:3268779/1‑128 (MQ=255)
                                                                              aGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGTTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTa                                               >  1:696167/1‑128 (MQ=255)
                                                                              aGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTa                                               <  1:4720713/128‑1 (MQ=255)
                                                                              aGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTa                                               >  2:619928/1‑128 (MQ=255)
                                                                               gCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTaa                                              <  2:1202641/128‑1 (MQ=255)
                                                                                   ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCa                                          <  1:3629978/128‑1 (MQ=255)
                                                                                   ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTAAAAAATTATACAAAAATATGATTATTTTAAATCa                                          >  2:5062670/1‑128 (MQ=255)
                                                                                     aacaacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACAAACAAAAACTCACCCATTATGTTAAGTTAAAAAATAATACAAAAATTTTATTATTTTAAATCAAt                                        >  1:3439791/1‑128 (MQ=255)
                                                                                      acaacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTaaa                                             >  1:1890154/1‑122 (MQ=255)
                                                                                       caacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTg                                      >  2:4969963/1‑128 (MQ=255)
                                                                                        aactaaactaCACTCGATTATTTAATTATATTTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTg                                      >  1:3867173/1‑128 (MQ=255)
                                                                                        aactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                     <  1:62114/128‑1 (MQ=255)
                                                                                        aactaaactaCACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAACCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                     <  2:139209/128‑1 (MQ=255)
                                                                                         actaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                    <  2:4817907/128‑1 (MQ=255)
                                                                                         actaaaataCACTCGATTATATAATAATATTTTTTTTTAATATAATAAGCAATTAAACACACATAAACTCACCCATTATGGTGATTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                    <  2:350751/128‑1 (MQ=255)
                                                                                          ctaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCCCCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTTAAg                                   >  2:4253967/1‑128 (MQ=255)
                                                                                          ctaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAg                                   <  1:2157380/128‑1 (MQ=255)
                                                                                          ctaaactaCACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAg                                   <  2:4712795/128‑1 (MQ=255)
                                                                                              actaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCAATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTcc                               >  1:2468982/1‑128 (MQ=255)
                                                                                               ctaCACTCGATTATTTAATTATATTTTTTTTTATTATAAAAAGCAATTAACCACAAAAAAACTCCCCCATTATGGTGAGTTTAAAATTTATACAAAAATTTAATTATTTTAAATCAATTGaaacacct                              >  2:1598649/1‑122 (MQ=255)
                                                                                               ctaCACTCGATTATTTAATTATATTTTTTTTTAATATACAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAATGATGATTATTTTAAATCAATAGATGCTCCa                              >  2:2887909/1‑128 (MQ=255)
                                                                                               ataCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCa                              <  2:2485687/127‑1 (MQ=255)
                                                                                                taCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAg                             >  2:298153/1‑128 (MQ=255)
                                                                                                 acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                            >  2:327505/1‑128 (MQ=255)
                                                                                                 acaatagATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACAAACAAAAACTCACACATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                            <  2:2584725/122‑1 (MQ=255)
                                                                                                  cacaCGATAATTTAATTATATTTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCtggagc                            <  1:1612193/128‑6 (MQ=255)
                                                                                                  cacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCt                           >  2:1691520/1‑128 (MQ=255)
                                                                                                   tctctATTATTTAATTAAATTTTTTTTTAATATAAAAAGCATTTAAACAATCAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCtt                          <  2:1491149/127‑1 (MQ=255)
                                                                                                   acTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCATTTAAACACACAAAAACTACCCCATTATGGTGAGTTTAAAAATTATACAAAAATTTGATAATTTAAAAACAATTGAAGATCCacatt                          >  1:5311996/1‑124 (MQ=255)
                                                                                                   acTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGcat                          >  1:3007032/1‑126 (MQ=255)
                                                                                                   acTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCtt                          >  2:52413/1‑128 (MQ=255)
                                                                                                    cTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTc                         >  1:1521182/1‑128 (MQ=255)
                                                                                                    cTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTc                         >  1:1426884/1‑128 (MQ=255)
                                                                                                       gATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAAc                      >  2:3067623/1‑128 (MQ=255)
                                                                                                        attaatTTATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCTTTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACt                     <  2:1117588/128‑1 (MQ=255)
                                                                                                           attTTATTATATTTTTTTATAATATAAAAATCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAATAATTATTCAAAAATTTGATTATTTTTAATCAATTGAAGCTCCAGCTTCAACTAAt                  <  1:3966994/128‑1 (MQ=255)
                                                                                                           attTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAt                  <  2:4011980/128‑1 (MQ=255)
                                                                                                            tttAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAtt                 <  2:2035081/128‑1 (MQ=255)
                                                                                                              tAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTTATTATTTTAAATCAATTTAATCTCCAGCTTCAACTAATTTa               >  2:927630/1‑128 (MQ=255)
                                                                                                              tAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTa               <  1:3183715/128‑1 (MQ=255)
                                                                                                              tAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTa               >  2:5523986/1‑128 (MQ=255)
                                                                                                               aaTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAg              <  1:1111755/128‑1 (MQ=255)
                                                                                                                 ttATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCTACTAATTTAGCt            >  1:2374908/1‑128 (MQ=255)
                                                                                                                     atTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATATAGCTTTCa        >  2:2680168/1‑128 (MQ=255)
                                                                                                                      tttttatattAAAAAAAAATATAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCa        >  2:413424/1‑128 (MQ=255)
                                                                                                                       ttttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGTTTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAtt      <  2:4602238/128‑1 (MQ=255)
                                                                                                                        tttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAttt     <  2:3921720/128‑1 (MQ=255)
                                                                                                                          tttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATttct   >  1:2562989/1‑128 (MQ=255)
                                                                                                                          tttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATttct   >  1:1794004/1‑128 (MQ=255)
                                                                                                                               |                                                                                                                             
TATTATTTAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTT  >  CP016816/484664‑484914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: