Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 420,163 2 bp→TT intergenic (‑446/+396) JCVSYN2_01895 ← / ← JCVSYN2_01900 30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816420,1630AT99.5% 8338.0 / NA 2686intergenic (‑446/+397)JCVSYN2_01895/JCVSYN2_0190030S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (7/7);  new base T (1440/1220);  total (1448/1238)
Fisher's exact test for biased strand distribution p-value = 7.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP016816420,1640AT98.6% 7418.4 / NA 2666intergenic (‑447/+396)JCVSYN2_01895/JCVSYN2_0190030S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (4/33);  new base T (1435/1194);  total (1439/1227)
Fisher's exact test for biased strand distribution p-value = 4.21e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2695 total aligned reads displayed.

CATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTATG  >  CP016816/420041‑420289
                                                                                                                          ||                                                                                                                               
cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                             <  1:3326723/128‑1 (MQ=255)
  ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                           >  2:6322330/1‑128 (MQ=255)
  ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                           <  2:4962444/128‑1 (MQ=255)
  ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                           >  1:3096573/1‑128 (MQ=255)
  ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATAAGTTGATATATTAGAAAACTACTAGAACTTTTTTAAAAAATATTTAATTTAAATAAc                                                                                                                           >  2:232702/1‑128 (MQ=255)
   tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACt                                                                                                                          <  1:4571745/128‑1 (MQ=255)
   tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACt                                                                                                                          >  1:626128/1‑128 (MQ=255)
     atACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGa                                                                                                                        <  2:2974261/128‑1 (MQ=255)
       aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                                      >  1:2334715/1‑128 (MQ=255)
           ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                                  >  1:2818282/1‑128 (MQ=255)
             aGTCTCATAAATCTAATTTTCTAAAATTATTTTATAATTTAAAATAATAATACTTTTATTAGTTGATATATTATAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAtttt                                                                                                                <  2:3110355/128‑1 (MQ=255)
               tctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTc                                                                                                              >  2:1136957/1‑128 (MQ=255)
                ctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCa                                                                                                             >  1:3546428/1‑128 (MQ=255)
                 tcATTAATATAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                            <  2:1526414/128‑1 (MQ=255)
                 tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                            <  2:178764/128‑1 (MQ=255)
                 tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                            <  2:5165688/128‑1 (MQ=255)
                 tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                            >  2:772094/1‑128 (MQ=255)
                   agaaaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                          >  1:6597640/3‑128 (MQ=255)
                   aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                             <  1:5070705/109‑1 (MQ=255)
                   aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                          >  1:789410/1‑128 (MQ=255)
                   aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                          >  2:4283297/1‑128 (MQ=255)
                   aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                          <  2:5492363/128‑1 (MQ=255)
                   aTAAATCTAAGTTTATAAACTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                          <  2:5251795/128‑1 (MQ=255)
                    tAAATCTAAGATTCTAAAATTATTATATCATATAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAtt                                                                                                         <  2:1674257/128‑1 (MQ=255)
                      aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                            <  1:345670/123‑1 (MQ=255)
                       aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTTAATTATTTTTCAAATTGtt                                                                                                      >  1:216662/1‑128 (MQ=255)
                          ttaGTTTCTAAAATTATTTTATCATTTAAAAAAATAATTCTTTTATTAGTTGATATATTAGAAAAGTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaaa                                                                                                   <  1:67279/126‑1 (MQ=255)
                          tAAGTTTCTAAAATTATTTTATCATTTAAAAAATTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaaa                                                                                                   <  1:533460/128‑1 (MQ=255)
                          tAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaaa                                                                                                   <  1:1578078/128‑1 (MQ=255)
                          tAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaaa                                                                                                   <  2:391800/128‑1 (MQ=255)
                            aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                                 <  1:1450947/128‑1 (MQ=255)
                             gtatCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatta                                                                                                <  2:6191223/125‑1 (MQ=255)
                             gTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAATGAATTATTTTACAAATTTTTAAatta                                                                                                >  1:5456010/1‑128 (MQ=255)
                              gttCTAAAATTATTTTATAATTTATAAAAATAATACTTTTATTAGTTGATATATTAGATAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattat                                                                                               <  2:6714610/127‑1 (MQ=255)
                               ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                              <  1:2254537/128‑1 (MQ=255)
                               ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                              >  1:2726575/1‑128 (MQ=255)
                                  tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                           <  1:1229379/128‑1 (MQ=255)
                                    aaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaa                                                                                         <  2:4096230/128‑1 (MQ=255)
                                     aaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaaa                                                                                        <  1:2062421/128‑1 (MQ=255)
                                      attattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAAt                                                                                       >  1:4000028/1‑128 (MQ=255)
                                      attattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAAt                                                                                       >  2:3656213/1‑128 (MQ=255)
                                      attattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAAt                                                                                       <  1:5849879/128‑1 (MQ=255)
                                      aatattTAATCATTAAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAAt                                                                                       <  2:3362663/126‑1 (MQ=255)
                                         attTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCAt                                                                                    >  1:5563467/1‑128 (MQ=255)
                                            ttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                                 >  2:5078433/1‑128 (MQ=255)
                                            ttATCATTTAAAAAAATAATACTTATATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                                 <  2:4438690/128‑1 (MQ=255)
                                             tATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATc                                                                                <  1:639281/128‑1 (MQ=255)
                                              aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAtat                                                                               >  2:2806744/1‑126 (MQ=255)
                                              aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                               >  2:5591411/1‑128 (MQ=255)
                                               tCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTa                                                                              >  1:4801376/1‑128 (MQ=255)
                                                 aTTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAtt                                                                            <  1:494625/128‑1 (MQ=255)
                                                   ttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTaa                                                                          <  1:5240901/128‑1 (MQ=255)
                                                     aaaaaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATc                                                                        <  2:2589017/128‑1 (MQ=255)
                                                      aaaaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCa                                                                       >  2:1866226/1‑128 (MQ=255)
                                                      aaaaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCa                                                                       <  2:5376917/128‑1 (MQ=255)
                                                         taataatACTTTTATTAGTTGATATATTAGATAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCTAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtt                                                                   <  1:6168325/127‑1 (MQ=255)
                                                         aaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtt                                                                   <  1:3667078/128‑1 (MQ=255)
                                                         aaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATATTAATAACTGAATTATTTTTCAAATTGTTAATTTATTTTCAAATCATTATCTATTAATCTAtt                                                                   >  2:6029509/1‑128 (MQ=255)
                                                          tataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAttt                                                                  <  1:196816/127‑1 (MQ=255)
                                                           ataataCTTTTCTTCGTGCACATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTGTAATTTTAATGACTGACTTATTTTTCAAATTGTTAAATTATTTTAAAATCATTATCTATTAATCAAtttt                                                                 <  2:3495759/128‑1 (MQ=255)
                                                           ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCAATTAATCAAtttt                                                                 >  2:3244411/1‑128 (MQ=255)
                                                             aataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTaa                                                               <  2:6449904/128‑2 (MQ=255)
                                                               taCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGc                                                             >  1:320348/1‑128 (MQ=255)
                                                               aacatttATTAGTTTATATATTAGAATACTACTAGTATTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTAAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGc                                                             <  1:1332332/124‑1 (MQ=255)
                                                                aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATTCt                                                            >  1:6486242/1‑128 (MQ=255)
                                                                aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCt                                                            >  1:1682492/1‑128 (MQ=255)
                                                                aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCt                                                            >  2:5696308/1‑128 (MQ=255)
                                                                aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCt                                                            >  2:1342140/1‑128 (MQ=255)
                                                                  ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCg                                                          >  1:5159932/1‑128 (MQ=255)
                                                                  ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAAGTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCg                                                          >  2:3780715/1‑128 (MQ=255)
                                                                   tttATTAGTTTATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTTAATTATTTTTCAAATTGTTAAATTATTTTAAAATCATTATCTATTAATCAATTTTATGATctt                                                         >  1:6057115/1‑126 (MQ=255)
                                                                     ttatattttttatatTATTAAAATAATATCACTTTTTTAAAAAATTTTTAATTTTAATAAATGTATTATTTTTAAAATTGTTTATTTATTTTAAAATTATTATATATTAATAAATTTTATGCTCGttt                                                      <  2:5805403/118‑1 (MQ=255)
                                                                     tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGttt                                                      >  2:592/1‑128 (MQ=255)
                                                                      atttttttatttattatTAAACTACTATAACTTTTTTAAAAAATTTTTAATTTTAATAAATTAATTATTTTTTAAATTTTTTAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGtttt                                                     <  1:4688035/117‑1 (MQ=255)
                                                                         attttatatatTATAAAAATAATAGCAATTTTTTAAAAAATTTTTAATTTTAAAAACTTAATTATTTTTAAAATTTTTAAATTATTTTCAAATAATTATCTATTAATCAATTTTATGCTCGttttttt                                                  <  1:300207/123‑1 (MQ=255)
                                                                                  atTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGtttttttttttttttt                                         >  1:5652176/1‑128 (MQ=255)
                                                                                     aGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATc                                                                                <  1:1896624/88‑1 (MQ=255)
                                                                                           ctactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGttttttttttttttttttttttaaa                                >  2:6822764/1‑120 (MQ=255)
                                                                                           ctactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTTTaaaaaa                                >  2:6598234/1‑127 (MQ=255)
                                                                                           ataatataaattttttAAAAAATTTTAAATTTTAAAAAATTAATTATTTTTAAAATTTTTAAATTATTTTAAAATAATTATATATTAATAAATTTTATTCTCGTTTTTTTTTTTTTTTTACAAAAata                               <  1:3868654/118‑1 (MQ=255)
                                                                                            tactaTCAATTTTTTAAAAAATTTTTAATTTTAATAAAAGAATTTTTTTTAAAATTTTTAAATTATTTTCAAATCATAATCTATTAATCAATTTTATGCGCGTTTTTTTTTTTTTTTTTACAAAAata                               <  1:4493170/128‑1 (MQ=255)
                                                                                              ctaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTACAAAAAtata                             >  1:917726/1‑128 (MQ=255)
                                                                                               tattaattttttAAAAAATTTTTAATTTTAATAAATTAATTTTTTTTAAAATTTTTAAATTTTTTTTAAATTATTATTTATTAATAAATTTTATTTTTGTTTTTTTTTTTTTTTTACAAAAATATAat                           <  2:216662/122‑1 (MQ=255)
                                                                                               tatcaattttttTAAAAATTTTTAATTTTAATAAATTAATTTTTTTTAAAATTGTTTAATTTTTTTAAAATAATTATATATTAATAAATTTTATTCTCTTTTTTTTTTTTTTTTTTACAAAAATATaa                            <  1:2552205/122‑1 (MQ=255)
                                                                                               atccACTTTTTTAAAAAATTTTTAATTTTAAAAACTGAATTATTTTTAAAATTTTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATTCTCGTTTTTTTTTTTTTTTTTACAAAAATATaa                            <  2:844767/125‑1 (MQ=255)
                                                                                                aGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTACAAAAATATAat                           >  1:2449894/1‑128 (MQ=255)
                                                                                                 ttaaatttttAAAAAAATTTTAATTTTTATAAATTTATTTTTTTTTAAATTTTTTAATTTTTTTAAAAAAATTAATTAATAATAAATTTTTTTGCCGTTTTTTTTTTTTTTTTTTACAAAAATATAat                           <  1:5960404/123‑1 (MQ=255)
                                                                                                 tattttttttAAAAATTTTTAAATTTTAAAAATTTATTTTTTTTAAAAATTATAAAATTTTTTAAAATTAATATAATATAATAAAATTTTTTCTCGTTTTTTTTTTTTTTTTTACAAAAATATAattt                         <  1:1791472/124‑1 (MQ=255)
                                                                                                 gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaa                                                                                         <  2:4606300/67‑1 (MQ=255)
                                                                                                 gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTACAAAAATATAattta                        >  1:5742096/1‑128 (MQ=255)
                                                                                                  cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTTAAAAAAATATAatt                          >  1:408513/1‑128 (MQ=255)
                                                                                                  cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTACAAAAtaataaata                         >  1:3766326/1‑125 (MQ=255)
                                                                                                  cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTAAAAAAATAAAattt                         >  1:1000714/1‑128 (MQ=255)
                                                                                                  cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCTTTTTTTTTTTTTTTTTAAAAAAATATTattta                        >  2:1983312/1‑128 (MQ=255)
                                                                                                     ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTTTAAAAAAATaaaattta                      >  1:707813/1‑122 (MQ=255)
                                                                                                     ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATAAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTAAAATAAAAAAattatat                      >  1:1103401/1‑124 (MQ=255)
                                                                                                        tttAAAAAATTTTTTATTTTAATAAATTTATTTTTTTTTATATTTTTAAATTTTTTTTAATTATTTATTTTTTAATATATTTTTT‑‑TTTTTTTTTTTTTTTTTTTTAAAAAAATATAATTTATTTGAtt                 <  2:2470774/128‑1 (MQ=255)
                                                                                                           aaaaaaTTTTTAATTTTAATTAATTTATTTTTTTTAAAATTTTTAATTTTTTTTAAAATTATTATATTTTAATAAATTTTAT‑‑TTTTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTaaa              <  2:4815199/128‑1 (MQ=255)
                                                                                                              aaaTTTTTAATTTTAATAAATTAATTTTTTTTAAAATTTTTAAATTTTTTTCAAATAATTATATATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAAca            <  1:4215935/128‑2 (MQ=255)
                                                                                                               aaTTTTTAATTTTAATAAATTAATTATTTTTAAATTTTTTAAATTATTTTTAAATAATTATTTATTAATAAATTTTTTTATCTTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACg            <  1:4388071/128‑1 (MQ=255)
                                                                                                                          ||                                                                                                                               
CATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTATG  >  CP016816/420041‑420289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: