Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 266,403 G→T D688Y (GAT→TAT)  JCVSYN2_01110 → guanosine‑3',5'‑bis(diphosphate) 3'‑pyrophosphohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816266,4030GT99.5% 9179.7 / NA 3099D688Y (GAT→TAT) JCVSYN2_01110guanosine‑3',5'‑bis(diphosphate) 3'‑pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base G (6/0);  major base T (1591/1485);  minor base A (10/7);  total (1607/1492)
Fisher's exact test for biased strand distribution p-value = 6.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3236 total aligned reads displayed.

AATATTGATTGAACTAATATGAAATCAAGATTAGTAGTTGTGCAGTGAAATGAAGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATGATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGTAATTACTATTAAAATTAAAAACAGTAATCAGTTAAC  >  CP016816/266277‑266527
                                                                                                                              |                                                                                                                            
         tgaACTAATATGAAATCAAGATTAGTAGTTGTGCAGTGAAATGAAGCAGTTGCTGAAAAAAATACATATACTAGAAAATTAAAATTATTTGGTATTTATAGAAACAAAATACTTTATTATATAAGTaa                                                                                                                    >  2:4334479/1‑128 (MQ=255)
         tgaACTAATATGAAATCAAGATTAGTAGTTGTGCAGTGAAATGAAGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTaa                                                                                                                    <  1:620354/128‑1 (MQ=255)
             cTAATATGAAATCAAGATTAGTAGTTGTGCAGTGAAATGAAGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAatt                                                                                                                >  1:3155101/1‑128 (MQ=255)
              tAATATGAAATCAAGATTAGTAGTTGTGCAGTGAAATGAAGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAatta                                                                                                               >  1:2534594/1‑128 (MQ=255)
                  atGAAATCAAGATTAGTAGTTGTGCAGTGAAATGAAGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTc                                                                                                           <  1:3522374/128‑1 (MQ=255)
                    gAAATCAAGATTAGTAGTTGTGCAGTGAAATGAAGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTg                                                                                                         >  1:2674285/1‑128 (MQ=255)
                     aaaTCAAGATTAGTAGTTGTGCAGTGAAATGAAGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTgg                                                                                                        >  1:2426798/1‑128 (MQ=255)
                     aaaTCAAGATTAGTAGTTGTGCAGTGAAATGAAGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTAATTATATAAGTAAAATTATTTCTgg                                                                                                        >  2:5933893/1‑128 (MQ=255)
                     aaaTCAAGATTAGTAGTTGTGCAGTGAAATGAAGCAGTAGCTGAAAAAAATACATATACTACTAAATTAAAATATATTGGTAATGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTgg                                                                                                        >  2:5191891/1‑128 (MQ=255)
                      aaTCAAGATTAGTAGTTGTGCAGTGAAATGAAGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGa                                                                                                       <  1:954840/128‑1 (MQ=255)
                         cAAGATTAGTAGTTGTGCAGTGAAATGAAGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTa                                                                                                    >  1:1726002/1‑128 (MQ=255)
                         cAAGATTAGTAGTTGTGCAGTGAAATGAAGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTa                                                                                                    <  2:3609975/128‑1 (MQ=255)
                          aaGATTAGTAGTTGTGCAGTGAAATGAAGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTaa                                                                                                   <  1:3848406/128‑1 (MQ=255)
                          aaGATTAGTAGTTGTGCAGTGAAATGAAGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTaa                                                                                                   >  1:2024432/1‑128 (MQ=255)
                           aGATTAGTAGTTGTGCAGTGAAATGAAGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTaaa                                                                                                  <  1:536638/128‑1 (MQ=255)
                            gATTAGTAGTTGTGCAGTGAAATGAAGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTaaaa                                                                                                 >  1:4879694/1‑128 (MQ=255)
                            gATTAGTAGTTGTGAAGTGAAATGAAGCAGTTGCTGATAATAATAGATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACATACTACTTTTTTATATAAGTAAAATTATTTCTGGATTaaaa                                                                                                 <  1:1221231/128‑1 (MQ=255)
                             aTTAGTAGTTGTGCAGTGAAATGAAGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAAGTATTTCTGGATTAAAAg                                                                                                >  2:6590760/1‑128 (MQ=255)
                                agtagtTGTGCAGTGAAATGAAGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTAt                                                                                             <  1:5525667/128‑1 (MQ=255)
                                  tagtTGTGCAGTGAAATGAAGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCa                                                                                           >  2:2712521/1‑128 (MQ=255)
                                     ttGTGCAGTGAAATGAAGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAatt                                                                                        >  1:1012888/1‑128 (MQ=255)
                                       gtgCAGTGAAATGAAGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAattat                                                                                      >  1:6831650/1‑128 (MQ=255)
                                       gtgCAGTGAAATGAAGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAattat                                                                                      >  1:2186626/1‑128 (MQ=255)
                                           aGTGAAATGAAGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTTGTATTGATAGAAACAAACTACATTATAATATAAGTAAATTTATTACATGATTAAAAGTATCAATTATTAAt                                                                                  >  2:2461474/1‑128 (MQ=255)
                                           aGTGAAATGAAGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAAt                                                                                  <  1:5249378/128‑1 (MQ=255)
                                            gTGAAATGAAGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATg                                                                                 >  2:1421318/1‑128 (MQ=255)
                                               aaaTGAAGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCaa                                                                              <  2:3914881/128‑1 (MQ=255)
                                                 aTGAAGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAat                                                                            >  2:1527352/1‑128 (MQ=255)
                                                    aaGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATAtt                                                                         <  2:12002/128‑1 (MQ=255)
                                                     aGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATAttt                                                                        >  2:2362589/1‑128 (MQ=255)
                                                      gaaGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTTGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATAtttt                                                                       <  1:2311708/126‑1 (MQ=255)
                                                      gCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGTATTAAAAGTATCAATTATTAATGCAAATAtttt                                                                       <  1:606123/128‑1 (MQ=255)
                                                        aGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTa                                                                     <  1:1122301/128‑1 (MQ=255)
                                                        aGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTa                                                                     <  1:3637372/128‑1 (MQ=255)
                                                        aGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTa                                                                     <  1:3734350/128‑1 (MQ=255)
                                                         gTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTAc                                                                    <  1:4714219/128‑1 (MQ=255)
                                                          ttGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACa                                                                   >  1:6702134/1‑128 (MQ=255)
                                                          ttGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACa                                                                   >  1:4485767/1‑128 (MQ=255)
                                                          ttGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACa                                                                   <  2:1952211/128‑1 (MQ=255)
                                                           tGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAg                                                                  >  1:6566917/1‑128 (MQ=255)
                                                             cTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATAATTAATGCAAATATTTTTACAGAt                                                                >  1:1422085/1‑128 (MQ=255)
                                                             atGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGAt                                                                <  2:4955937/127‑1 (MQ=255)
                                                                aaaaaaaTACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGaa                                                             <  2:6372073/128‑1 (MQ=255)
                                                                aaaaaaaTACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGaa                                                             <  1:5783752/128‑1 (MQ=255)
                                                                aaaaaaaTACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGaa                                                             <  2:2825688/128‑1 (MQ=255)
                                                                  aaaaaTACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGaaaa                                                           >  2:2067932/1‑128 (MQ=255)
                                                                  aaaaaTACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGaaaa                                                           >  2:2926767/1‑128 (MQ=255)
                                                                   aaaaTACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGaaaaa                                                          <  2:130737/128‑1 (MQ=255)
                                                                   aaaaTACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATACAAATATTATTACAGAAGaaaaa                                                          >  2:5451591/1‑128 (MQ=255)
                                                                     aaTACATATAATACTAAATTAAAATATTTTGGTATTGATAGACACAAACTAATTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATc                                                        <  2:3500708/128‑1 (MQ=255)
                                                                        aCATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCATATATTTTTACAGATGAAAAATCttt                                                     >  2:5820284/1‑128 (MQ=255)
                                                                          atatACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTttat                                                   >  2:2207944/1‑128 (MQ=255)
                                                                            ataCTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTttatta                                                 >  1:887759/1‑128 (MQ=255)
                                                                             tactacTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTtattat                                                >  1:2985773/1‑128 (MQ=255)
                                                                             tactacTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTtattat                                                >  2:473935/1‑128 (MQ=255)
                                                                             tactacTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTtattat                                                >  1:5671386/1‑128 (MQ=255)
                                                                               ctactaTATTATAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCa                                              <  2:6725909/128‑1 (MQ=255)
                                                                                 actaAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAg                                            >  2:5309523/1‑128 (MQ=255)
                                                                                 actaAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAg                                            <  1:6338725/128‑1 (MQ=255)
                                                                                    aaaTTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTgg                                         >  1:3290429/1‑128 (MQ=255)
                                                                                     aaatattttaatttAGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGa                                        <  2:333656/117‑1 (MQ=255)
                                                                                      aTTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTTGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAg                                       >  2:6465085/1‑128 (MQ=255)
                                                                                      aTTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAg                                       >  1:3955272/1‑128 (MQ=255)
                                                                                      aTTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAg                                       <  2:1778035/128‑1 (MQ=255)
                                                                                      aTTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAg                                       <  2:3711229/128‑1 (MQ=255)
                                                                                       ttAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGt                                      <  1:2773491/128‑1 (MQ=255)
                                                                                        tAAAATATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGTa                                     >  2:569636/1‑128 (MQ=255)
                                                                                         aaaaTATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTg                                          <  2:4040460/122‑1 (MQ=255)
                                                                                         aaaaTATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGTaa                                    <  2:4481386/128‑1 (MQ=255)
                                                                                           aaTATTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGTAAtt                                  <  2:6840441/128‑1 (MQ=255)
                                                                                              atTTTGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGTAATTACt                               >  2:3046575/1‑128 (MQ=255)
                                                                                              atTTTGGTATTGATAGAAACAAACTAATTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGTAATTACt                               <  1:3430630/128‑1 (MQ=255)
                                                                                                tttGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGTAATTACTat                             >  1:160245/1‑128 (MQ=255)
                                                                                                tttGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGTAATTACTat                             <  1:513904/128‑1 (MQ=255)
                                                                                                tttGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTAAAGATGATAAATCTTTATTATCAAGTGGAGTAATTACTat                             >  1:1867688/1‑128 (MQ=255)
                                                                                                 ttGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGTAATTACTatt                            >  2:3198103/1‑128 (MQ=255)
                                                                                                 ttGGTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATAATCAAGTGGAGTAATTACTatt                            >  2:4208128/1‑128 (MQ=255)
                                                                                                   ggTATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGAAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGACTAATTACTattaa                          >  2:6053891/1‑128 (MQ=255)
                                                                                                     tATTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATAAAAAATCTATATTATCTAGAGGAGAAATTAATtttaaaa                        >  1:4281631/1‑128 (MQ=255)
                                                                                                      aTTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCtt                                                      <  1:1515151/97‑1 (MQ=255)
                                                                                                      aTTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGTAATTACTattaaaat                       <  1:5015451/128‑1 (MQ=255)
                                                                                                      aTTGATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGTAATTACTattaaaat                       >  2:4583300/1‑128 (MQ=255)
                                                                                                         gATAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACa                                                                   >  2:4855415/1‑81 (MQ=255)
                                                                                                         caTAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCTATTATTAATTAAAATATTTTTACAGATGAAAAATCGTTATTATCAAGTGGAGTAATTACTagtaaaattaa                    >  2:5577539/2‑128 (MQ=255)
                                                                                                          aTAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGTAATTACTattaaaattaaa                   >  2:6239392/1‑128 (MQ=255)
                                                                                                           tAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTTGATTAAAAGTATCAATTATTAATTCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGTAATTACTAttaaaattaaaa                  <  1:4369186/128‑1 (MQ=255)
                                                                                                           tAGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGTATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGTAATTACTAttaaaattaaaa                  <  2:1084451/128‑1 (MQ=255)
                                                                                                            aGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGTAATTACTATTAAAATTaaaaa                 <  1:4599707/128‑1 (MQ=255)
                                                                                                            aGAAACAAACTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGTAATTACTATTAAAATTaaaaa                 <  2:797713/128‑1 (MQ=255)
                                                                                                              aaacaaacTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGTAATTACTATTAAAATTAAAAACa               >  2:2584643/1‑128 (MQ=255)
                                                                                                              aaacaaacTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGTAATTACTATTAAAATTAAAAACa               >  1:4113171/1‑128 (MQ=255)
                                                                                                               aacaaacTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGTAATTACTATTAAAATTAAAAACAg              <  2:1647160/128‑1 (MQ=255)
                                                                                                                   aacTACTTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGTAATTACTATTAAAATTAAAAACAGTAAt          <  1:745907/128‑1 (MQ=255)
                                                                                                                    actactTTATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGTAATTACTATTAAAATTAAAAACAGTAATc         >  2:890878/1‑128 (MQ=255)
                                                                                                                         tttATTATATAATTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGTAATTACTATTAAAATTAAAAACAGTAATCAGTTa    <  1:1325043/128‑1 (MQ=255)
                                                                                                                         tttATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGTAATTACTATTAAAATTAAAAACAGTAATCAGTTa    >  1:6437163/1‑128 (MQ=255)
                                                                                                                         tttATTATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGTAATTACTATTAAAATTAAAAACAGTAATCAGTTa    >  1:2870896/1‑128 (MQ=255)
                                                                                                                          ttattaTATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGTAATTACTATTAAAATTAAAAACAGTAATCAGTTaa   >  1:5918333/1‑128 (MQ=255)
                                                                                                                          ttattaTATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGTAATTACTATTAAAATTAAAAACAGTAATCAGTTaa   >  2:3826434/1‑128 (MQ=255)
                                                                                                                           tattatATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGTAATTACTATTAAAATTAAAAACAGTAATCAGTTAac  <  2:5084760/128‑1 (MQ=255)
                                                                                                                              |                                                                                                                            
AATATTGATTGAACTAATATGAAATCAAGATTAGTAGTTGTGCAGTGAAATGAAGCAGTTGCTGAAAAAAATACATATACTACTAAATTAAAATATTTTGGTATTGATAGAAACAAACTACTTTATGATATAAGTAAAATTATTTCTGGATTAAAAGTATCAATTATTAATGCAAATATTTTTACAGATGAAAAATCTTTATTATCAAGTGGAGTAATTACTATTAAAATTAAAAACAGTAATCAGTTAAC  >  CP016816/266277‑266527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: