Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,218 A→T intergenic (+182/‑360) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2180AT97.8% 6907.0 / ‑4.2 2366intergenic (+182/‑360)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (13/40);  new base T (1493/820);  total (1506/860)
Fisher's exact test for biased strand distribution p-value = 6.02e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2428 total aligned reads displayed.

TTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTA  >  CP016816/165093‑165347
                                                                                                                                |                                                                                                                                   
tttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaa                                                                                                                                   <  2:2220396/128‑1 (MQ=255)
 ttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaaa                                                                                                                                  <  2:473176/128‑1 (MQ=255)
  tACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaaaa                                                                                                                                 >  1:5668866/1‑127 (MQ=255)
     tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTTTATGCAAATTTTTAAAAaat                                                                                                                              >  2:5035665/1‑128 (MQ=255)
     tttAATTGAATAACTATACTAGTTTATAATATTAAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTATTAGATATTTTTTGAAAATGCAAATTTTTAAAAaat                                                                                                                              >  2:3760759/1‑128 (MQ=255)
         aTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAATAaattaat                                                                                                                          >  1:6294650/1‑128 (MQ=255)
         aTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaat                                                                                                                          <  1:6736077/128‑1 (MQ=255)
           tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                        <  2:2316273/128‑1 (MQ=255)
              aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                     >  1:1650123/1‑128 (MQ=255)
                  ctttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTg                                                                                                                 <  1:3394513/128‑1 (MQ=255)
                     tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                              <  2:4855066/128‑1 (MQ=255)
                        tAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTtaaaa                                                                                                           >  2:5796830/1‑128 (MQ=255)
                        tAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaa                                                                                                           >  2:2900441/1‑128 (MQ=255)
                                aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttt                                                                                                  >  2:1696354/1‑128 (MQ=255)
                                 atatTTAGTTTCATTAATTTATCGCACTTGTATGAATCAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttt                                                                                                 >  2:2001623/1‑128 (MQ=255)
                                  tatttattTTAATTAATTTATTGTACTTTTATTTATTATTAATAAAATAAAAATATTATATTTTTAGTTATTTTTTTTATATGAAAATTTTTAAAAAATTAATTTGAAATTGTAAAAAATAGCttttt                                                                                                <  1:1533576/128‑1 (MQ=255)
                                  tatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttt                                                                                                >  1:25887/1‑128 (MQ=255)
                                     ttAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttt                                                                                             >  2:6814070/1‑128 (MQ=255)
                                        gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttt                                                                                          >  1:273906/1‑128 (MQ=255)
                                        gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttt                                                                                          >  1:4364836/1‑128 (MQ=255)
                                         tttCATTAATTTTTCCCAATTTTATTTAATTTTAATTAAATAAAAATATTATCTTTTTTTATATTTTTTTTTTATGCAAATTTTTAAAAAATTAATTTGAACTTTTAAAAAATAGCtttttttttttt                                                                                         <  2:3006915/128‑1 (MQ=255)
                                           tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAAAAGCtattttttacaagt                                                                                       <  2:6620593/128‑7 (MQ=255)
                                             atttatttTTAGCAATTTTATTTATTATTTATAAAATAAAAATTTTTTATTTTTATATATTTTTTTTATATGCAAAATTTTTAAAAATTAATTTGAACTTGTAAAAAAAATCtttttttttttttttt                                                                                     <  2:594234/128‑1 (MQ=255)
                                             aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttt                                                                                     >  1:6082202/1‑128 (MQ=255)
                                             aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATAATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAATTGAACTTGTAAAAAATAGCtttttttttttttttt                                                                                     >  2:340903/1‑128 (MQ=255)
                                              ttAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttt                                                                                    >  1:1344460/1‑128 (MQ=255)
                                                 aTTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGCtatttttttttttttttaaa                                                                                 >  1:5000415/1‑125 (MQ=255)
                                                  tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCtttttttttttttttattaaa                                                                                >  2:3510461/1‑125 (MQ=255)
                                                   ttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTATATATTTTTTGTATATGCAAATTTTTAAAAAAATAATTTGAACTTGTAAACAATAGCtttatgttttttgtttttaaat                                                                               >  2:4482439/1‑124 (MQ=255)
                                                    tATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAAt                                                                             >  1:5445311/1‑128 (MQ=255)
                                                     ttagttaatGTATTTATTTGTAACAAAATTAAAATATTAATTTATTTTAAATTTTTTTTTTTTGGAAATTTTTAAAAAATTTATTTTTAAATGTAAAAAAAA‑‑TGTTTTTTTTTTTTTTTTTATAATgg                                                                           <  1:6629263/120‑1 (MQ=255)
                                                     atttcaattGTATTAATTATTAATAAACAAAAAATAATATATTTTTATATATTTTTTTTATATTCAAATTTTTTAAAAATTAATTTTTAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATg                                                                            <  2:4053006/121‑1 (MQ=255)
                                                      tggcaatttattGAATTTCTAAGAAACTAAAACTATTATTTTTTTACTTATTTTTTTTTTATGCAAATTTTTTAATAATTAATTTTAACTTGTATAAAATAGC‑‑GTTTTTTTTTTTTTTTTATAATGGa                                                                          <  1:315021/117‑1 (MQ=255)
                                                      tcccACTTTTTTTAATTATTTAGAAACTAAAAATTTTATTTTTTTATATTTTTTTTTTATATTCAAATTTTTAAAAAATTAAATTTAACTTTTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATgg                                                                           <  1:2462367/125‑1 (MQ=255)
                                                         cACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAAATTGTAAAAAATAGCttttttttttttttttttaaaattaaat                                                                         >  1:2241061/1‑118 (MQ=255)
                                                           cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAata                                                                      >  2:1403168/1‑128 (MQ=255)
                                                           attttattaattattaatAAACTAAAAATATTATATTTTTATATATTTTTTTTATATTCAAATTTTTAAAAAATTAAATTGAACTTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTATAATGGAAAtata                                                                    <  1:3929934/124‑1 (MQ=255)
                                                            ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGATTTTTTTTTTTTTTTTTTATAAtttaaaata                                                                     >  1:1876306/1‑120 (MQ=255)
                                                             tGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTT‑‑TAATTGAAAtatat                                                                   >  1:6838813/1‑127 (MQ=255)
                                                             tGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATaa                                                                   >  2:6732300/1‑128 (MQ=255)
                                                                aTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATATTTTAAaaataaat                                                                 >  1:5191187/1‑128 (MQ=255)
                                                                    attttttataaaataaaaataTTTTTATTTTATATATTTTTTTTATTTTTAAATTTTTTAAAAATTTATTTTTAATTTTTAAAAATATCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtttt                                                              <  1:1143239/119‑1 (MQ=255)
                                                                    aTTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTT‑‑TAATGTAAAATTAAATTTTat                                                            >  1:5918667/1‑127 (MQ=255)
                                                                      tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCTTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTat                                                            >  1:2361229/1‑127 (MQ=255)
                                                                      tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATATTTAAAATTTAAAtttttgt                                                           >  2:1503451/1‑125 (MQ=255)
                                                                      tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTAAAATAAAAATTTTatt                                                           >  2:883239/1‑126 (MQ=255)
                                                                       attaataaACAAAAACTATTATATTTTTATTAATTTTTTTTATTATCAAAATTTTTAAAAATTAATTTTAAATTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAt                                                        <  1:4509164/126‑1 (MQ=255)
                                                                       aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTAAAATATAATTTTTAGTTa                                                         >  1:4699492/1‑128 (MQ=255)
                                                                        gTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTTAATTTAAAAATAAAattttttt                                                          >  2:4615774/1‑124 (MQ=255)
                                                                         taataaACTAAAAAATTTATTTTTTAATTATTTTTTTTTTTAGTAAATTTTTTAAAAAATTAATTTTAAATTTTAAAAAAATGTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAt                                                        <  1:2896075/128‑1 (MQ=255)
                                                                          aaGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTtatttatt                                                       >  1:4176547/1‑128 (MQ=255)
                                                                           aGAAAATAAAAATTTTATATTTTTATATATTTTTTTTTTTTTTAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAATTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAttt                                                      <  1:4598768/128‑1 (MQ=255)
                                                                            taaactaaaactaTTATTTTTTTTTTTATTTTTTTTATTTTCAAATTTTTAAAAAAATAATTTGAACTTTTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAttt                                                      <  1:6409723/127‑1 (MQ=255)
                                                                               actaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTATTTtcttgt                                                >  2:209568/1‑128 (MQ=255)
                                                                                ctaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATttttttt                                                 >  1:6512890/1‑127 (MQ=255)
                                                                                 taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtt                                                                                                   >  2:6186251/1‑78 (MQ=255)
                                                                                 taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑‑‑TTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTATTTTTTTGTATaat                                           >  2:3103870/1‑127 (MQ=255)
                                                                                   aaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTTAAAAATAAATTTTATTTATTttgtttta                                               >  2:5475991/1‑125 (MQ=255)
                                                                                    atattttttgtttttATATATTTTTTGTTTATTCAAATTTTTAATATATTAATTTGTACTTTTAAAAAATA‑‑TCATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                            <  1:5378731/118‑1 (MQ=255)
                                                                                    aattattttTTTTTTTTATATTTTTTGTATATTCAAATTTTTAAAAAATTATATTTAAATTGTAAAAAAAAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTa                                             <  2:4342302/125‑1 (MQ=255)
                                                                                     actaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATGTATTTTTTTTTATaa                                            >  2:1067847/1‑128 (MQ=255)
                                                                                     aatattttTTTTATAGATATTTTTTTGAATAGCTAATTTTTAAAAATATTATTTGATATTGTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                            <  1:3621897/126‑1 (MQ=255)
                                                                                       tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATTTTTTTTTATaatt                                          >  1:176240/1‑126 (MQ=255)
                                                                                       tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATTATTGAAATATAAATTTTATGTttttttttttattatta                                         >  2:2408922/1‑119 (MQ=255)
                                                                                        attatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAATTTTTAGtttttttttttatatctga                                         >  1:3251221/1‑119 (MQ=255)
                                                                                        attatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAAAATAAATTTTGTGTTTTTTGTTGTATAATTAt                                        >  2:6066784/1‑128 (MQ=255)
                                                                                         ttatTTTTTTTTTTATTTTTTGTATTTTCAAATTTTTAAAAAAATAATTTTTACTTGT‑‑AAAATAATGTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATc                                       <  2:6475008/128‑1 (MQ=255)
                                                                                         ttatTTTTTTATATATTTTTTGTATATTGAAATTTTTAAAAAATTAATTTTTAATTTTTAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTAt                                        <  1:3043652/128‑1 (MQ=255)
                                                                                         ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAATTTAAAATTAAATTTTTGTTATTtttttgaaaaatttt                                        >  2:1854427/1‑119 (MQ=255)
                                                                                          tattattttAGTTATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTGAAATTGTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATc                                       <  1:838175/123‑1 (MQ=255)
                                                                                          tatTTTTTTATTTATTTTTTGTATTTTCAAATTTTTAAAAAATTAATTTTTACTTGTAAAAAATTGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATc                                       <  2:5237418/128‑1 (MQ=255)
                                                                                          tatTTTTTTATATTTTTTTTTTAATTTCAAATTTTTAAAAAAATAAATTTTACATTTAAAAAAAAACTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTAt                                        <  1:5295266/128‑1 (MQ=255)
                                                                                           atttttttATATTTTTTTTTTTTTTTTTAATTTTTAAATAATTTTTTTTTATTTTTAAAAAATTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAATTATCt                                      <  1:935706/125‑1 (MQ=255)
                                                                                              tttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATATAATTTTTATTTATTTTTTTTTATAATTaaaaaaa                                   >  1:3523525/1‑122 (MQ=255)
                                                                                               ttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTATGTTATTTTTTTTTATATTTATATaaa                                   >  1:6171930/1‑128 (MQ=255)
                                                                                               ttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATGAAAATATAAATTTTAGTTATTTTTTTTTATAAGttaataaaa                                  >  2:1228464/1‑120 (MQ=255)
                                                                                               ttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATAAAAATTTTATTAATTTTTTTTTATAATTATATaaaa                                  >  1:1001558/1‑128 (MQ=255)
                                                                                               ttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTAAAAATAAAAATTAAAATTTTAGTTATATTTTTTAATAATTATATaaaa                                  >  1:3706966/1‑128 (MQ=255)
                                                                                                  tttttatTTTTTTTTTTTTTAAATTTTTTAAAAATTAAATTTAAATTTTTAAAAATTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaaaa                               <  1:1997321/124‑1 (MQ=255)
                                                                                                   ttatatTTTTTTTTTATTAAAATTTTTAAAAAATTATTTTTTAATTTTTAAAAATA‑‑TATTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATa                            <  2:513005/126‑1 (MQ=255)
                                                                                                     atatTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTTAATGGAAATAAAAATTTTAGTTATTTTGTTGTATAAGTTTATAAAAAAATa                            >  2:5678559/1‑128 (MQ=255)
                                                                                                      tatTTTTTTTATATTAAAATTTTTAAAAAATTTATTTTAATTTTTAAAAAAT‑‑TTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGaa                         <  2:2506476/128‑1 (MQ=255)
                                                                                                            tttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAATTTGAAATTAAAATTTTAAGTATTTTTTTGTAAAATTATATAAAAAAATAGAAAGGa                     >  2:5352289/1‑128 (MQ=255)
                                                                                                            tttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATATATTTTATTTATTTTTTTTTCTAATTATATAAAAAAATAGaaattaa                    >  1:4050536/1‑124 (MQ=255)
                                                                                                             ttttataTTTAAATTTTTAAAAAATAAATTTTAAATTTTAAAAAATATGTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGa                     <  1:5558608/125‑1 (MQ=255)
                                                                                                               tttttattttaaTTTTTAAATAATTTATTTTTAATTTTTAAAAATTTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaa                    <  1:3821070/118‑1 (MQ=255)
                                                                                                               gTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAAAATAAATTTTATTTATTTTTTTGTATAAGTAAATAAAAAAATATAAATTaaaaaa                >  2:4194154/1‑128 (MQ=255)
                                                                                                                   tatGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTATGTATTTTTTTTTATAATTTTATAAAAAAATATAAAGTAAAAAatat             >  2:3928884/1‑128 (MQ=255)
                                                                                                                    atGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATAAAAATTTTATTTATTTTTTTTTATAATTATATAAAAAATTATAAAGTaaaaaaat              >  1:1239471/1‑126 (MQ=255)
                                                                                                                     tGCAAATTTTTAAAAAATTAATTTGCACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTATAATGTAATTATAATTTTTATGATTTTTGTTGTATAAGTTTATATAAAAATTGATAGGAAAAAat               >  1:5803686/1‑128 (MQ=255)
                                                                                                                     tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATTATGGAAATTTAAATTTTTTGTATTTTTTTTTGTAATTATATAAAAAAATATAAAGTAAAAAattt             >  2:755657/1‑126 (MQ=255)
                                                                                                                     tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGATTTTTTTTTTTTTTTTTTTAAATTTAAATATTAATTTAATTTATTTATTTATATAATATTAAAAAAAATTAAAATGGTAAAAattt             >  1:510712/1‑126 (MQ=255)
                                                                                                                      gCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTATTTTAAATATAATTTTAATTTATTTTTTTTTATAATTTTTTATAAATATATAAATaaaaaattttt            >  1:4852504/1‑123 (MQ=255)
                                                                                                                      gCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTGAAAAATAAATTTTAGGTATTTTTTTTTATAATTATAAAAAAAAATAAAAAATAAAAAATAtt            >  2:3282925/1‑128 (MQ=255)
                                                                                                                       cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAAAAAGTAAAAATAATTTTTATTTATTTTTTTGTAAAAATTTATAAAAAAAAAAAAAGTAAAAAAAAtt            >  2:5556998/1‑128 (MQ=255)
                                                                                                                        aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATTAAATTATAATTTTTATGTATTTTTTTGTATATGTTTATAAAAAAATATAAATAAAAAAAtttttta         >  1:2098785/1‑125 (MQ=255)
                                                                                                                         aaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATTTAAATATAAATTTTATTTTTTTTTTTTTATAATTATATAAAAATATAGTAATGAAAAAGTAttaaat        >  1:1429520/1‑124 (MQ=255)
                                                                                                                          aTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTATATTTTAAATATAAATTTTTGTTATTTTTTTTTATAATTATAATAAAAAAAATAAAGTAAATAATAtttt          >  2:2687417/1‑126 (MQ=255)
                                                                                                                            ttttTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATGAAAATAAAAATTTTAGTTATTTTGTTTTCTAATTATCTAAAAAAAATAATATGAATAAAAATTGTTaaa      >  2:6315570/1‑128 (MQ=255)
                                                                                                                            ttttAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAAAATAAATTTTAGTTTTTTTGTTTTATAATTATAAAAAAAAAAAAAAATAAAAAAATTTTATTaaaa     >  2:366237/1‑128 (MQ=255)
                                                                                                                                |                                                                                                                                   
TTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTA  >  CP016816/165093‑165347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: