Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 23,600 G→T I94I (ATC→ATA JCVSYN2_00085 ← 30S ribosomal protein S6

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP01681623,6000GT99.7% 9367.1 / NA 2921I94I (ATC→ATAJCVSYN2_0008530S ribosomal protein S6
Reads supporting (aligned to +/- strand):  ref base G (3/1);  major base T (1469/1440);  minor base A (4/4);  total (1476/1445)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2922 total aligned reads displayed.

CTTTCTTTTACAGCAGCTTCTTCTCTTTTAATAAATGGCTTTCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGGATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTCAACAATAACTACATGATAAAAACCTTTTTTCTT  >  CP016816/23475‑23725
                                                                                                                             |                                                                                                                             
   tctttTACAGCAGCTTCTTCTCTTTTAATAAATGGCTTTCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGtt                                                                                                                          >  2:841179/1‑128 (MQ=255)
   tctttTACAGCAGCTTCTTCTCTTTTAATAAATGGCTTTCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGtt                                                                                                                          <  2:363310/128‑1 (MQ=255)
   tctttTACAGCAGCTTCTTCTCTTTTAATAAATGGCTTTCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGtt                                                                                                                          >  2:6279495/1‑128 (MQ=255)
   tctttTACAGCAGCTTCTTCTCTTTTAATAAATGGCTTTCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATAGTTCGTAATTTTGTAttgt                                                                                                                          >  2:1736705/1‑125 (MQ=255)
       ttACAGCAGCTTCTTCTCTTTTAATAAATGGCTTTCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCt                                                                                                                      >  2:2511022/1‑128 (MQ=255)
           agcagcTTCTTCTCTTTTAATAAATGGCTTTCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTAt                                                                                                                  >  2:6412548/1‑128 (MQ=255)
            gcagcTTCTTCTCTTTTAATAAATGGCTTTCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTAtt                                                                                                                 >  1:5891520/1‑128 (MQ=255)
             cagcTTCTTCTCTTTTAATAAATGGCTTTCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTAttt                                                                                                                <  2:3987700/128‑1 (MQ=255)
              agcTTCTTCTCTTTTAATAAATGGCTTTCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTa                                                                                                               >  2:2385022/1‑128 (MQ=255)
               gcTTCTTCTCTTTTAATAAATGGCTTTCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTAGTAATTTTTTATGTTTTCTGTATTTaa                                                                                                              <  2:6676006/128‑1 (MQ=255)
                  tcttctCTTTTAATAAATGGCTTTCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAAc                                                                                                           <  1:2618242/128‑1 (MQ=255)
                    ttctCTTTTAATAAATGGCTTTCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTa                                                                                                         <  2:4204448/128‑1 (MQ=255)
                       tctTTTAATAAATGGCTTTCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAgtg                                                                                                      <  1:5728100/128‑1 (MQ=255)
                       tctTTTAATAAATGGATTTCTATAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAgtg                                                                                                      <  2:5768017/128‑1 (MQ=255)
                        ctTTTAATAAATGGCTTTCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAgtgt                                                                                                     <  2:2641579/128‑1 (MQ=255)
                         ttttatataaTGGCTTTCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGtt                                                                                                    <  1:4687514/120‑1 (MQ=255)
                         ttttAATAAATGGCTTTCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAgtgat                                                                                                    >  1:3078638/1‑126 (MQ=255)
                         ttttAATAAATGGCTTTCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGtt                                                                                                    >  2:1106786/1‑128 (MQ=255)
                         ttttAATAAATGGCTTTCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGtt                                                                                                    <  1:645087/128‑1 (MQ=255)
                         ttttAATAAATGGCTTTCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGtt                                                                                                    >  1:3635135/1‑128 (MQ=255)
                           ttAATAAATGGCTTTCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGATTTTAAAACTAGTGTTCt                                                                                                  >  1:1801024/1‑128 (MQ=255)
                                  aTGGCTTTCTCAAGAGTTTCTTATCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACg                                                                                           <  1:3903892/128‑1 (MQ=255)
                                  aTGGCTTTCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACg                                                                                           >  1:1399782/1‑128 (MQ=255)
                                   tGGCTTTCTACAGTTGTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGt                                                                                          <  2:2747046/128‑1 (MQ=255)
                                   tGGCTTTCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTTTTCTAACAACGt                                                                                          >  1:6131522/1‑128 (MQ=255)
                                    ggCTTTCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGtt                                                                                         <  1:4173129/128‑1 (MQ=255)
                                    ggCTTTCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGtt                                                                                         <  2:5624956/128‑1 (MQ=255)
                                     gCTTTCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTTTTCGTAATTTTTTATGTTTTATGTATTTAAAACTAGTGTTCTaaaaacttat                                                                                        >  2:5916137/1‑124 (MQ=255)
                                      cTTTCTAAAGTTTTTCTTTTCACGTGGTTCTTCTGCCTATATAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGtttt                                                                                       <  2:1484969/128‑1 (MQ=255)
                                      agtgcTAAAGTTTTTCTTTTCACGGTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGtttt                                                                                       <  2:2258960/124‑1 (MQ=255)
                                       tttCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGttttt                                                                                      >  1:25129/1‑128 (MQ=255)
                                       tttCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTATGTATGTTTTCTGTATTTAAAACTAGTGTTCTAAAAACGttttt                                                                                      >  2:5255079/1‑128 (MQ=255)
                                       tttCTAAAGTTTTTCTTTTCACGTTGTTATTCTTCATATTTACTCATATCTGTTTTTGAAAGAACAACTGAATGTTCTTAATTTTGTATCTTTTCTGTATTTAAAACTAATGTTCTAACAACGttttt                                                                                      >  2:1231477/1‑128 (MQ=255)
                                        ttCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTa                                                                                     <  1:228811/128‑1 (MQ=255)
                                        atCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTa                                                                                     <  2:5169658/127‑1 (MQ=255)
                                         tCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAgtg                                                                                                      <  2:3107795/110‑1 (MQ=255)
                                          cTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATc                                                                                   >  2:1981928/1‑128 (MQ=255)
                                              agctatccttttcACGTTGGTCTTCTTCATAATTAGTCATATCTGTTTTTGAAAGTACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAAtt                                                                               <  2:4297143/121‑1 (MQ=255)
                                                tttttCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAAtttt                                                                             <  1:3366752/128‑1 (MQ=255)
                                                     cttttcACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCaa                                                                        >  1:323225/1‑128 (MQ=255)
                                                      ttttcACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAAt                                                                       <  2:5431568/128‑1 (MQ=255)
                                                       tttcACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATa                                                                      <  1:6676739/128‑1 (MQ=255)
                                                         tcACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGCACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATTCg                                                                    >  2:1375558/1‑128 (MQ=255)
                                                         tcACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACg                                                                    <  1:1939376/128‑1 (MQ=255)
                                                          cACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTAGCAATACGtt                                                                  >  1:2971372/1‑128 (MQ=255)
                                                           aCGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGtt                                                                  >  2:493833/1‑128 (MQ=255)
                                                           aCGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATAGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGAAATACGtt                                                                  >  1:5384459/1‑128 (MQ=255)
                                                              ttgttCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCaa                                                               >  1:5283844/1‑128 (MQ=255)
                                                               tgttCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTg                                                                           <  2:6781594/115‑1 (MQ=255)
                                                                 ttcttcttcATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAAtt                                                            <  1:6780653/128‑1 (MQ=255)
                                                                  agtgattcATATTTAGTCATATCTTTTTTTGAAAGAAGAAGTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTTACTACGGTTTTATCAATTTTTGCAATACGTTCAAATTc                                                           <  2:3396220/123‑1 (MQ=255)
                                                                   cttcttcATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAAAAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCt                                                          >  2:1857880/1‑128 (MQ=255)
                                                                     tcttcATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCttt                                                        >  2:4648689/1‑128 (MQ=255)
                                                                        tcATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTATTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAAt                                                     >  2:4550515/1‑128 (MQ=255)
                                                                         cATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAAtt                                                    >  2:3726158/1‑128 (MQ=255)
                                                                              ttAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCTATACGTTCAAATTCTTTAATTGCTTg                                               <  1:5522053/128‑1 (MQ=255)
                                                                              ttAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTg                                               <  2:6514074/128‑1 (MQ=255)
                                                                              ttAGTAATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTg                                               <  1:4053218/128‑1 (MQ=255)
                                                                               tAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGa                                              >  1:2159760/1‑128 (MQ=255)
                                                                               tAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTATATCAATTTTTGCAATACCTTCAAATTCTTTAATTGCTTGa                                              >  2:212488/1‑128 (MQ=255)
                                                                                  tCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTATAATTGCTTGAGtt                                           >  1:5999019/1‑128 (MQ=255)
                                                                                     tatCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTa                                                       >  1:6208544/1‑113 (MQ=255)
                                                                                     tatCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTa                                        <  2:6199139/128‑1 (MQ=255)
                                                                                      atCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTg                                               <  1:4304639/120‑1 (MQ=255)
                                                                                       tCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGc                                      <  2:5507544/128‑1 (MQ=255)
                                                                                        cTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTaa                                                      <  2:1625541/111‑1 (MQ=255)
                                                                                        cTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCt                                     >  1:1010133/1‑128 (MQ=255)
                                                                                          gTTTTTGAAAGAAGATCTGATTGTTCGTAATTTTGTATGTTTTCTGAATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTc                                   <  2:2862475/128‑1 (MQ=255)
                                                                                           tttttGAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTca                                  >  1:2744446/1‑128 (MQ=255)
                                                                                                gAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGt                                                                                          <  1:3762462/67‑1 (MQ=255)
                                                                                                gAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTCAACAAt                             >  2:35251/1‑128 (MQ=255)
                                                                                                gAAAGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTCAACAAt                             >  2:386136/1‑128 (MQ=255)
                                                                                                  aaGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTCAACAATaa                           >  1:1655445/1‑128 (MQ=255)
                                                                                                   aGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTCAACAATAAc                          <  1:2407566/128‑1 (MQ=255)
                                                                                                   aGAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTCAACAATAAc                          <  2:4105527/128‑1 (MQ=255)
                                                                                                    gAACAACTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCt                                     <  1:6297046/116‑1 (MQ=255)
                                                                                                      acaacTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTCAACAATAACTAc                       >  1:111453/1‑128 (MQ=255)
                                                                                                       caacTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAATCTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTCAACAATAACTACa                      <  2:6079028/128‑1 (MQ=255)
                                                                                                        aacTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTCTTCTAACAACGTTTTTATCAATTATTGCAATACGTTCAAATTCTTTAAATGCTTGAGTTGTAGATTCTACAATAACTActt                     >  1:748489/1‑126 (MQ=255)
                                                                                                          cTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTCAACAATAACTACATGa                   <  1:2852151/128‑1 (MQ=255)
                                                                                                          cTGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTCAACAATAACTACATGa                   <  1:4943026/128‑1 (MQ=255)
                                                                                                           tGATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTCAACAATAACTACATGAt                  <  1:564934/128‑1 (MQ=255)
                                                                                                            gATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTCAACAATAACTACATGATa                 >  1:6574185/1‑128 (MQ=255)
                                                                                                            gATTGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTCAACAATAACTACATGATa                 <  2:3257475/128‑1 (MQ=255)
                                                                                                            gATGGTTCGTAATTTTGTATGTTTTCTGAATTTTATACTAGGGTGCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTCAACTATAACTACATGATa                 <  2:4800936/128‑1 (MQ=255)
                                                                                                              tttgtCGTAATTTTGTAGGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTGGAGTTGTAGCTTCAACAATAACTACATGATaaa               <  2:4429470/124‑1 (MQ=255)
                                                                                                              ttGTTCGTAATTTTGTATGTTTTCTGTATTTAAATCTATTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTCAACAATAACTACATGATaaa               <  1:3488463/128‑1 (MQ=255)
                                                                                                              ttGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTCAACAATAACTACATGATaaa               >  1:988018/1‑128 (MQ=255)
                                                                                                              ttGTTCGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTCAACAATAACTACATGATaaa               >  1:2061886/1‑128 (MQ=255)
                                                                                                                   cGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTCAACAATAACTACATGATAAAAACCt          >  1:5064710/1‑128 (MQ=255)
                                                                                                                   cGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTCAACAATAACTACATGATAAAAACCt          >  2:736239/1‑128 (MQ=255)
                                                                                                                   cGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTCAACAATAACTACATGATAAAAACCt          <  1:482476/128‑1 (MQ=255)
                                                                                                                   cGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTCAACAATAACTACATGATAAAAACCt          >  1:891418/1‑128 (MQ=255)
                                                                                                                   cGTAATTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTCAACAATAACTACATGATAAAAACCt          <  2:3002512/128‑1 (MQ=255)
                                                                                                                      aaTTTTGTATGTTTTCTTTATTTAAAACTAGTGTTCTAACAACGTTTTTATCTATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTCAACAATAACTACATGATAAAAACCtttt       <  1:4582809/128‑1 (MQ=255)
                                                                                                                      aaTTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTATAATTGCTTGAGTTGTATCTTCAACAATAACTACATGATAAAAACCttat       >  1:4431220/1‑126 (MQ=255)
                                                                                                                       aTTTTGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTCAACAATAACTACATGAt                  <  1:5193648/116‑1 (MQ=255)
                                                                                                                        ttttGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTCAACAATAACTACATGATAAAAACCtttttt     >  2:5060948/1‑128 (MQ=255)
                                                                                                                        ttttGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTCAACAATAACTACATGATAAAAACCtttttt     >  1:1513135/1‑128 (MQ=255)
                                                                                                                         tttGTATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTCAACAATAACTACATGATAAAAACCTTTTTTc    <  1:1286326/128‑1 (MQ=255)
                                                                                                                             |                                                                                                                             
CTTTCTTTTACAGCAGCTTCTTCTCTTTTAATAAATGGCTTTCTAAAGTTTTTCTTTTCACGTTGTTCTTCTTCATATTTAGTCATATCTGTTTTTGAAAGAACAACTGATTGTTCGTAATTTTGGATGTTTTCTGTATTTAAAACTAGTGTTCTAACAACGTTTTTATCAATTTTTGCAATACGTTCAAATTCTTTAATTGCTTGAGTTGTAGCTTCAACAATAACTACATGATAAAAACCTTTTTTCTT  >  CP016816/23475‑23725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: