Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 484,795 A→T intergenic (‑104/+67) JCVSYN2_02220 ← / ← JCVSYN2_02225 hypothetical protein/50S ribosomal protein L7/L12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816484,7950AT99.2% 9897.7 / NA 3236intergenic (‑104/+67)JCVSYN2_02220/JCVSYN2_02225hypothetical protein/50S ribosomal protein L7/L12
Reads supporting (aligned to +/- strand):  ref base A (20/7);  new base T (1510/1698);  total (1531/1705)
Fisher's exact test for biased strand distribution p-value = 6.04e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3318 total aligned reads displayed.

TTAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGC  >  CP016816/484670‑484918
                                                                                                                              |                                                                                                                           
     aaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaaagc                                                                                                                      >  2:1855008/1‑126 (MQ=255)
      aaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTAAATTTTTTTTTAATATAAAAAGCa                                                                                                                     >  2:2559438/1‑128 (MQ=255)
          gTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTa                                                                                                                 >  2:4716852/1‑128 (MQ=255)
           tCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTaa                                                                                                                >  2:1062682/1‑128 (MQ=255)
            cAAAACTCATCCAATTAAACCTTTTATAGATGACCCAAAAATAAGCTAGAGCAAAGCTGAAGCTCATAACAACTAAACTACACTCGATAAATTAATAATAATTTTTTTTAATATAAAAAGCAATTaaa                                                                                                               <  2:6482073/128‑1 (MQ=255)
             aaaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAATATAAAAAGCAATTaaa                                                                                                               <  2:508842/128‑1 (MQ=255)
             aaaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAac                                                                                                              >  1:3295105/1‑128 (MQ=255)
             aaaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATAATTTAATTATATTTTTTTTTAATATAAAACGCAATTAAac                                                                                                              >  2:2937994/1‑128 (MQ=255)
              aaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGACGCTCTTAACAACTAAACTACACTCGATTATTTATTTATATTTTTTTTAATATAAAAAGCAATTatccac                                                                                                            >  2:4860841/1‑123 (MQ=255)
                aCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACCCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacaca                                                                                                           <  1:5352697/128‑1 (MQ=255)
                 cTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacccac                                                                                                          >  2:5887766/1‑128 (MQ=255)
                 cTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacacac                                                                                                          <  2:2428768/128‑1 (MQ=255)
                 cTCATAGATTTAATACTTTTATAGTTGACCTAAAAATAAGATAGAGCATAGCAGAATCTCTTAACATCTTAACTAAACTCGATTATTTAATTATATTTATTTTTAATATAAAAAGCAAAGaaacaaac                                                                                                          >  2:4629819/1‑128 (MQ=255)
                         tttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTc                                                                                                  >  1:3836168/1‑128 (MQ=255)
                         tttAATACATTTATTTTTGACCAAAAAATTATAATTTTGATATCATTATCTCTTAACAACTTAACTACACTCGATTAATTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTc                                                                                                  <  1:849615/128‑1 (MQ=255)
                           tAATCCTTTTATAGTTGACCAAAAAATAATATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTAGATTATATAATTAAATTTTTTTTTTAATATAAAAAGCTATTAAAAACACaaaaaagaa                                                                                                 >  2:4970657/1‑124 (MQ=255)
                           tAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGAATATTTAATTATATATTTTTTTAATAAAAAAAGAAATAAAACAAACAAAAACTCAc                                                                                                >  1:6686755/1‑128 (MQ=255)
                             aTCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAcca                                                                                              <  2:5215306/128‑2 (MQ=255)
                              tCCTTTAATAGTTGACCAAAAAATAAGAAAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccat                                                                                             <  2:3020240/128‑3 (MQ=255)
                                cTTTTATATTTGTCCAAAAAATAATATAGAGCATTTCAGAAGATCTTAACAACTAAACTACAATCGATTATTTAATTATATTTTTTTTTAATATAAAAAGTAATTAAACACACAAAAACTCAccataa                                                                                           <  2:353985/128‑5 (MQ=255)
                                cTTTTATAGTTGACCAAAAAATAAGATAGATCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAtt                                                                                           <  1:5256998/128‑1 (MQ=255)
                                cTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAtt                                                                                           <  1:311973/128‑1 (MQ=255)
                                 ttttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTa                                                                                          <  1:421517/128‑1 (MQ=255)
                                 ttttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTa                                                                                          >  1:2158310/1‑128 (MQ=255)
                                  tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTAt                                                                                         >  1:2661140/1‑128 (MQ=255)
                                  tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTAt                                                                                         <  2:6012950/128‑1 (MQ=255)
                                   ttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCAATTATg                                                                                        >  1:5870141/1‑128 (MQ=255)
                                   ttATAGTTGAAAAAAAAATAAGATAGAGCATACCATAATCTATTAAAAAATAAACTACCCTATAATATTTAAATATATTTTATTTTAAAATAAAAAGAAATTAAAAAAACAAAAAATCAACCATTAtt                                                                                        >  2:3378979/1‑127 (MQ=255)
                                    tataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAATCAATTAAACACACAAAAACTCAACCATTATgg                                                                                       >  2:2309204/1‑128 (MQ=255)
                                    tataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATgg                                                                                       <  1:5131720/128‑1 (MQ=255)
                                    tataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGATATTAACAACTAAACTAAACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGAAATTAAACACACAAAAACTCAAACATTATgt                                                                                       >  1:2264761/1‑127 (MQ=255)
                                     ataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccataatgggt                                                                                      <  1:5980379/128‑10 (MQ=255)
                                     ataGTAGACCAAAACATACGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTATTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGt                                                                                      <  1:2798653/128‑1 (MQ=255)
                                       aGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTc                                                                                                  >  2:1679479/1‑114 (MQ=255)
                                       aGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGa                                                                                    <  2:6640671/128‑1 (MQ=255)
                                       aGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACACATTATGGtaa                                                                                    >  1:2950319/1‑126 (MQ=255)
                                          tGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGtt                                                                                 >  1:3415702/1‑128 (MQ=255)
                                            aCCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTTATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTa                                                                               <  2:5708215/128‑1 (MQ=255)
                                              cAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaa                                                                             <  1:1164614/128‑1 (MQ=255)
                                               aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaa                                                                            <  2:4023778/128‑1 (MQ=255)
                                                aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                           <  2:1181425/128‑1 (MQ=255)
                                                  aaaTAAGATAGAGCATAGCAGAATCTCTAAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAatt                                                                         <  1:962114/128‑1 (MQ=255)
                                                  aaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAatt                                                                         <  1:5035903/128‑1 (MQ=255)
                                                   aaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAac                                                                                                              <  2:1542712/90‑1 (MQ=255)
                                                      aaGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTc                                                                     >  1:2542092/1‑128 (MQ=255)
                                                      aaGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTACCCCATTATGGTGAGTTTAAAAATTATTc                                                                     >  1:3023945/1‑128 (MQ=255)
                                                       aGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTGATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCa                                                                    >  2:5325429/1‑128 (MQ=255)
                                                         aTAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaa                                                                  <  2:4404029/128‑1 (MQ=255)
                                                            gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGGTTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACACATTATGGAGAGTTTAAAAATTATTCAAAAAt                                                               >  1:5748631/1‑128 (MQ=255)
                                                            gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTTATAATTATTCaataaa                                                               >  2:6770677/1‑127 (MQ=255)
                                                            gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                               <  1:1002607/128‑1 (MQ=255)
                                                            gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                               >  1:1910535/1‑128 (MQ=255)
                                                             agCATAGCAGAAGCTCTTTACAACTAAACTACACTCGAATATTTAATTATATTATTTTTTAATATAAAAAGCTATTAAACACCCAAAATCTCACCCATTATGGTGATTTTTAAAATTATTCTAAAAtt                                                              >  1:2038602/1‑128 (MQ=255)
                                                             agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTATTATAAAAAGCAATTAAACACACAAAAACTCACCCATAATGGTGAGTTTAAAAATAATTCAAAAAtt                                                              >  1:1770745/1‑128 (MQ=255)
                                                             agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                              >  1:3693881/1‑128 (MQ=255)
                                                              gcatagcataAGCTCTTAACAACTAAACTACCCTCGATTATTTAATTATATTTTTTTTTAATATATAAAGCAATTAAACACACAAAAACTCCCCCATTATGTTGAGATTAAAAATTAATCaaaatatt                                                             >  2:2138384/1‑124 (MQ=255)
                                                              gCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAATTTATTCAAAAAttt                                                             >  2:3112724/1‑128 (MQ=255)
                                                              gCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAttt                                                             >  1:5508177/1‑128 (MQ=255)
                                                               cATATCATAAGCTCTTAACAAATAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAAAACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTg                                                            <  1:647414/128‑1 (MQ=255)
                                                                aTAGCAGAAGCTCTTATCAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGa                                                           <  1:1616263/128‑1 (MQ=255)
                                                                aTAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGa                                                           <  2:5473695/128‑1 (MQ=255)
                                                                aTAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGa                                                           <  1:5440524/128‑1 (MQ=255)
                                                                aTAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGa                                                           <  1:3956888/128‑1 (MQ=255)
                                                                  aGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGatt                                                         >  1:1508099/1‑128 (MQ=255)
                                                                  aGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTAACCCATTAAGGTTAGTTTAAAAATTATTCAAAAATTTGatt                                                         >  2:2669261/1‑128 (MQ=255)
                                                                   gCAGAAGCTCTTAACTACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCAATTATGGTTAGTTTAAATCTTATTCAAAAAttacctta                                                        >  1:3560505/1‑122 (MQ=255)
                                                                   gCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATCGTGAGAATAAAAAATATACAAAAATTTGatta                                                        >  2:6345954/1‑128 (MQ=255)
                                                                     aGAAGCTCATAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGattatt                                                      <  2:3930414/128‑1 (MQ=255)
                                                                      gAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACACATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTAttt                                                     >  1:6780196/1‑128 (MQ=255)
                                                                            ttttactactatacttCAATCTATTATTTTATTTTATTTTTTTTTTATATAAAAAGCAATTAAACACACATAATCTCACCCATTATGGTGATTTTAAAAATTATTCAAAAATTTGATTATTTTAAATc                                               <  2:1283321/121‑1 (MQ=255)
                                                                            ctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAttt                                                             >  1:6310529/1‑114 (MQ=255)
                                                                             ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCa                                              >  1:1393948/1‑128 (MQ=255)
                                                                             ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATATTAAATCa                                              >  2:1422078/1‑128 (MQ=255)
                                                                              tAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCaa                                             >  1:4065708/1‑128 (MQ=255)
                                                                                acaacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAtt                                           <  1:4792231/128‑1 (MQ=255)
                                                                                 caacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTg                                          <  2:753408/128‑1 (MQ=255)
                                                                                  aactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                         <  1:5635361/128‑1 (MQ=255)
                                                                                  aactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAGTATGGTGAGATTAAAAATTATTCAAAACTTAGATTATTTTAAATCAATTGa                                         >  2:4513480/1‑128 (MQ=255)
                                                                                   actaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                        >  2:4151125/1‑128 (MQ=255)
                                                                                     taaactaCACACGATTAATTAAATATAATTTTTTTTAATATAAAAAGCAATAAAACACACAAAAACTCACCAATTATCGTTAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGc                                      >  2:5570719/1‑128 (MQ=255)
                                                                                        actaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTcc                                   >  2:3249964/1‑128 (MQ=255)
                                                                                           acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                                >  1:1282759/1‑128 (MQ=255)
                                                                                           acacTCGATTATTAAATTATATTTTTTTTTAATATAAAAAGAAATTAAAAACACAAAAACTCACAAATTATCTTGAGTATAAAAATTATTCAAAAATTAGATTATATTAAATCAATTGAATCttaata                                >  2:6123627/1‑123 (MQ=255)
                                                                                               tagATTATTTAATTATTTTTTTTTTTAATATAAAAAGCAATTAATCACACAAATACTCATCTATTATGGTGAGTTTAAATATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGTTCCAGATTCa                            <  1:466342/126‑1 (MQ=255)
                                                                                                 gATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAAc                          >  2:3813698/1‑128 (MQ=255)
                                                                                                   ttattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACACAATATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAACTACAGCATCAACTa                        >  2:6227220/1‑128 (MQ=255)
                                                                                                     attTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAt                      <  1:4307878/128‑1 (MQ=255)
                                                                                                       ttAAATATATTTTTTTTTAATATAAAAAGCAATTATACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATCCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAttt                    <  2:2792972/128‑1 (MQ=255)
                                                                                                        tAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTa                   <  2:2007039/128‑1 (MQ=255)
                                                                                                         atttATTTTTTTTTTTAATATATATAGTAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAg                  <  2:4285134/126‑1 (MQ=255)
                                                                                                         aaTTATATTTTTTTTTAATATATAAAGCAATTAAACACACTAAAACTCACCCATTATGGTGAGTTTAATAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAg                  <  1:607230/128‑1 (MQ=255)
                                                                                                         aaTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAg                  <  1:4940322/128‑1 (MQ=255)
                                                                                                           ttATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCt                >  2:871283/1‑128 (MQ=255)
                                                                                                             atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAATAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt              >  1:713077/1‑128 (MQ=255)
                                                                                                             atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt              >  1:4426539/1‑128 (MQ=255)
                                                                                                                tttttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAt           >  1:4575340/1‑128 (MQ=255)
                                                                                                                tttttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAt           <  1:6229565/128‑1 (MQ=255)
                                                                                                                 ttttttttaATATAAAAATCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAtt          <  2:3666660/128‑1 (MQ=255)
                                                                                                                 ttttttttaATATAAAAAGCAATTAAACACACAAAAACTCAAACATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAtt          <  1:2424387/128‑1 (MQ=255)
                                                                                                                    tttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATttct       >  1:6589190/1‑128 (MQ=255)
                                                                                                                              |                                                                                                                           
TTAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGC  >  CP016816/484670‑484918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: