Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 273,833 C→T intergenic (‑16/+9) JCVSYN2_01135 ← / ← JCVSYN2_01140 dihydroxyacetone kinase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816273,8330CT99.4% 10556.6 / NA 3362intergenic (‑16/+9)JCVSYN2_01135/JCVSYN2_01140dihydroxyacetone kinase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (1867/1469);  minor base A (13/8);  total (1882/1480)
Fisher's exact test for biased strand distribution p-value = 6.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3427 total aligned reads displayed.

TCTCCATCAGGAACTGGAAAGACATTTAATTTATCTATTTGTGGATAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTCTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGAATCTGAATTTCTTTTAAAATATCTAAAATATTAA  >  CP016816/273708‑273957
                                                                                                                             |                                                                                                                            
  tcCATCAGGAACTGGAAAGACATTTAATTTATCTATTTGTGGATAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTAt                                                                                                                          >  1:5468104/1‑128 (MQ=255)
  tcCATCAGGAACTGGAAAGACATTTAATTTATCTATTTGTGGATAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTAt                                                                                                                          >  2:4205916/1‑128 (MQ=255)
     agcatgcaCTGGAAAGACATGAAATTTATCTCTTTGTGGAGAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTAtttt                                                                                                                       <  2:3725104/121‑1 (MQ=255)
      tgaGGAAGTGGATAGACATTTAATTTATCTATTTGTGGATAATGATTGTATAGATTATTCACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTAttttt                                                                                                                      <  1:5140231/126‑1 (MQ=255)
      tCAGGAACTGGAAAGACATTTAATTTATCTATTTGTGGATAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTAttttt                                                                                                                      <  2:6695346/128‑1 (MQ=255)
       cAGGAACTGGAAAGACATTTAATTTATCTATTTGTGGATAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATATTTCTAATTTTTTCATTTTACTCAGATCTTTTTTAtttttt                                                                                                                     >  1:4858845/1‑128 (MQ=255)
              tGGAAAGACATTTAATTTATCTATTTGTGGATAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGCTCTTTTATATTTTTTTAGATTa                                                                                                              >  1:4525890/1‑128 (MQ=255)
              tGGAAAGACATTTAATTTATCTATTTGTGGATAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTa                                                                                                              <  1:6643189/128‑1 (MQ=255)
                gAAAGACATTTAATTTATCTATTTGTGGATAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTaaa                                                                                                            >  1:5775737/1‑128 (MQ=255)
                 aaaGACATTTAATTTATCTATTTGTGGATAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAAt                                                                                                           <  2:5164985/128‑1 (MQ=255)
                 aaaGACATTTAATTTATCTATTTGTGGATAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAAt                                                                                                           <  1:3220915/128‑1 (MQ=255)
                   aGACGTTTAATTTATCTATTTGTGGATAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCt                                                                                                         >  1:586249/1‑128 (MQ=255)
                   aGACATTTAATTTATCTATTTGTGGATAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCt                                                                                                         >  1:1804828/1‑128 (MQ=255)
                   aGACATTTAATTTATCTATTTGTGGATAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCt                                                                                                         >  1:5619416/1‑128 (MQ=255)
                    gACATTTAATTTATCTATTTGTGGATAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCtt                                                                                                        >  2:4816172/1‑128 (MQ=255)
                    gACATTTAATTTATCTATTTGTGGATAATGATTGTATAGATTATTAACTCCACATGATATCATGTTTTTAAATAATTCTAATTAATTCATTTTACTCAGAACTATTTTATTTTTTTAGATTAAATCta                                                                                                        >  1:6214758/1‑127 (MQ=255)
                     aCATTTAATTTATCTATTTGTGGATAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTc                                                                                                       >  1:4980632/1‑128 (MQ=255)
                      cATTTAATTTATCTATTTGTGGATAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTATATTAAATCTTca                                                                                                      >  2:1500634/1‑128 (MQ=255)
                       atttaatttaTCTATTTGTGGATAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGCTTAAATCTTcaa                                                                                                     >  2:3307572/1‑128 (MQ=255)
                         ttaatttaTCTATTTGTGGATAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTcaaca                                                                                                   <  1:3518796/128‑1 (MQ=255)
                         ttaattcaTCTATTTGTGGATAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTcaaca                                                                                                   >  2:5869271/1‑128 (MQ=255)
                         agaatttaTCTATTTTTGGATAATTATTGTATAGATTATTCACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTcaaca                                                                                                   <  2:6173406/126‑1 (MQ=255)
                          tactttaTCTATTTGTGGATAATGATTGTATATATTAATAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTcaacaa                                                                                                  <  2:6060958/125‑1 (MQ=255)
                            atttaTCTATTTGTGGATAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAAtt                                                                                                >  1:4361861/1‑128 (MQ=255)
                            atttaTCTATTTGTGGATAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTCTTTTAGATTAAATCTTCAACAAtt                                                                                                <  2:3859651/128‑1 (MQ=255)
                              ttaTCTATTTGTGGATACTGATGCTATAGATTATTCACTCCACTTGTTATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTAc                                                                                              <  2:2869521/128‑1 (MQ=255)
                               taTCTATATGTGGATAATGAATGTATAGATTATTAACTCCACTTGTTATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACa                                                                                             <  2:3391854/128‑1 (MQ=255)
                                 tCTATTTGTGGATAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATc                                                                                           <  2:1071499/128‑1 (MQ=255)
                                 tCTATTTGTGGATAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATc                                                                                           >  1:1000410/1‑128 (MQ=255)
                                   tATTTGTGGATAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCaa                                                                                         <  2:445874/128‑1 (MQ=255)
                                      ttGTGGATAATGATTGTATAGATTATTAACTCCCCTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATATTCAACAATTACATCAAAtt                                                                                      >  2:796839/1‑128 (MQ=255)
                                      ttGTGGATAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACtt                                                                                      <  1:2050714/128‑1 (MQ=255)
                                      ttGTGGATAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATAAACtt                                                                                      >  2:3224893/1‑128 (MQ=255)
                                       tgtgGATACTGATTGTATAGATTATTAACTCCACTTGTTATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACttt                                                                                     <  2:525280/128‑1 (MQ=255)
                                         tgGATAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTAt                                                                                   >  2:2778915/1‑128 (MQ=255)
                                               aTGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTAATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTCCATAAACTTAATGTTCaa                                                                             >  2:361468/1‑128 (MQ=255)
                                                  aTTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAatat                                                                          >  2:1720617/1‑128 (MQ=255)
                                                      tataGATTTTTTACTCCAATTGTGATATTGTTTTTTAATAAATCTAATATTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACACTTACATCAAGTTTATGTTCAATattatt                                                                      <  1:4137568/128‑1 (MQ=255)
                                                      tataGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATattatt                                                                      >  1:526924/1‑128 (MQ=255)
                                                      tataGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATattatt                                                                      <  2:5475816/128‑1 (MQ=255)
                                                      tataGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATattatt                                                                      <  2:348123/128‑1 (MQ=255)
                                                      tataGAATATTAACTCGACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATattatt                                                                      <  2:5733602/128‑1 (MQ=255)
                                                       ataGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTAttt                                                                     >  1:969056/1‑128 (MQ=255)
                                                        taGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTAtttt                                                                    <  1:6785956/128‑1 (MQ=255)
                                                         aGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTAttttt                                                                   >  1:4000297/1‑128 (MQ=255)
                                                         aGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTAttttt                                                                   >  2:2398960/1‑128 (MQ=255)
                                                          tattcttAACTCCTCATGTGATCATGTTTTTTAATAATTCTATTTTTTTCATTTTACTAAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTa                                                                  <  1:3418978/127‑1 (MQ=255)
                                                          gATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTa                                                                  <  1:4648183/128‑1 (MQ=255)
                                                          gATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTa                                                                  >  1:2538454/1‑128 (MQ=255)
                                                              attaACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAAt                                                              >  2:1627668/1‑128 (MQ=255)
                                                               ttaACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTATATTAAATCTTCAAAAATTACATCAACTTTATGTTCAATATTATTTTTAAAAtt                                                             >  2:5947778/1‑128 (MQ=255)
                                                               ttaACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAAtt                                                             >  2:3110895/1‑128 (MQ=255)
                                                                   cTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCttt                                                         >  2:4006052/1‑128 (MQ=255)
                                                                    tCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAACTCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCtttt                                                        >  1:330615/1‑128 (MQ=255)
                                                                     ccACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTc                                                       <  1:2409159/128‑1 (MQ=255)
                                                                      cACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCt                                                      >  1:6105939/1‑128 (MQ=255)
                                                                        cTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTaa                                                    >  1:157216/1‑128 (MQ=255)
                                                                         ttGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAAt                                                   >  2:6269725/1‑128 (MQ=255)
                                                                            tgATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCa                                                >  2:1375482/1‑128 (MQ=255)
                                                                              aTCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCata                                              <  1:774874/128‑1 (MQ=255)
                                                                               tCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAAtatt                                                                         <  2:4331379/100‑1 (MQ=255)
                                                                               tCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCatat                                             >  2:5575775/1‑128 (MQ=255)
                                                                                cATGTTTTTTAATACTTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATAtt                                            <  1:2759848/128‑1 (MQ=255)
                                                                                cATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATAtt                                            >  1:1207235/1‑128 (MQ=255)
                                                                                 aTGTTTTTTAATAATTCTAATTTTTTCATTTTACTCATATCTTTTTTATTTTTTTAGATTAAATCTTCAAAAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATAttt                                           <  1:3637331/128‑1 (MQ=255)
                                                                                  tGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTa                                          <  1:1446943/128‑1 (MQ=255)
                                                                                     tttttAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATg                                       <  1:132598/128‑1 (MQ=255)
                                                                                     tttttAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATg                                       >  1:3901063/1‑128 (MQ=255)
                                                                                       tttAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCg                                     >  1:2846070/1‑128 (MQ=255)
                                                                                        ttAATAATTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTTGATTAAATCTTCAACAATTACATCGACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGa                                    <  2:5030142/128‑1 (MQ=255)
                                                                                        tgactaatTCTACTTTGTTCATTTTCCTCAGATCTTTTTTATTGTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTCTTTTTAAAATTCTTTTCTAATTCATATTTAATGCGa                                    <  1:1659106/124‑1 (MQ=255)
                                                                                         taataaATCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGaa                                   <  1:2643485/128‑1 (MQ=255)
                                                                                          aataatTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTc                                                                               >  2:1952901/1‑83 (MQ=255)
                                                                                          aataatTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGAAt                                  >  2:2519157/1‑128 (MQ=255)
                                                                                           ataatTCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGAATc                                 >  1:599282/1‑128 (MQ=255)
                                                                                               ttCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGAATCTGaa                             <  1:3110157/128‑1 (MQ=255)
                                                                                                tCTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGAATCTGAAt                            >  2:6531281/1‑128 (MQ=255)
                                                                                                 cTAATTTTTTCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGAATCTGAAtt                           <  1:650838/128‑1 (MQ=255)
                                                                                                  tAATTTTTTCATTTTACTCAGATCTTTTTTATTTATTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGAATCTGAAttt                          >  2:2113860/1‑128 (MQ=255)
                                                                                                     ttttttCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGAATCTGAATTTCtt                       <  2:4924864/128‑1 (MQ=255)
                                                                                                     ttttttCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGAATCTGAATTTCtt                       <  2:2270829/128‑1 (MQ=255)
                                                                                                     ttatttCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGAATCTGAATTTCtt                       <  2:1848852/125‑1 (MQ=255)
                                                                                                      tttttCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGAATCTGAATTTCttt                      >  2:4124270/1‑128 (MQ=255)
                                                                                                      attttCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGAATCTGAATTTCttt                      <  1:1926560/127‑1 (MQ=255)
                                                                                                       ttttCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATTCGAATCTGAATTTCtttt                     >  1:4255228/1‑128 (MQ=255)
                                                                                                        tttCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGAATCTGAATTTCTTTTa                    >  1:2294887/1‑128 (MQ=255)
                                                                                                         ttCATTTTACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGAATCTGAATTTCTTTTaa                   >  1:3750273/1‑128 (MQ=255)
                                                                                                          tCATTTTACTCAGATCTTTTTTATTATTTTAGATTAAATCTTCAACAATTACATCAACTTTATGATCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGAATCTGAATTTCTTTTaaa                  <  2:6376765/128‑1 (MQ=255)
                                                                                                            aatttACTCACATCTTTTTAATTATTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGAATCTGAATTTCTTTTAAAat                <  2:2994271/126‑1 (MQ=255)
                                                                                                             ttttACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGAATCTGAATTTCTTTTAAAata               <  1:299014/128‑1 (MQ=255)
                                                                                                              tttACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGAATCTGAATTTCTTTTAAAatat              <  2:455862/128‑1 (MQ=255)
                                                                                                                tACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACCTCAACTATATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGAGAATCTGAATTTCTTTAAAAATATCt            >  1:1100943/1‑128 (MQ=255)
                                                                                                                tACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGAATCTGAATTTCTTTTAAAATATCt            >  1:2173633/1‑128 (MQ=255)
                                                                                                                tACTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGAATCTGAATTTCTTTTAAAATATCt            >  1:634878/1‑128 (MQ=255)
                                                                                                                  cTCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGAATCTGAATTTCTTTTAAAATATCt            <  2:266109/126‑1 (MQ=255)
                                                                                                                   tCAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGAATCTGAATTTCTTTTAAAATATCTaaa         >  2:1207720/1‑128 (MQ=255)
                                                                                                                    cAGATCTTTTTTATTTTTTTAGCTTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGAATCTGAATTTCTTTTAAAATATCTaaaa        <  2:6839177/128‑1 (MQ=255)
                                                                                                                    cAGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGAATCTGAATTTCTTTTAAAATATCTaaaa        >  1:4756693/1‑128 (MQ=255)
                                                                                                                     aGATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGAATCTGAATTTCTTTTAAAATATCTAAAat       <  2:538070/128‑1 (MQ=255)
                                                                                                                      gATCTTTTTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGAATCTGAATTTCTTTTAAAATATCTAAAata      <  2:4651963/128‑1 (MQ=255)
                                                                                                                             |                                                                                                                            
TCTCCATCAGGAACTGGAAAGACATTTAATTTATCTATTTGTGGATAATGATTGTATAGATTATTAACTCCACTTGTGATCATGTTTTTTAATAATTCTAATTTTTTCATTTTACTCAGATCTTTCTTATTTTTTTAGATTAAATCTTCAACAATTACATCAACTTTATGTTCAATATTATTTTTAAAATTCTTTTCTAATTCATATTTAATGCGAATCTGAATTTCTTTTAAAATATCTAAAATATTAA  >  CP016816/273708‑273957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: