Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2500T.58.8% 489.6 / inf 1976intergenic (+214/‑328)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base T (432/347);  new base . (815/295);  total (1256/720)
Fisher's exact test for biased strand distribution p-value = 6.79e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2444 total aligned reads displayed.

ATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAAC  >  CP016816/165123‑165377
                                                                                                                                |                                                                                                                                
  aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATTCAAA‑TTTTTAAAAAATTAATTTGAAATTTTAAAAAATAGAttt                                                                                                                                >  1:6205322/1‑128 (MQ=255)
   atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAATAAATAGAtttt                                                                                                                               >  1:5842372/1‑128 (MQ=255)
     atTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTATTAAAAAATTAATTAGAACTTGTAAAAAATAGCtttttt                                                                                                                             >  1:1323456/1‑128 (MQ=255)
        tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                                                          >  1:3442195/1‑128 (MQ=255)
          ttttCATTAATTTATCGCAAATGTATGAATTAGTAAGAAACTAAAACAATTATCTTTTTACATAATTTTTGTATATGCAAA‑TTTTTAAAAAAATAATTTGAACATGTAAAAAAAACCttttttttttt                                                                                                                        <  1:1233662/127‑1 (MQ=255)
            ttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTCAACTTGTAAAAAATAGCttattttttttat                                                                                                                      >  1:4503972/1‑126 (MQ=255)
             tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAACCTATTATCGTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAATAAATAAATTTGAACTTGTAAAAAATATCtttttttttttttt                                                                                                                     >  2:4426577/1‑128 (MQ=255)
             tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttt                                                                                                                     >  1:5618113/1‑128 (MQ=255)
                ttAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttt                                                                                                                  >  2:1561685/1‑128 (MQ=255)
                    tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttatttttataaa                                                                                                              >  1:2219079/1‑123 (MQ=255)
                    tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttattttttttttattttta                                                                                                              >  2:6611336/1‑126 (MQ=255)
                    tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGAAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCTTTTTTTTTTTTTTTTtataa                                                                                                              >  1:1422044/1‑127 (MQ=255)
                     ttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttaaaaat                                                                                                             >  1:2818697/1‑122 (MQ=255)
                       aTCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttatattg                                                                                                           >  2:4574202/1‑122 (MQ=255)
                          gCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttttttaatga                                                                                                        >  1:858003/1‑120 (MQ=255)
                             cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttattattataata                                                                                                     >  1:5501758/1‑116 (MQ=255)
                             cTTGTATGAATTAGTAAGAAACTAAAACGATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTtaattttaatat                                                                                                     >  2:5810733/1‑118 (MQ=255)
                             attGTATTAATTATTAAAAAATAAAAATTTTTTTTTTTTTTTTTTTTTTTTTTATTTGAAAA‑TTTTAAAAAAATTAATTTTAAATTTTAAAAAA‑A‑‑TCCTTTTTTTTTTTTTTTTATAATGGAAAtata                                                                                                  <  2:481964/127‑1 (MQ=255)
                              ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGTAAata                                                                                                    >  2:5346907/1‑128 (MQ=255)
                              ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATTTAAataa                                                                                                   >  2:4714723/1‑127 (MQ=255)
                                 tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTTTAAAAAATAGCTTTTTTTTTTTTTTTTTTT‑TAATGAAAATATaaa                                                                                                >  2:5230889/1‑128 (MQ=255)
                                 tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTTAAATATAAAtt                                                                                              >  1:3927994/1‑128 (MQ=255)
                                   ttaatttttAAGTAAATTAAACTATTATTTTTTTTTATTTTTTTTTTTTTTGGAAA‑TTTTTTAAAAAATTATTTTAAATTTTAAAAAA‑AATGTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                                              <  2:185357/120‑1 (MQ=255)
                                        tatttaGAAACTAAAACTATTTTCTTTTTATTTTTTTTTTTTTATTTTAAA‑TTTTTAAATAATAAAATTTATATTTTTAAAAATA‑‑TATTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                                                        <  1:5020771/123‑1 (MQ=255)
                                        tattaacaaaaaaaaactaTTATCTTTTTATTTAATTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGTAATTGTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTa                                                                                       <  2:3883221/116‑1 (MQ=255)
                                        tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATTTAAATATATATTTTatt                                                                                         >  2:873916/1‑126 (MQ=255)
                                        tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATTATTTAAATATAAAtttttatt                                                                                        >  1:3312769/1‑124 (MQ=255)
                                           taataaAATAAAAATATTATATTTTTTTATATTTTTTTTAAATTAAAA‑TTTTTAAAAAATTAATTTTAACTTTTAAAAAAAAGC‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAt                                                                                      <  1:3066051/128‑1 (MQ=255)
                                            aataaataaaaaataTTTTTTTTTTTTAATTTTTTTGATTTTGAAAT‑TTTTTAAAAAATTAATTTTACATTGT‑AAAAATA‑CCCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAttt                                                                                    <  1:540249/120‑1 (MQ=255)
                                            aaGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTataaaa                                                                                      >  1:2089518/1‑123 (MQ=255)
                                            aaGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTAAAT‑ATTAAAATATAAAtttaatttatt                                                                                     >  2:375164/1‑120 (MQ=255)
                                             attaaataaaaataTTATTTTTTTATATATTTTTTTTATTTTATAA‑TTTTTAAAAAATTAATTTTTAATTTTAAAAAATATC‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAttt                                                                                    <  1:3655455/122‑1 (MQ=255)
                                              gAAAATTAAAATATTATATTTTTATAATATTTTTGTATATGCAAA‑TTTTAAAAAAAATTATTTGAACATGTAAAAAA‑ATACTTTTTTTTTTTTTTTTTTATATTGGAAATATAAATTTTAGGTAtttt                                                                                   <  2:5475505/128‑1 (MQ=255)
                                              gAAAATAAAACTATTATCATTTTATTAATTTTTTTTTTTTGCAAA‑TTTTTAAAAAATTAATTTTAAATTTTAAAAAATATC‑TTTTTTTTTTTTTTTTATATAATGGAAATATAAATTTTAGGTAtttt                                                                                   <  1:1513794/128‑1 (MQ=255)
                                                aactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATTTAAATAAAAATTTTAGGAAtttatt                                                                                 >  1:2691180/1‑125 (MQ=255)
                                                  ctaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATTAAAATATAAATTTTAGTAATTttgttt                                                                               >  2:2404751/1‑127 (MQ=255)
                                                    aaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATATTGTAAAAATAAATTTTTGtttttttttttta                                                                             >  2:2076179/1‑125 (MQ=255)
                                                       actaTTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAT‑ATTAAAATATAAATTTTTGttttttttttttgaat                                                                          >  2:668987/1‑122 (MQ=255)
                                                        atattatTTTTTTATATTTTTTTTTTATATTAAAA‑TTTTTAAAAAATTAATTTTTAATTTTAAAAAAAATC‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                                                         <  1:6466535/127‑1 (MQ=255)
                                                         tattatCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTAAAATTTAAATATAAATTTTATTTATTTTTTTTTATAATTaa                                                                      >  1:6679110/1‑127 (MQ=255)
                                                         tattatCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGATTTTTTTTTTTTTTTTTTT‑TAATGTAAATATAAATTTTAGTTATTttggtgaagattt                                                                        >  2:6311752/1‑121 (MQ=255)
                                                          attatCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGTTATTTTTTTTTATAATTa                                                                       >  1:2349279/1‑128 (MQ=255)
                                                          attatATTTTTTTATTTTTTTTTTTTATTTAAA‑TTTTTTAAAAAATAATTTTTAAATTTAAAAAAT‑‑TTCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTAt                                                                      <  2:1449204/128‑1 (MQ=255)
                                                           ttatCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCTTTTTTTTTTTTTTTTTATAAAAT‑AAAAAAAAAATTTTATTTATttttttttatataaaa                                                                      >  2:2663314/1‑118 (MQ=255)
                                                                tttttttttatTTTTTTTTTATTAAAA‑TTTTTTAAAAATTAATTTTAAATTTTAAAAAAT‑TTATTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑aaa                                                                <  1:3742147/120‑1 (MQ=255)
                                                                 ttttAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATTTAAATAAAAATTTTATTAATTTTTTTTTATAATTATAT‑aaaa                                                               >  1:1001558/1‑128 (MQ=255)
                                                                   ttAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTAAAATGAAAATATAAATTTTAGTTATTTTTTTTTATAATTATaa‑aaaaaa                                                             >  1:3190339/1‑128 (MQ=255)
                                                                     atatatTTTTTGTATTTTTAAA‑TTTTTAAAAAAATAATTTGTAATTGTAAAAATAAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATAg                                                         <  2:2887049/126‑1 (MQ=255)
                                                                       ttatTTTTTTTATATTTAAATTTTTAAAAAAATTATTTTTAAATT‑TAAAAAAT‑TTGTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATAg                                                         <  1:1965979/127‑1 (MQ=255)
                                                                          ttttttGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTTTTTTTTTGTATATTTTTAT‑AAAAAAATAGAAAg                                                     >  1:655818/1‑128 (MQ=255)
                                                                           tttttGTATATTCAAA‑TTTTTAAAAAATTAATTTTTAATTTTAAAAAAT‑‑TTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATAGAAAgg                                                    <  2:6082981/128‑1 (MQ=255)
                                                                              ttttttatTCAAA‑TTTTTAAAAAATTAATTTTTAATTTTTAAAAATA‑‑TATTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATAGAAAGGaaa                                                 <  2:1352245/123‑1 (MQ=255)
                                                                              ttGTTTATTGAAA‑TTTTTAAAAAATTAATTTTAAATTTTTAAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATAGAAAGGaaa                                                 <  1:6814577/128‑1 (MQ=255)
                                                                               tGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTAAAATGGAAAAATAAATTTTATTTATTTTTTTTTCTAATTATAA‑AAAAAAATAAAAAGTaaa                                                 >  2:3442602/1‑128 (MQ=255)
                                                                                gTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTATA‑AAT‑TAAATAAAAATTTTTGTTATTTTGTTGTATAATTTTAT‑AAAAAATTAAAAAATaaaaa                                               >  2:3552116/1‑128 (MQ=255)
                                                                                gTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTATTTTTTTTTATAATTATAT‑AAAAAAATAAAAATAaaaaaa                                              >  2:4169597/1‑128 (MQ=255)
                                                                                   tatTCAAA‑TTTTTAAAAAATTAATTTTTAATTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATAGAAAGGAAAAAata                                            <  1:5206506/128‑1 (MQ=255)
                                                                                    attcAAA‑TTTTTTAAAAATTTATTTTAAATTTTTAAAAATTTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATAGAAAGGAAAAAat                                             <  2:333706/125‑1 (MQ=255)
                                                                                      gCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTTTTTTGTTGTATAAGTTAAA‑AAAAAATTAAAAAGTaaaaaaattt                                          >  2:2303938/1‑124 (MQ=255)
                                                                                       taaa‑TTTTTTAAAAATTAATTTTATTTTTTAATAAATA‑TTTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATAGAAAGGAAAAAATATTg                                         <  1:4617782/127‑1 (MQ=255)
                                                                                       cAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTTTTTTGTTGTGAAATTATCT‑AAAAAAATAAAAAGGAAAAAATAtt                                          >  2:737931/1‑128 (MQ=255)
                                                                                       cAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAAAAAGTAAAAATAATTTTTATTTATTTTTTTGTAAAAATTTAT‑AAAAAAAAAAAAAGTAAAAAAAAtt                                          >  2:5556998/1‑128 (MQ=255)
                                                                                         aa‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTTGGTTTTTTTTTGTATAAGTATAA‑AAAAAAATAGAATGGAAAAAATATtttt                                       >  2:4017439/1‑128 (MQ=255)
                                                                                          a‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTT‑TAATGTAAAATTAAATTTTTTTTTTTTTTTTGAATAAGTTAAT‑AAAAAAATAAAAAGTAAAAAAAATttttt                                      >  2:502726/1‑127 (MQ=255)
                                                                                            ttttAAAA‑AATTATTTTTAATTTTTTAAAAAT‑‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATAGAAAGGAAAAAATATTGTTAAAAg                                  <  2:1017078/128‑1 (MQ=255)
                                                                                               ttaaaaaaTTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTATATA‑‑GGAAAAATAAATTTTAGATAATTTGTGTTCATATTATCA‑TAAAAAATATAATGGAAAAAATATTTTTaaaatt                                 >  2:4302691/3‑126 (MQ=255)
                                                                                                taaaaaaTTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTAAAATTAAATAATAAATATATTTTTTTTTGTTTTATAAGTATAT‑AAAAAAAAAAAAAAGAAAATAAATTTTTaaattt                                 >  1:1658675/2‑125 (MQ=255)
                                                                                                taaaaaaTTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTATTTTTTTTTATAATTATAT‑AAAAAAATATAAAGTAAAAATTATTTTTAAAATTaa                               >  1:2460325/2‑128 (MQ=255)
                                                                                                    aaatttattTTAAATTGTTAAAAATA‑TTTTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGGTATTTTGTTTTCTAAGTATCT‑AAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATa                             <  1:4366395/127‑1 (MQ=255)
                                                                                                    aaattaattTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATTGAAATAAAAATTTTAGTTTTTTTTTTTTATATTTAAAT‑AAAAAAATATAAATTAAAAAATATTTTAAAAATTAATa                             >  1:3848758/2‑128 (MQ=255)
                                                                                                     aattaattTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATAA‑AAAAAAAAAGAAACGAAAAAAAATTTTTAAAAGTaaaaa                            >  2:6468235/1‑125 (MQ=255)
                                                                                                     aattaattTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTAAAATTTAAAAAAAAATTTTAGTTATTTTTTTTAATAATTAAAT‑AAAAAAATAAAAAGTAAAAAAAATTTTTaaaaaaaaaaa                            >  2:5989507/1‑121 (MQ=255)
                                                                                                     aattaattTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAATTTTTAGGTATTTTGTTGTGTATGTTTCT‑TAAAAAATATAAAGATAAAAATATTTTTAAAGGTAATACt                           >  1:2583080/1‑128 (MQ=255)
                                                                                                     aattaattTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAAAGAATAAAAAAATTTAAGTTATTTTTTTTTAAAATTATAA‑AAAAAAATATAAAGGAAAAAATATTTTTAAaataaataat                           >  1:4172381/1‑126 (MQ=255)
                                                                                                      attaattTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTTTTTTGTTGTATAAGTATAT‑AAAAAAATAGAAATTAAAAAATATTGTTAAATTTAATaat                           >  1:611131/1‑126 (MQ=255)
                                                                                                      attaattTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAATTATAAATTTTATGTATTTTGTTGTCTAAGTATCT‑AAAAAAATAGAAAGAAAAAAATATTTTTAAAAGTAataata                          >  2:3027293/1‑128 (MQ=255)
                                                                                                       ttaattTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGTTATTTTGTTGTGAAAGTATCT‑AAAAAAAAAGAAATTAAAAAATTTTTATAAAATTAATaat                           >  1:1121113/1‑126 (MQ=255)
                                                                                                       ttaattTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAT‑‑TGGAAATTTAATTTTTATGTTTTTTGTTGTATATGTATCT‑AAAAAAATAGAAAGTAAAAAATTTTGTTAAATGTAATACTaa                         >  1:732571/1‑128 (MQ=255)
                                                                                                       ttaattTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGAAAATATAAATTTTAGGTATTTTTTTGTAAAATTATAA‑AAAAAAAAATAAATGAAAAAAAATTTTTaaaataaaaaaaaa                         >  2:681380/1‑118 (MQ=255)
                                                                                                        taattTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATATTGGAAATATAAATTTTAGTTTTTTTGTTGTGTATGTTTTT‑AAAAAAATAGAATTGAAAAATAATTTTTaaatttattaata                          >  1:3547842/1‑118 (MQ=255)
                                                                                                        taattTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATTAAAATATAAATTTTAATTATTTTTTTTGATAATTATAT‑AAAAAAAAAAAAAGAAAAAAATATTTTTAAAATTAATaaaaa                         >  2:3181598/1‑124 (MQ=255)
                                                                                                         tattttaaattttaaAAAATATC‑TTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTaaa                        <  2:1190493/127‑1 (MQ=255)
                                                                                                         aattTGTACTTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTaaaa                       <  2:5677860/128‑1 (MQ=255)
                                                                                                         aattTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTATTTTGTTGTATAAGTTTCTAAAAAAAATAGAAATAAAAAAATATTTTTAAAATTTATACTaaaa                       >  1:4887072/1‑128 (MQ=255)
                                                                                                               aaattGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTAttt                 <  1:6007984/125‑1 (MQ=255)
                                                                                                                aCTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTA‑AATGGAAATATAAATTTTATGTATTTTGTTTTCTAATTAAAT‑TAAAAAATATAAAGGAAAAAATATTTTTAAAATTAATAATAAAATAAttt                 >  1:1843596/1‑128 (MQ=255)
                                                                                                                aCTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATGAAAATATAAATTTTAGTTTTTTTGTTGGAAAAGTATAA‑AAAAAAATAAAAAGAAAAAAATTTTTTAAAAATTAATAAAAAAATTAttt                 >  1:5116878/1‑128 (MQ=255)
                                                                                                                 cTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTATAAGTATAT‑AAAAAAATAGAAAGGAAAAAATTTTTTTAAAAGTAATACAAAAATTATTTaa               >  1:175338/1‑128 (MQ=255)
                                                                                                                  ttGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTGAAAAAAAAATTTTAGTTATTTTGTTTTATATTAATAT‑AAAAAAAAAGAAAGTAAAAATATTTTTTAAAATTAATAAAAAATTTAATTa                >  1:4726529/1‑128 (MQ=255)
                                                                                                                  ttGTAAAAAATAGCTTTTTTTTTTTTTTTTTTT‑TAATTGAAATATAAATTTTTTGTTTTTTTTTTTATAATTATAT‑AAAAAATTATAAATGAAAAAATATTTTTAAAATTAAAAATAAAATTATTTaa               >  1:6352382/1‑128 (MQ=255)
                                                                                                                   tGTTAAAAATAGTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTaa               <  2:5115183/128‑1 (MQ=255)
                                                                                                                    gTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAAAATGAAAATATAAATTTTATTTATTTTTTTTTATAATTATAT‑AAAAAAATATAAATTAAAAAATATTTTTAAAATTAATAAAAAAATAATTAAAt              >  2:2488621/1‑128 (MQ=255)
                                                                                                                    gTAAAAAATAGCTTTTTTTTTTTTTTTTTTT‑TAATGGAAAATAAAATTTTTTGTATTTTTTTGTATAATTATAT‑AAAAAAATATAAAATAAAAAATATTTTTAAAATAATTAAAAAATTTATTTAAtt             >  2:2203169/1‑127 (MQ=255)
                                                                                                                    gTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATGGAAATTTAAATTTTAGTTATTTTTTTTTATAATTATAT‑AAAAAAATAAAAAGGAAAAAATATTTTTAAAATTAATAATAAAATTATTTAAtt             >  1:5717027/1‑127 (MQ=255)
                                                                                                                    gTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTTGGTATTTTTTTGTATATGTATCT‑AAAAAAATAGAAATTAAAAAATATTTTAAAAAATAATACTAAAATATTTTAATg             >  1:5325990/1‑128 (MQ=255)
                                                                                                                         aaaTAGCTTTTTTTTTTTTTTTTTTTATAATTTAAAAATAAAATTTATTTATTTTTTTTTAAAATTAAAT‑AAAAAAAAATAAATAAAAAAAAATTTTTAAAAAAAAAAAAAAAATTAATTAATTttaa         >  2:1717495/1‑128 (MQ=255)
                                                                                                                         aaaTAGC‑TTTTTTTTTTTTTTTTTTAAAATGTAAATAAAAATTTTAGGTATTTTTTTTTATAAGAAACA‑AAAAAAATATAAAGTAAAAAATATTTTTAAAAGAAAAACTAATATTATTAAAttaaaat        >  2:6207310/1‑122 (MQ=255)
                                                                                                                           ttttttttttttttttttttttttATAATTGAAATATAAATTTTAGTTATTTTGTTGTCTAAGTATCT‑AAAAAAATAGAAATGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGttaatt       <  1:295819/123‑1 (MQ=255)
                                                                                                                           aattttttttttttttttttttttATAATGGAAATATTAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGttaatt       <  2:1976744/123‑1 (MQ=255)
                                                                                                                                tttttttttttttttttttATAATGGAAATATAAATTTTAGTTATTTTGTTTTATAAGTATCT‑AAAAAAATAGAAAGTAAAAAATACTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAAc  <  1:4077129/128‑1 (MQ=255)
                                                                                                                                |                                                                                                                                
ATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAAC  >  CP016816/165123‑165377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑