Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 87,917 (T)15→14 intergenic (+44/‑816) JCVSYN2_00345 → / → JCVSYN2_00350 hypothetical protein/50S ribosomal protein L31

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP01681687,9030T.80.9% 5317.7 / inf 2351intergenic (+30/‑830)JCVSYN2_00345/JCVSYN2_00350hypothetical protein/50S ribosomal protein L31
Reads supporting (aligned to +/- strand):  ref base T (218/203);  new base . (891/897);  total (1136/1215)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2768 total aligned reads displayed.

TGTGTAACAAAAGTGGTTATTGAGTTGCAAGTAAAGTTCAAATTGATAATAAAATTGATATTCATGGAAAGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTATTTACCTTTCTTTAGGTAACTAATTAATTACCTAATATTATTTTAG  >  CP016816/87776‑88030
                                                                                                                                 |                                                                                                                               
 gtgtAACAAAAGTGGTTATTGAGTTGCAAGTAAAGTTCAAATTGATAATAAAATTGATATTCATGGAAAGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCAta                                                                                                                                >  2:2639809/1‑127 (MQ=255)
    tAACAAAAGTGGTTATTGAGTTGCAAGTAAAGTTCAAATTGATAATAAAATTGATATTCATGGAAAGTCAAATGCATATTTAGAGTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCAttttt                                                                                                                             >  2:638111/1‑128 (MQ=255)
      actataggggTTTTTGAGTTGCAATTAAAGTTTAAATTGATAATAAAATTGATATTCATGGAAATTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCAttttttt                                                                                                                           <  2:672257/120‑1 (MQ=255)
      aCAAAAGTGGTTATTGAGTTGCAAGTAAAGTTCAAATTGATAATAAAATTGATATTCATGGAAAGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCAttttttt                                                                                                                           >  2:2214209/1‑128 (MQ=255)
       aaaaaTTGGTTATTGAGTTGCAATTAAATTTAAAATTTATAATAAAATTTATATTCATGGAAATTAAAATGAATATTTATATTTTTAATAGCTGATTTAAATGGTTTATTAAACAATCCAtttttttt                                                                                                                          <  1:6750241/127‑1 (MQ=255)
        aaaaGTGGTTATTGAGTTGCAAGTAAAGTTCAAATTGATAATAAAATTGATATTCATGGAAAGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCAttttttttt                                                                                                                         >  2:2867394/1‑128 (MQ=255)
        aaaaGTGGTTATTGAGTTGCAAGTAAAGTTCAAATTGATAATAAAATTGATATTCATGGAAAGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCAttttttttt                                                                                                                         >  1:299589/1‑128 (MQ=255)
          aaGTGGTTATTGAGTTGCAAGTAAAGTTCAAATTGATAATAAAATTGATATTCATGGAAAGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCAttttttttttt                                                                                                                       >  1:6069750/1‑128 (MQ=255)
           attGGTTATTTAGGTGCAATTAAATTTCAAATTTATAATAAAATTGATATAAATGGAAATTCAAATTAATATTTATATTTTGAAAAGCTGATTTAAATGGATTATTAAACAATCCAtttttttttttt                                                                                                                      <  1:5254258/126‑1 (MQ=255)
              ggTTATTGAGTTGCAAGTAAAGTTCAAATTGATAATAAAATTGATATTCATGGAAAGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCAtttttttttttttaa                                                                                                                   >  2:5512688/1‑126 (MQ=255)
                   ttGAGTTGCAAGTAAAGTTCAAATTGATAATAAAATTGATATTCATGGAAAGTCAAATGATTATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTtataaa                                                                                                              >  2:23030/1‑124 (MQ=255)
                   ttGAGTTGCAAGTAAAGTTCAAATTGATAATAAAATTGATATTCATGGAAAGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTAATaaa                                                                                                             >  2:309762/1‑128 (MQ=255)
                    tGAGTTGCAAGTAAAGTTCAAATTGATAATAAAATTGATATTCATGGAAAGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTtaaataaa                                                                                                             >  2:6244535/1‑128 (MQ=255)
                     tcgTTGAAATTAAAGTTCAAATTGATAATAAAATTGATATTCATGGAAAGTCAAAAGAATATTTATTTTTTTAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTTAATaaaa                                                                                                            <  1:2667787/126‑1 (MQ=255)
                      aGTTGCAAGTAAAGTTCAAATTGATAATAAAATTGATATTCATGGAAAGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTAATAAAAAg                                                                                                          >  1:3904357/1‑128 (MQ=255)
                       gTTGCAATTAAAGTTAAAATTTTAAATAAAATTGATATTAATTGAAAGTAAAATTAATTTTTATTTTTTTAATATCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTAATAAAAAGa                                                                                                         <  1:6413712/128‑1 (MQ=255)
                        ttGAAATTAAAGTTCAAATTGATAATAAAATTTATTTTCATGGAAAGTAAAATTATTATTTAGATTTTGAATAGCTTATTTAAATGGTTTATTAAACAATCCATTTTTTTTTTTTTTAATAAAAAGAc                                                                                                        <  1:1315249/128‑1 (MQ=255)
                           catttataGTTCACATTGATAATAAAATTTATATAGATGGAAATTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAAACCATTTTTTTTTTTTTTAATAAAAAGACTca                                                                                                     <  1:6483867/121‑1 (MQ=255)
                           cAAGTAAAGTTCAAATTGATAATAAAATTGATATTCATGGAAAGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTAATAAAAATACtaa                                                                                                     >  1:3190436/1‑126 (MQ=255)
                           cAAGTAAAGTTCAAATTGATAATAAAATTGATATTCATGGAAAGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCA‑‑TTTTTTTTTTTTTAATAAAAAGACTcac                                                                                                    <  1:1131386/128‑1 (MQ=255)
                           cAAGTAAAGTTAAAAATGAAAAATAAAATTTTTATCATGGAAATTTAAATTAATATTTATCTTTTTAATACCTGATTTAAATGGATTATTAAACAAACCA‑‑TTTTTTTTTTTTTAATAAAAAGACTcac                                                                                                    <  2:4292447/128‑1 (MQ=255)
                           cAAGAAAAGTTCATATTGATAATAAAATTTCTATACAAGCAAAGTAAAATGAATATTTAGATTTTGAATAGCTGAATTAAATTGATTATTAAACAATCCATTTTTTTTTTTTTTAATAAAAAGACTca                                                                                                     <  1:2862709/128‑1 (MQ=255)
                             aGTAAAGTTCTATTTGAAAATAAAATTGAAATTCATGGAAAGTCAAATGAATATTTTGATTTTTAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTAATAAAAAGACTcact                                                                                                   <  1:2232035/128‑2 (MQ=255)
                              ttAAAGTTCAAATTTTATATAAAAATGATATTAATGTTAATTCAAATTAATATTTAGTATTTTAATACCTGATTTAAATCGAATATTAAACAATCCATTTTTTTTTTTTTTTAATAAAAAGACTcact                                                                                                   <  1:4593201/127‑2 (MQ=255)
                               tAAATTTTAAATTGATAATAAAAATTTAATTCATTGTAACTCAAATTAATATTTATATTTTTTATATCTTTTTTAAATGGATTTTTAAACAAACCATTTTTTTTTTTTTTTTAATAAAAAGACTcact                                                                                                   <  1:470927/128‑2 (MQ=255)
                               tAAAGTTCAAATTGATAATAAAATTGATATTCATGGAAAGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTTAATAAAAAAACACACAt                                                                                                  >  2:4902848/1‑128 (MQ=255)
                                 aaGTTCAAATTTATAATAAAATTTATATTCAATGAAAGTAAAATTAATATTTAGTTTTTTAAAATCTTATTAAAATGGATTTTTTAATAAACCATTTTTTTTTTTTTTAATAAAAAGACTCACATAAg                                                                                               <  1:3821107/128‑1 (MQ=255)
                                    ttCAAATTGATAATAAAATTGATATTCATGGAAAGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATTGATTATTAAACAATCCATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGa                                                                                            <  1:5130649/128‑1 (MQ=255)
                                        aatttgcaaaataaaTTGTAATTTCTGGCAACTCAAATCAAAATTTATCTTTTGAAAAGCCGATTTAAATGGAATATTAAACAATCCATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTc                                                                                         <  1:1383845/116‑1 (MQ=255)
                                              ataataAAATTGATATTCATGGAAAGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTAATAAAAATAATCACATAATTGAGTAAATTGc                                                                                   >  2:2995441/1‑128 (MQ=255)
                                               taataaAATTGATATTCATGGAAAGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTAATAAAAATAATCACATAATTGATACAATTTCg                                                                                  >  2:588995/1‑128 (MQ=255)
                                                 ataaAATTGATATTCATGGAAAGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGat                                                                                <  1:1838882/128‑1 (MQ=255)
                                                    aaaTTGATATTCATGGAAAGTCAAATGAATTTTTAGATATTGAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGattat                                                                             <  1:4439802/128‑1 (MQ=255)
                                                    aaaTTGATATTCATGGAAAGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTTAATAAAAAGACTAACATAAGTGATTAAAtatctatta                                                                              >  2:5153420/1‑120 (MQ=255)
                                                      aTTGATATTCATGGAAAGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTAATAAAAAGACTCACAt                                                                                                  <  1:419157/105‑1 (MQ=255)
                                                      aTTGATATTCATGGAAAGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCAATTTTTTTTTTTTTTATTAAAAAAAAAAACAAAAGTTATTCAATATAGATTAttt                                                                           >  1:1598710/1‑128 (MQ=255)
                                                        tGATATTCATTTAAAGTAAAATTAATATATTGATTTTTAATATCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATtta                                                                          <  2:6500176/128‑1 (MQ=255)
                                                        tGATATTCATGGAAAGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATttaa                                                                         >  1:5825467/1‑128 (MQ=255)
                                                         gATATTCATGGAAAGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTAATAAAAAAAATCACATAATTTAGTCAATTGCGATTATttaat                                                                        >  1:4066513/1‑128 (MQ=255)
                                                           tatTCATGGAAAGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCA‑‑TTTTTTTTTTTTTAATAAAAAGAATCAAATAAGTGAGACAATTTCGATTTTttaattat                                                                     >  2:1345887/1‑128 (MQ=255)
                                                             ttCATTGAAATTAAAATTAATATTTATATTTTGAATATCTTATTTAAATGGATTATTAAAAAATAAATTTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATttaattat                                                                     <  1:1006291/128‑1 (MQ=255)
                                                                aTGGAAAGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTAATAAAAATAATACAATAATTTATTAAATTTCTATTATttaattattaat                                                                 >  1:3718834/1‑128 (MQ=255)
                                                                 tGGTAATTCAAATTAATATTTAGATTTTTAATAGCTGATTTAAATTGATTATTAAACAAACCATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATttaattattaatt                                                                <  2:903149/128‑1 (MQ=255)
                                                                 tGGAAAGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTTTAAAAAAAAGAAACAAATATTTGTGAAATTTGAGAATTTtttattattaa                                                                  >  2:760406/1‑128 (MQ=255)
                                                                  tgAAAGTCAAATTAATATTTAGATTTTGAATAGATTATTTAAATGGATTATTAAACAATACATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATttaattattaatta                                                               <  1:497205/127‑1 (MQ=255)
                                                                   gAAAGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTAATAAAAATACACAAATAAGTTAGTAAATTTCAATTATTtaattaataaatat                                                              >  2:1056204/1‑128 (MQ=255)
                                                                    aaaGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTAATAAAAAGACTAACAAAAGTGAGTAAATTGCGATTATttattttttaattaat                                                             >  1:3424024/1‑126 (MQ=255)
                                                                     aattCAAATTATTATTTAGAATTTGAATAGCTTATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTc                                                            <  1:3330998/125‑1 (MQ=255)
                                                                     aatgaaaaTTATTATTTATTTTTTGAATATCTTTTTTAAATTGATTATTAAACAATTCATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCTATTATTTAATTATTAATTATTc                                                            <  2:4159006/123‑1 (MQ=255)
                                                                      aGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTAATAAAAAGAATCACATAAGTGAGTCAATTGAAAATATTTaattattaattattat                                                           >  2:1803757/1‑126 (MQ=255)
                                                                        tCAAATTAATTTTTTGTTTTTGTATAGCTGTTTTAAATGGTTTATTAAAAAATCCATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCttt                                                         <  2:5261329/128‑1 (MQ=255)
                                                                          aaaTGAATATTTTTTATTTTAATATGTGATTTAAATTGTTTATTTAAAAATACATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTtga                                                       <  1:2327634/128‑1 (MQ=255)
                                                                            aTGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATGCATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTtgatg                                                     <  1:309762/128‑1 (MQ=255)
                                                                            aTGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTAATAAAAATACTAAAAAAATTAATAAAATAGATATAATTTAATTAATAATAattattaaata                                                     >  1:950699/1‑123 (MQ=255)
                                                                              gatattttATATTTTTAAAATCTTTTTTAAATTGATAAATAAAAAATAGATTTTTTTTTTTTTTAATAAAAAGAATCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGaa                                                   <  2:4490608/123‑1 (MQ=255)
                                                                               aaTATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCA‑‑TTTTTTTTTTTTTAATAAAAATACTCACATAAGTTAGTCAATTGCCATTATTTAATTATTAATTATTCTTTGATAAACt                                                 >  2:4639571/1‑128 (MQ=255)
                                                                               aaTATATAGATTTTTTATAGCTGTTTTAAATGGATTATTAAAAAATCCATTTTTTTTTTTTTTAATAAAAAGACTCAAATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAAc                                                  <  2:5775258/128‑1 (MQ=255)
                                                                                atatTTAGATTATTAATATCTTATTTAAATAGATTATTAAAAAATACATTTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAAc                                                  <  1:6301423/128‑1 (MQ=255)
                                                                                 tatTTTGTTTTTTTATTGATGATTTAATTGTATTTTTAAACAATCAATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTa                                                <  1:867674/128‑1 (MQ=255)
                                                                                 tatTTATATTTTTAATAGATTATTTAAATTGATTATTAAAAAATACATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTa                                                <  1:4843715/128‑1 (MQ=255)
                                                                                 tatTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCA‑‑TTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTAt                                               <  1:6857146/128‑1 (MQ=255)
                                                                                   tttATATTTTTAATAGATTTTTTAAATGTATTATTAAAAAATCAATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTAtt                                              <  1:5938267/128‑1 (MQ=255)
                                                                                      aGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTAAATAAAAAAAAAAAAAAAATTGAAACAATTTCTATTATTAAATTATAAATTATTATTTTATAAAAAATTTAc                                           >  2:5027252/1‑128 (MQ=255)
                                                                                      aGATTTTGAATAGCTGATTTAAATGGATTAATAAACAATCAATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTATTTAc                                           <  2:6843213/128‑1 (MQ=255)
                                                                                       taTTTTGAATATCTGATTTAAATTTTTTATTAAACAATCCATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTATTTAcc                                          <  1:5403899/127‑1 (MQ=255)
                                                                                        aTTTTTAATATCTTATTTAATTGGATTATTAAAAAATCAA‑‑TTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTATTTACctt                                        <  1:3593872/128‑1 (MQ=255)
                                                                                        aTTTTGAATAGCTGATTTAAATGTATTATTAAACAATCCATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTATTTACct                                         <  1:5686835/128‑1 (MQ=255)
                                                                                        aTTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTAAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTATTTACct                                         >  2:2510767/1‑128 (MQ=255)
                                                                                         ttttGAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTTAATAAAAAGACTCCCATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTTTTTAAct                                         >  2:3749971/1‑128 (MQ=255)
                                                                                          tttGAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTATTTACcttt                                       <  1:1721729/128‑1 (MQ=255)
                                                                                           tttaaatgcttttttAAATGGAATTTTAAAAAAATAATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTATTTACctttc                                      <  2:2078000/116‑1 (MQ=255)
                                                                                            ttttttttttttttAAATTTTTTATTAAAAAATTATTTTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTAAATTGATATTATTTAATTATTAATTATTATTTGATTAACTATTTACctttc                                      <  2:15538/117‑1 (MQ=255)
                                                                                             gAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTTAATAAAAATACAAAAATAATTTATTAAATTGATATTATTTATTTATTAATTATTATTTTATAAAATATTTaaatttat                                     >  1:2777613/1‑121 (MQ=255)
                                                                                               atatCTTATTTAAATTGTTTATTTAACAATTAATTTTTTTTTTTTTTTAATATAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTATTTACctttcttt                                   <  2:2411131/128‑1 (MQ=255)
                                                                                                ttttttttttAAATTTTTTTTTAAAAAATTCTTTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTATTTACCTTTCTTTAg                                 <  2:6611375/121‑1 (MQ=255)
                                                                                                tatcttaatttaaTTGATTATTAAACAATCCATTTTTTTTTTATTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTATTTACCTTTCTTTAg                                 <  2:1957811/117‑1 (MQ=255)
                                                                                                 atcTGATATAAATTGAATAATAAACAATCCA‑‑TTTTTTTTTATTTAATAACAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTATTTACCTTTCTTTAGGt                               <  2:3360774/126‑1 (MQ=255)
                                                                                                 atatGATTTAAATGTATTAATAAACAATTCATTTTTTTTTTTTTTAATAAAAAGACTCATATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTATTTACCTTTCTTTAgg                                <  1:150691/125‑1 (MQ=255)
                                                                                                  gCTGATTTAAATGGATTATTAAACAATAAATTTTTTTTTTTTTTAATAAAAATAATAAAATAATTTATACAATTACAATTTTTTAATTATTAATTATAATTTTATGAAATTTTTACATTTATTTAgta                               >  2:1454015/1‑126 (MQ=255)
                                                                                                   gttaTTTAATTGGTTTATTAAACAATCCATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTATTTACCTTTCTTTAGGTa                              <  1:619498/125‑1 (MQ=255)
                                                                                                    tGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTATTTACCTTTCTTTAGGTaa                             >  2:3210431/1‑128 (MQ=255)
                                                                                                      aTTTAAATGGATTATTAAACAATCCA‑‑TTTTTTTTTTTTTAATAAAAAGAATCACAAAAGTCAGTCAATTGCGATTATTTAATTATAAATTATTATTTGATGAAATATTTACCTTTTTTTAGTTAACta                          >  2:1664846/1‑128 (MQ=255)
                                                                                                       tttAAATGGATTATTAAACAATCCATTTTTTTATTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTATTTACCTTTCTTTAGGTAACta                          >  1:6611285/1‑128 (MQ=255)
                                                                                                        ttAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTAATAAAAAGACACACATAATTGAGTCAATTGCGATTATTTAATTTTTAATTATTCTTTGATTAACTATTTACATTTATTTAGTTAACtaa                         >  2:4002978/1‑128 (MQ=255)
                                                                                                         ttaattgtttattaAATAATTCATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTATTTACCTTTCTTTAGGTAACtaat                        <  2:5425107/120‑1 (MQ=255)
                                                                                                             ttttttattaAACAATACATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTATTTACCTTTCTTTAGGTAACtaattaat                    <  2:3876050/124‑1 (MQ=255)
                                                                                                               gATTATTAAACAATCCATTTTTTTTTTTTTTTATTAAAAAAACACACATAAGTGAGTCAATGGCGTTTATTTAATTATTATTTATTATTTGTTAAAATTTTTCCATTAGTTTAGTTAACTatttaatt                   >  1:2143856/1‑128 (MQ=255)
                                                                                                               gATTATTAAACAATCCA‑‑TTTTTTTTTTTTTAAAAAAAAGACAAAAATAAGTGAGTAAATTGCGATTTTTTAATTATTAATTAATATTTTAATAACTATTTACATTTCTTTATGTAACTATTTAATTAc                 >  2:1192123/1‑128 (MQ=255)
                                                                                                                attattATACAATTCATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTAGATGAACTATTTACCTTTCTTTAGGTAACTAATTAATTAc                 <  1:2014256/128‑1 (MQ=255)
                                                                                                                attattAAACAATCCATTTTTTTTTTTTTTTAATAAAAAAACAAACAAAAGTAATAAAATTGCCATAATTTAATTATTAATTATTATTTAATAAAATATTTACATTACTTTAGTTAACtaattaataa                  >  2:3649746/1‑126 (MQ=255)
                                                                                                                 ttattaAACAATCCATTTTTTTTTTTTTTTAAAAAAAATACTCAAATAATTGATTAAATTGCTATTATTAAATTATTTATAATAATTTAATTAAATATTTAACTTACTTAATTTAAATAataaattaa                 >  2:3512909/1‑127 (MQ=255)
                                                                                                                   attaAACAATCCATTTTTTTTTTTTTAAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTATTTACCTTTCTTTAGGTAACTAATTAATTACCTa              <  2:4404014/128‑1 (MQ=255)
                                                                                                                    ttaAATAATCAATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTATTTACCTTTCTTTAGGTAACTAATTAATTACCTaa             <  1:4343617/128‑1 (MQ=255)
                                                                                                                    ttaAACAATCCATTTTTTTTTTTTTTAATAAAAAGCCTCCCATAAGTGATGCAATTCCGATTATTTAATTTTTAATTATTATTTGAGTAAATATTTACATTAGTTTAGGTAAAAAATTAATAACATat             >  2:6062276/1‑127 (MQ=255)
                                                                                                                    ttaAACAATCCATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTATTTACCTTTCTTTAGGTAACTAATTAATTAACTaa             >  1:251772/1‑128 (MQ=255)
                                                                                                                     taAACAATCCATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTATTTACCTTTCTTTAGGTAACTAATTAATTACCTAat            <  1:2426089/128‑1 (MQ=255)
                                                                                                                     taAACAATCCATTTTTTTTTTTTTTAAAAAAAAAACTAAAATAATTGATAAAATTGCTTTTATTTAATTTTTAAATATTGTTTAATTAAATATTTAACTTTCTATAGTTAAAAAATTAATTAccatat            >  2:2749610/1‑124 (MQ=255)
                                                                                                                        aCAATCCATTTTTTTTTTTTTTTAATAAAAAGAATCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTATTTGATGAACTATTTACATTTATTTAGTTAAATAATTAATTACCTAatat          >  1:5562920/1‑128 (MQ=255)
                                                                                                                          aaTCCATTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTATTTACCTTTCTTTAGGTAACTAATTAATTACCTAAtattat       <  2:5646999/128‑1 (MQ=255)
                                                                                                                               aTTTTTTTTTTTTTTTTATAAAAAGTCTCAAATAAGTGTGTCAATTGCGATTATTTTATTATTAATTATTCTTTGATGAAATATTTACCTTTCTTTAGGTAACTAATTAATTACCTAATATTATTTTa   <  2:4770543/128‑1 (MQ=255)
                                                                                                                                 |                                                                                                                               
TGTGTAACAAAAGTGGTTATTGAGTTGCAAGTAAAGTTCAAATTGATAATAAAATTGATATTCATGGAAAGTCAAATGAATATTTAGATTTTGAATAGCTGATTTAAATGGATTATTAAACAATCCATTTTTTTTTTTTTTTAATAAAAAGACTCACATAAGTGAGTCAATTGCGATTATTTAATTATTAATTATTCTTTGATGAACTATTTACCTTTCTTTAGGTAACTAATTAATTACCTAATATTATTTTAG  >  CP016816/87776‑88030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: