Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816478,6820CT66.8% 1004.2 / inf 900E320K (GAA→AAA) JCVSYN2_02210DNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base C (125/171);  new base T (284/311);  total (413/487)
Fisher's exact test for biased strand distribution p-value = 1.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.44e-01

Only 100 of 912 total aligned reads displayed.

TACCTTGAACTGGTTTTGGTTTTCTTTCATTATCAAATAGAGCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCA  >  CP016816/478556‑478809
                                                                                                                              |                                                                                                                               
tACCTTGAACTGGTTTTGGTTTTCTTTCATTATCAAATAGAGCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCa                                                                                                                                <  2:184659/128‑1 (MQ=255)
 aCCTTGAACTGGTTTTGGTTTTCTTTCATTATCAAATAGAGCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCAt                                                                                                                               <  2:1211372/128‑1 (MQ=255)
  ccTTGAACTGGTTTTGGTTTTCTTTCATTATCAAATAGAGCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCAtt                                                                                                                              <  1:1447223/128‑1 (MQ=255)
    ttGATCTGGTTTTGGTTTTCTTTCATTATCAAATAGAGCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTc                                                                                                                            <  2:1532640/128‑1 (MQ=255)
    ttGAACTTGTTTTGGTTTTCTTTCATTATCAAATAGAGCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTc                                                                                                                            <  1:1781569/128‑1 (MQ=255)
     tGAACTGGTTTAGGTTTTCTTTCATTATCAACTAGAGCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCt                                                                                                                           <  2:453576/128‑1 (MQ=255)
      gAACTGGTTTTGGTTTTCTTTCATTATCAAATAGAGCATCAACAGCTTCTTGTAACATACGTTTTTCTTTATTTACAATAATTGATGATGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCta                                                                                                                          >  2:1678541/1‑128 (MQ=255)
      gAACTGGTTTTGGTTTTCTTTCATTATCAAATAGAGCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCta                                                                                                                          >  1:749520/1‑128 (MQ=255)
      gAACTGGTTTTGGTTTTCTTTCATTATCAAATAGAGCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTAACAATAAATGATGGAGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCta                                                                                                                          >  2:801976/1‑128 (MQ=255)
       aaCTGGTTTTGGTTTTCTTTCATTATCAAATAGAGCATCAACAGCTTCTTGTATCATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCtaa                                                                                                                         <  2:1658156/128‑1 (MQ=255)
         cTGGTTTTGGTTTTCTTTCATTATCAAATAGAGCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCtaata                                                                                                                       >  2:218937/1‑128 (MQ=255)
          tggttttggtttTCTTTCATTATCAAATAGAGCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCtaataa                                                                                                                      >  2:1711389/1‑128 (MQ=255)
           ggttttggttttCTTTCATTATCAAATAGAGCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTaataat                                                                                                                     >  2:649732/1‑128 (MQ=255)
              tttggttttCTTTCATTATCAAATAGAGCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGAt                                                                                                                  <  2:739738/128‑1 (MQ=255)
                tggttttCTTTCATTATCAAATAGAGCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGAt                                                                                                                  <  2:931070/126‑1 (MQ=255)
                 ggttctcttaacttATCAAATTGAGCATCAACAGCTTCTTGTAACATACGTTTTTCATAATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGAttct                                                                                                               <  1:1912438/116‑1 (MQ=255)
                      tctttcATTATCAAATAGAGCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTACCTTTTTTTAATCTTTTAATTCTAATAATGATTCTTCtat                                                                                                          >  2:1287061/1‑128 (MQ=255)
                         ttcATTATCAAATAGAGCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGTTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCtatata                                                                                                       >  1:1061712/1‑128 (MQ=255)
                          tcATTATCAAATAGAGCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATaa                                                                                                      <  1:1974558/128‑1 (MQ=255)
                          tcATTATCAAATAGAGCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATaa                                                                                                      <  2:963384/128‑1 (MQ=255)
                           cATTATCAAATAGAGCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATaaa                                                                                                     >  2:1562044/1‑128 (MQ=255)
                           cATTATCAAATAGAGCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATaaa                                                                                                     <  2:1937335/128‑1 (MQ=255)
                            aTTATCAAATAGAGCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAAt                                                                                                    <  1:221104/128‑1 (MQ=255)
                            aTTATCAAATAGAGAATCATCAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAAt                                                                                                    <  2:572633/128‑1 (MQ=255)
                               ataaaaTAGAGCATCAAGAGCTTCTTGTAACATACGTTTTTCATTATTTACAATATTTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCAt                                                                                                 <  1:1411064/125‑1 (MQ=255)
                               aTCAAATAGAGCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCAt                                                                                                 <  2:1473821/128‑1 (MQ=255)
                                tCAAATAGAGCATCAACAGCTTCTTGTAACATACTTTTTTAATTATTTACAATAATTGATGGTGCACCCATTTATTTAACTTTTTTTAATCTTTTATTACTAATAATGATTCTTCTATATAAATCAtt                                                                                                >  2:33154/1‑128 (MQ=255)
                                tCAAATAGAGCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCAtt                                                                                                >  2:1425391/1‑128 (MQ=255)
                                  aaaTAGAGCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTaa                                                                                              >  1:97402/1‑128 (MQ=255)
                                     tAGAGCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATaaa                                                                                                     >  1:1214994/1‑118 (MQ=255)
                                      agagCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAAt                                                                                                    >  1:1026693/1‑118 (MQ=255)
                                      agagCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAAttac                                                                                          >  1:648564/1‑126 (MQ=255)
                                      agagCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTc                                                                                          <  2:196316/128‑1 (MQ=255)
                                      agagCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTc                                                                                          >  1:433161/1‑128 (MQ=255)
                                         tcATCAACAGCTTCTTGTAACATAAGTTTTTGATTATTTACAATAATAGATGGAGCACACATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGa                                                                                       <  1:1312798/127‑1 (MQ=255)
                                             cAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTAtt                                                                                                                              >  2:1165245/1‑85 (MQ=255)
                                             cAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTACTTTAACTTTTTTAAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAagta                                                                                   >  1:859514/1‑128 (MQ=255)
                                               aCAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAagtagt                                                                                 <  2:1808026/128‑1 (MQ=255)
                                                cAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAAttt                                                                                           <  2:760851/117‑1 (MQ=255)
                                                 aGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTaa                                                                               >  2:1116300/1‑128 (MQ=255)
                                                 aGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGCTTCTTCTATATAAATCATTAATTTCAGAAGTAGTaa                                                                               >  1:1636213/1‑128 (MQ=255)
                                                 aGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTaa                                                                               >  1:1356561/1‑128 (MQ=255)
                                                 aGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTaa                                                                               >  1:12787/1‑128 (MQ=255)
                                                   cttcttGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAAt                                                                             >  2:1005795/1‑128 (MQ=255)
                                                   attcttGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAAt                                                                             <  1:1543645/127‑1 (MQ=255)
                                                    ttcttGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATc                                                                            <  1:506695/128‑1 (MQ=255)
                                                     tcttGTAATATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCt                                                                           <  1:468069/128‑1 (MQ=255)
                                                     tcttGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCt                                                                           <  1:1731260/128‑1 (MQ=255)
                                                     tcttGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCt                                                                           <  1:1118878/128‑1 (MQ=255)
                                                        tGTAACATACGTTTTTCATTATTTACTATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGTTTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTacc                                                                        <  2:1911565/128‑1 (MQ=255)
                                                        tGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTaac                                                                        >  1:1232157/1‑126 (MQ=255)
                                                        tGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACACATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTacc                                                                        <  2:1043751/128‑1 (MQ=255)
                                                            aaaTACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGAttct                                                                                                               <  1:1005976/83‑1 (MQ=255)
                                                             cATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCAt                                                                   >  1:1468175/1‑128 (MQ=255)
                                                                 cGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTaa                                                               >  1:298437/1‑128 (MQ=255)
                                                                   tttttCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAAtt                                                             >  2:244088/1‑128 (MQ=255)
                                                                   tttttCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATaa                                                                                                      <  1:1664549/87‑1 (MQ=255)
                                                                   tttttCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAAtt                                                             <  2:61973/128‑1 (MQ=255)
                                                                     tttCAATATTTAAAATAAATTATTGTTCAACCATTTCTTTAACTTTTTATAATCTATCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGa                                                           <  2:534660/128‑1 (MQ=255)
                                                                         attattTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGaataa                                                       <  1:588725/128‑1 (MQ=255)
                                                                             tttACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTgg                                                   <  1:1199445/128‑1 (MQ=255)
                                                                                aCAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGAct                                                >  2:889384/1‑127 (MQ=255)
                                                                                 cAATAATTGATGGTGCACCCATTTCTCTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGa                                               >  2:694649/1‑128 (MQ=255)
                                                                                    taatTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATg                                            <  1:1940051/128‑1 (MQ=255)
                                                                                     atttGATGGTTCACCTATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATTATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGt                                           <  1:713788/126‑1 (MQ=255)
                                                                                     aatTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaata                                                        >  2:553244/1‑115 (MQ=255)
                                                                                      atTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTc                                          <  2:403083/128‑1 (MQ=255)
                                                                                      atTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTc                                          <  1:255986/128‑1 (MQ=255)
                                                                                      atTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTc                                          <  2:1879900/128‑1 (MQ=255)
                                                                                       ttGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCt                                         <  2:1232157/128‑1 (MQ=255)
                                                                                        tGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCtg                                        <  1:327815/128‑1 (MQ=255)
                                                                                        tGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtg                                        >  1:90185/1‑128 (MQ=255)
                                                                                          aTGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCtggt                                      <  1:1163166/128‑1 (MQ=255)
                                                                                           tGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCtggtg                                     <  2:511271/128‑1 (MQ=255)
                                                                                             gTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGa                                   >  1:933479/1‑128 (MQ=255)
                                                                                              tGCACACATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGaa                                  <  2:1088368/128‑1 (MQ=255)
                                                                                                cACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACAACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATa                                >  2:1346991/1‑128 (MQ=255)
                                                                                                cACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCAATAATTTCAGAAGTGGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATa                                >  2:1754051/1‑128 (MQ=255)
                                                                                                cACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATa                                >  1:1863471/1‑128 (MQ=255)
                                                                                                cACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATa                                <  2:294887/128‑1 (MQ=255)
                                                                                                cACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAAATGGACGAATGTCTGGTGGAATa                                >  1:109356/1‑128 (MQ=255)
                                                                                                   ccATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACt                             >  1:1693647/1‑128 (MQ=255)
                                                                                                      tttCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGa                          >  2:1386916/1‑128 (MQ=255)
                                                                                                      tttCTTTAACTTTTTTTAATCTTTCATATCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGa                          <  2:372691/128‑1 (MQ=255)
                                                                                                       ttCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGaa                         >  1:1513782/1‑128 (MQ=255)
                                                                                                       ttCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACAACCATCTAATTGAATAATTGGACTAATTTCTTCTGGACTAACTGGaa                         >  2:1263763/1‑128 (MQ=255)
                                                                                                        tCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAAt                        >  1:548355/1‑128 (MQ=255)
                                                                                                         cTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAAtt                       <  1:1585979/128‑1 (MQ=255)
                                                                                                          tttAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAAtt                                                             <  2:1603365/89‑1 (MQ=255)
                                                                                                           ttAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTAc                     >  1:674382/1‑128 (MQ=255)
                                                                                                            tAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAAATGAATAATTGGACGAATGTCTGGTGGAATAACTCTAATTAct                    >  1:374043/1‑128 (MQ=255)
                                                                                                              aCTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTTGATTAATAACTGGAATTActct                  >  2:266179/1‑128 (MQ=255)
                                                                                                                 ttttttTATCTTTTATTTCTAATAATGAGTCTTCTATATAAATGATTCTTTTCAGATGTAGTCAATCTCCCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCAt               <  1:1812594/128‑1 (MQ=255)
                                                                                                                   ttttAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGa                                   >  2:594360/1‑106 (MQ=255)
                                                                                                                        aTCTTTTATTTCTAATAATGTTTCTTCTATATAACTCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcatt        <  1:801976/128‑1 (MQ=255)
                                                                                                                        aTCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcatt        >  2:150517/1‑128 (MQ=255)
                                                                                                                        aTCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcatt        <  2:1629341/128‑1 (MQ=255)
                                                                                                                        aTCTTTCATTTCAAATAATGATTCTTCTATATATATCAATAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGTATAATTGGTCGAATGTCTGGTGGTATAACTGTAATTACTCTCATAACcatt        <  2:830456/128‑1 (MQ=255)
                                                                                                                           tttCATTTCTAATAATGATACTACTATATAAAACATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTCGTGGAATAACTGGAATTACTCTCATAACcattcat     >  1:1605684/1‑128 (MQ=255)
                                                                                                                              cATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGTACTAATGTCTGTTTGAATAACTTGAATTACTCTCATAACcattcataaa  >  1:407197/1‑125 (MQ=255)
                                                                                                                              |                                                                                                                               
TACCTTGAACTGGTTTTGGTTTTCTTTCATTATCAAATAGAGCATCAACAGCTTCTTGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCA  >  CP016816/478556‑478809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑