Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 20,023 2 bp→TA intergenic (‑744/‑392) JCVSYN2_00065 ← / → JCVSYN2_00070 hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP01681620,0230AT99.7% 6282.5 / NA 2167intergenic (‑744/‑393)JCVSYN2_00065/JCVSYN2_00070hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand):  ref base A (1/5);  new base T (1166/992);  total (1170/997)
Fisher's exact test for biased strand distribution p-value = 1.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP01681620,0240TA99.7% 6450.5 / NA 2167intergenic (‑745/‑392)JCVSYN2_00065/JCVSYN2_00070hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand):  ref base T (4/3);  new base A (1166/994);  total (1170/997)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2172 total aligned reads displayed.

GCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATAATTTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACAAAAAAA  >  CP016816/19901‑20144
                                                                                                                          ||                                                                                                                        
gCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTta                                                                                                                      >  2:1419170/1‑128 (MQ=255)
       gcgcaAGAGAACCGGTCCTATATGTCGGCGATCCCCCTAGAGGCATTAACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAA                                                                                                               <  1:2794481‑M1/126‑1 (MQ=255)
         gCATGATTATAGGTAGTATTTGTTGGCGATCTGACTAGAGTCTTTTACATCTTCGGAATACTAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAA                                                                                                             <  2:4000481‑M1/128‑1 (MQ=255)
         gCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAA                                                                                                             >  2:554870‑M1/1‑128 (MQ=255)
         gCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAA                                                                                                             <  2:4226776‑M1/128‑1 (MQ=255)
         gCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAA                                                                                                             >  1:2328867‑M1/1‑128 (MQ=255)
             gATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTa                                                                                                         <  1:1000999‑M1/128‑2 (MQ=255)
                aaaaGGTAGTATTTGTTGTAGATACAACTAGAGTCTTTTACATATTAGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattg                                                                                                      <  2:3279879‑M1/128‑5 (MQ=255)
                  aaGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgta                                                                                                    >  2:3110254‑M1/1‑122 (MQ=255)
                   aGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtac                                                                                                   <  1:2582869‑M1/128‑8 (MQ=255)
                    ggTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtaca                                                                                                  <  2:1358042‑M1/128‑9 (MQ=255)
                     gtagtaTTAGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacag                                                                                                 <  1:1474352‑M1/128‑10 (MQ=255)
                      tagtaTTTGTTGGCGATCACCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagt                                                                                                >  1:1323474‑M1/1‑118 (MQ=255)
                       agtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagtt                                                                                               >  2:869057‑M1/1‑117 (MQ=255)
                        gtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAACCAAAAACTATATATTCTTTAATTTCTTTTTTTACTTTATATTTTTAATTTATATATTTATATTAAAAAATTattgtacagatg                                                                                              >  1:3524043‑M1/1‑116 (MQ=255)
                        gtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATTTTAAAAAATTattgtacagttg                                                                                              >  1:2883381‑M1/1‑116 (MQ=255)
                        gtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttg                                                                                              >  1:4308403‑M1/1‑116 (MQ=255)
                        gtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttg                                                                                              >  1:3251180‑M1/1‑116 (MQ=255)
                        gtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttg                                                                                              >  2:3941038‑M1/1‑116 (MQ=255)
                        gtaTTTGTTGGCGATCACCCTAGAGTCTATTACATCTTCGGTAAACAAAAACTATTTTTTCTTAAATTTCTTTTTTTACTTTCTATTTTAATTTTATATATTTATATTAATAAAttatttaacagatg                                                                                              >  2:2146521/1‑116 (MQ=255)
                         taTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgc                                                                                             >  1:2275845‑M1/1‑115 (MQ=255)
                         taTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTATTAATTTATATATTTATATTAAAAAATTattgtacagtgcc                                                                                             >  2:788430‑M1/1‑115 (MQ=255)
                          aTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgct                                                                                            >  2:714564‑M1/1‑114 (MQ=255)
                           tttGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATATTTAATTTATATATTTATATTAAAAAATTattgtacagttgcta                                                                                           >  2:2717715‑M1/1‑113 (MQ=255)
                             tgttgGCGATCCCACTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctact                                                                                         <  2:486917‑M1/128‑18 (MQ=255)
                               ttgGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactat                                                                                       >  2:1078046‑M1/1‑109 (MQ=255)
                                 ggCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataa                                                                                     <  1:2141273‑M1/128‑22 (MQ=255)
                                   cGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTATAAAATTattgtacagttgctactataagg                                                                                   >  2:1239842‑M1/1‑105 (MQ=255)
                                   cGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagg                                                                                   >  1:4202638‑M1/1‑105 (MQ=255)
                                    gATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataaggg                                                                                  >  2:3883315‑M1/1‑104 (MQ=255)
                                    gATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATACTTATATTAAAAAATTattgtacagttgctactataaggg                                                                                  >  2:117799‑M1/1‑104 (MQ=255)
                                     aTCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataatgga                                                                                 >  2:2310766‑M1/1‑103 (MQ=255)
                                        ccccTAGAGTCTTTTACATCTTCGGAAAACAATAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaag                                                                              >  2:4151935‑M1/1‑100 (MQ=255)
                                        ccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagtgaaag                                                                              >  2:2502212‑M1/1‑100 (MQ=255)
                                         cccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaaga                                                                             >  2:623348‑M1/1‑99 (MQ=255)
                                         cccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaaga                                                                             >  1:4456901‑M1/1‑99 (MQ=255)
                                           cTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaa                                                                           >  2:2402810‑M1/1‑97 (MQ=255)
                                           cTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAATTattgtacagttgctactataagggaaagaaaa                                                                          >  2:2066238‑M1/1‑96 (MQ=255)
                                            tAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAATAATTattgtacagttgctactataagggtaagataa                                                                          >  1:1893316‑M1/1‑96 (MQ=255)
                                            tAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaa                                                                          >  2:9131‑M1/1‑96 (MQ=255)
                                               agTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaa                                                                       >  2:4331408‑M1/1‑93 (MQ=255)
                                                 tCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaaga                                                                     >  2:4540216‑M1/1‑91 (MQ=255)
                                                 tCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaaga                                                                     <  1:1267701‑M1/128‑38 (MQ=255)
                                                  cTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaa                                                                       >  1:4128273‑M1/1‑90 (MQ=255)
                                                    tttACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaag                                                                  <  2:3191687‑M1/128‑41 (MQ=255)
                                                      tACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagat                                                                <  1:2202860‑M1/128‑43 (MQ=255)
                                                       aCATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagtttctactataagggaaagaaaaaaagaaagata                                                               >  2:3370310‑M1/1‑85 (MQ=255)
                                                       aCATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagata                                                               >  1:3920882‑M1/1‑85 (MQ=255)
                                                        cATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatat                                                              >  1:1049402‑M1/1‑84 (MQ=255)
                                                        cATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatat                                                              >  1:3979359‑M1/1‑84 (MQ=255)
                                                         aTCTTCTGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacaggtgctactataagggaaagaaaaaaagaaagatata                                                             <  1:358488‑M1/128‑46 (MQ=255)
                                                          tCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataa                                                            >  2:4437022‑M1/1‑82 (MQ=255)
                                                           cTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaa                                                           <  1:3008138‑M1/128‑48 (MQ=255)
                                                           attatgaaaacATAAAATATTATTTATTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataattgaaagaaaaaaagaaagatataaa                                                           <  1:1408017‑M1/123‑48 (MQ=255)
                                                            ttCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaat                                                          <  2:3443336‑M1/128‑49 (MQ=255)
                                                             tCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaataaagaaagatataaatt                                                         >  1:512710‑M1/1‑79 (MQ=255)
                                                               ggAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgt                                                       >  1:1587002‑M1/1‑77 (MQ=255)
                                                               ggAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgt                                                       >  2:1834387‑M1/1‑77 (MQ=255)
                                                                gAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgta                                                      <  1:4377853‑M1/128‑53 (MQ=255)
                                                                 aaaaCAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtat                                                     >  1:2739762‑M1/1‑75 (MQ=255)
                                                                   aaCAAAAACTAATTTTTCTTTAATTTCTATTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataa                                                   <  2:1741443‑M1/128‑56 (MQ=255)
                                                                       aaaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagta                                               >  1:3370602‑M1/1‑69 (MQ=255)
                                                                         aaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagg                                             <  2:3654668‑M1/128‑62 (MQ=255)
                                                                         aaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagg                                             <  1:3749668‑M1/128‑62 (MQ=255)
                                                                          aCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtaggg                                            >  1:2661515‑M1/1‑66 (MQ=255)
                                                                          aCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaaatgtataaagtatgg                                            >  2:4090040‑M1/1‑66 (MQ=255)
                                                                            tATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATATATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagggtt                                          >  1:1111242‑M1/1‑64 (MQ=255)
                                                                             aTTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTGTATATTTATATTAAAAAATTattgtacagtttctactataagttaaagaaaaaaagaaagatataaattgtataaattaggttta                                         >  2:1897217‑M1/1‑63 (MQ=255)
                                                                              ttttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaatatataaattgtataaagtatggttag                                        >  2:2226935‑M1/1‑62 (MQ=255)
                                                                              ttttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagggttag                                        <  2:2810367‑M1/128‑67 (MQ=255)
                                                                              ttttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagggttag                                        >  1:3146459‑M1/1‑62 (MQ=255)
                                                                               tttttATTTAATTTCATTATTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagggttaga                                       <  2:1986262‑M1/128‑68 (MQ=255)
                                                                                ttttCTTTAAATTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagggttagaa                                      <  1:241619‑M1/128‑69 (MQ=255)
                                                                                 tttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagggttagaag                                     <  2:35244‑M1/128‑70 (MQ=255)
                                                                                    cTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagggtttgaagcaa                                  >  1:2067117‑M1/1‑56 (MQ=255)
                                                                                    cTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagggttagaagcaa                                  >  2:1634644‑M1/1‑56 (MQ=255)
                                                                                    cTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagggttagaagcaa                                  >  1:1802422‑M1/1‑56 (MQ=255)
                                                                                      ttAATTTCTTTTTTTACTTTGTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagatgctactataagggaaagaaaaaaagaaagatataaattgtataaagtaggggtagcagcaatt                                <  2:2909907‑M1/128‑75 (MQ=255)
                                                                                       tAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagggttagaagcaatta                               >  2:3039504‑M1/1‑53 (MQ=255)
                                                                                        aaTTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagggttagaagcaattaa                              <  1:1707114‑M1/128‑77 (MQ=255)
                                                                                           ttCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagggttagaagcaattaataa                           >  1:588061‑M1/1‑49 (MQ=255)
                                                                                            tCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagggttagaagcaattaataat                          >  1:738360‑M1/1‑48 (MQ=255)
                                                                                                  tttAGTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagggttagaagcaattaataattattat                    <  1:1999925‑M1/128‑87 (MQ=255)
                                                                                                   ttACTTTATATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagggttagaagcaattaataattattatt                   <  1:1193787‑M1/128‑88 (MQ=255)
                                                                                                    tACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagggttagaagcaattaataattattatta                  <  1:968218‑M1/128‑89 (MQ=255)
                                                                                                    tACTTTATTTTTTTAATTTATATATTTATATTAAAAATTTattgtacagttgctactataagtgaaagaaaaaaagaaagatataaattgtataaagtagggttagaagcaattaataattattatta                  <  1:3835841‑M1/128‑89 (MQ=255)
                                                                                                     aCTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagggttagaagcaattaataattattattaa                 >  2:2600668‑M1/1‑39 (MQ=255)
                                                                                                      cTTTCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagggttagaagcaattaataattattattaat                <  1:649188‑M1/128‑91 (MQ=255)
                                                                                                      cTTTCTATTTTTAATTTATATAATTATATAAAAAAAATattgtacagatgctactataagggaaataaaaaaagaaagatatatattgtataatgtagggttagaagcaattaataattattattaat                <  1:3081636‑M1/128‑91 (MQ=255)
                                                                                                        ttCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagggttagaagcaattaataattattattaatgt              <  2:3727297‑M1/128‑93 (MQ=255)
                                                                                                         tCTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagggttagaagcaattaataattattattaatgtt             >  1:2496791‑M1/1‑35 (MQ=255)
                                                                                                          cTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagggttagaagcaattaataattattattaatgtta            >  2:3808271‑M1/1‑34 (MQ=255)
                                                                                                          cTATTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagggttagaagcaattaataattattattaatgtta            >  1:455479‑M1/1‑34 (MQ=255)
                                                                                                            aTTTTTAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagggttagaagcaattaataattattattaatgttatt          >  1:3674585‑M1/1‑32 (MQ=255)
                                                                                                             tttttAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagggttagaagcaattaataattattattaatgttattt         <  1:807978‑M1/128‑98 (MQ=255)
                                                                                                             tttttAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagggttagaagcaattaataattattattaatgttattt         <  2:153401‑M1/128‑98 (MQ=255)
                                                                                                               tttAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagggttagaagcaattaataattattattaatgttattttt       <  1:4066142‑M1/128‑100 (MQ=255)
                                                                                                               ttaaatttATATAATTATATTAAAAAATTattgtacagttgatactataagtgaaagaaaaaaagaaagatataaattttataaagtagggttagaagcaattaataattattattaatgttattttt       <  1:895971‑M1/125‑100 (MQ=255)
                                                                                                                ttAATTTATATATTTATATTAAAAAATTattgtacagttgctactataagggaaagaaaaaaagaaagatataaattgtataaagtagggttagaagcaattaataattattattaatgttatttttc      <  2:2970055‑M1/128‑101 (MQ=255)
                                                                                                                    tttATATATTTATATTAAAAAATTattgtacagttgctactataaggtaaagaaaaaaagaaagatataaattgtagaaagtagggttagaagaaattaataatttttattaatgttatttttctcta  >  1:3446262‑M1/1‑24 (MQ=255)
                                                                                                                          ||                                                                                                                        
GCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATAATTTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACAAAAAAA  >  CP016816/19901‑20144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: