BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	192,836	C→A	H133N (CAT→AAT)	JCVSYN2_00800 →	transketolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	192,836	0	C	A	98.6%	4513.9 / NA	1608	H133N (CAT→AAT)	JCVSYN2_00800	transketolase
Reads supporting (aligned to +/- strand): ref base C (4/3); major base A (809/766); minor base T (17/6); total (833/775)
Fisher's exact test for biased strand distribution p-value = 3.57e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1609 total aligned reads displayed.

TTAGACAATGAGATAGTAAAACTCCAGGACATCCTGAAAAAACTTTAACGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTCATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACAAGAAGGAATTAGTCAAGAAGCTATTTCTTTTGCTGGTAAACA  >  CP016816/192711‑192960

 tAGACACTGAGATAGTAAAACGCCAGGACATCCTGAAAAACCTTTAACGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATc                                                                                                                           <  1:4539583/128‑1 (MQ=255)

  cgACAATGAGATAGTAAAACTCCAGGACATCCTGAAAAAACTTTAACGGGTGGAGGTGAAGTGACACCAGGTCCACTTGGGCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCt                                                                                                                          <  1:118420/127‑1 (MQ=255)

    aCAATGAGATAGTAAAACTCCAGGACATCCTGAAAAAACTTTAACGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAg                                                                                                                        >  1:2507484/1‑128 (MQ=255)

     cAATGAGATAGTAAAACTCCAGGACATCCTGAAAAAACTTTAACGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGc                                                                                                                       <  2:4551454/128‑1 (MQ=255)

       aTGAGATAGTAAAACTCCAGGACATCCTGAACAAACTTTAACGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCaa                                                                                                                     <  2:3393138/128‑1 (MQ=255)

       aTGAGATAGTAAAACTCCAGGACATCCTGAAAAAACTTTAACGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCaa                                                                                                                     <  2:3237830/128‑1 (MQ=255)

       aTGAGATAGTAAAACTCCAGGACATCCTGAAAAAACTTTAACGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGGTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCaa                                                                                                                     >  1:1461378/1‑128 (MQ=255)

        tGAGATAGTAAAACTCCAGGACATCCTGAAAAAACTTTAACGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAAGCTAGCAAg                                                                                                                    >  1:3631331/1‑128 (MQ=255)

        tGAGATAGTAAAACTCCAGGACATCCTGAAAAAACTTTAACGTGAGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAg                                                                                                                    >  2:3747852/1‑128 (MQ=255)

          agaTAGTAAAACTCCAGGACATCCTGAAAAAACTTTAACGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAgtg                                                                                                                  <  2:2562395/128‑1 (MQ=255)

          agaTAGTAAAACTCCAGGACATCCTGAAAAAACTTTAACGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAgtg                                                                                                                  <  1:4470827/128‑1 (MQ=255)

           gagaGTACAACTCCAGGACATCCTGAAAAACCTTTACCGTGTGGAGTTGAAGTGACAACAGGGCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAgtgt                                                                                                                 <  2:764164/125‑1 (MQ=255)

             tAGTAAAACTCCAGGACATCCTGAAAAAACTTTAACGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGTATGGCTGTTGCTGAAGCTACTCTAGCTAGTGgtt                                                                                                               >  2:1083204/1‑128 (MQ=255)

              aGTAACACTCCAGGACATCCTGAAAAAACTTTAACGTGGGGAGTTGAAGTGACAACAGGTCCACTTGGGCAAGGGGTTGGAATGGGAGTTGGAATGGCGGTTGCTGAAGCTAATCTAGCAAGTGTTta                                                                                                              <  2:113705/128‑1 (MQ=255)

                tAAAACTCCAGGACATCCTGAAAAAACTTTAACGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGTATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGGAAgaggatctg                                                                                                            >  1:4139851/1‑120 (MQ=255)

                  acaCTCCAGGACATCCTGAAAAAACTTTTACGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTataat                                                                                                          <  2:1492097/126‑1 (MQ=255)

                  aaaCTCCAGGACATCCTGAAAAAACTTTAACGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCCAGCAAGTGTTTataat                                                                                                          <  2:2368886/128‑1 (MQ=255)

                    aCTCCAGTACATCCTGAAAAAACTTTAACGTGTGGAGTTGAAGTGACAACAGGTCCCCTTGGGAATGGGGTGGGAATGGGAGTGGGGATGGCTGTGGCGGACGCTATGGTAGCATGGGTTTataatta                                                                                                        >  2:2098762/1‑126 (MQ=255)

                    aCTCCAGGACATCCTGAAAAAACTTTAACGTGTGGAGTTGAAGTGACCACAGGTCCACTTGCTCAAGGAGTTGGAATGGGAGTGGGAAGGGCTGTTGCTGAAGCTAATCTAGCAAGTGTGtagactca                                                                                                        >  1:2396889/1‑122 (MQ=255)

                    aCTCCAGGACATCCTGAAAAAACTTTAACGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTAtaataa                                                                                                        >  1:2820995/1‑128 (MQ=255)

                       ccAGTACTTACTGATATATCTTTAACGTTTGGAGTTGAAGTGACAACAGGTCAACTTGGTCAAGGTGTTGGAATGTGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACa                                                                                                     <  2:4466698/128‑1 (MQ=255)

                       ccAGGACATCCTGAAAAAACTTTAACGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACa                                                                                                     <  1:3281960/128‑1 (MQ=255)

                       ccAGGACATCCTGAAAAAACTTTAACGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACa                                                                                                     <  2:2672794/128‑1 (MQ=255)

                          ggACATCCTGAAAAAACTTTAACGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCa                                                                                                                      >  1:3184195/1‑108 (MQ=255)

                          ggACATCCTGAAAAAACTTTAACGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATAGAGCCAGTGTTTATAATAAACATGa                                                                                                  >  2:911222/1‑128 (MQ=255)

                            aCATCCTGAAATAACTTTAACGTGTGGAGTTGAAGTGACAACAGCTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTATCAAGTGTTTATAATAAACATGAtt                                                                                                <  2:661721/128‑1 (MQ=255)

                               tCCTGAAAAAACTTTAACGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTa                                                                                             >  1:3844589/1‑128 (MQ=255)

                                ccTGAAAAAACTTTAACGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTaa                                                                                            >  2:2820355/1‑128 (MQ=255)

                                   gAAAAAACTTTAACGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGGTGGAATGGGAGTTGGACTGGCTGTTGCTGCAGCTAAGCTAGCAAGTGTTTATAATATACTGGATGTTAAgtt                                                                                         >  1:4260286/1‑128 (MQ=255)

                                    aaaaaaCTTTAACGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGTGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATATACATGCTTTTAAtttt                                                                                        >  1:1708793/1‑127 (MQ=255)

                                         aCTTTAACGTGTGGAGTTGAAGTGACAACAGGTCCACGTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATATTAAAGATGATGTTATTTTGATtca                                                                                   >  2:3062562/1‑128 (MQ=255)

                                         aCTGTAACGTGTGGAGTGGAAGTGACAACAGGTACACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATtca                                                                                   <  1:852952/128‑1 (MQ=255)

                                          cTTTAACGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATtcat                                                                                  <  2:4062243/128‑1 (MQ=255)

                                             tAACGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTcatcat                                                                               <  1:4215049/128‑1 (MQ=255)

                                              aatgtgtgtAGTTGAAGTGACAACTGGTCCAGTTTGTCAAGGTGTTGGAATTTTATTTTTATTGTATTTTGATGAAGCTAATCTATCAAGTTTTTATAATAAAAATGATTTTAATTTGATTCATCAtt                                                                              <  2:1002304/125‑1 (MQ=255)

                                               aCGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATTGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATta                                                                             <  2:555110/128‑1 (MQ=255)

                                               aCGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGCAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATta                                                                             >  2:3469100/1‑128 (MQ=255)

                                               aCGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTataa                                                                                                           >  1:106562/1‑98 (MQ=255)

                                                cGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATtat                                                                            >  1:4314372/1‑128 (MQ=255)

                                                 ctgtgGAGTGGCAGGGACAACAGGGCCACTTTGTCAAGGTGTTGGAATGGGAGTTGTAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATtata                                                                           <  1:3482183/127‑1 (MQ=255)

                                                    tgGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGATTGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAAGTTGATTCATCAGTATACtt                                                                        >  2:196223/1‑128 (MQ=255)

                                                        gTTGAAGTTAAACTAGGTCCAGTTGGTCAAGGTGGTGTAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATATAGAAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGt                                                                    <  2:2295895/128‑1 (MQ=255)

                                                         ttGAAGTGACGACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGtt                                                                   >  2:2730221/1‑128 (MQ=255)

                                                         ttGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGATTGGGAGTTGGAATGGCTGTTGCTGACGCTAATCTAGCATGTGTTGATAATAAACATGATTTTAAGTTGAGTCATCAGTATACTTATGtt                                                                   >  2:3141339/1‑128 (MQ=255)

                                                         ttGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGtt                                                                   <  1:1631220/128‑1 (MQ=255)

                                                          tGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGttt                                                                  >  1:2678638/1‑128 (MQ=255)

                                                               tGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGTCTGTTGCTGAAGCTTATCTAGGAAGTCTTTATAATAAACATGAATATAATATGATTCATCATTAGACTTATCTTGTAtgt                                                             >  1:4375375/1‑128 (MQ=255)

                                                               ggACCACAGGTCCACTTGGGCAAGGTGGTGGAATGGGAGTTGGAATGGCTGTGGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTAtgt                                                             <  2:4148422/127‑1 (MQ=255)

                                                                gACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTAtgtg                                                            <  1:2035069/128‑1 (MQ=255)

                                                                   aacaGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATgtggtg                                                         <  2:3793455/128‑1 (MQ=255)

                                                                      aGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGtggtgatg                                                      <  2:24711/128‑1 (MQ=255)

                                                                       ggTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGtggtgatgg                                                     >  2:1235383/1‑128 (MQ=255)

                                                                       ggTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGtggtgatgg                                                     <  1:3925754/128‑1 (MQ=255)

                                                                       ggTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGtggtgatgg                                                     >  2:1682634/1‑128 (MQ=255)

                                                                         gtcACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGAGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATATTTTATGttgtgatggat                                                   >  1:1281693/3‑126 (MQ=255)

                                                                          ccACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGtggtgatggtga                                                  >  1:2226194/1‑128 (MQ=255)

                                                                          ccACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGtggtgatggtga                                                  <  2:504447/128‑1 (MQ=255)

                                                                          ccACTTGGTCAAGGTGTTGGAATGGGAGTGGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGtggtgatggtga                                                  <  2:3003766/128‑1 (MQ=255)

                                                                            acttcggctaGGCGTTGTAATGGGAGTTGGAATTGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATACTAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGAtt                                                <  1:1568971/119‑1 (MQ=255)

                                                                            aCTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGAtt                                                >  2:2621035/1‑128 (MQ=255)

                                                                               tGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATGATTATACTTATGTGTTATGTGGTGATGGTGATTTAc                                             >  1:3400084/1‑128 (MQ=255)

                                                                               tGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTAc                                             <  1:2751457/128‑1 (MQ=255)

                                                                                ggTCATGGTTGTGGAAGGGGAGGTGGTTTGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGTTTCATCATAATACTTATGTTTTATGTGGTGATGGTGATTTACa                                            <  1:1775553/128‑1 (MQ=255)

                                                                                ggTCAAGGTGTTTGTATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATATACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACa                                            <  2:4406684/128‑1 (MQ=255)

                                                                                 gTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACaa                                           >  2:1403733/1‑128 (MQ=255)

                                                                                  tCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACaag                                          <  1:296747/128‑1 (MQ=255)

                                                                                      ggTGTTGGAATGGGAGTTGGAATGTCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACAAGAAgg                                      <  2:1862560/128‑1 (MQ=255)

                                                                                      ggTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACAAGAAgg                                      >  2:1310258/1‑128 (MQ=255)

                                                                                      ggTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACAAGAAgg                                      >  1:633585/1‑128 (MQ=255)

                                                                                          ttGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGAGTTTAATTAGATTCATCATTATACTTATGTTTTATGTGGTGATGTTGATTTACATCAAGGAAtt                                  >  2:386033/1‑128 (MQ=255)

                                                                                           tGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACAAGAAGGAATTa                                 <  1:3709716/128‑1 (MQ=255)

                                                                                              actgtgAGTTGTAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAACCATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACAAGAAGGAATTAGTc                              <  2:2014942/123‑1 (MQ=255)

                                                                                              aaTGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACAAGAAGGAATTAGTc                              >  2:4323935/1‑128 (MQ=255)

                                                                                                tGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACAAGAAGGAATTAGTCaa                            >  1:3056840/1‑128 (MQ=255)

                                                                                                tGGGAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACAAGAAGGAAGTAGTCaa                            >  2:4231156/1‑128 (MQ=255)

                                                                                                ggggAGTTGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACAAGAAGGAATTAGTCaa                            <  1:2588070/127‑1 (MQ=255)

                                                                                                 gggAGCTTGTATGGCTGTTGAGGAAGCTAATCTAGCAAGCGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACAAGAAGGAATTAGTCaag                           <  1:3562339/128‑1 (MQ=255)

                                                                                                  tgAGTTGTAATGGCTGTTGCTGAAGATAATCTAGCAAGTGTTTATAATAAACATTATTTTAATTTGATTCATCATTATACTTATGATTTATGTTTTTATTGTTATTTACAATAATTAATTAGTCaata                          >  1:1954687/2‑126 (MQ=255)

                                                                                                    aGTTGGAATGGCTGTTGCTGAATCTAATCTAGCAAGTGGTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACAAGAAGGAATTAGTCaagaag                        <  1:539187/128‑1 (MQ=255)

                                                                                                    aGTTGGAATGGCTGTTGCTGAAGATAATCTATCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACAAGAAGGAATTAGTCaagaag                        <  1:1128038/128‑1 (MQ=255)

                                                                                                    aGTTGGAATGGCTGTTGCTGAAGATAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACAAGAAGGAATTAGTCaagaag                        <  2:1605541/128‑1 (MQ=255)

                                                                                                     gTTGGAATGGCTGTTGCTGAAGCTAATCTATCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATCTGGTGATGGTGATTTACAAGAAGGAATAACACAAGAAGc                       >  1:790279/1‑128 (MQ=255)

                                                                                                      ttGGAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGTTTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGGGATGGTGATTTACAAGAAGGAATTAGTCAAGAAGCt                      <  2:3646682/128‑1 (MQ=255)

                                                                                                        ggAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACAAGAAGGAATTAGTCAAGAAGCTAt                    >  1:7060/1‑128 (MQ=255)

                                                                                                        ggAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACAAGAAGGAATTAGTCAAGAAGCTAt                    >  2:81640/1‑128 (MQ=255)

                                                                                                        ggAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACAAGAAGGAATTAGTCAAGAAGCTAt                    >  1:2128568/1‑128 (MQ=255)

                                                                                                        ggAATGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTATATGTGGTGATGGTGATTTACAAGAAGGAATTAGTCAAGAAGCTAt                    >  1:4016979/1‑128 (MQ=255)

                                                                                                          aaTGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACAAGAAGGAAGTAGTCAAGAAGCTAttt                  >  1:2323582/1‑128 (MQ=255)

                                                                                                           aTGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACAAGAAGGAATTAGTCAAGAAGCTATTTc                 >  2:3564512/1‑128 (MQ=255)

                                                                                                            tGGCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACAAGAAGGAATTAGTCAAGAAGCTATTTCt                >  2:1757619/1‑128 (MQ=255)

                                                                                                             ggCTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACAAGAAGGAATTAGTCAAGAAGCTATTTCtt               >  2:2168480/1‑128 (MQ=255)

                                                                                                               cTGTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACAAGAAGGAATTAGTCAAGAAGCTATTTCtttt             >  1:1380737/1‑128 (MQ=255)

                                                                                                               cTGTTGCGGAAGCTAATCTAGCAAGTGTTTATAATAAACTTGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACAAGAAGGAATTAGTCAAGAAGCTATTTCtttt             <  2:2919703/128‑1 (MQ=255)

                                                                                                                 gTTGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGACTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACAAGAAGGAATTAGTCAAGAAGCTATTTCTTTTGc           >  2:394755/1‑128 (MQ=255)

                                                                                                                   tGCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACAAGAAGGAAGTAGTCAAGAAGCTATTACTTTTGCtc         >  1:2896122/1‑127 (MQ=255)

                                                                                                                    tcTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATAATTATACTTATGTTTAATGTGGTGATGGTGATTTACAAGAAGGAATTAGTCAAGAAGCTATTTCTTTTGCTgg        <  1:3776773/127‑1 (MQ=255)

                                                                                                                    gCTGAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATtat                                                                            <  1:924604/60‑1 (MQ=255)

                                                                                                                       gAAGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACAAGAAGGAATTAGTCAAGAAGCTATTTCTTTTGCTGGTaa     >  1:188483/1‑128 (MQ=255)

                                                                                                                         aGCTAATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACAAGAAGGAATTAGTCAAGAAGCTATTTCTTTTGCTGGTAAAc   <  1:4090674/128‑1 (MQ=255)

                                                                                                                         aGCTAATCTAGAAAGTGTTTATAATAATCTTGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGAGGGTGATTTACAAGAAGGAATTAGTCAAGAAGCTATTTCTTTTGCTGGTAAAc   <  1:1001109/128‑1 (MQ=255)

TTAGACAATGAGATAGTAAAACTCCAGGACATCCTGAAAAAACTTTAACGTGTGGAGTTGAAGTGACAACAGGTCCACTTGGTCAAGGTGTTGGAATGGGAGTTGGAATGGCTGTTGCTGAAGCTCATCTAGCAAGTGTTTATAATAAACATGATTTTAATTTGATTCATCATTATACTTATGTTTTATGTGGTGATGGTGATTTACAAGAAGGAATTAGTCAAGAAGCTATTTCTTTTGCTGGTAAACA  >  CP016816/192711‑192960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`