BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	273,187	C→T	E211K (GAA→AAA)	JCVSYN2_01135 ←	dihydroxyacetone kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	273,187	0	C	T	99.4%	6008.2 / NA	1926	E211K (GAA→AAA)	JCVSYN2_01135	dihydroxyacetone kinase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (907/1005); minor base A (10/2); total (919/1007)
Fisher's exact test for biased strand distribution p-value = 1.76e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1926 total aligned reads displayed.

TTGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTCTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGAATCAACAACCCCAACTTCTTTTAATAACGGTAATAAG  >  CP016816/273062‑273311

ttGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTa                                                                                                                            >  2:3752935/1‑128 (MQ=255)

 tGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAATGATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTaa                                                                                                                           <  1:3068364/128‑1 (MQ=255)

  gATCTCTTATTTCTGAGTTTTGTAATTTATTAATTCAATCATCATTTAGCATAACAATAGTTTCAGTACATTATCCAATTTCTTCTTCTATTTGCATATCAACATTTCCACGGTTATTAACTTTTAAt                                                                                                                          <  2:3131426/128‑1 (MQ=255)

  gATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCATCATTTCCACCATTATAAACTATTAAt                                                                                                                          >  2:1220758/1‑128 (MQ=255)

  gATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAAt                                                                                                                          >  2:1005364/1‑128 (MQ=255)

  gATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAAt                                                                                                                          >  2:3452876/1‑128 (MQ=255)

    tcttTAATGAATGAGTTTTGTAATTTATTAATTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAAttt                                                                                                                        <  1:4011251/128‑1 (MQ=255)

    tctcTAATGACTGAGTTTTGTAATTTATTAATTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAAttt                                                                                                                        <  1:3335097/128‑1 (MQ=255)

     ctctAATGACTGAGTTTTGTAATTTATTAATTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTa                                                                                                                       <  2:1573055/128‑1 (MQ=255)

      tctAATGACTGAGTTTTGTAATTTATTAATTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTAt                                                                                                                      <  1:2898516/128‑1 (MQ=255)

         aaTGACTGAGTTTTGTAATTTATTAATTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTa                                                                                                                   <  2:1508727/128‑1 (MQ=255)

            gACTGAGTTTTGTAATTTATTAATTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGtt                                                                                                                <  2:2547727/128‑1 (MQ=255)

            gACTGAGTTTTGTAATTTATTAATTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGtt                                                                                                                <  2:2396631/128‑1 (MQ=255)

             catGAGTTTTGTAATTTATTACTTCAATCATCATTTAGCATAACAATAGCTGCAGTACAATATCCAAATTCTTCTTCTATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGttt                                                                                                               <  1:3254245/126‑1 (MQ=255)

             aCTGAGTTTTGTAATTTATTAATTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGttt                                                                                                               <  1:1618930/128‑1 (MQ=255)

              cTGAGTTTAGTAATTTATTAATTCAATCATCATCTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTg                                                                                                              <  2:1910004/128‑1 (MQ=255)

               tGAGTTTTGTAATTTATTAATTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGt                                                                                                             >  2:2985259/1‑128 (MQ=255)

                 aGTGTTGTAATTTATTAAATCAATCATCATTTAGCATAAAAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGGTTGTTa                                                                                                           <  1:2256484/128‑1 (MQ=255)

                  gTTTTGTAATTTATTAATTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTaa                                                                                                          <  1:141131/128‑1 (MQ=255)

                  gTTTTGTAATTTATTAATTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTAGTTaa                                                                                                          >  1:3748733/1‑128 (MQ=255)

                    tttGTAATTTATTAATTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACa                                                                                                        >  2:851776/1‑128 (MQ=255)

                        tAATTTATTAATTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAAttt                                                                                                    >  2:2185494/1‑128 (MQ=255)

                          aTTTATTAATTCTATAATAATTTAGGATTTCAATAGGTTGAGTACAATATCCAAATTCTTATTGAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGa                                                                                                  <  2:2122316/128‑1 (MQ=255)

                          aTTTATTAATTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGa                                                                                                  >  2:4061197/1‑128 (MQ=255)

                          aTTTATTAATTCAATCATCATGTAGCATAACAATAGCGTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGa                                                                                                  <  2:4159869/128‑1 (MQ=255)

                           tttATTAATTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGAt                                                                                                 <  2:2474250/128‑1 (MQ=255)

                            ttattaATTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATc                                                                                                >  2:1827583/1‑128 (MQ=255)

                            ttattaATTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATc                                                                                                >  2:3699570/1‑128 (MQ=255)

                              attaATTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCAt                                                                                              <  2:1749122/128‑1 (MQ=255)

                                 aaTTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTa                                                                                           >  1:2179945/1‑128 (MQ=255)

                                 aaTTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTa                                                                                           >  2:2883765/1‑128 (MQ=255)

                                 aaTTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTa                                                                                           >  2:4477385/1‑128 (MQ=255)

                                  aTTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTaa                                                                                          <  2:2040417/128‑1 (MQ=255)

                                  aTTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTaa                                                                                          <  2:922388/128‑1 (MQ=255)

                                   ttCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTaaa                                                                                         >  1:891008/1‑128 (MQ=255)

                                    tCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTaaaa                                                                                        <  1:519011/128‑1 (MQ=255)

                                      aaTCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACa                                                                                      >  2:1413596/1‑128 (MQ=255)

                                      aaTCATCATTTAGCATAACAATAGCATAAGTAAAATATCCAAATTCTTCTTCAATTAGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACa                                                                                      <  1:4449186/128‑1 (MQ=255)

                                       ctcctcatTTAGCATAACAATAGATTCAGTACAATATCAAAATTCTTCATCAAATTGCATATAAACATTTCCACCGTTATTAACTTTTAATTTATCTAGCTTGTTAACAATTTGATCATTTAAAACAt                                                                                     <  1:3000897/124‑1 (MQ=255)

                                       atcatcATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACAt                                                                                     <  1:3832177/128‑1 (MQ=255)

                                         catcatTTAGCATACCTCTAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGATAACAATTTGATCATTTAAAACATAg                                                                                   <  1:1328607/128‑1 (MQ=255)

                                               ttAGCATAACAATAGCTTCAGTACAATATCCATATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCa                                                                             <  2:360811/128‑1 (MQ=255)

                                               ttAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCa                                                                             <  1:2484733/128‑1 (MQ=255)

                                               ttAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGAATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCa                                                                             <  1:3522702/128‑1 (MQ=255)

                                               ttAGCATAACAATAGCTTCAGTACAATATACAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCa                                                                             <  2:1984699/128‑1 (MQ=255)

                                                tAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCAACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCaa                                                                            >  1:1768164/1‑128 (MQ=255)

                                                  gCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATa                                                                          <  2:652560/128‑1 (MQ=255)

                                                      aaCAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCAtt                                                                                             >  2:3539336/1‑105 (MQ=255)

                                                        cAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTAGTCAATACCTTCt                                                                    >  2:2796936/1‑128 (MQ=255)

                                                           tAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTaaa                                                                 >  1:1104184/1‑128 (MQ=255)

                                                            aGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTaaaa                                                                >  2:1286011/1‑128 (MQ=255)

                                                             gCTTCAGTACAATATCCAAATTCTTCTTCAATTTTCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTaaaaa                                                               <  1:731541/128‑1 (MQ=255)

                                                             gCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTaaaaa                                                               >  1:3592727/1‑128 (MQ=255)

                                                               ttCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACt                                                             <  1:2802024/128‑1 (MQ=255)

                                                                 cAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTa                                                           >  2:1341247/1‑128 (MQ=255)

                                                                  aGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTaa                                                          <  1:1695/128‑1 (MQ=255)

                                                                  aGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATATAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTaa                                                          <  1:2105605/128‑1 (MQ=255)

                                                                     aCAATATCCTAATTCTTATTCATTTTGCATATAAACATATTCACCTTTATTAACTTATAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTa                                                       <  2:1173762/128‑1 (MQ=255)

                                                                      cAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACATTATAAAAACTTAAATaa                                                      >  1:4097172/1‑128 (MQ=255)

                                                                        atatCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATc                                                    <  1:1559382/128‑1 (MQ=255)

                                                                            ccAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATa                                                >  1:2435403/1‑128 (MQ=255)

                                                                            ccAAATTCTTCTTCAATTTGCATATCAACATTTCCACAGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATa                                                <  1:3678113/128‑1 (MQ=255)

                                                                             cAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATaa                                               >  1:4188945/1‑128 (MQ=255)

                                                                             cAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATaa                                               >  2:539767/1‑128 (MQ=255)

                                                                              aaaTTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAac                                              >  2:4369645/1‑128 (MQ=255)

                                                                                aTTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTATCTACTCCATAacca                                            >  1:2570615/1‑128 (MQ=255)

                                                                                  tcttcttcAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATa                                                                          <  1:831646/96‑1 (MQ=255)

                                                                                  tcttcttcAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTGAACTAATCCATAaccacc                                          >  2:4276359/1‑128 (MQ=255)

                                                                                  tcttcttcAATTAGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAaccacc                                          <  1:4239907/128‑1 (MQ=255)

                                                                                     tcttcAATTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGa                                       >  2:3850185/1‑128 (MQ=255)

                                                                                        tcAATTTGCATATCAACATTTCCACAGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGAATc                                    <  2:1695910/128‑1 (MQ=255)

                                                                                           aTTTGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGAATcaac                                 >  1:4306318/1‑128 (MQ=255)

                                                                                            tttGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGACTcaaca                                <  1:2353224/128‑1 (MQ=255)

                                                                                            tttGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGAATcaaca                                >  2:2701668/1‑128 (MQ=255)

                                                                                            tttGCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAAATTGATCATTTAAAACATAGTAATCAATACCTACTAAAAACTTAACTAATCCATAACCACCAGAATcaaca                                >  2:734040/1‑128 (MQ=255)

                                                                                             ttGCATATCAAGATTTCCACCGTTAATAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGAATcaacaa                               <  1:3169480/128‑1 (MQ=255)

                                                                                               gCATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGAATCAACAAcc                             <  1:629933/128‑1 (MQ=255)

                                                                                                cATATCAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGAATCAACAAccc                            <  1:453295/128‑1 (MQ=255)

                                                                                                  tatCAACATTTACACCGTTATTAAATTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGAATCAACAACCCCa                          <  1:2749758/128‑1 (MQ=255)

                                                                                                     cAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGAATCAACACCCCCAAct                       >  2:1093999/1‑128 (MQ=255)

                                                                                                     cAACATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGAATCAACAACCCCAAct                       >  2:3217196/1‑128 (MQ=255)

                                                                                                       aCATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGAATCAACAACCCCAActtc                     <  1:4358487/128‑1 (MQ=255)

                                                                                                       aCATTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGAATCAACAACACCAActtc                     >  1:2009364/1‑128 (MQ=255)

                                                                                                         aTTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGAATCAACAACCCCAActtctt                   >  2:3055313/1‑128 (MQ=255)

                                                                                                         aTTTCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGAATCAACAACCCCAActtctt                   >  1:1258569/1‑128 (MQ=255)

                                                                                                          tttCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGAATCAACACCCCCAACTTCttt                  >  1:3438085/1‑128 (MQ=255)

                                                                                                          tttCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGAATCAACAACCCCAACTTCttt                  >  2:1650345/1‑128 (MQ=255)

                                                                                                           ttCCACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGAATCAACAACCCCAACTTCtttt                 <  2:326659/128‑1 (MQ=255)

                                                                                                              cACCGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGAATCAACAACCCCAACTTCTTTtaat              <  1:1827583/128‑1 (MQ=255)

                                                                                                               aCCGTTATTAACTTTTAATTTCTCTAGTTTCTTAACAAGTTGATCATTTAAAACATAGTATTCTATACCTTCTAAAAACTTACCTAATCCATAACCACCAGAATCAACAACCCCAACTTCTTTtaata             <  2:4556227/128‑1 (MQ=255)

                                                                                                                 cGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGAATCAACAACCCCAACTTCTTTTAATAAc           <  2:231658/128‑1 (MQ=255)

                                                                                                                 cGTTATTAACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGAATCAACAACCCCAACTTCTTTTAATAAc           >  1:2652907/1‑128 (MQ=255)

                                                                                                                  gTTATTATATTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATATTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGAATCAACAACCCCAACTTCTTTTAATAACg          <  2:3351078/128‑1 (MQ=255)

                                                                                                                  gTTATTAACTTTTAATTTATCAAGTTTGTTAACAATTTGATCATTTTAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGAATCAACAACCCCAACTTCTTTTAATAACg          <  1:1005364/128‑1 (MQ=255)

                                                                                                                    tattaACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGAATCAACAACCCCAACTTCTTTTAATAACGGt        >  2:3962307/1‑128 (MQ=255)

                                                                                                                    tattaACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGAATCAACAACCCCAACTTCTTTTAATAACGGt        >  1:3897358/1‑128 (MQ=255)

                                                                                                                     attaACTATTAATTTATCTAGTTTGATAACAATATGATCATTTAAAACATAGTATTCAAAACCTTCTAAAAACTAAACTAATACATAACCACCAGAATCAACACCCCCAACAACTTTAAATAACGGta       >  1:1484360/1‑128 (MQ=255)

                                                                                                                      ttaACTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGAATCAACAACCCCAACTTCTTTTAATAACGGtaa      >  1:1182663/1‑128 (MQ=255)

                                                                                                                        aaCTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGAATCAACAACCCCAACTTCTTTTAATAACGGtaata    >  1:1908871/1‑128 (MQ=255)

                                                                                                                          cTTTTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGAATCAACAACCCCAACTTCTTTTAATAACGGTAATAAg  <  2:2629306/128‑1 (MQ=255)

TTGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAATCATCATTTAGCATAACAATAGCTTCAGTACAATATCCAAATTCTTCTTCAATTTGCATATCAACATTTCCACCGTTATTAACTTCTAATTTATCTAGTTTGTTAACAATTTGATCATTTAAAACATAGTATTCAATACCTTCTAAAAACTTAACTAATCCATAACCACCAGAATCAACAACCCCAACTTCTTTTAATAACGGTAATAAG  >  CP016816/273062‑273311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`