BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	242,857	G→A	G829D (GGT→GAT)	JCVSYN2_01035 →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	242,857	0	G	A	99.9%	6196.5 / NA	1970	G829D (GGT→GAT)	JCVSYN2_01035	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base G (1/0); major base A (983/981); minor base T (0/3); total (986/984)
Fisher's exact test for biased strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1970 total aligned reads displayed.

AAGAACTTGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGGTTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACGATTAAT  >  CP016816/242732‑242981

   aaCTTGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAg                                                                                                                         >  1:4204963/1‑128 (MQ=255)

    aCTTGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGc                                                                                                                        >  1:3544446/1‑128 (MQ=255)

    aCTTGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAAATGTTGTTGGAATTTCACCTATGTTGATTTCAGCAGATTTAGc                                                                                                                        >  1:3112294/1‑128 (MQ=255)

        gATTTAAAAAATCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTATAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAAtt                                                                                                                    <  1:702844/128‑1 (MQ=255)

        gATTTAAAAAAGCTAAGGTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTCTTGGAATTTCAACTATGTTTATTTCAGCAGATTGAGCCAtt                                                                                                                    >  1:1846101/1‑128 (MQ=255)

         aTTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTc                                                                                                                   >  2:1840048/1‑128 (MQ=255)

          tttAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTcc                                                                                                                  >  1:2819131/1‑128 (MQ=255)

          tttAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTcc                                                                                                                  <  1:953239/128‑1 (MQ=255)

           ttAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCa                                                                                                                 <  1:571201/128‑1 (MQ=255)

            tAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCAt                                                                                                                >  2:3845968/1‑128 (MQ=255)

            tAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCAt                                                                                                                >  2:3769194/1‑128 (MQ=255)

            tAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCAt                                                                                                                <  2:2771166/128‑1 (MQ=255)

             aaaaaaGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGATTTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATc                                                                                                               >  1:2259583/1‑128 (MQ=255)

              aaaaaGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCa                                                                                                              >  1:3471164/1‑128 (MQ=255)

                caaGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAAt                                                                                                            <  2:1967389/127‑1 (MQ=255)

                  aGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTg                                                                                                          <  2:2930393/128‑1 (MQ=255)

                   gCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGc                                                                                                         >  2:4202921/1‑128 (MQ=255)

                    cTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCt                                                                                                        >  1:4000728/1‑128 (MQ=255)

                    cTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCt                                                                                                        <  1:1005064/128‑1 (MQ=255)

                      aaGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCttt                                                                                                      <  2:2843304/128‑1 (MQ=255)

                       aGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTATGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCtttt                                                                                                     <  1:4054073/128‑1 (MQ=255)

                         tttACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTAc                                                                                                   >  2:2137592/1‑128 (MQ=255)

                         tttACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTAc                                                                                                   >  2:4116206/1‑128 (MQ=255)

                          ttACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACt                                                                                                  >  2:2280810/1‑128 (MQ=255)

                           tactacTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTa                                                                                                 >  1:1771081/1‑128 (MQ=255)

                              tactaAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTa                                                                                              <  2:1284685/128‑1 (MQ=255)

                                  aaaTTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTaaaaa                                                                                          <  1:437413/128‑1 (MQ=255)

                                    aTTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAaca                                                                                        >  1:2184291/1‑128 (MQ=255)

                                       tAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACtt                                                                                     >  2:3254931/1‑128 (MQ=255)

                                       tAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACtt                                                                                     >  2:3691471/1‑128 (MQ=255)

                                        aGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACtta                                                                                    <  1:642301/128‑1 (MQ=255)

                                          tttatctttatcTTCTTATGTTAAAAAAAATATCTTTTTATTTGTAACTGTTGTTGGAATTTAAACTATTTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACttatt                                                                                  <  2:923577/128‑1 (MQ=255)

                                          tttAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACttatt                                                                                  <  2:834082/128‑1 (MQ=255)

                                          tttAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACttatt                                                                                  >  1:3374614/1‑128 (MQ=255)

                                              aCTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCATCTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATaa                                                                              >  2:2363542/1‑128 (MQ=255)

                                               cTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACtta                                                                                    >  2:4022033/1‑121 (MQ=255)

                                               cTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATaaa                                                                             <  2:353617/128‑1 (MQ=255)

                                               cTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATaaa                                                                             <  2:660738/128‑1 (MQ=255)

                                               cTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATaaa                                                                             >  1:3914036/1‑128 (MQ=255)

                                                  tAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGt                                                                          >  2:3362806/1‑128 (MQ=255)

                                                       tcttctTGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTaa                                                                     <  2:1209459/128‑1 (MQ=255)

                                                          tctGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTTCTATTAAAAACACTTATTATAAAAGTATTAAata                                                                  >  1:2864250/1‑128 (MQ=255)

                                                           ctGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAatat                                                                 <  2:4533112/128‑1 (MQ=255)

                                                             ggTAAAAATAATATCTTTTTATTAGTATCTGTTGTTGGAATTTCAACTATGTTTCTTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGc                                                               <  1:2418904/128‑1 (MQ=255)

                                                             ggTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGc                                                               <  2:3923494/128‑1 (MQ=255)

                                                              taaaaaaaaaTATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCa                                                              <  2:1019208/126‑1 (MQ=255)

                                                                aaaaaaaaTATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCaaa                                                            >  2:4281729/1‑128 (MQ=255)

                                                                  aaaaaaTATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATg                                                          >  2:4467555/1‑128 (MQ=255)

                                                                  aaaaaaTATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATg                                                          <  2:3458411/128‑1 (MQ=255)

                                                                   aaaaaTATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGa                                                         >  1:3614530/1‑128 (MQ=255)

                                                                   aaaaaTATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGa                                                         >  1:794529/1‑128 (MQ=255)

                                                                    aaaaTATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGaa                                                        >  1:4489446/1‑128 (MQ=255)

                                                                    aaaaTATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGaa                                                        >  2:3161918/1‑128 (MQ=255)

                                                                        tatCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAAtata                                                    <  1:3691471/128‑1 (MQ=255)

                                                                          tCTTTTTATTAGTAACTGTTGTTGGAATTTCTACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAat                                                  <  2:1106372/128‑1 (MQ=255)

                                                                          tCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAat                                                  >  1:1287017/1‑128 (MQ=255)

                                                                            tttttATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAatta                                                >  2:1666273/1‑128 (MQ=255)

                                                                            tttttATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAatta                                                <  2:723005/128‑1 (MQ=255)

                                                                            tttttATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCCTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAatta                                                >  2:4363974/1‑128 (MQ=255)

                                                                               tttttaGTAAATGTTGTTTGAATTTAAACTTTGTTTATTTCAGCAGATTTATCAATTACATCAATTGATTTTACTATTAAAAACTCTTATTATTAAAGTATTAAATATGCAAATGAATATAATTATTc                                             <  1:2949296/125‑1 (MQ=255)

                                                                                tattaGTTACTGTTGTTGGAATTTCAACTATGTTTATTTAAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCa                                            <  2:2665511/128‑1 (MQ=255)

                                                                                tattaGTTACTGTTGTTGGAAATTCTACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACAATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATTTAATTATTCa                                            <  1:1669654/128‑1 (MQ=255)

                                                                                tattaGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCa                                            >  1:2045247/1‑128 (MQ=255)

                                                                                 attaGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCaa                                           >  1:2129161/1‑128 (MQ=255)

                                                                                  ttaGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAATTATTAAATATGCAAATGAATATAATTATTaaaa                                          >  2:3004355/1‑128 (MQ=255)

                                                                                  ttaGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCaaa                                          <  1:122755/128‑1 (MQ=255)

                                                                                   taGTAACTGTTGTTTGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTACATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCaaaa                                         <  1:2658326/128‑1 (MQ=255)

                                                                                     gTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAgg                                       >  1:1093834/1‑128 (MQ=255)

                                                                                     gTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAgg                                       <  2:2449237/128‑1 (MQ=255)

                                                                                         cTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGtt                                   >  2:1363619/1‑128 (MQ=255)

                                                                                          tgttgtTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGttt                                  <  2:2206165/128‑1 (MQ=255)

                                                                                          tgttgtTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGttt                                  <  1:3274539/128‑1 (MQ=255)

                                                                                          tgttgtTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGttg                                  >  2:581465/1‑127 (MQ=255)

                                                                                           gttgttGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTc                                 <  1:4292273/128‑1 (MQ=255)

                                                                                              gttgGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCaaa                              <  1:3769194/128‑1 (MQ=255)

                                                                                                 ggAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATtc                           >  1:1935889/1‑128 (MQ=255)

                                                                                                  gAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATtct                          >  2:3610204/1‑128 (MQ=255)

                                                                                                   aaTTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATtctc                         >  2:3084777/1‑128 (MQ=255)

                                                                                                   aaTTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATtctc                         >  2:503882/1‑128 (MQ=255)

                                                                                                   aaTTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATtctc                         >  1:3043138/1‑128 (MQ=255)

                                                                                                   aaTTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTCTTTCAAATtctc                         >  1:1433224/1‑128 (MQ=255)

                                                                                                     tttCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCt                       <  1:873962/128‑1 (MQ=255)

                                                                                                        cAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTa                    >  1:2592894/1‑128 (MQ=255)

                                                                                                             aTGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACaaa               >  1:3831057/1‑128 (MQ=255)

                                                                                                              tGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACaaaa              <  2:1759422/128‑1 (MQ=255)

                                                                                                               gTTTATTTCAGCAGATTTATCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTAATAAATATGCAAATTAATATAATAATTCAAAATTTGATTCAAATTCTCCTTTAACAacac             >  1:3177770/1‑128 (MQ=255)

                                                                                                               gTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAAc             >  1:2313891/1‑128 (MQ=255)

                                                                                                               gTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAAc             >  1:2516210/1‑128 (MQ=255)

                                                                                                                tttATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACACAAcc            >  1:1363410/1‑128 (MQ=255)

                                                                                                                tttATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAAcc            >  1:4565778/1‑128 (MQ=255)

                                                                                                                tttATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAAcc            >  2:2566998/1‑128 (MQ=255)

                                                                                                                tttATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAAcc            >  2:1421837/1‑128 (MQ=255)

                                                                                                                  tATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCaa          <  2:2064809/128‑1 (MQ=255)

                                                                                                                    tttCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAAc         <  1:1495548/127‑1 (MQ=255)

                                                                                                                    tttCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAAc         >  2:1495548/1‑127 (MQ=255)

                                                                                                                    tttCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACg        <  1:2757666/128‑1 (MQ=255)

                                                                                                                     ttCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACGa       <  1:1569130/128‑1 (MQ=255)

                                                                                                                       cagcagATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACGatt     >  1:4146501/1‑128 (MQ=255)

                                                                                                                       cagcagATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACGatt     >  2:157027/1‑128 (MQ=255)

                                                                                                                       cagcagATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACGatt     >  2:2507867/1‑128 (MQ=255)

AAGAACTTGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGGTTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACGATTAAT  >  CP016816/242732‑242981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`