Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816202,4960A.73.6% 391.5 / inf 295intergenic (+274/‑401)JCVSYN2_00840/JCVSYN2_00845hypothetical protein/lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (15/15);  major base . (5/190);  minor base T (18/52);  total (38/257)
Fisher's exact test for biased strand distribution p-value = 7.08e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.37e-02

Only 100 of 538 total aligned reads displayed.

ATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTT‑CACAAAT‑AAAAAAAAAACAATCCCCTTTTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATACTATATTGCTATG  >  CP016816/202370‑202624
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ataataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTT‑CACAAAT‑AAAAAAAAAACAATCCAC‑‑Ttaaaaattaaaa                                                                                                                                >  1:1581529/1‑128 (MQ=255)
ataataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTT‑CACAAAT‑AAAAAAAAAAAAAACCCAtt‑‑‑aaaaataaaaa                                                                                                                               >  1:2384500/1‑128 (MQ=255)
   ataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTAT‑ATGTAGATCCTCGAGATCTATTTTATTATTTTTTT‑ATCTT‑CACAAAT‑AAAAAAAAAAAAAAACC‑‑AATAAAAAATaaaaaaaa                                                                                                                            >  2:3946696/1‑128 (MQ=255)
    taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTT‑CACAAATAAAAAAAAAAACAAACCCCTT‑‑‑AAAAATaaaaaaaa                                                                                                                            >  1:4192471/1‑128 (MQ=255)
    taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTT‑CACAAAT‑AAAAAAAAAACAAACCCC‑‑TTAAAAATTaaaaaaaa                                                                                                                            >  1:3717619/1‑128 (MQ=255)
    taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTATTATAATATATATCCTCTTGATCTAAATAAATATTTGATT‑ATCTT‑CACAAAT‑AAAAAAAAAACAATCAAC‑‑‑TAAAAAATaaaaaaaaa                                                                                                                           >  2:1781205/1‑128 (MQ=255)
     agagTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTTTTT‑ATATT‑CACAAAT‑AAAAAAAAAAAAATCAC‑‑‑ATAAAAAATaaaaaaaaaa                                                                                                                          >  2:344408/1‑128 (MQ=255)
     agagTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTAATTTTTTATTTTTTT‑ATCTT‑CACAAAT‑AAAAAAAAAACAAAACCC‑‑TTAAAAATTaaaaaaaaa                                                                                                                           >  1:353619/1‑128 (MQ=255)
       atttAAGGTTAATTAAAATACAATAAAATTAAAAAATATTAAAAATAATAACCTCGTGTACTATTTTTTTATTTTTTT‑ATAAT‑CACAAAA‑AAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaa                                                                                                                         <  2:2085019/126‑1 (MQ=255)
        gTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTT‑CACAAAT‑AAAAAAAAAAAAATCCAC‑‑‑TAAAAAATtaaaaaaaaaaaa                                                                                                                       >  1:1516758/1‑128 (MQ=255)
         ttAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTTTTT‑ATCTT‑CACAAAT‑AAAAAAAAAACAAACCCAATaaaaaaaaaaaaaaaaaaaa                                                                                                                         >  2:4297503/1‑128 (MQ=255)
          tAAGGTTAATTAAAATACCATAAACATAATAATTATTAAAATGTAGATCCTCGAGATCTATATTTTTATTTGTTT‑ATCTT‑CACAAAT‑AAAAAAAAAACAATCCAC‑‑TTAAAAATTaaaaaaaaaaaata                                                                                                                      >  1:754399/1‑126 (MQ=255)
           aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTT‑CACAAAT‑AAAAAAAAAACAATCC‑‑‑AATAAAAAATtaaaaaaaaaaaaaaa                                                                                                                    >  1:1387722/1‑128 (MQ=255)
           aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTCATTTGTTT‑ATCTT‑CACAAAT‑AAAAAAAAAACAATCCCC‑‑TTAAAAAATaaaaaaaaaaaaaaa                                                                                                                     >  1:468665/1‑128 (MQ=255)
            aGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTT‑CACAAAT‑AAAAAAAAAACAATCCAC‑‑TTAAAAATTaaaaaaaaaaaaaaaa                                                                                                                    >  1:2322825/1‑128 (MQ=255)
             ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTT‑CACAAAT‑AAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaa                                                                                                                   >  2:4079834/1‑128 (MQ=255)
             ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTT‑CACAAAT‑AAAAAAAAAACAAACCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaa                                                                                                                   >  1:4303933/1‑128 (MQ=255)
             ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTT‑CACAAAT‑AAAAAAAAAAAAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaa                                                                                                                   >  2:3579041/1‑128 (MQ=255)
             ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATACTCTTGATCTATTTTTTTATTTGTTT‑ATCTT‑AACAAAT‑AAAAAAAAAACAAAAAC‑‑‑AAAAAAAATtaaaaaaaaaaaaaaaaa                                                                                                                  >  2:1676735/1‑128 (MQ=255)
             cgTTAAATAAAATAACTTAATATTAAAAATTATTAAAATTTAAAACATTGTTATATATTTTTTTATTTTTTA‑AAATA‑CAAAAAT‑AAAAAAATAAAAATACCC‑‑‑TAAAAAATtaaaaaaaaaaaaaaaaa                                                                                                                  <  2:3359781/127‑1 (MQ=255)
              gTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTT‑CACAAAT‑AAAAAAAAAACAAACCCC‑‑TTAAAAAATaaaaaaaaaaaaaaaaaa                                                                                                                  >  1:2357636/1‑128 (MQ=255)
               ttaattaaAAAACAATAAAATTAAATATTTTTTTAATTTATATACTTTTTTTTATTTTTTTTATTTTTTT‑ATTTT‑AAAAAAT‑AAAAAAAAAAAAAACCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaac                                                                                                                 <  1:3797956/128‑2 (MQ=255)
                taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTT‑CACAAAT‑AAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaa                                                                                                                >  1:2979564/1‑128 (MQ=255)
                taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTT‑CACAAAT‑AAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaa                                                                                                                >  2:3890043/1‑128 (MQ=255)
                taattaaAATACCATAAAAATATAAATTATTATAAAGTTTTACCTAGGGATCTATTTTTTTATTTGTTT‑ATCTT‑CACAAAA‑AAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaac                                                                                                                <  1:3557710/128‑2 (MQ=255)
                 aatttaAATAACATAAAATTAATAATTATTATAATTTATATCCTAGTTATATATTTTTTTATTTTTTT‑ATCTT‑CACAAAT‑AAAAAAAAAA‑AATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaactaaaa                                                                                                              <  2:4542778/128‑7 (MQ=255)
                 aattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTT‑CACAAAT‑AAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaa                                                                                                               >  1:3771777/1‑128 (MQ=255)
                  attaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTT‑CACAAAT‑AAAAAAAAAACAATCCAC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaaa                                                                                                              >  2:3502955/1‑127 (MQ=255)
                  attaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTT‑CACAAAT‑AAAAAAAAAACAATCACC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaaa                                                                                                              >  1:1302124/1‑127 (MQ=255)
                   ttaaAATACCATAAAATTAATAATTAATAAAATTTATATACAAGTTATCTATTTTTTTATTTTTTT‑ATATT‑CAAAAAT‑AAAAAAAAAAAAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaactaa                                                                                                             <  2:1229369/128‑5 (MQ=255)
                   tataaataaaataAAATTATTAATTTTTATAATTTATATAATTGTTTTTTATTTTTTTTTTTTTTT‑ATATT‑AAAAAAA‑AAAAAAAAAAAAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaacaga                                                                                                             <  2:2613618/125‑5 (MQ=255)
                     aaaaTACCATAAACTTAATAATTCTAATAATGTAGATACTCGTTATCTATTTTTTTATTTGTTT‑ATCTT‑CACAAAT‑AAAAAAAAAACAAAACCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaacaaaaa                                                                                                           >  1:1946110/1‑122 (MQ=255)
                      aaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTT‑CACAAAT‑AAAAAAAAAAAAATCAC‑‑ATTAAAAATTaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                                          >  2:548283/1‑123 (MQ=255)
                       aaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTT‑CACAAAT‑AAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAATaaaaaaaa                                                                                                         >  2:3650861/1‑128 (MQ=255)
                        aaaacATAAAATTAAAAATTCTTATAATGTATTTCCTGGTTATATATTTTTTTATTTTTTT‑ATATT‑CACAAAT‑AAAAAAAAAACAATCCCCTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAaaaca                                                                                                      <  2:1645521/124‑1 (MQ=255)
                        aTAACATAAAATTAATAATTATTATAATTTATATACTTTTGATATATTTTTTTTTTTGTTT‑TTTTT‑AAAAAAT‑AAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAaaac                                                                                                       <  1:3398008/128‑1 (MQ=255)
                         tACCATAAACTTAATAATTCTTATAATGTAGATCCTAGTTATCTAATTTTTTATTTTTTT‑ATCTT‑CACAAAT‑AAAAAAAAAACAATCCCC‑‑TAAAAAATTaaaaaaaaaaaaaaaaaaaaaaaaacaca                                                                                                       >  1:4150860/1‑120 (MQ=255)
                          aacttAAAATTTATAATTCTTATAATGTAGATAATAGTTATATATTTTTTTATTTGTTT‑ATATT‑AAAAAAT‑AAAAAAAAAAAAATCCCCTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAaaacaaa                                                                                                    <  2:1003330/124‑1 (MQ=255)
                              tAAAATTAAAAATTCTAATAATGTACAACCTCGTGATCAATTTTTTTATTTGTTT‑AACTT‑AACAAAT‑AAAAAAAAAACAATCCCCAT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAActct                                                                                                <  2:868899/128‑1 (MQ=255)
                              aaaaaTTAATAATTTTTATAAATTATATCCTCGTGATATATTTTTTTATTTTTTT‑ATATT‑CACAAAT‑AAAAAAAAAACAAACCCCTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAActct                                                                                                <  2:4395627/127‑1 (MQ=255)
                               aaaCTTAATAATTATTATATTGTATTTACTTGTTTTATATTTTTTTATTTTTTT‑AACTT‑AAAAAAT‑AAAAAAAAAAAAAACCCC‑‑TTAAAAAAT‑AAAAAAAAAAAAAAAAAAAACTAAAAACAAActct                                                                                                <  1:3934040/128‑1 (MQ=255)
                                aaCTTTATAATTTTTATAATTTAGATACAAGTTATTTATTTTTTTATTTTTTT‑ATATT‑AAAAAAT‑AAAAAAAAAAAAATCCCCTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTAt                                                                                              <  2:4128518/128‑1 (MQ=255)
                                 aatttaaaatTTTTATATTTTTGTTCCTCGTTTTTTTTTTTTTTATTTTTTT‑TTATT‑AAAAAAA‑AAAAAAAAAACAAATCCCTT‑‑‑AAAAATTTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTAt                                                                                              <  1:3006068/121‑1 (MQ=255)
                                 aCTTTATAATTAATAAAATTTATATAATAGTTATCTATTTTTTTAATTTTTT‑ATATT‑CACAAAA‑AAAAAAAAAAAAAAACCCCAT‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTAt                                                                                              <  2:491437/128‑1 (MQ=255)
                                   ttattaATTCTTATAATGTTTTTCCTTTTGATTTTTTTTTTTATTTGTTT‑ATCTT‑CACAAAT‑AAAAAAAAAAAAATCCCCTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTc                                                                                           <  2:3778651/128‑1 (MQ=255)
                                   ttAATAAATCTTAAAATGTAGATCCTCGTTATTTATTTTTTTATTTTTTT‑AAATT‑CCAAAAT‑AAAAAAAAAAAAATACCCTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTc                                                                                           <  2:4212074/128‑1 (MQ=255)
                                       ttatTCTTAAAATTTATATACAAGTTATATAATTTTTTATTTTTTT‑AAATT‑CAAAAAA‑AAAAAAAAAAAAATCCCCTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTaa                                                                                       <  2:300166/126‑1 (MQ=255)
                                        aatTTTTATAATGTATATCCTAGTGATATATTTTTTTATTTGTTT‑ATCTT‑CACAAAT‑AAAAAAAAAACAATCCCCTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTaaa                                                                                      <  2:2260658/128‑1 (MQ=255)
                                                 aaTGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTT‑CACAAAT‑AAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAA‑TAAAAAACAACTATATTTGATAAAAATGCCttt                                                                             >  1:1167506/1‑126 (MQ=255)
                                                   attttataatCGTTATTTTTTTTTTTATTTTTTT‑ATTTT‑TAAAAAA‑AAAAAAAAAAAAATACCCTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtatt‑a                                                                          <  1:4540869/119‑1 (MQ=255)
                                                      tcttCCTCGTGATGTTTTTTTTTTTTTGTTT‑ATATT‑CACAAAA‑AAAAAAAAAAAAATCCCCTT‑‑‑AAAAAT‑‑‑TAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACca                                                                     <  1:4432224/125‑1 (MQ=255)
                                                        atactattGATATATTTTTTTATTTGTTT‑ATAAT‑AACAAAT‑AAAAAAAAAAAAAACCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATAcc                                                                      <  2:4496865/121‑1 (MQ=255)
                                                        aaaatatttttttatttttttattttttT‑ATATT‑TAAAAAT‑AAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATAcc                                                                      <  2:2930619/116‑1 (MQ=255)
                                                          tatagtttttatttttttttttttttT‑ATATT‑AAAAAAA‑AAAAAAAAAAAAATACACTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACcac                                                                    <  1:1012030/116‑1 (MQ=255)
                                                          actagttatatatttttttattttttT‑ATATT‑AAAAAAA‑AAAAAAAAAAAAAATCCC‑CTTAAAAAAT‑‑AAAAAAAAAAAAAAAAAAACTAAAAACAAATTCTATTTCTTAAAATTCCCTATT‑ATACcac                                                                    <  2:3711479/118‑1 (MQ=255)
                                                             tgtgaatattttttttAATTTTTT‑ATTAT‑AACAAAT‑AAAAAAAAAAAAAAACCC‑‑ATAAAAAAT‑‑AAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACattt                                                                <  2:3689263/119‑1 (MQ=255)
                                                             ctttaTCAATTTTTTTATTTTTTT‑ATCTT‑AAAAAAT‑AAAAAAAAAAAAATCCCCAT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACattt                                                                <  2:2797808/124‑1 (MQ=255)
                                                             cGTGATTTATTTTTTTATTTGTTT‑ATCTT‑AACAAAT‑AAAAAAAAAACAATCCCATT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACatttt                                                               <  2:3662396/128‑1 (MQ=255)
                                                                 atatttttttttATTTTTAT‑AAATT‑AAAAAAA‑AAAAAAAAAAAAATCCC‑‑‑ATAAAAAAT‑‑‑AAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACattttatttt                                                          <  2:1292978/123‑1 (MQ=255)
                                                                 atatATTTTTTTTTTTTTTT‑ATATT‑AAAAAAT‑TAAAAAAAAAAAATACCCAT‑‑‑AAAAAT‑‑‑TAAAAAAAAAAAAAAAAACTAACAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACattttatttt                                                          <  2:3549793/125‑1 (MQ=255)
                                                                   aattttttttattttttt‑aTATT‑AAAAAAA‑AAAAAAAAAAAAATACCCTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACattttatttt                                                          <  1:1149412/125‑1 (MQ=255)
                                                                     tattttttATTTGTTT‑ATATT‑AAAAAAA‑AAAAAAAAAAAAAATCCCTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTa                                                        <  1:1094247/126‑1 (MQ=255)
                                                                     atttttttattttttt‑AAAAT‑AAAAAAA‑AAAAAAAAAAACAAACCCAT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTAttttt                                                         <  2:192376/128‑1 (MQ=255)
                                                                     aattttttATTTTTTTAATTTT‑AAAAAAA‑AAAAAAAAAACAATCCCCTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTAttttt                                                         <  1:1663211/126‑1 (MQ=255)
                                                                      attttttattttttt‑atAAT‑AAAAAAA‑AAAAAAAAAAAAATACCC‑‑ATAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTAttttt                                                         <  2:663911/127‑1 (MQ=255)
                                                                       ttttttttttttTA‑TTATT‑AAAAAAT‑AAAAAAAAAAATATCACCTT‑‑‑AAAAAT‑AAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTaaa                                                      <  1:3695390/128‑1 (MQ=255)
                                                                       ttttttattttttt‑atTTA‑AAAAAAT‑AAAAAAAAAAAAATACCC‑‑TTAAAAAAT‑AAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTaa                                                       <  2:4185246/128‑1 (MQ=255)
                                                                       ttttttATTTGTTT‑ATCTT‑CACAAAT‑AAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAA‑TAAAAAAAAAATTAATTTATT‑AAATTACAAATTAAAACAAAATTTTATTTTTaa                                                       >  1:3992599/1‑128 (MQ=255)
                                                                       ttttttATT‑‑TTT‑ATATT‑AAAAAAA‑AAAAAAAAAACAATCCCCTT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATa                                                   <  1:2441229/128‑1 (MQ=255)
                                                                        ttttttttTGTTT‑TTTTTATATAAAA‑AAAAAAAAAACAAGCCC‑‑‑TT‑AAAAATTAAAAAAAAAACAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTaaa                                                      <  2:2325103/128‑1 (MQ=255)
                                                                          tttATTTTTTT‑TTTTT‑AACAAAA‑AAAAAAAAAAAAATCCCATT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATa                                                   <  1:4401669/128‑1 (MQ=255)
                                                                          tttATTTTTTT‑ATATT‑AAAAAAT‑AAAAAAAAAAAAATACCCTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATa                                                   <  2:154262/128‑1 (MQ=255)
                                                                           ttATTTTTTT‑ATATT‑TAAAAAT‑AAAAAAAAAAAAATACCCTT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAACAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAg                                                 <  2:3252203/128‑1 (MQ=255)
                                                                           ttATTTTTTT‑AAATT‑AAAAAAT‑AAAAAAAAAAAAAT‑‑AAAATTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAAt                                                    <  2:4019490/128‑1 (MQ=255)
                                                                             aTTTTTTT‑ATCTT‑AAAAAAT‑AAAAAAAAAACAATCCCCTT‑‑‑AAAAAT‑‑TTAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAGat                                               <  2:2546787/128‑1 (MQ=255)
                                                                             aTTTGTTT‑ATCTT‑CACAAAT‑AAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAA‑‑AAAAACAAACTAAATTTATAAAAATTCCCTATT‑AAACCAATTTTTTTTTTTAAAAAAAAat                                               >  1:3424900/1‑128 (MQ=255)
                                                                               tttttt‑ATATT‑AAAAAAT‑AAAAAAAAAAAAATACCCTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAGata                                              <  2:2146006/125‑1 (MQ=255)
                                                                               tatttataaatt‑CACAAA‑‑AAAAAAAAAAAAATAACCAATT‑AAAAAT‑‑AAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTATTAAAATTCCCTATA‑ATACCACATTTTATTTTTAAAATAAGaa                                               <  2:768797/118‑2 (MQ=255)
                                                                                   tttataaataaaaaaa‑aaaaaaaaaaCAATAACC‑‑TTAAAAAAT‑‑AAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAGATAtt                                            <  2:2848850/116‑1 (MQ=255)
                                                                                   tt‑ATCTT‑CACAAAT‑AAAAAAAAAACAATCCCCTT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACTATTTTTATTAAAATTCACTATT‑ATACAACATTTTATTTTTAAAATATGTAATTGAc                                         >  2:2414479/1‑128 (MQ=255)
                                                                                     aatataaaaaaaaa‑aaaaaaaaaaaaATACCATT‑‑‑AAAAATT‑AAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAGATATTGa                                          <  2:4445111/118‑1 (MQ=255)
                                                                                      tattataaaaaaT‑AAAAAAAAAACAAAACCCTT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAGATATTGACa                                        <  1:4259520/119‑1 (MQ=255)
                                                                                      taatttaaaaaaT‑AAAAAAAAAACAATCCAATT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACAAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAGATATTGACa                                        <  1:3507974/119‑1 (MQ=255)
                                                                                       aaatatacaAAA‑AAAAAAAAAAAAAAACCC‑‑ATAAAAAAT‑AAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAGATATTGAc                                         <  2:2122535/122‑1 (MQ=255)
                                                                                        tattactatct‑AAAAAAAAAACAATCCCCTT‑‑‑AAAAAT‑‑‑TAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAGATATTGACataa                                     <  2:3399438/118‑1 (MQ=255)
                                                                                          ataaaaaaa‑aaaaaaaaaaCAATCCCCTT‑‑‑AAAAAT‑AAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAGATATTGACataa                                     <  2:3974733/123‑1 (MQ=255)
                                                                                              aaaaT‑AAAAAAAAAAAAATACCCTT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAGATATTGACATAatatat                                <  2:145685/127‑1 (MQ=255)
                                                                                               aaaT‑AAAAAAAAAAAAATACCATT‑‑‑AAAAATT‑‑AAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATAACACATTTTATTTTTAAAATAAGATATTGACATAatatata                               <  1:17349/128‑1 (MQ=255)
                                                                                                 aT‑AAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAA‑‑AAAAACAACCAATATTGATTAAAAAAACCTATA‑AAAACAAAATTTATTTTTAAAAAAAAAAATTGACATAaaatcaaa                              >  1:285181/1‑124 (MQ=255)
                                                                                                  aaaaaaaaaaaaaaaaACCC‑‑TTAAAAAAT‑‑AAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAGATATTGACATAatatatat                              <  2:3821220/126‑1 (MQ=255)
                                                                                                                 caatccccttaaaaattaaaaaAAAAAAAAAAAAAAAATAAAAACAAAAAATATTTATTAAAATAACAATTT‑ATAAAAAATTTTATTTTAAAAAAAATATATTTAAATAATATTTATAAAatatatat                   >  1:2117417/11‑128 (MQ=255)
                                                                                                                  aaaaacccttaaaaaataaaaAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTAAT‑ATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAAtatatata                  <  1:3869832/118‑1 (MQ=255)
                                                                                                                      aacttaaaaattaaaaaAAAAAAAAAAACAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATAc              <  2:3469890/123‑1 (MQ=255)
                                                                                                                        cttaaaaattaaaaaAAAAAAAAAAAAAAA‑TAAAAAAAAAGTATATTTCTTAAAATTCCATATT‑TAACCCAATTTTATTTTTAAAAAAAGATATTGAAAAAATAAATATATAATATATAAAAAAatat           >  1:4058185/4‑128 (MQ=255)
                                                                                                                        cttaaaaattaaaaaAAAAAAAAAAAAAAA‑‑AAAACCAAACTCTTTTTATTAAAATTCCATATT‑AAACCAAATTTTTTTTTTAAAATTATTTTTTTACTAAATTTATATTAAATATATAAAataataat          >  2:4369756/4‑126 (MQ=255)
                                                                                                                         taaaaaat‑taaaaaAAAAAAAAAAAAGACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATACtata          <  1:3651072/128‑1 (MQ=255)
                                                                                                                          ttaaaaaataaaaAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATAGAACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATACtata          <  1:4094770/126‑1 (MQ=255)
                                                                                                                           aaaaataaaaaaAAAAAAAAAAAAAA‑‑TAAAAACAAACTCTATTTCTTAAAATTCACTATT‑ATAACACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATACTATATTg       <  2:1079633/128‑1 (MQ=255)
                                                                                                                              aaaaaaaaaaaaaaaaaaaaaaaaCTAAAAACAAACTCTATTTCTTAAAATTACCTATT‑ATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATACTATATTGc      <  2:3736390/125‑1 (MQ=255)
                                                                                                                                taaaaaaAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCACTATT‑ATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATACTATATTGCTa    <  2:1139944/128‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                 
ATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTT‑CACAAAT‑AAAAAAAAAACAATCCCCTTTTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATACTATATTGCTATG  >  CP016816/202370‑202624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑