Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2500T.59.8% 479.7 / inf 1283intergenic (+214/‑328)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base T (310/187);  new base . (559/179);  total (876/407)
Fisher's exact test for biased strand distribution p-value = 6.65e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1575 total aligned reads displayed.

ATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGC‑AAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGC‑T‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTT‑TGTTGT‑CTAAGTATCT‑AAAAAAAT‑AGAAAG‑GAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAAC  >  CP016816/165123‑165377
                                                                                                                                  |                                                                                                                                     
ataataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑t                                                                                                                                       >  2:187838/1‑128 (MQ=255)
  aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑t‑tt                                                                                                                                    >  2:677360/1‑128 (MQ=255)
  aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑t‑tt                                                                                                                                    >  2:4431142/1‑128 (MQ=255)
   atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTTAACTTGTATAAAATAGA‑t‑ttt                                                                                                                                   >  1:2926352/1‑128 (MQ=255)
   atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑t‑ttt                                                                                                                                   >  1:704665/1‑128 (MQ=255)
   atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑t‑ttt                                                                                                                                   >  1:1653155/1‑128 (MQ=255)
    tatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑t‑tttt                                                                                                                                  >  2:131275/1‑128 (MQ=255)
    tatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑t‑tttt                                                                                                                                  >  2:2171388/1‑128 (MQ=255)
        tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGATACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGA‑t‑ttttttat                                                                                                                              >  2:2971713/1‑126 (MQ=255)
          gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑t‑tttttttttt                                                                                                                            >  1:127218/1‑128 (MQ=255)
            ttCAATAATTTATCGCAATTTTATTAATTTGTAATAAAATAAAAATTTTATTTTTTTATA‑TATTTTTTGTATATGC‑AAATTTTTTAAAAATTAATTTTAAATTTTAAAAAATATC‑t‑tttttttttttt                                                                                                                          <  1:1740829/128‑1 (MQ=255)
             tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑t‑ttttttttttttt                                                                                                                         >  1:4387862/1‑128 (MQ=255)
                   aTTTAACCCACTTTTATGAATTACTAATAAACAAAAAAAAATATTATTTTATT‑TATTTTTTTTATATTG‑AAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATACC‑T‑TTTTTTTTTTTTTTTTTta                                                                                                                   <  2:346202/128‑1 (MQ=255)
                    tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑t‑ttttttttttttttttttta                                                                                                                  >  2:572278/1‑126 (MQ=255)
                    tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGC‑AAATATTTAAATAATTAATTTGAACTTGTAAAAAATAGC‑t‑tttttttttttttttttata                                                                                                                  >  1:3469858/1‑125 (MQ=255)
                    tttATAGCAATTTTATTTATTATTTATAAACTAAAACTATTATCATTTTATA‑TAATTTTTTTTTATGC‑AAAATTTTAAAAAATTAAATTTAACATTTAAAAAATATC‑T‑TTTTTTTTTTTTTTTtataa                                                                                                                  <  2:4200087/128‑2 (MQ=255)
                         cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑T‑TTTTTTTTTTTTTTTTTtatttgaa                                                                                                             >  1:4274937/1‑123 (MQ=255)
                         cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTTATAATGGa                                                                                                            >  2:3653573/1‑128 (MQ=255)
                              ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑T‑TTTTTTTTTTTTTTTTTAATAATGaaaaaa                                                                                                        >  1:1340140/1‑126 (MQ=255)
                              ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTTATAATTAAAatat                                                                                                       >  2:1241273/1‑128 (MQ=255)
                                 tttttatttttaataaAATAAAAATATTATATTTTTTTA‑TATTTTTTTTTTATTC‑AAATTTTTTAAAAATTTATTTTTAATTTTTAAAAATATC‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATaaa                                                                                                    <  2:4500865/118‑1 (MQ=255)
                                   ttaataatttataaaataaaaataTTATCTTTTTTTA‑TATTTTTTTTATATTC‑AAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATATC‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                                                  <  1:927633/116‑1 (MQ=255)
                                   tGAAATATTAATAAAATAATAATAATTTATTTTTATT‑TATTTTTTTTAATTGA‑TAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATATC‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                                                  <  2:2005643/128‑1 (MQ=255)
                                   agAATTATTAACTAAAAAAAAAAATTTTTTTTTTTTA‑TATTTTTTTTTTATTC‑AAATTTTTAAAAAATTAAATTTAAAATTTAAAAAAAATC‑‑‑ATTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                                                  <  2:1082752/127‑1 (MQ=255)
                                    ttattattAATAAAAAAAAAATATTTTTTTTTTATA‑TATTTTTTTTATATTCAAAATTTTAAAAAAATAAATTT‑AAATTTTAAAAAAAATC‑T‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                                                  <  2:507270/126‑1 (MQ=255)
                                      aTTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATC‑‑‑TTTTTTTTTTTTATTTTTAAATTAAAAATATAAATTTTa                                                                                               >  2:908814/1‑128 (MQ=255)
                                       ttattaATAAAATAAAAATATTATCTTTTTATA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTTTAAAAAATAGC‑‑‑‑‑‑‑TTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAt                                                                                          <  2:2799976/128‑1 (MQ=255)
                                         atttattaaaaaaaaataATATTTTTTTTTA‑TATTTTTTTTTTTTTT‑AAATTTTTAAAAAATTAAATTTAAATTTTAAAAAATATC‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                                                            <  2:3409858/117‑1 (MQ=255)
                                         attaataaAATAAAAATTTTTTATTTTTATT‑AATTTTTTTTATATGA‑AAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATAGC‑T‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAgg                                                                                             <  2:3131511/126‑1 (MQ=255)
                                          gTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTTATATTGTAAATATAAAtttttattt                                                                                           >  2:1148454/1‑123 (MQ=255)
                                            ataaaattaaaattaTT‑TTTTTTTTTA‑TTTTTTTTTTTTATTT‑AAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATA‑‑‑T‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAttt‑t                                                                                      <  1:1402300/125‑1 (MQ=255)
                                              gAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTTAAAATTAAAATAAAAATTTAATTTAttt‑t                                                                                      >  1:210569/1‑128 (MQ=255)
                                              aaaaattaaaaaattatATTTTTATT‑TATTTTTTGTATTTGG‑AAATTTTTAAAAAAATAATTTTTACTTGTTAAAATTAGC‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAttt‑t                                                                                      <  2:2628423/116‑1 (MQ=255)
                                               aaactaaaactaTTATCTTTTTAGA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑T‑TTTTTTTTTTTTTTTTTTATAAAGTAAATATAAATTTTATGTAttt‑t                                                                                      >  1:1492331/1‑128 (MQ=255)
                                                  attaaaataTTATTTTTTTATATTTTTTTTTTTATTTTT‑AATTTTTTAAAAAATTATTTTTAA‑TTTTAAAAAATTTG‑T‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTt‑tgtt                                                                                   <  2:1382519/125‑1 (MQ=255)
                                                  ataaaaataTTTTTTTTTTATT‑TATTTTTTTTATATTT‑AAATTTTTAAAAAATTAATTTTTAATTTTAAAAAAATTT‑T‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTt‑tgtt                                                                                   <  2:16525/127‑1 (MQ=255)
                                                   taaaactaTTATCTTTTTAGA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑T‑TTTTTTTTTTTTTTTTTTA‑AATTTAAAAATAAATTTTAGTTATtt‑tttttt                                                                                 >  1:1156128/1‑126 (MQ=255)
                                                    aaacctaTTATCTTTTTAGA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑T‑TTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGTAATtt‑tttttt                                                                                 >  2:332103/1‑126 (MQ=255)
                                                     aaactaTTATCTTTTTAGA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTTATAATGTAAAAATAAATTTTATTTATtt‑ttttttat                                                                               >  1:2550630/1‑124 (MQ=255)
                                                     aaactaTTATCTTTTTAGA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTTAAAATGTAATTATAATTTTTAGTTATTT‑TTTTGT‑CTa                                                                             >  1:3617131/1‑128 (MQ=255)
                                                        cttttatATTTTTATA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTGTACTTGTAAAAAATATC‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTT‑TGTTGT‑CTAAg                                                                           <  2:3533788/125‑1 (MQ=255)
                                                             atCTTTTTAGA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTTATATTGTAATAATAAATTTATTGTATtt‑ttttttaaaattatat                                                                       >  1:4567564/1‑116 (MQ=255)
                                                              tCTTTTTAGA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TATTTTTTTTTTTTTTTTTTTTAATGTAAATATAAATTTTATGTTTTT‑TGTTTT‑ATAATTAta                                                                       >  1:4180540/1‑127 (MQ=255)
                                                                tttttAGA‑TATTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTTATAAGTTATAAATAATTTTTAGTAATTT‑TTTTGT‑Ataaataaat‑aaa                                                                  >  1:4017812/1‑128 (MQ=255)
                                                                    ttttttatTTTTTGTATTTTC‑AAATTTTTAAAAAATTTATTTTAACTTGTAAAAAATA‑‑‑T‑CTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTT‑TGTTGT‑CTAAGTATCT‑aaaaaaa                                                              <  2:2479419/123‑1 (MQ=255)
                                                                    ttttattttttttttttATTT‑AAATTTTTAAAAAATTAATTTTTAATTTTAAAAAA‑ATT‑T‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTT‑TGTTGT‑CTAAGTATCT‑aaaaaa                                                               <  2:1948971/121‑1 (MQ=255)
                                                                      ttttatTTTTTGTTTTTTT‑TAATTTTTTAAAAATTTATTTTTACTTTTAAAAAAAA‑‑‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTT‑TGTTGT‑CTAAGTATCT‑AAAAAAAT‑Ag                                                          <  2:2305949/125‑1 (MQ=255)
                                                                       tttatTTTTTTTATATGA‑AATTTTTAAAATAATTAATTTTATATTTTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTT‑TGTTGT‑CTAAGTATCT‑AAAAAAAT‑Ag                                                          <  1:2469763/126‑1 (MQ=255)
                                                                       a‑tatTTTTTGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAATAAAAATTTAATTTATTT‑TTTTTT‑ATAATTATAT‑AAAAAAAT‑aaa                                                         >  1:1603181/1‑126 (MQ=255)
                                                                          atTTTTTTTTTATTT‑AAATTTTTTATAAATTAATTTGAATTTGTAAATAAT‑‑‑‑T‑TTTTTTTTTATTTTTTTTATAATTGAAATATAAATTTTAGGTATTT‑TGTTGT‑CTAAGTATCT‑AAAAAAAT‑AGAAAg                                                      <  2:1004172/128‑1 (MQ=255)
                                                                             ttttGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑T‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTTGTTATTT‑TTTTTT‑CTAATTATAT‑AAAAAAAT‑AGAAAg                                                      >  1:572728/1‑128 (MQ=255)
                                                                              ttagatatttc‑AAATTTTTTAAAAATTAATTTTTAATTTTTAAAAATA‑‑‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTT‑TGTTGT‑CTAAGTATCT‑AAAAAAAT‑AGAAAG‑Gaaa                                                 <  1:2731679/118‑1 (MQ=255)
                                                                                tGTATATGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTTATAATTAAAATAAAATTTTTAGTTTTTT‑TTTTTT‑ATATTTATAA‑AAAAAAAA‑AAAAAG‑Taaa                                                 >  1:1003325/1‑128 (MQ=255)
                                                                                 tttttatttaatttttAAAAAATTTATTTTTAAATT‑TAAAAAAAATT‑T‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTT‑TGTTGT‑CTAAGTATCT‑AAAAAAAT‑AGAAAG‑Gaaa                                                 <  1:2397870/116‑1 (MQ=255)
                                                                                   atatTC‑AAATTTTTAAAAAAATAATTTTAAATTGTAAAAAATATC‑T‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTT‑TGTTGT‑CTAAGTATCT‑AAAAAAAT‑AGAAAG‑Gaaaaa                                               <  2:3735551/128‑1 (MQ=255)
                                                                                    tatGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑T‑TTTTTTTTTTTTTTTTTTA‑ATTTTAAAATTAAATTTTAGTTTTTT‑TGTTTT‑ATAAGTATAT‑AAAATAAT‑AAAAAT‑TAAAAAat                                             >  1:3731751/1‑128 (MQ=255)
                                                                                     atGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATGAAAATATAAATTTTAGTTATTT‑TTTTGT‑ATATTTTTAT‑AAAAAAAA‑ATAAAG‑AAAAAAaaat                                           >  1:2839950/1‑128 (MQ=255)
                                                                                     aaattaaaTTTTTAAAAAAATAATTTTTACATGT‑AAAAATAAT‑C‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTT‑TGTTGT‑CTAAGTATCT‑AAAAAAAT‑AGAAAG‑GAAAAAat                                             <  1:4092634/123‑1 (MQ=255)
                                                                                      tGC‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTT‑TTTTTTTTTTTTTTTTTTTTAATGGAAATATAAATTTTTGGTATTT‑TTTTTT‑ATAATTTAAT‑AAAAAAAT‑AAAAAG‑GAAAAAat                                             >  1:3793495/1‑128 (MQ=255)
                                                                                        c‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTT‑TTTTTT‑ATATGTATAT‑AAAAAAAT‑AGAAAG‑GAAAAAATAttt                                         >  2:4284248/1‑127 (MQ=255)
                                                                                        c‑AAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTTAAAATTAAAATAAAAATTTTAGTTTTTT‑TTTTGT‑ATAATTAAAT‑AAAAAAAA‑AAAAAA‑AAAAAAAttttt                                         >  1:1872906/1‑127 (MQ=255)
                                                                                           aaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATGGAATTATAATTTTTAGGAATTT‑TTTTTT‑ATAATTTTAT‑AAAAAAAT‑AGAATT‑GAAAAAATATTGttt                                      >  2:4353561/1‑127 (MQ=255)
                                                                                            aTTTTTAAAAAATTAATTTGAAATTTTTAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTT‑TGTTGT‑CTAAGTATCT‑AAAAAAAT‑AGAAAG‑GAAAAAATATTGTTa                                      <  2:3976941/128‑1 (MQ=255)
                                                                                             ttttttaaaaatttattTTTAATTTTAAAAAATATC‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTT‑TGTTGT‑CTAAGTATCT‑AAAAAAAT‑AGAAAG‑GAAAAAATATTGTTaa                                     <  1:3557913/119‑1 (MQ=255)
                                                                                                ttaaaaaaTTAATTTGAACTTGTAAAAAATAGC‑T‑TTTTTTTTTTTTTTTTTTTTAATGGTATAATAAATTTTAGTTTTTT‑TTTTTT‑TTAAGTATAT‑AAAAAAAT‑ATAAAG‑AAAAAATTATTTTTaaat                                   >  2:2889637/3‑127 (MQ=255)
                                                                                                ttaaaaaaTTAATTTGAACTTGTAAAAAATAGC‑T‑TTTTTTTTTTTTTTTTTT‑TAATGGAAATATAAATTTTATTTATTT‑TTTTTT‑ATAATTATAT‑AAAAAAAT‑AGAAAT‑TAAAAAATATTTTTaaaat                                  >  2:2119/3‑127 (MQ=255)
                                                                                                ttaaaaaaTTAATTTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAATTTTTAGTTATTT‑TTTTTT‑CTAATTATAT‑AAAAAATT‑AAAAAG‑TAAAAAATATTTTTAAAAGTa                                >  2:3593983/3‑128 (MQ=255)
                                                                                                 taaaaaaTTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATTT‑TGTTGT‑CTAATTATAT‑AAAAAAAT‑ATAAAG‑TAAAAAATATTTTTAAAAGt                                 >  2:4126032/2‑128 (MQ=255)
                                                                                                   aaaaatttattTTAAATTTTAAAAAATTTT‑T‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTT‑TGTTGT‑CTAAGTATCT‑AAAAAAAT‑AGAAAG‑GAAAAAATATTGTTAAAAGTa                                <  2:463995/125‑1 (MQ=255)
                                                                                                     aaattaattTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTTATAATGTAAATAAAAATTTTAGTTATTT‑TTTTTT‑ATAATTTTAT‑AAAAAAAT‑ATAAAG‑TAAAAAATATTTTTAAAAataata                             >  1:3840692/2‑128 (MQ=255)
                                                                                                      aattaattTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTTATATAGGAAATATAAATTTTAGTTATTT‑TATTTA‑AAAATTTACA‑AAAAAAAA‑AGAAAG‑TAAAAAAAATTTATAAAAGaaaaaa                            >  1:844532/1‑125 (MQ=255)
                                                                                                      aattaattTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTATGTATTT‑TTTTTT‑ATAATTATAA‑AAAAAAAA‑AAAATC‑TAAAAAATATTTTATAAATTAATAca                           >  1:2991019/1‑127 (MQ=255)
                                                                                                      aattaattTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTATTT‑TTTTTT‑ATAATTATAT‑AAAAAAAT‑ATAAAG‑TAAAAAATATTTTTaaaattgatctga                          >  2:2731679/1‑119 (MQ=255)
                                                                                                       attaattTGAACTTGTAAAAAATAGC‑T‑TTTTTTTTTTTTTTTTTT‑TAATGGAAATTTAATTTTTAGTTTTTT‑TTTTTT‑ATAATTATCT‑AAAAAAAT‑AAAAAG‑TAAAAAATATTTTTaaatttattaat                           >  2:3852275/1‑119 (MQ=255)
                                                                                                       attaattTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTTAAAATGAAAATA‑AAAATTTATGTATTT‑TTTTTTAATAATAATAA‑AAAAAAAT‑AAAATG‑TAAAAATTTTTTTTAAAATTaaaaaa                           >  1:110041/1‑124 (MQ=255)
                                                                                                       attaattTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTAAAAATGGAAATATAAATTTTAGGTATTT‑TGTTTG‑AAAATTAAAT‑AAAAAAAT‑ATAAAG‑TAAAAAATTTTTTTAAAATTAATaat                           >  1:2631927/1‑126 (MQ=255)
                                                                                                       attaattTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTT‑TGTTTT‑ATAATTATAT‑AAAAAAAT‑AGAAAG‑GAAAAAAAATTTTTAAAAGTAataata                          >  2:3902491/1‑128 (MQ=255)
                                                                                                       attaattTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGAATTT‑TTTTTT‑GAAAGTATAT‑AAAAAAAT‑ATAAAG‑GAAAAAAAATTGTTAAAAGTAAAACTa                          >  1:219075/1‑128 (MQ=255)
                                                                                                        ttaattTGAACTTGTAAAAAATAGC‑T‑TTTTTTTTTTTTTTTTTT‑TATTGAAAAAAAAAATTTTA‑GTTTTT‑TTTTTT‑TAAATTATATAAAAAAAAT‑AAAAAG‑AAAAAAATTTTTTTAAAATTAataata                          >  2:3793005/1‑128 (MQ=255)
                                                                                                        ttaattTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGTTTTTT‑TGTTTT‑ATAATTATAT‑AAAAAAAT‑AAAAAG‑TAAAAAATATTTTTTAAATTAataata                          >  2:2233162/1‑128 (MQ=255)
                                                                                                         taattTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATTT‑TGTTGT‑ATAATTATAT‑AAAAAAAT‑ATAAAG‑GAAAAAATATAGTTAAATGTATTACTaa                         >  2:737772/1‑128 (MQ=255)
                                                                                                         taattTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTT‑TTTTGT‑ATAAGTATAA‑AAAAAAAT‑AAAAAG‑AAAAAAATATTTTAAAAAGTAAAAATaa                         >  1:3374399/1‑128 (MQ=255)
                                                                                                            tttttatttttAAAAAATATC‑‑‑GTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTT‑TGTTGT‑CTAAGTATCT‑AAAAAAAT‑AGAAAG‑GAAAAAATATTGTTAAAAGTAATACTAAAat                      <  1:3671614/118‑1 (MQ=255)
                                                                                                               gAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTTATAATTTAAATAAAAATTTTAGGTATTT‑TTTTGT‑ATAATTTTAT‑AAAAAAAT‑ATAATG‑TAAAAAATATTTTTAAAATTAATaaaaaataatt                   >  2:1800335/1‑118 (MQ=255)
                                                                                                                 aCTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTTAAAATGTAAATATAAATTTTATTTTTTT‑TGTTTT‑ATAATTTTAT‑AAAAAAAA‑TTAAAG‑GAAAAAATATTTTTAAAATTATTAAAAAatttattt                 >  2:3026894/1‑128 (MQ=255)
                                                                                                                   ttGTAAAAAATTG‑‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTT‑TGTTGT‑CTAAGTATCT‑AAAAAAAT‑AGAAAG‑GAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAAt              <  2:2418297/128‑1 (MQ=255)
                                                                                                                     gTAAAATATAGC‑T‑TTTTTTTTTTTTTTTTTTA‑AATTGAAATAAAAATTTTAGGTATTTATTTTGT‑ATAATTTAAA‑AAAAAAAA‑AGAAAG‑TAAAAAATATTATTAAAAGTAATAATAAAATtaattaat              >  1:3239011/1‑128 (MQ=255)
                                                                                                                      tAAAAAATAGC‑T‑TTTTTTTTTTTTTTTTTTTTAATGTAATTATAAATTTTAGGTATTT‑TTTTTT‑ATAATTTTAT‑AAAAAAAT‑ATAAAG‑TAAAAAATATTTTTAAATTTAATAATAAAATTATTTAAtt             >  2:156819/1‑127 (MQ=255)
                                                                                                                      tAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTTATATTGGAAATATAAATTTTAGGTTTTT‑TGTTGT‑GTAATTATGT‑AAAAAAAT‑AGAAAG‑TAAAAAATATTTTTAAAATTAATAGTAAAATTATTTAATGt            >  2:1487802/1‑128 (MQ=255)
                                                                                                                      tAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATGGAATTATAAATTTTAGTTATTT‑TTTTTT‑ATAATTATAT‑AAAAAAAT‑ATAAAG‑TAAAAAATTTTTTTAAAATTAATAATAAAATAATTTAAtttt           >  2:3248421/1‑128 (MQ=255)
                                                                                                                       tttaaaaatttt‑ttttttttttttttttttATAATGGAAATATAAATTTTAGGTATTT‑TGTTGT‑CTAAGTATCT‑AAAAAAAT‑AGAAAG‑GAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATg             <  2:845212/117‑1 (MQ=255)
                                                                                                                         taaaaatttt‑ttttttttttttttttttATAATGTAAATATATATTTTAGGTATTT‑TGTTGT‑CTAAGTATCT‑AAAAAAAT‑AGAAAG‑GAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGtt           <  2:4038569/119‑1 (MQ=255)
                                                                                                                         aaaaTAGC‑‑‑TTTTTTTTTTTTTTTTTTAAAATGGAAATAAAAATTTTAGGTATTT‑TGTTTT‑ATAAGTAAAA‑AAAAAAAT‑AAAAAA‑AAAAAAATATTTTAAAAAATAAAAAAAAAATTATTTAATTttaa         >  1:49934/1‑128 (MQ=255)
                                                                                                                          aaaTAGC‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAAAATAAATTTTAGTTATTT‑TTTTTT‑GAAATTTTAA‑AAAAAAAT‑AAAAAG‑GAAAAAATATTTTTAAAATTAATAATAAAATTATTTATTTttaat        >  1:1969959/1‑128 (MQ=255)
                                                                                                                            aaatatt‑ttttttttttttttttttATAATGGAAATATAAATTTTAGGTATTT‑TGTTGT‑CTAAGTATCT‑AAAAAAAT‑AGAAAG‑GAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGttaat        <  1:3934070/122‑1 (MQ=255)
                                                                                                                            aTAGC‑‑‑TTTTTTTTTTTTTTTTTTATAATTTATAAATAAATTTTAGAAATTA‑TTTTGG‑AAAATTAAAA‑AAAAAAAT‑ATAATGAAAAAAAATATTATT‑AAATTAAAAAATAAATTATTAAATTttaataa      >  1:3112660/1‑126 (MQ=255)
                                                                                                                             aaatat‑ttttttttttttttttttATAATGTTAATATAAATTTTATTTATTT‑TGTTTA‑CTAATTATAT‑AAAAAAAT‑AGAAAG‑TAAAAAATATTTTTAAAAGTAATACTAAAATTATTTAATGttaatt       <  2:2553728/123‑1 (MQ=255)
                                                                                                                              atagt‑ttttttttttttttttttATAATGGAAATATAAATTTTAGGTATTT‑TGTTGT‑CTAAGTATAT‑AAAAAAAT‑ACAAAG‑GAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGttaatta      <  1:4480487/124‑1 (MQ=255)
                                                                                                                              aattt‑ttttttttttttttttttATAATGGAAATATAAATTTTAGGTATTT‑TGTTGT‑CTAAGTATCT‑AAAAAAAT‑AGAAAG‑GAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGttaatta      <  1:204543/124‑1 (MQ=255)
                                                                                                                                c‑T‑TTTTTTTTTTTTTTTTTTTTAATTAAAATAAAAATTTTATTTATTT‑TTTTTT‑C‑AAATAAAA‑AAAAAAATAAAAAAT‑AAAAAAATTTTTTTAAAATAAAAACTAAAATATTATATTTTTAATTaaaa   >  1:2294498/1‑128 (MQ=255)
                                                                                                                                  |                                                                                                                                     
ATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGC‑AAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGC‑T‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTT‑TGTTGT‑CTAAGTATCT‑AAAAAAAT‑AGAAAG‑GAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAAC  >  CP016816/165123‑165377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑