Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,222 G→A intergenic (+186/‑356) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2220GA98.9% 7777.8 / 51.6 2543intergenic (+186/‑356)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (1592/906);  minor base . (6/21);  total (1603/940)
Fisher's exact test for biased strand distribution p-value = 1.87e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2624 total aligned reads displayed.

CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAAT  >  CP016816/165097‑165349
                                                                                                                               |                                                                                                                                
cTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCTAATTTTTaaaaaa                                                                                                                                >  2:3405948/1‑128 (MQ=255)
cTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTaaaaaa                                                                                                                                >  1:2322903/1‑128 (MQ=255)
cTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTaaaaaa                                                                                                                                <  1:2715900/128‑1 (MQ=255)
atttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTaaaaaa                                                                                                                                >  1:6766822/2‑128 (MQ=255)
 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATTCAAATTTTTAAAAaat                                                                                                                               >  1:5643374/1‑128 (MQ=255)
 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                               >  2:7049154/1‑128 (MQ=255)
     aTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaat                                                                                                                           <  1:3050749/128‑1 (MQ=255)
       tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                         <  1:4738461/128‑1 (MQ=255)
       tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                         <  1:5858248/128‑1 (MQ=255)
        gTATTAATTTAATAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTg                                                                                                                        <  1:1724526/128‑1 (MQ=255)
         tATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGa                                                                                                                       <  2:539203/128‑1 (MQ=255)
          aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTGaa                                                                                                                      >  2:2185347/1‑128 (MQ=255)
          aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                      >  2:7756617/1‑128 (MQ=255)
          aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                      <  2:3068803/128‑1 (MQ=255)
            tactttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAAATAAAACTATTATCTTTTTAGATATTTTTTTTATATGCAAATTTTTAAAAAATTAATTAGAACt                                                                                                                    >  2:1922119/1‑128 (MQ=255)
               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                                 >  2:1318615/1‑128 (MQ=255)
               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                                 >  1:6612425/1‑128 (MQ=255)
               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                                 >  2:4148559/1‑128 (MQ=255)
               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                                 >  1:6491750/1‑128 (MQ=255)
               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATATGAACTTGt                                                                                                                 >  1:4915342/1‑128 (MQ=255)
               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGAACATGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTATTAAAAAATTAATTTGAACTAGt                                                                                                                 >  1:13770/1‑128 (MQ=255)
                 tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                               <  2:4303939/128‑1 (MQ=255)
                  actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTAGGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaa                                                                                                              <  1:3196485/128‑1 (MQ=255)
                  actAGTTGATAATATTTAGATTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAAAATTAATTTTAACTTGTaaa                                                                                                              >  1:1996870/1‑128 (MQ=255)
                   ctAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaa                                                                                                             >  2:2066780/1‑128 (MQ=255)
                   ctAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaa                                                                                                             >  1:5463501/1‑128 (MQ=255)
                       ttGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATa                                                                                                         >  2:5233926/1‑128 (MQ=255)
                        tGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAg                                                                                                        >  2:5999058/1‑128 (MQ=255)
                        tGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAg                                                                                                        >  1:6918825/1‑128 (MQ=255)
                             atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttt                                                                                                  >  2:4574387/1‑128 (MQ=255)
                              tatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttt                                                                                                 >  2:7659432/1‑128 (MQ=255)
                               atTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttt                                                                                                >  1:1225121/1‑128 (MQ=255)
                                  tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                             >  1:875858/1‑128 (MQ=255)
                                  tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                             >  1:2461263/1‑128 (MQ=255)
                                    gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttt                                                                                           >  2:146104/1‑128 (MQ=255)
                                    gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttt                                                                                           >  1:2899719/1‑128 (MQ=255)
                                     tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttt                                                                                          >  2:2471749/1‑128 (MQ=255)
                                           tAATTTATCTCCCTTTTTTTAATTTTTAATAAAAAAAAAAAATTATTTTTTTTTATATTTTTTTTTTATTTAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAAAATCtttttttttttttttttt                                                                                    <  1:532596/128‑1 (MQ=255)
                                              tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttaatat                                                                                 >  2:3865253/1‑123 (MQ=255)
                                                 aTCTCACTTGTATTAATTAGTAACAAAAATAAAATATTATATTTTTATTTATTTTTTTTATATTAAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTtataatgg                                                                              <  2:729271/128‑5 (MQ=255)
                                                       cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGCTTTTATTTTTTTTTTTTTTATAAtttaaaa                                                                        >  2:1688471/1‑122 (MQ=255)
                                                       cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttatatttttaata                                                                        >  1:3516625/1‑116 (MQ=255)
                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttttattttaaatat                                                                       >  1:1813200/1‑116 (MQ=255)
                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTAAAatat                                                                      >  2:4976186/1‑128 (MQ=255)
                                                           tATGAATTAGTTATAAACTAAAAAAATTATTATTTTAGATATTTTTTGTTTTATCAAATTTTTAAAAAATTAATTTTTACTTGTTAAAAATA‑‑TGCTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                 <  2:4447437/128‑1 (MQ=255)
                                                           tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAAttaaaaaaaaaa                                                                   >  2:1556637/1‑117 (MQ=255)
                                                            attaaTTATTTATAAAATAAAAATATTTTTTTTTTATTTATTTTTTTTTTATTCAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                 <  1:6353628/125‑1 (MQ=255)
                                                                  tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAATATTGGTAATATAATtttttg                                                             >  1:6208257/1‑126 (MQ=255)
                                                                  tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATTATGTATATATAAAtttttttt                                                           >  1:7874511/1‑124 (MQ=255)
                                                                  tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTAAAATTTAAATATAAATTTTattta                                                          >  1:3844100/1‑124 (MQ=255)
                                                                   attaataaAATAAAACTATTATATTTTTATATATTTTTTTTATATTTAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTa                                                          <  2:3639909/126‑1 (MQ=255)
                                                                   aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATATGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAAAAGTAAATATAAATTTTatg                                                            >  1:1003399/1‑126 (MQ=255)
                                                                    ttaataaaaaaaaaataTTATTTTTTTATATTTTTTTTGTTTATTCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTa                                                          <  1:6113937/118‑1 (MQ=255)
                                                                      aattatataaaaataTTTTTTTTTTTTTTTTTTTTTTTATTTTTTAATTTTTATAAAATTAATTTTAAATTTTTAAAAATTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAtt                                                        <  2:1160671/121‑1 (MQ=255)
                                                                        gAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTATTTATAAAATAAATATAAATATAATTTAtttt                                                      >  2:4729802/1‑128 (MQ=255)
                                                                          aactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTATtttttt                                                   >  2:5506773/1‑128 (MQ=255)
                                                                             taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAAAATAAATTTTATTTATtttttttt                                                 >  2:2882393/1‑126 (MQ=255)
                                                                             taaaaattTTATTTTTTTTTTTTTTTTTTTTATATTGAAATTTTTAAAAAATTATTTTTTAATTTTAAAAAATAGGTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTc                                                <  1:7470962/128‑1 (MQ=255)
                                                                               aaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAATGTAAATATAAATTTTATTTATttttttttata                                              >  2:7535171/1‑123 (MQ=255)
                                                                               aaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTATTTTTTTTTCTa                                              >  2:6332334/1‑128 (MQ=255)
                                                                                aaatattatATTTTTTTTTTTTTTTTTTTTATTCAAATTTTTAAAAAATTAATTTTTAATTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                             <  1:5965355/125‑1 (MQ=255)
                                                                                 aattttatTTTTTTTTATATTTTTTTTATATTTAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                             <  2:2742697/124‑1 (MQ=255)
                                                                                 aaatatataTTTTTATATATTTTTTTTATATTTAAATTTTTAAAAAATTTATTTTTAATTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                             <  1:4405510/123‑1 (MQ=255)
                                                                                  atattatCTTTTTATATATTTTTTTTTTTTGCAAATTTTTAAAAAATTTATTTGAAATTTTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                            <  1:4107845/127‑1 (MQ=255)
                                                                                   tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTAAAATAAAAATTTTATTTATTTTTTTTTATaata                                           >  2:5630112/1‑126 (MQ=255)
                                                                                    attatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATTAAAATTTTAGTTATTTTTTTTTATAATTa                                          >  2:6754720/1‑128 (MQ=255)
                                                                                      tatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATTTAAAAATAAATTTTTGTTTTTTTTTTGTATAATTAta                                        >  1:3355215/1‑127 (MQ=255)
                                                                                       atCTTTTTAGATATTTGTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATTCAAATATAATTTTTAGTTATTTTTTTTTATAAATATCTa                                      >  2:2600179/1‑128 (MQ=255)
                                                                                           tttttttatttttttttttATTTAAATTTTTTATAAATTAAATTTAATTTTTATAAAATTTCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaa                                   <  2:18175/119‑1 (MQ=255)
                                                                                           ttttttatatTTTTTTTTTTTTTAAATTTTTTAAAAAATTATTTTAAATTTTTAAAAATTTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaa                                    <  2:7875335/122‑1 (MQ=255)
                                                                                           ttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATATTTTATATATAATTTTTAGTTATTTTTTTTTATTATTATATaaaaa                                  >  2:3552057/1‑128 (MQ=255)
                                                                                            tttATATTTTTTTTTTTTTTTGAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAAAATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaa                                  <  1:7329495/128‑1 (MQ=255)
                                                                                              tatatatTTTTTGTTTTTGAAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaaaa                                <  2:2322903/125‑1 (MQ=255)
                                                                                              tAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATATTGGATATTTAAATTTTTGGTTTTTTTTTGTCTTAGTTTCTtataaat                                >  1:7061804/1‑127 (MQ=255)
                                                                                               atatatTTTTTTTTTATTCATAATTTTAAAAAATTAAATTGAACTTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                             <  1:3970334/126‑1 (MQ=255)
                                                                                                 ttatTTTTTTTTTATTAAAATTTTTTAAAAATTAATTTTAAATTTTTAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                            <  2:6597676/127‑1 (MQ=255)
                                                                                                 atatTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTATTTTTTTTTAAAATTATATAAAAAAATaa                             >  2:4020969/1‑127 (MQ=255)
                                                                                                  ttttttttttATTTTAAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAATATTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                             <  1:2159381/126‑1 (MQ=255)
                                                                                                   atTTTTTTTTTATTTAAATTTTTAAAAAATTTTTTTTTAATTTTAAAAAATTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGaa                           <  1:4283331/128‑1 (MQ=255)
                                                                                                     ttttttTATATGCAAATTTTTAAAAAATTAATTTGAACTTTTTAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAg                         <  2:7186793/128‑1 (MQ=255)
                                                                                                     tttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGTTATTTTTTTTTATAATTATATAAAAAAATATaaataa                       >  2:105054/1‑125 (MQ=255)
                                                                                                     tttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTATTTTGTTGTATAATTATCTAAAAAAATAGAATGGaa                      >  1:7728586/1‑128 (MQ=255)
                                                                                                       tttttttatTCAAATTTTTAAAAAATTAATTTTAAATTGTAAAAAATAGTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTTTCTAAAAAAATAGAAAgg                        <  2:3768240/122‑1 (MQ=255)
                                                                                                        ttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTATTTTTTTTTATAATTTTATAAAAAAATATAAAgta                       >  2:4856887/1‑126 (MQ=255)
                                                                                                         tttaattttaaaTTTATAAAAAAATAAATTTTAATTTTAAAAAATTTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaa                      <  2:6932038/119‑1 (MQ=255)
                                                                                                         tGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAT‑‑TTTAAATATAAATTTTTTGTATTTTTTTTTATAATTATATAAAAAAATAaaaaataaat                    >  2:6474427/1‑122 (MQ=255)
                                                                                                          ttttttttttaaTTTTTAAAAAATTAATTTTTATTTTTTAAAATAATGTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                     <  1:5137051/118‑1 (MQ=255)
                                                                                                          ttatttttaaaTTTTTAAAAAATTAAATTTTAATTTTAAAAAATTTCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaa                    <  1:7164582/120‑1 (MQ=255)
                                                                                                          gtttatttaaatttttTAAAAAATTATTTTTAATTTTAAAAAATATTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaa                    <  2:7445519/116‑1 (MQ=255)
                                                                                                           tttttttaaaTTTTTAAAAAATTAATTTTTATTTTTAAAAAATTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaaa                   <  2:61443/121‑1 (MQ=255)
                                                                                                             tttttaaaTTTTTAAAAAATTAATTTTAAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat                 <  2:832999/123‑1 (MQ=255)
                                                                                                             tatGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTATTTTTTTGTATAATTATATAAAAAAATAAAAATTAAAAAat                 >  2:512540/1‑128 (MQ=255)
                                                                                                              ttttttatttttTAAAAAATTATTTTTAATTTTTAAAAATTTTTTTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTATGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat                 <  2:3246548/120‑1 (MQ=255)
                                                                                                               tttaaatttttTAAAAATTTATTTTTAATTTTTAAAAATTTTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat                 <  1:1500455/121‑1 (MQ=255)
                                                                                                               tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTTTTTATTTTTTTGTATAATTATATAAAAAAATTAAAAGAAAAAAatat               >  1:7618768/1‑128 (MQ=255)
                                                                                                                 cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTATTTTTTTGTATAATTATATAAAAAAATAGAATGGAAAAAATTTTGt            >  1:370480/1‑128 (MQ=255)
                                                                                                                  aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGATTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTATTTATTTTTTTTTATAATTATATAAAAAAATAAAAAGTAAAAAATAtttt            >  2:5916756/1‑126 (MQ=255)
                                                                                                                    aaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTATAATGTAAAAAAAAATTTTTTGTTTTTTTTTGTAAAATATTAAAAAAAAAAAAAAAGTAAAAAAttttt             >  1:2593137/1‑127 (MQ=255)
                                                                                                                     aTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGAAAATTAAAATTTTAGTTATTTTGTTTTATATTTTTATAATAAAAATTAAATAAAAAATTATTTTTaaa        >  2:5769250/1‑128 (MQ=255)
                                                                                                                            aaaaaaTTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATATTGGAAATATAAATTTTATGTTTTTTTTTGTATAATTAAAAAAAAAAAAACAAAGAAAAAAATATTTTaaaacata   >  1:460420/1‑124 (MQ=255)
                                                                                                                               |                                                                                                                                
CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAAT  >  CP016816/165097‑165349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: