Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 437,599 G→A T33I (ACT→ATT)  JCVSYN2_01985 ← glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816437,5990GA99.1% 10890.6 / NA 3478T33I (ACT→ATT) JCVSYN2_01985glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (1589/1846);  minor base T (9/24);  total (1598/1880)
Fisher's exact test for biased strand distribution p-value = 3.45e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3585 total aligned reads displayed.

AATCAGTTGCTGTATCTTGTGGAATATTATTTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAGTCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTTGTGAAGCAATTATAAAAGACACTAGCATTTTGACACCAACTTTCTTATACTATTT  >  CP016816/437474‑437723
                                                                                                                             |                                                                                                                            
aaTCAGTTGCTGTATCTTGTGGAATATTATTTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATc                                                                                                                            >  1:7371865/1‑128 (MQ=255)
aaTCAGTTGCTGTATCTTGTGGAATATTATTTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATc                                                                                                                            >  1:6918083/1‑128 (MQ=255)
 aTCAGTTGCTGTATCTTGTGGAATAATTTTTTCGCTTAAAAAAATATTTTCTGAGTTATCAAAAATTTCTTTTATATTTTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCt                                                                                                                           <  1:1410271/128‑1 (MQ=255)
  tCAGTTGCTGTATCTTGTGGAATATTATTTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTg                                                                                                                          >  1:2382331/1‑128 (MQ=255)
   cAGGTGCTGTATCTTGTGGAATATTATTTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTgg                                                                                                                         <  1:3248331/128‑1 (MQ=255)
    aGTTGCTGTATCTTGTGGAATATTATTTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTAACTGTATATTTAGAATCACTTATAATAATATTCTgat                                                                                                                        >  2:5556082/1‑126 (MQ=255)
     tttGATGTATCTTTTGGAATATTATTTTAGCTTAAAACTATATTTTCTGAGTTATTAAAAATTTCTTTTATATTTTGTCTTTTAGTTTTATATGTATATTTAGAATCACTTATAATAATAATCTGgtg                                                                                                                       <  1:3731798/127‑1 (MQ=255)
     gTTGCTGTATCTTGTGGAATATTATTTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATATTCTGgtg                                                                                                                       >  1:566692/1‑128 (MQ=255)
     gTTGCTGTATCTTGTGGAATATTATTTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGgtg                                                                                                                       >  1:5155450/1‑128 (MQ=255)
     gTTGCTGTATCTTGTGGAATATTATTTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGgtg                                                                                                                       >  1:4894091/1‑128 (MQ=255)
      ttGCTGTATCTTGTGGAATATTATTTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTCgtgt                                                                                                                      >  1:6658581/1‑128 (MQ=255)
       tGCTGTATCTTGTGGAATATTATTTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATAACTTATAATAATAATCTGGagtg                                                                                                                     >  1:361393/1‑128 (MQ=255)
        gCTGTATCTTGTGGAATATTATTTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGa                                                                                                                    <  1:1993108/128‑1 (MQ=255)
         cTGTATCTTGTGGAATATTATTTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAg                                                                                                                   <  1:2718998/128‑1 (MQ=255)
            tATCTTGTGGAATATTATTTTCGCTTAAAACAATATTTTCTGAGTTATCAAAACTTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGGGAGtta                                                                                                                <  1:6203750/128‑1 (MQ=255)
              tCTTGTGGAATATTATTTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGttatt                                                                                                              >  1:2267838/1‑128 (MQ=255)
              tCTTGTGGAATATTATTTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGttatt                                                                                                              <  2:6738381/128‑1 (MQ=255)
               cTTGTGGAATATTATTTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGttatta                                                                                                             <  1:818279/128‑1 (MQ=255)
                ttGTGGAATATTATTTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGttattat                                                                                                            >  2:1533425/1‑127 (MQ=255)
                ttGTGGAATATTATTTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAg                                                                                                            <  2:6131155/128‑1 (MQ=255)
                ttGTGGAATATTATTTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAg                                                                                                            <  2:4444316/128‑1 (MQ=255)
                ttGTGGAATATTATTTTCGCTTAAAACAATATTTTCTGAGATATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAg                                                                                                            <  2:5106508/128‑1 (MQ=255)
                 tgtgTAATTTTAATTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGt                                                                                                           <  2:35714/128‑1 (MQ=255)
                  gtgGAATATTATTTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGtt                                                                                                          >  2:1701471/1‑128 (MQ=255)
                   tgGAATATTATTTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGttt                                                                                                         >  2:3252479/1‑128 (MQ=255)
                   tgGAATATTATTTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGttt                                                                                                         >  2:750357/1‑128 (MQ=255)
                   tgGAATATTATTTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGttt                                                                                                         >  1:214454/1‑128 (MQ=255)
                       atatTATTTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATg                                                                                                     >  2:4858105/1‑128 (MQ=255)
                        tattatTTTCGCTTAAAACAATATTTTCTGAGTTCTCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGt                                                                                                    <  1:7094023/128‑1 (MQ=255)
                        tattatTTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGATGTGAGTTATTAGTATGATGt                                                                                                    >  1:6050028/1‑128 (MQ=255)
                           aattatagCTTATAAAAAAATTTTCTTATTTATCAAAACTTTCTTTTATATATTGGCTTTTCGTATTCTCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGtttt                                                                                                 <  1:5012248/121‑1 (MQ=255)
                            attTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTta                                                                                                >  2:3444136/1‑128 (MQ=255)
                            attTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTta                                                                                                >  2:7130746/1‑128 (MQ=255)
                            attTTCGCGTAATAGAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTta                                                                                                <  2:3874187/128‑1 (MQ=255)
                              tttCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTTTATATTTAGAATCACTTATAATAATAATCTGTTGTGAGTTATTAGTTTGATGTTTtata                                                                                              >  1:3450715/1‑128 (MQ=255)
                               ttCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAg                                                                                             <  1:6477593/128‑1 (MQ=255)
                                 cGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGaa                                                                                           >  1:4223006/1‑128 (MQ=255)
                                   cTTATAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATc                                                                                         <  2:7358304/128‑1 (MQ=255)
                                   cTTAAAAGAATATTTTCTTAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATc                                                                                         <  2:2291002/128‑1 (MQ=255)
                                   cTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGa                                                                                            >  1:7241458/1‑125 (MQ=255)
                                   cTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATc                                                                                         >  2:6997616/1‑128 (MQ=255)
                                   cTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTATTATATATTGTCTTATAGTAATATCTGTATATTTATAATCACTTATAATAATAATCTGCTGTCAGTTATTAGTTTGATGTATTATAGAATc                                                                                         >  2:4170126/1‑128 (MQ=255)
                                    ttAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCa                                                                                        <  1:1002117/128‑1 (MQ=255)
                                     tAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCaa                                                                                       <  2:5397576/128‑1 (MQ=255)
                                      aaaaCAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAAc                                                                                      >  1:951148/1‑128 (MQ=255)
                                         aCAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTATTAATAAAAATCTGTTGTGAGTTATTAGTTTGATTTTTTATAGAATGAACCgt                                                                                   >  1:1867600/1‑128 (MQ=255)
                                           aaTATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCgttg                                                                                 >  1:5931516/1‑128 (MQ=255)
                                            atatTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCgatgt                                                                                >  1:5268104/1‑128 (MQ=255)
                                             tatTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCgttgtt                                                                               <  2:6876637/128‑1 (MQ=255)
                                             tatTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCgttgtt                                                                               >  2:498893/1‑128 (MQ=255)
                                             tatTTTCTGAGATATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCgttgtt                                                                               <  2:2711268/128‑1 (MQ=255)
                                               ttttCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGtttt                                                                             <  2:5243427/128‑1 (MQ=255)
                                               ttttCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGtttt                                                                             <  2:2114631/128‑1 (MQ=255)
                                                  tCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTTTTAGTTTGATGTTTTATAGAATCATCCGTAGTTTTTaa                                                                          >  1:1141738/1‑128 (MQ=255)
                                                   cTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAat                                                                         <  1:312971/128‑1 (MQ=255)
                                                    tGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAatc                                                                        <  1:4507455/128‑1 (MQ=255)
                                                    tGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAatc                                                                        >  1:1269994/1‑128 (MQ=255)
                                                     gAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAatct                                                                       <  2:6319357/128‑1 (MQ=255)
                                                      aGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAatcta                                                                      >  2:4303170/1‑128 (MQ=255)
                                                       gTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAatctat                                                                     >  1:3032146/1‑128 (MQ=255)
                                                       gTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAatctat                                                                     <  1:256809/128‑1 (MQ=255)
                                                           tCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCa                                                                                        <  1:1561302/105‑1 (MQ=255)
                                                              aaaaTTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGAGGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAAtg                                                              <  2:1384275/128‑1 (MQ=255)
                                                                  tttCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCt                                                          <  2:6602429/128‑1 (MQ=255)
                                                                   ttCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCtt                                                         <  2:5988995/128‑1 (MQ=255)
                                                                     cTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTtgt                                                       >  1:2918984/1‑128 (MQ=255)
                                                                     cTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTAGTTTTTAATCTATCTAAATGTGCttct                                                       >  2:7253919/1‑126 (MQ=255)
                                                                      ttttATTTATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTtgtg                                                      <  2:4029261/128‑1 (MQ=255)
                                                                      ttttATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTtgtg                                                      <  1:750357/128‑1 (MQ=255)
                                                                      ttttATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTtgtg                                                      >  2:5839725/1‑128 (MQ=255)
                                                                           tatatTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTTGTGAAGCa                                                 >  1:4647092/1‑128 (MQ=255)
                                                                           tatatTGTCTTTTAGTAATATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTTGTGAAGCa                                                 <  1:6811691/128‑1 (MQ=255)
                                                                               ttGTCTTTTAGTATTATGTGTATATTTAGAATCACTTATAATATTATTCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTTGTGAAGCAATta                                             <  2:4566455/128‑1 (MQ=255)
                                                                                    ttttAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTta                                                                                                <  2:7656665/72‑1 (MQ=255)
                                                                                      ttAGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTTGTGAAGCAATTATAAAAGa                                      <  1:7813143/128‑1 (MQ=255)
                                                                                        aGTATTATCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTTGTGAAGCAATTATAAAAGaca                                    <  1:5563105/128‑1 (MQ=255)
                                                                                              atCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTTGTGAAGCAATTATAAAAGACACTAGCa                              >  1:3882944/1‑128 (MQ=255)
                                                                                               tCTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTTGTGAAGCAATTATAAAAGACACTAGCAt                             <  2:7890803/128‑1 (MQ=255)
                                                                                                cTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTTGTGAAGCaa                                                <  2:5700525/108‑1 (MQ=255)
                                                                                                cTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTTGTGAAGCAATTATAAAAGACACTAGCAtt                            <  2:7782305/128‑1 (MQ=255)
                                                                                                cTGTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTTGTGAAGCAATTATAAAAGACACTAGCAtt                            >  2:1091370/1‑128 (MQ=255)
                                                                                                  gTATATTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTTGTGAAGCAATTATAAAAGACACTAGCAtttt                          >  1:1680013/1‑128 (MQ=255)
                                                                                                   tataTTTAGTATCACTTATAATTATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAAACGTTGTTTTTAATCTATCTAAATGTGCTTGTGAAGCAATTATAAAAGACACTAGCATTTTg                         <  2:2838314/128‑1 (MQ=255)
                                                                                                   tataTTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGGTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTTGTGAAGCAATTATAAAAGACACTAGCATTTTg                         >  1:5434180/1‑128 (MQ=255)
                                                                                                    atatTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGTTTGTGAAGCAATTATAAAAGACACTAGCATTTTGa                        >  1:4756084/1‑128 (MQ=255)
                                                                                                    atatTTAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTTGTGAAGCAATTATAAAAGACACTAGCATTTTGa                        >  2:2429270/1‑128 (MQ=255)
                                                                                                      atttagctgcACTTATAATAATAATCTGGTGGGAGTTATTAGTGTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTTGTGAAGCAATTATAAAAGACACTAGCATTTTGaca                      <  1:5788240/119‑1 (MQ=255)
                                                                                                       tttAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTTGTGAAGCAATTATAAAAGACACTAGCATTTTGacac                     >  2:856652/1‑128 (MQ=255)
                                                                                                        ttAGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACATTTTTTTTTAATCTATCTAAATGTTCTTTTTAATCAATTATAAAATAAACTAGCATTTTGACAcc                    >  1:4366370/1‑128 (MQ=255)
                                                                                                          aGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATATAATCAACCGTTGTTTTTAATCTATCTAAATGTCCTTGAGAAGCAAATATAAAAGACACTAGCAATATGACACCaa                  >  1:4034432/1‑128 (MQ=255)
                                                                                                          aGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTTGTGAAGCAATTATAAAAGACCCTAGCATTTTGACACCaa                  <  2:2570473/128‑1 (MQ=255)
                                                                                                          aGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTTGTGAAGCAATTATAAAAGACACTATCATTTTGACACCaa                  >  1:7672116/1‑128 (MQ=255)
                                                                                                          aGAATCACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTTGTGAAGCAATTATAAAAGACACTAGCATTTTGACACCaa                  <  2:6440807/128‑1 (MQ=255)
                                                                                                               cACTTATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTTGTGAAGCAATTATAAAAGACACTAGCATTTTGACACCAACTTTc             <  2:4703800/128‑1 (MQ=255)
                                                                                                                  ttATAATAATAATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTTGTGAAGCAATTATAAAAGACACTAGCATTTTGACACCAACTTTCTta          <  2:190254/128‑1 (MQ=255)
                                                                                                                    ataataataATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTTGTGAAGCAATTATAAAAGACACTAGCATTTTGACACCAACTTTCTtata        <  2:2974646/128‑1 (MQ=255)
                                                                                                                     taataataaTCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTTGTGAAGCAATTATAAAAGACACTAGCATTTTGACACCAACt                >  2:3119650/1‑119 (MQ=255)
                                                                                                                     taataataaTCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTTGTGAAGCAATTATAAAAGACACTAGCATTTTGACACCAACTTTCTTATAc       >  1:6332188/1‑128 (MQ=255)
                                                                                                                       ataataATCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTTGTGAAGCAATTATAAAAGACACTAGCATTTTGACACCAACTTTCTTATACTa     >  2:3711285/1‑128 (MQ=255)
                                                                                                                        taataaTCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTTGTGAAGCAATTATAAAAGACACTAGCATTTTGACACCAACTTTCTTATACTAt    <  2:1228306/128‑1 (MQ=255)
                                                                                                                             |                                                                                                                            
AATCAGTTGCTGTATCTTGTGGAATATTATTTTCGCTTAAAACAATATTTTCTGAGTTATCAAAAATTTCTTTTATATATTGTCTTTTAGTATTATCTGTATATTTAGAATCACTTATAATAATAGTCTGGTGTGAGTTATTAGTTTGATGTTTTATAGAATCAACCGTTGTTTTTAATCTATCTAAATGTGCTTGTGAAGCAATTATAAAAGACACTAGCATTTTGACACCAACTTTCTTATACTATTT  >  CP016816/437474‑437723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑