Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 20,023 2 bp→TA intergenic (‑744/‑392) JCVSYN2_00065 ← / → JCVSYN2_00070 hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP01681620,0230AT99.4% 3729.8 / ‑3.5 1249intergenic (‑744/‑393)JCVSYN2_00065/JCVSYN2_00070hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand):  ref base A (4/3);  new base T (682/560);  total (686/563)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP01681620,0240TA99.5% 3765.8 / NA 1248intergenic (‑745/‑392)JCVSYN2_00065/JCVSYN2_00070hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand):  ref base T (4/2);  new base A (681/560);  total (685/563)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1268 total aligned reads displayed.

GCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATAATTTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACAAAAAAAG  >  CP016816/19901‑20145
                                                                                                                          ||                                                                                                                         
 cAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTtat                                                                                                                      <  2:2179089/128‑1 (MQ=255)
 cAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTtat                                                                                                                      <  1:72065/128‑1 (MQ=255)
 cAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCACCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTATTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTtat                                                                                                                      <  2:1539042/128‑1 (MQ=255)
 cAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGAGAGCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTtat                                                                                                                      <  1:755722/128‑1 (MQ=255)
      agagCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTTTATTaa                                                                                                                 >  2:2862164/1‑128 (MQ=255)
      agagCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaa                                                                                                                 <  2:2816862/128‑1 (MQ=255)
       gagCTAGATAAAAGGTAGTATTAGTTGGCGATCCCGCTAGAGTCATTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaa                                                                                                                <  2:3145995/128‑1 (MQ=255)
        agCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaaa                                                                                                               <  2:1170416/128‑1 (MQ=255)
         gCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAatttatatatttatat                                                                                                                    <  1:2451011/122‑1 (MQ=255)
         gCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaaaa                                                                                                              <  1:2618048/128‑1 (MQ=255)
           aaGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAAt                                                                                                            >  2:247950/1‑128 (MQ=255)
            aGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAAtt                                                                                                           <  1:2191982/128‑1 (MQ=255)
              atcaaaGGTAGTATTTGTGTGCGATCCCCCTTGAGTCTTTTACATCTTCGGAAAACAAATACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTa                                                                                                         <  1:1196488/125‑1 (MQ=255)
              aTAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGGCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTATCGATTTTTAATATATATATTTATATTAAAAAATTTa                                                                                                         >  1:3046400/1‑128 (MQ=255)
               tAACAGGTAGTCTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTaa                                                                                                        <  1:862796/128‑1 (MQ=255)
               tAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACCTCATCGGAAAACAAAACCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAAGTTaa                                                                                                        >  1:2250393/1‑128 (MQ=255)
               tAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTaa                                                                                                        <  2:2643888/128‑1 (MQ=255)
               tAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTAGTTTTTCTTTAATTTCTTTTTTTACTGTCTATTTTTAATTTATTTATTTATATTTAAATTTTTat                                                                                                        >  1:1001430/1‑127 (MQ=255)
                aaaaGGTAGTAGTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTaaa                                                                                                       <  2:3184197/128‑1 (MQ=255)
                 aaaGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaat                                                                                                      <  1:357977/128‑1 (MQ=255)
                    ggTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattat                                                                                                   >  1:284339/1‑128 (MQ=255)
                      tagtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataa                                                                                                 >  1:808908/1‑128 (MQ=255)
                        gtaTTTGTTGGTGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatt                                                                                               >  2:1982049/1‑128 (MQ=255)
                        gtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCTGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatt                                                                                               <  2:1436972/128‑1 (MQ=255)
                        gtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAATAAATTTAaattataatt                                                                                               >  1:1684225/1‑128 (MQ=255)
                        gtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatt                                                                                               >  1:1727477/1‑128 (MQ=255)
                         taTTTGTTGGCGAGCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatta                                                                                              >  1:1266782/1‑128 (MQ=255)
                            ttgttgGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATATAAATTATAATTAttt                                                                                           >  1:2504550/1‑128 (MQ=255)
                             tgttgGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATATTTAtttt                                                                                          >  1:2037724/1‑128 (MQ=255)
                             tgttgGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTAtttt                                                                                          >  2:834580/1‑128 (MQ=255)
                             tgttgGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTATATTTTTAATTTATATATTTATATTTAAAAATTTAAATTATAATTAtttt                                                                                          >  1:431865/1‑128 (MQ=255)
                               ttgGCGATCCCCCTAGAGGCTTTTACATCTTAGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTta                                                                                        <  1:626196/128‑1 (MQ=255)
                                 ggCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTtata                                                                                      >  1:409365/1‑128 (MQ=255)
                                  gCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAg                                                                                     <  1:2925982/128‑1 (MQ=255)
                                  gCGATCCCCCTAGAGACTTATACATCTTCTGAAAACAAAAACTATTAATACTTTTATTTATTTTTTTACTTTCTATTTTTAATATATATATTTATATTATAAAATTTAAATAATAATTATTTATATAg                                                                                     >  2:234009/1‑128 (MQ=255)
                                   cGATCCCTCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGc                                                                                    <  1:969036/128‑1 (MQ=255)
                                    gagccaccTAGAGTCTTTTACATGATCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCa                                                                                   <  1:658474/122‑1 (MQ=255)
                                    gATTCCCCTAGAGTCTTTTACATCTTAGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCa                                                                                   <  1:135555/128‑1 (MQ=255)
                                       cccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGt                                                                                <  2:255875/128‑1 (MQ=255)
                                        ccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAATAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                               >  2:664751/1‑128 (MQ=255)
                                        ccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                               <  1:2545188/128‑1 (MQ=255)
                                        ccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                               <  1:2742830/128‑1 (MQ=255)
                                        ccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                               <  1:1535125/128‑1 (MQ=255)
                                        ccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                               >  1:3100501/1‑128 (MQ=255)
                                         cccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGa                                                                              <  2:91120/128‑1 (MQ=255)
                                          ccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGAt                                                                             <  2:3032495/128‑1 (MQ=255)
                                           cTATAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATa                                                                            <  1:90222/128‑1 (MQ=255)
                                            tAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATaa                                                                           >  2:2131408/1‑128 (MQ=255)
                                             agagTCTTTTACATCTTCGGATAAGAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTTAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAAt                                                                          <  1:1934119/128‑1 (MQ=255)
                                             agagTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAAt                                                                          >  2:1843917/1‑128 (MQ=255)
                                             agagTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAAt                                                                          >  1:1305608/1‑128 (MQ=255)
                                                  cTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTaaaa                                                                     >  1:148366/1‑128 (MQ=255)
                                                    tttACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGt                                                                   >  1:487744/1‑128 (MQ=255)
                                                       aCATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAg                                                                <  1:288426/128‑1 (MQ=255)
                                                        cATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAATTTAGt                                                               >  1:1828343/1‑128 (MQ=255)
                                                        cATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGt                                                               >  2:251110/1‑128 (MQ=255)
                                                          tCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATATTTATTTTTATTGCACGTGATATTTAAAAGTTAGTGa                                                             >  1:2402638/1‑128 (MQ=255)
                                                          tCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGa                                                             <  2:724523/128‑1 (MQ=255)
                                                          tCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGa                                                             >  2:1365576/1‑128 (MQ=255)
                                                               ggAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACTTGATAATTAAAAGTTAGTGAACAAg                                                        >  2:2056902/1‑128 (MQ=255)
                                                               ggAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAg                                                        <  2:100382/128‑1 (MQ=255)
                                                                 aaaaCAACAACTATTTTTTCTTTAATTTCATTATTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGaa                                                      <  1:162586/128‑1 (MQ=255)
                                                                       aaaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGt                                                <  1:2803913/128‑1 (MQ=255)
                                                                       aaaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGt                                                <  1:1096025/128‑1 (MQ=255)
                                                                         aaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTTATAATTAAAAGTTATTGAACAAGAAAACAGTGa                                              >  2:623268/1‑128 (MQ=255)
                                                                         aaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGa                                              >  1:143207/1‑128 (MQ=255)
                                                                          aCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTg                                               >  1:1055695/1‑126 (MQ=255)
                                                                          aCTATTTTTTCTTTAATTTCTTATTTTACATTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGAACGTGATAATTAAAATTAAGTGAACAAGAAAACAGTGaa                                             >  1:207890/1‑128 (MQ=255)
                                                                           cTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAg                                            <  1:2285294/128‑1 (MQ=255)
                                                                             aTTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACa                                                          >  2:381299/1‑112 (MQ=255)
                                                                             aTTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCa                                          >  2:2751014/1‑128 (MQ=255)
                                                                              ttttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATATCACGTGATAATTAAATGTTAGTGAAAAAGAAAACatttaatcaa                                         >  2:1732483/1‑119 (MQ=255)
                                                                              ttttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTATTGAACAAGAAAACAGTTAAGCAc                                         >  1:3210830/1‑128 (MQ=255)
                                                                              ttttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGctc                                         >  1:1149243/1‑126 (MQ=255)
                                                                              ttttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAc                                         >  2:1768925/1‑128 (MQ=255)
                                                                              ttttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAc                                         >  1:3159981/1‑128 (MQ=255)
                                                                              ttttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAc                                         >  2:2411006/1‑128 (MQ=255)
                                                                                 tttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAg                                      <  2:293158/128‑1 (MQ=255)
                                                                                   tCTTTAATTTCATTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACATTGAAGCACCAgat                                    >  2:150177/1‑126 (MQ=255)
                                                                                    cTTTAATTTCTATTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAAATATTTTTATAGCACGAGATAATTAAAAGTAAGTGAACAAGAAAACATAGAAGCCCCAGatt                                   >  1:589307/1‑128 (MQ=255)
                                                                                     tttAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTc                                  >  1:54788/1‑128 (MQ=255)
                                                                                          tttCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAAc                             >  2:1191814/1‑128 (MQ=255)
                                                                                            tCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCa                           >  1:235243/1‑128 (MQ=255)
                                                                                             atttttttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACTTGATAATTAAAAGTTAGTTAACAAGAAAACAGTGAATCACCAGTTTCTGAACCaa                          <  2:523979/127‑1 (MQ=255)
                                                                                               ttttttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCaaaa                        <  2:1928161/128‑1 (MQ=255)
                                                                                                 ttttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGc                                           <  2:2274613/107‑1 (MQ=255)
                                                                                                   ttAATTTCTATTTTTAATTTATATATTTATATTAAATAATTTAAATTATAATTATTTTAATAGCACGTGATAATTATAAGTTTGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAAGCAAaagaag                    <  1:2135946/128‑1 (MQ=255)
                                                                                                     aCTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGAc                  >  2:1700446/1‑128 (MQ=255)
                                                                                                      cTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGTACGTGATATTTAAATGTTAGTGAACAAGAAAACAGTGAAGCACCATTTTCTGAACCAAAAGAAGACg                 <  1:2687624/128‑1 (MQ=255)
                                                                                                      cTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACg                 >  2:1062125/1‑128 (MQ=255)
                                                                                                        ttCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaa               <  2:450431/128‑1 (MQ=255)
                                                                                                          cTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaaa             <  2:774050/128‑1 (MQ=255)
                                                                                                            aTTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaaaaa           <  2:1634980/128‑1 (MQ=255)
                                                                                                             tttttAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAAc          >  1:3005309/1‑128 (MQ=255)
                                                                                                             tttttAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAAc          >  2:111305/1‑128 (MQ=255)
                                                                                                               tttAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACTTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAATAAACaa        >  2:1303455/1‑128 (MQ=255)
                                                                                                               tttAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACaa        >  2:125875/1‑128 (MQ=255)
                                                                                                                 tAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACaaaa      <  1:18754/128‑1 (MQ=255)
                                                                                                                    tttATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACaaaaaaa   >  1:197590/1‑128 (MQ=255)
                                                                                                                    tttATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACaaaaaaa   <  1:1768925/128‑1 (MQ=255)
                                                                                                                          ||                                                                                                                         
GCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATAATTTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACAAAAAAAG  >  CP016816/19901‑20145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: