BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	20,023	2 bp→TA	intergenic (‑744/‑392)	JCVSYN2_00065 ← / → JCVSYN2_00070	hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	20,023	0	A	T	99.5%	5002.0 / ‑4.1	1688	intergenic (‑744/‑393)	JCVSYN2_00065/JCVSYN2_00070	hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand): ref base A (6/2); new base T (921/758); total (928/760)
Fisher's exact test for biased strand distribution p-value = 3.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP016816	20,024	0	T	A	99.4%	5071.0 / NA	1688	intergenic (‑745/‑392)	JCVSYN2_00065/JCVSYN2_00070	hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand): ref base T (7/3); new base A (921/757); total (928/760)
Fisher's exact test for biased strand distribution p-value = 5.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1712 total aligned reads displayed.

GCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATAATTTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACAAAAAAAGA  >  CP016816/19901‑20146

||

gCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTta                                                                                                                        <  2:573479/128‑1 (MQ=255)

gCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTta                                                                                                                        >  1:459632/1‑128 (MQ=255)

      agagCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaa                                                                                                                  <  2:3144413/128‑1 (MQ=255)

       gagCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTtat                                                                                                                       >  1:2327495/1‑122 (MQ=255)

       gagCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaa                                                                                                                 <  2:84511/128‑1 (MQ=255)

       gagCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCGATTTTTAATTTATATATTTATATTaaa                                                                                                                 >  1:3459371/1‑128 (MQ=255)

        agCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaaa                                                                                                                >  2:1444442/1‑128 (MQ=255)

        agCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaaa                                                                                                                <  2:512097/128‑1 (MQ=255)

        agCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaaa                                                                                                                >  2:1864650/1‑128 (MQ=255)

            aGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAAtt                                                                                                            >  1:1005374/1‑128 (MQ=255)

            aGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAAtt                                                                                                            >  1:3551410/1‑128 (MQ=255)

             gATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCTGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAAttt                                                                                                           <  1:2835627/128‑1 (MQ=255)

             gATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTGTAATTTCTGTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAAttt                                                                                                           >  1:3866509/1‑128 (MQ=255)

              aTAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTa                                                                                                          <  2:2858088/128‑1 (MQ=255)

               tAAAAGGTAGTATTTGATTGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTaa                                                                                                         <  1:2454177/128‑1 (MQ=255)

                aatatgtagtaTTTGTTGTCGATCAACCTAGAGTCTTTTACATCTTCTGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTaaa                                                                                                        <  1:523629/123‑1 (MQ=255)

                aaaaGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCATTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTaaa                                                                                                        >  2:384916/1‑128 (MQ=255)

                 aaacgaagtagatgttgGCGATCCACCTAGAGTCATTTACATCTTAGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaat                                                                                                       <  1:844170/116‑1 (MQ=255)

                  aaGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaatt                                                                                                      >  2:1594213/1‑128 (MQ=255)

                      tagtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataa                                                                                                  >  1:1880798/1‑128 (MQ=255)

                        gtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATATAaattataatt                                                                                                >  1:919118/1‑128 (MQ=255)

                         taTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatta                                                                                               >  1:3313723/1‑128 (MQ=255)

                          aTTTGTTGGCGTTCACACTAGAGTCTTTTACATCTTCGGAATACAAATACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataattat                                                                                              <  1:1218518/128‑1 (MQ=255)

                          aTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataattat                                                                                              <  2:420137/128‑1 (MQ=255)

                            ttgttgGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTAttt                                                                                            <  1:3017774/128‑1 (MQ=255)

                              gttgGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAAGTATTTTTTCTTTAATTTCTGTTATTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATATTTAttttt                                                                                          >  1:164623/1‑128 (MQ=255)

                              gttgGCGATCCCCATAGAGTCTTTAACATCTTCGGATAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTAttttt                                                                                          <  2:3668298/128‑1 (MQ=255)

                                  gCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTTATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAg                                                                                      <  2:3419560/128‑1 (MQ=255)

                                    gATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCa                                                                                    <  1:2076964/128‑1 (MQ=255)

                                     aTCGCCCTATACTCTATTACATCTTCTGAAAACAAAAAATATTTTATCTTATATTTCTTATTTTACTTTCTAATTTTAATTTAAAAATTTAAATTAAAAAATTTAAATTATAATTATTTTTATAGCAc                                                                                   <  2:248665/128‑1 (MQ=255)

                                     aTCCCCCTCGAGTCTTTTAAATCTTCTGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCAc                                                                                   <  2:262677/128‑1 (MQ=255)

                                      tCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACg                                                                                  >  2:358944/1‑128 (MQ=255)

                                       cccccTAGAGTCTTTTACATCTTCGGAAAACAACAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTAATTTTATAGCACGt                                                                                 >  2:221349/1‑128 (MQ=255)

                                       cccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGt                                                                                 >  2:951690/1‑128 (MQ=255)

                                       cccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTCTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGt                                                                                 >  2:298528/1‑128 (MQ=255)

                                       ccaccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAATAATTTAAATTATAATTATTTTTATAGCACga                                                                                 <  2:3363858/125‑2 (MQ=255)

                                        ccccTAGAGTCTTTTACATCTTCGGAAAACAAAATCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                                <  2:2003862/128‑1 (MQ=255)

                                        ccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                                <  2:1385553/128‑1 (MQ=255)

                                        ccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                                <  1:215055/128‑1 (MQ=255)

                                        ccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                                >  2:134293/1‑128 (MQ=255)

                                        ccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                                <  2:2328518/128‑1 (MQ=255)

                                        ccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTACATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                                <  2:3783296/128‑1 (MQ=255)

                                        ccccTAGAGTCTTATACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                                <  1:718114/128‑1 (MQ=255)

                                          ccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGAt                                                                              <  2:2715827/128‑1 (MQ=255)

                                          ccTAGAGTCTTTTACATATTCGGAAAACTAAATCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTTTTTATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGAt                                                                              <  1:3697381/128‑1 (MQ=255)

                                            tAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaat                                                                                                       <  1:3653072/101‑1 (MQ=255)

                                             agagTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATATAAATTATAATTATTTTTATAGCACGTGATAAt                                                                           >  2:1697308/1‑128 (MQ=255)

                                             agagTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTAAATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAAt                                                                           >  2:1219223/1‑128 (MQ=255)

                                              gagTCGTTTACATCTTATTAAAACAAAAACTATTTTTTCATTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAAtt                                                                          <  1:2748579/128‑1 (MQ=255)

                                               agTCTTTTACATCTTCGGAAGACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTa                                                                         >  2:11003/1‑128 (MQ=255)

                                                  cTTTTACATCTTCTGAATAAAAAATCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTaaaa                                                                      <  2:2801124/128‑1 (MQ=255)

                                                  cTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTAAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTaaaa                                                                      >  1:1155275/1‑128 (MQ=255)

                                                     ttACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGtt                                                                   <  2:3085214/128‑1 (MQ=255)

                                                       aCATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAg                                                                 >  2:3287042/1‑128 (MQ=255)

                                                        cATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTATTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACTTGATAATTAAAAGTTAGt                                                                >  2:2566168/1‑128 (MQ=255)

                                                         aTCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCATATTTTACTTTCTAATTTTAATTTATATATTTATATTAAAAAATTAAAATTAAAATAATTTTTATAGCACTTGATAATTAAAAGTTATTg                                                               >  2:242878/1‑128 (MQ=255)

                                                            ttaggAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAAc                                                            <  1:2946617/125‑1 (MQ=255)

                                                            ttCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTATCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACTAGATAATTAAAAGTTAGTGAAc                                                            >  1:2583373/1‑128 (MQ=255)

                                                              cGGAAAACAATATCTATTTTTTATTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACaa                                                          <  2:811307/128‑1 (MQ=255)

                                                                 aaaaCAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGaa                                                       >  2:1787117/1‑128 (MQ=255)

                                                                  aaaCAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGaaa                                                      >  1:1493564/1‑128 (MQ=255)

                                                                   aaCAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGATCAAGaaaa                                                     >  1:180355/1‑128 (MQ=255)

                                                                       aaaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGt                                                 <  2:3468762/128‑1 (MQ=255)

                                                                       aaaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTCATAATTAAAAGTTAGTGAACAAGGAAACAGt                                                 >  2:1510152/1‑128 (MQ=255)

                                                                       aaaaCTATTTTTTCTTTAAATTCTTTTTTTACTTTCTATTTTTAATATATATATTTATATTAAAAAATTTAAATTATAATTATATTTATAGCACGTCATAATTAAAATTTAGTGAACAAGAAAACAGt                                                 >  2:77362/1‑128 (MQ=255)

                                                                          aCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAATCAGTGaa                                              >  1:199656/1‑128 (MQ=255)

                                                                            tATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGc                                            <  2:2916701/128‑1 (MQ=255)

                                                                            tATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGc                                            <  2:3219367/128‑1 (MQ=255)

                                                                             aTTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACACTGAAGCa                                           >  2:1044558/1‑128 (MQ=255)

                                                                              ttttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAc                                          >  1:1298310/1‑128 (MQ=255)

                                                                              ttttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAc                                          >  2:894282/1‑128 (MQ=255)

                                                                              ttttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAc                                          >  1:641404/1‑128 (MQ=255)

                                                                               tttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAg                                             >  1:2396025/1‑124 (MQ=255)

                                                                               tttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAcc                                         >  1:3384267/1‑128 (MQ=255)

                                                                                  ttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTATAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGt                                      <  1:2205670/128‑1 (MQ=255)

                                                                                   tCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATATCACTTGATAATTAAAAGATATATAACAAGAAAACAGTTAAGCACAAGtt                                     >  1:2508999/1‑128 (MQ=255)

                                                                                       tAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTg                                 >  2:3728530/1‑128 (MQ=255)

                                                                                        aaTTTATTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGa                                <  1:3227653/128‑1 (MQ=255)

                                                                                             cTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAg                                             >  2:6242/1‑110 (MQ=255)

                                                                                             cTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAATCACCAGTGTCTGAACCaa                           >  2:717643/1‑128 (MQ=255)

                                                                                              tttttttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCaaa                          <  1:1720579/128‑1 (MQ=255)

                                                                                              tttttttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCaaa                          >  1:2667458/1‑128 (MQ=255)

                                                                                              tttttttACATTCTAATATTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCaaa                          <  2:1645764/128‑1 (MQ=255)

                                                                                                   ttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACATGAAAACAGTGAAGCACCAGTGTCTGAACCAAaagaag                     >  1:770663/1‑128 (MQ=255)

                                                                                                   ttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAaagaag                     >  2:1293068/1‑128 (MQ=255)

                                                                                                   ttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGAACTTAATAATTAAAATTTATTAAAAAATAAAACATTGATGAACAATTTAATGAACCAAaataag                     >  1:961754/1‑128 (MQ=255)

                                                                                                    tACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACAAGTTTCTGATCCAAAagatga                    >  2:214585/1‑128 (MQ=255)

                                                                                                      cTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACg                  <  1:3791543/128‑1 (MQ=255)

                                                                                                        ttCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaa                <  1:588334/128‑1 (MQ=255)

                                                                                                         tCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCTCCAGTTTCTGAACCAAAAGAAGACGaaa               >  2:1940316/1‑128 (MQ=255)

                                                                                                         tCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaa               >  2:3043575/1‑128 (MQ=255)

                                                                                                         tCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaa               >  1:1407817/1‑128 (MQ=255)

                                                                                                           tATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaaaa             >  2:115929/1‑128 (MQ=255)

                                                                                                            aTTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaaaaa            >  1:1351901/1‑128 (MQ=255)

                                                                                                             tttttAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAAc           >  1:1094394/1‑128 (MQ=255)

                                                                                                                ttAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACaaa        >  2:2062211/1‑128 (MQ=255)

                                                                                                                 tAATTTATATATTTATATTAAAAAATTTAAATTATTATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACaaaa       <  2:3536042/128‑1 (MQ=255)

                                                                                                                 tAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACaaaa       >  1:1560939/1‑128 (MQ=255)

                                                                                                                  aaTTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACaaaaa      >  1:3079010/1‑128 (MQ=255)

                                                                                                                  aaTTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACaaaaa      >  1:3162341/1‑128 (MQ=255)

||

GCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATAATTTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACAAAAAAAGA  >  CP016816/19901‑20146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`