BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	272,629	C→A	A397S (GCT→TCT)	JCVSYN2_01135 ←	dihydroxyacetone kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	272,629	0	C	A	99.5%	4989.5 / NA	1607	A397S (GCT→TCT)	JCVSYN2_01135	dihydroxyacetone kinase
Reads supporting (aligned to +/- strand): ref base C (3/3); major base A (766/827); minor base T (0/8); total (769/838)
Fisher's exact test for biased strand distribution p-value = 8.08e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1607 total aligned reads displayed.

AGATAGATATGAGTAATTTTTTGAAGCTGTTAAAGAAGGATCAAAACTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGCTTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTGAAGGATTCATTTTTGCTCCTGCATTAATT  >  CP016816/272504‑272753

 gatagataTGAGTAATTTTTTGAAGCTGTTAAAGAAGGATCAAAACTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTg                                                                                                                           >  1:1004108/1‑128 (MQ=255)

 gatagataTGAGTAATTTTTTGAAGCTGTTAAAGAAGGATCAAAACTTAAAGCAGCAACCATTACTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTg                                                                                                                           >  2:2924547/1‑128 (MQ=255)

    agataTGAGTAATTTTTTGAAGCTGTTAAAGAAGGATCAAAACTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGttt                                                                                                                        <  1:1518200/128‑1 (MQ=255)

    agataTGAGTAATTTTTTGAAGCTGTTAAAGAAGGATCAAAACTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGttt                                                                                                                        >  1:2823700/1‑128 (MQ=255)

     gataTGAGTAATTTTTTGAAGCTGTTAAAGAAGGATCAAAACTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGtttt                                                                                                                       <  2:1233192/128‑1 (MQ=255)

      atatGAGTAATTTTTTGAAGCTGTTAAAGAAGGATCAAAACTTAAAGCAGCAACCATTCATTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCATATTGTTTTg                                                                                                                      >  2:2840777/1‑128 (MQ=255)

          gAGTAATTTTTTGAAGCTGTTAAAGAAGGATCAAAACTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGt                                                                                                                  <  1:1822491/128‑1 (MQ=255)

              aaTTTTTTGAAGCTGTTAAAGAAGGATCAAAACTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTtaaa                                                                                                              >  2:3699966/1‑128 (MQ=255)

                ttttttGAAGCTGTTAAAGAAGGATCAAAACTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTtatata                                                                                                            >  1:1686778/1‑128 (MQ=255)

                 tttttGAAGCTGTTAAAGAAGGATCAAAACTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTtaaataa                                                                                                           <  1:3260477/128‑1 (MQ=255)

                  ttttGAAGCTGTTAAAGAAGGATCAAAACTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTtaaataaa                                                                                                          >  1:65175/1‑128 (MQ=255)

                   tttGAAGCTGTTAAAGAAGGATCAAAACTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAAc                                                                                                         <  2:1865530/128‑1 (MQ=255)

                       aaGCTGTTAAAGAAGGATCAAAACTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATta                                                                                                     <  2:1407807/128‑1 (MQ=255)

                           tGTTAAAGAAGGATCAAAACTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGt                                                                                                                  <  1:1201854/111‑1 (MQ=255)

                           tGTTAAAGAAGGATCAAAACTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATtactac                                                                                                 <  2:3231024/128‑1 (MQ=255)

                           tGTTAAAGAAGGATCAAAACTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATtactac                                                                                                 >  2:3031154/1‑128 (MQ=255)

                             ttAAAGAAGGATCAAAACTTAAAGCAGCTACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTATTCAGATAGTTTTGCAGTTAAATAAACATTActacta                                                                                               <  1:3181150/128‑1 (MQ=255)

                             ttAAAGAAGGATCAAAACTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTActacta                                                                                               >  1:1630427/1‑128 (MQ=255)

                              tAAAGAATGATCAAAACTTAAAGCAGCAACCATTACTTGTTGAATAGTTAGAGTTTTAATTACATAAAATTTTGATTATATTTCTATTTTATCAGATTGTTATGCAGTTAAATAAACAGTACTTCTAt                                                                                              >  2:260314/1‑128 (MQ=255)

                              tAAAGAATGATCAAAACTTAAAGCAGAAACCATTCATTGTTGAATAGTTTTAGTTTGAATTACATAAATATTTTATTTATTTTATATTTTTTCAGATTTTATTTCAGTTAAATAAACATTACTACTAt                                                                                              >  2:2198642/1‑128 (MQ=255)

                                 agaagGATCAAAACTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTg                                                                                           >  1:1140480/1‑128 (MQ=255)

                                      gATCAAAACTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTTCAGTTAAATAAACATTACTACTATTTGGtaat                                                                                      >  1:1412205/1‑128 (MQ=255)

                                        tCAAAACTTAAAGCAGCATCCATTACTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGtaataa                                                                                    <  1:2168197/128‑1 (MQ=255)

                                        tCAAAACTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGtaataa                                                                                    <  2:1116908/128‑1 (MQ=255)

                                        tCAAAACTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGtaataa                                                                                    <  1:3045965/128‑1 (MQ=255)

                                        tCAAAACTTAAAGCAGCAAAGATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGtaataa                                                                                    <  1:3459774/128‑1 (MQ=255)

                                         cAAAACTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATaaa                                                                                   >  2:1463622/1‑128 (MQ=255)

                                         cAAAACTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATaaa                                                                                   >  2:3574064/1‑128 (MQ=255)

                                         cAAAACTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATaaa                                                                                   <  1:1348043/128‑1 (MQ=255)

                                          aaaaCTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATaaaa                                                                                  <  1:1068451/128‑1 (MQ=255)

                                           aaaCTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATaaaaa                                                                                 >  1:2310310/1‑128 (MQ=255)

                                           aaaCTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTAATTGGTAATaaaaa                                                                                 >  2:923294/1‑128 (MQ=255)

                                           aaaCTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTGTCTATTTTTTCAGATTGTTTTGCAGTTAAATATACATTACTACTATTTGGTAAGaacaa                                                                                 >  1:2415732/1‑128 (MQ=255)

                                           aaaCTTAAAGCAGCAACCATTCCGTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATaaaaa                                                                                 <  2:3779938/128‑1 (MQ=255)

                                             aCTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATa                                                                               >  1:862691/1‑128 (MQ=255)

                                              cTTAAAGCAGCACCCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTTAATAAACATTACTCCTATTTGGTAATATAAATAc                                                                              >  2:2697488/1‑128 (MQ=255)

                                              cTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATAc                                                                              >  2:2083864/1‑128 (MQ=255)

                                                   agcagcAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTAAAATAAACATTACTACTATTTGGTAATAAAAATACTGttt                                                                         <  2:3321800/128‑1 (MQ=255)

                                                    gccgcaACCATTCCTTGTTGAATAGTGATAGGTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACAGtttt                                                                        <  2:1545151/125‑1 (MQ=255)

                                                    gcagcaACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGtttt                                                                        <  1:567069/128‑1 (MQ=255)

                                                    gcagcaACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTATTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGtttt                                                                        <  2:3083316/128‑1 (MQ=255)

                                                     cagcaACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAg                                                                                                                   >  2:213914/1‑84 (MQ=255)

                                                     cagcaACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTa                                                                       >  2:1680288/1‑128 (MQ=255)

                                                      agcaACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAg                                                                      <  1:1884226/128‑1 (MQ=255)

                                                         aaCCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTa                                                                       <  1:2572369/124‑1 (MQ=255)

                                                         aaCCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTATTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGTTAATAAAAATACTGTTTTAGCAt                                                                   >  1:1581916/1‑128 (MQ=255)

                                                          aCCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATc                                                                  <  2:1281051/128‑1 (MQ=255)

                                                           ccATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCa                                                                 <  1:3407034/128‑1 (MQ=255)

                                                             aTTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAAc                                                               >  2:2319460/1‑128 (MQ=255)

                                                               tCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTAGGTAATAAAAATACTGATTTATCATCAACTg                                                             >  1:2735180/1‑128 (MQ=255)

                                                                 cTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTCGGTAATAAAAATACTTTTTTAGCATCAACTGtt                                                           >  2:1722884/1‑128 (MQ=255)

                                                                   ttttgAATAGTTTTAGTTTTAATTACATTAATTTTTTATTTATTTTCTATTTTTTCAGTTTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTc                                                         <  2:1798408/126‑1 (MQ=255)

                                                                   tgttgAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTc                                                         >  2:1171899/1‑128 (MQ=255)

                                                                    gttgAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCt                                                        <  1:3115985/128‑1 (MQ=255)

                                                                      tgAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTAt                                                      >  1:2364617/1‑128 (MQ=255)

                                                                      tgAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTAt                                                      >  1:2253154/1‑128 (MQ=255)

                                                                      tgAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTAt                                                      >  2:854520/1‑128 (MQ=255)

                                                                       gAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTAtt                                                     <  1:3574064/128‑1 (MQ=255)

                                                                         aTAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGc                                                   <  1:3852088/128‑1 (MQ=255)

                                                                            gttttagtttGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCTACTGTTTCTATTGCTTg                                                >  2:344463/1‑128 (MQ=255)

                                                                              tttagtttGAATTAGATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTa                                              <  2:457343/128‑1 (MQ=255)

                                                                              tttagtttGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTa                                              <  1:3732953/128‑1 (MQ=255)

                                                                                tagtttGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGTTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTaag                                            >  1:3345727/1‑128 (MQ=255)

                                                                                tagtttGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTaag                                            >  2:3650997/1‑128 (MQ=255)

                                                                                    ttGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTTCAGTTAAATAAACATTACTACTATTTGTTAATAAAAATACTGTTTTAGCATCAACTATTACTATTTCTTGTAAGAAGt                                        >  1:947747/1‑128 (MQ=255)

                                                                                         ttACATAAATTTATGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCa                                   <  1:7069/128‑1 (MQ=255)

                                                                                           aCATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAATCATTACTACTATTTGGTAATAAAAATACTGTTTTATCTTCAACTTTTTCTATTGCTTGAAAGACGTCtgaagt                                 >  2:2537824/1‑123 (MQ=255)

                                                                                           aCATAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGt                                 <  1:1442446/128‑1 (MQ=255)

                                                                                            cATAAATTTTTGATTTCTTTTCTATTTTTTCAGATTGTTTTGCAGGTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGtt                                <  2:2805211/128‑1 (MQ=255)

                                                                                             aTAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTg                               <  1:2981603/128‑1 (MQ=255)

                                                                                              tAAATTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTGa                              <  1:1261805/128‑1 (MQ=255)

                                                                                                aaTTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCTGTTGAAg                            >  2:244203/1‑128 (MQ=255)

                                                                                                 aTTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTGAAgg                           <  2:2257870/128‑1 (MQ=255)

                                                                                                 aTTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTGAAgg                           >  1:492576/1‑128 (MQ=255)

                                                                                                 aTTTTTGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTGAAgg                           <  2:2981053/128‑1 (MQ=255)

                                                                                                    tttGATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTGAAGGAtt                        <  2:1594575/128‑1 (MQ=255)

                                                                                                    attGATTTATTTTCTATTTTTTCAGAATGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTGAAGGAtt                        <  2:392400/127‑1 (MQ=255)

                                                                                                       gATTTATTTTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTGAAGGATTCAt                     <  1:1754601/128‑1 (MQ=255)

                                                                                                       gATTTATTTTCTATTTTTTCAGATTGTTTTGAAGTTAAATAAACATTACTACTATTTGGTAATATAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTGAAGGATTCAt                     <  2:1043791/128‑1 (MQ=255)

                                                                                                        atttatttTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTGAAGGATTCAtt                    <  1:423917/128‑1 (MQ=255)

                                                                                                           tatttTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTGAAgg                           >  2:1952744/1‑118 (MQ=255)

                                                                                                           tatttTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTGAAGGATTCAttttt                 <  1:3649509/128‑1 (MQ=255)

                                                                                                           taatatCTATTTTTTCAGATTGTTTTGCAGTTAAATAATCATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTGAAGGATTCAttttt                 <  2:2004155/123‑1 (MQ=255)

                                                                                                            atttTCTATTTTTTCAGATTGTTTTGCATTTAAATAAACATTACTAATATTTTGTAATAAAAATACTGTTTTATCATCAACTGTTTCTATTGCTTGTAAGAATTCTTCAGTTGAAGGATTCAttttgt                >  1:2000945/1‑126 (MQ=255)

                                                                                                            atttTCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTGAAGGATTCATTTTTg                <  1:2089304/128‑1 (MQ=255)

                                                                                                             ttttCTATTTTTTCAGAATGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTGAAGGATTCATTTTTGc               <  2:3166361/128‑1 (MQ=255)

                                                                                                              tttCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTGAAGGATTCATTTTTGCt              >  1:2659302/1‑128 (MQ=255)

                                                                                                                tCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTGAAGGATTCATTTTTGCTcc            >  1:78184/1‑128 (MQ=255)

                                                                                                                tCTATTTTTTCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTGAAGGATTCATTTTTGCTcc            >  1:2895183/1‑128 (MQ=255)

                                                                                                                  tatgtattCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTACTAAAAATACTGGTTTAGCAGCAACTGTTTCTATTGCTTGTACGAAGGCTTCAGTTGAAGGATTCATTTTTGCTCCTg          <  2:351481/122‑1 (MQ=255)

                                                                                                                    ttttatAAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTGAAGGATTCATTTTTGCTCCTGCa        <  2:631429/128‑1 (MQ=255)

                                                                                                                      ttttCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTGAAGGATTCATTTTTGCTCCTGCAtt      <  1:2464454/128‑1 (MQ=255)

                                                                                                                      ttttCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTCGAAAAAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGAAAGAATTCTTCAGTTGAAGGATTCATTTTTGCTCCTGCAtt      >  2:679925/1‑128 (MQ=255)

                                                                                                                       tttCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTGAAGGATTCATTTTTGCTCCTGCATTa     >  2:771747/1‑128 (MQ=255)

                                                                                                                       tttCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGGTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTGAAGGATTCATTTTTGCTCCTGCATTa     <  2:2753107/128‑1 (MQ=255)

                                                                                                                        ttCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTGAAGGATTCATTTTTGCTCCTGCATTaa    <  1:3793201/128‑1 (MQ=255)

                                                                                                                        ttCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTGAAGGATTCATTTTTGCTCCTGCATTaa    >  1:3521075/1‑128 (MQ=255)

                                                                                                                         tCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTGAAGGATTCATTTTTGCTCCTGCATTAAt   >  2:3387615/1‑128 (MQ=255)

                                                                                                                         tCAGATTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTGAAGGATTCATTTTTGCTCCTGCATTAAt   <  2:1362430/128‑1 (MQ=255)

                                                                                                                         tCAGATTGTATTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTGAAGGATTCATTTTTGCTCCTGCATTAAt   <  2:541639/128‑1 (MQ=255)

AGATAGATATGAGTAATTTTTTGAAGCTGTTAAAGAAGGATCAAAACTTAAAGCAGCAACCATTCCTTGTTGAATAGTTTTAGTTTGAATTACATAAATTTTTGATTTATTTTCTATTTTTTCAGCTTGTTTTGCAGTTAAATAAACATTACTACTATTTGGTAATAAAAATACTGTTTTAGCATCAACTGTTTCTATTGCTTGTAAGAAGTCTTCAGTTGAAGGATTCATTTTTGCTCCTGCATTAATT  >  CP016816/272504‑272753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`