Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816202,4850T.74.5% 335.0 / inf 287intergenic (+263/‑412)JCVSYN2_00840/JCVSYN2_00845hypothetical protein/lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (17/46);  new base . (73/111);  total (101/186)
Fisher's exact test for biased strand distribution p-value = 9.47e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.67e-01

Only 100 of 316 total aligned reads displayed.

AAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTTTTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATAT  >  CP016816/202358‑202603
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  gTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGATTATCTTCACAAAAAAAAAAAAAACAATCCCAtta                                                                                                                          >  1:3122846/1‑127 (MQ=255)
   ttAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATAACCttaa                                                                                                                         >  2:3531241/1‑126 (MQ=255)
     attgtAAATAAAATAGTTAATTTTAATTAAAATAACATAAAATTAATAATTCTTATAATTTATAAACTCGTTATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCttaaaa                                                                                                                       <  1:102321/126‑5 (MQ=255)
     aGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCttaaaa                                                                                                                       >  2:3265749/1‑124 (MQ=255)
      gtgtAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCttaaaaa                                                                                                                      >  2:3419984/1‑123 (MQ=255)
      gtgtAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCACtaaaaaa                                                                                                                      >  1:3852786/1‑122 (MQ=255)
         tAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAATCAATCCACttaaaaatta                                                                                                                   >  2:1465842/1‑120 (MQ=255)
         tAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCttaaaaataa                                                                                                                   >  1:3141971/1‑120 (MQ=255)
           aataatAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCTTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAAAATCCCCtaaaaaattaaa                                                                                                                 >  2:2945680/1‑117 (MQ=255)
                taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑‑TAAAAAATtaaaaaaaa                                                                                                        >  1:3263757/1‑128 (MQ=255)
                taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCC‑‑ATTAAAAATTaaaaaaaa                                                                                                         >  1:3165260/1‑128 (MQ=255)
                 agagTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCTTCGTGATCTAATATTATATTTGTATATCTTCACAAATAAAAAAAAAACAAACCAC‑‑‑AAAAAAATaaaaaaaaaa                                                                                                      >  2:2223241/1‑128 (MQ=255)
                 agagTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTATTTATTTGTTTATCATCACAAATAAAAAAAAAACAAACACATAaaaaaaaaaaaaaaaa                                                                                                          >  2:821697/1‑128 (MQ=255)
                     ttAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGCTATATTTTTTTATTATTTTATATTAAGAAATAAAAATAAAACAATAC‑‑‑AATAAAaaataaaaaaaataaaaa                                                                                                  >  2:2110148/1‑128 (MQ=255)
                     ttAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaa                                                                                                   >  2:376524/1‑128 (MQ=255)
                      tAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCTTGATCTATTTTTTTATTTTTTTATCTTCACAAATAAAAAAAAAAAAAACACCTT‑‑‑AAAAATaaaaaaaaaaaaaaa                                                                                                 >  2:1061279/1‑128 (MQ=255)
                      tAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAACCC‑‑AAAAAAAAATaaaaaaaaaaaaaa                                                                                                  >  2:1547589/1‑128 (MQ=255)
                       tattttaaataaAATAACATAAAATTAAAAATTATTTAAATTTATATACACGTTTTATATTTTTTTATTTTTTTATATTAACAAATAAAAAAAAAACAATACCC‑‑TTAAAAATTaaaaaaaaaaaaaaa                                                                                                 <  1:3544420/124‑1 (MQ=255)
                       aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTTAAAAATTATaaaaaaaaaaaaa                                                                                                   >  2:451919/1‑128 (MQ=255)
                       aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaa                                                                                                 >  2:3193363/1‑128 (MQ=255)
                       aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaa                                                                                                 >  2:3619315/1‑128 (MQ=255)
                       aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTATTATATTTCTTTATCTTCACAAATAAAAAAAAAACAATCACC‑‑‑TAAAAAATtaaaaaaaaaaaaaaa                                                                                                >  2:203155/1‑128 (MQ=255)
                        atgTTTATTAAAATAAAATAAAATTAATAATTTTTATAATTTTTTTACTTGTTTTTTATTTTTTTTTTTTTTTATTTTAAAAAATAAAAAAAAAAAAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaa                                                                                                <  2:2997617/126‑1 (MQ=255)
                        aGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAAAAACCC‑‑‑ATAAAAAATaaaaaaaaaaaaaaaaa                                                                                               >  2:1952218/1‑128 (MQ=255)
                        aGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATATTTTTATTTTTTTATAATCACAAATAAAAAAAAAAAAA‑‑ACAAATTAAAAATTaaaaaaaaaaaaaaaa                                                                                                >  2:2572302/1‑128 (MQ=255)
                         ttttaataatAATACAATAAAATTAATATATATAATAATTTTTATCATCGGTTTTTTTTTTTTTTTTTTTATATATTAAAAAATAAAAAAAAAACAATCCCC‑‑TTAAAAAATaaaaaaaaaaaaaaaaa                                                                                               <  1:3250595/126‑1 (MQ=255)
                         ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaa                                                                                               >  2:3179824/1‑128 (MQ=255)
                         ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATACC‑‑‑ATAAAAAATaaaaaaaaaaaaaaaaaa                                                                                              >  2:3879196/1‑128 (MQ=255)
                          ttttattaaAAAAAAATAAAATTAAAAATTTTTTTAATTTTTATCCTTTTTTTATTTTTTTTTATTTTTTTATTTTAAAAAATAAAAAAAAAAAAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaa                                                                                              <  2:1158472/124‑1 (MQ=255)
                           ttaattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATACAC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaa                                                                                             >  1:2930303/1‑128 (MQ=255)
                            taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATATTTTTATTTGTATATCTTCAAAAATAAAAAAAAAACAATCCAATTAaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                              >  2:2441219/1‑128 (MQ=255)
                            taattaaAATACCATAAACTTAATAATTCTTATAATGAAGATCCTCGTGATATATTTTTTTATTTGTTTATCTTAACAAATAAAAAAAAAAACAAACCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaata                                                                                             >  2:3018495/1‑126 (MQ=255)
                             aattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCACATTAAAAATaaaaaaaaaaaaaaaaaaaaaa                                                                                             >  1:982653/1‑128 (MQ=255)
                             aattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAAAATCACC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaa                                                                                           >  1:3508557/1‑128 (MQ=255)
                             aattaaAATACCATAAACTTAATAATTCTTATAATGAAGATCCTCGTGATCTATTTTTTTATTTGTTTATATTAACAAAAAAAAAAAAAAAAATACC‑‑ATAAAAAAATaaaaaaaaaaaaaaaaaaaaa                                                                                           >  2:749731/1‑128 (MQ=255)
                             aaataaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTTTTTATCTTCACAAATAAAAAAAAAAAAAACAAC‑‑‑AAAAAAATaaaaaaaaaaaaaaaaaaaaaa                                                                                          >  2:128951/4‑127 (MQ=255)
                              attaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTTATCTATTTTTTTATTTTTTTATCTTCACAAATAAAAAAAAAACAA‑‑ACCAATAAAAAAATaaaaaaaaaaaaaaaaaaaaaa                                                                                          >  2:1761423/1‑127 (MQ=255)
                              attaaAATACCATAAACTTAAGAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAACCCC‑‑TTAAAATATaaaaaaaaaaaaaaaaaaaaaa                                                                                          >  2:891520/1‑127 (MQ=255)
                                 aaatTAACATAAACATAATATTTATTATAAAGTTTTACTTCTTGATCTATTTTTTTATTTTTTTATCTTAACAAATAAAAAAAAAACAATCCCCTT‑‑‑AAAATTTAAAAAAAAAAAAAAAAAAAACTaaa                                                                                      <  1:2223241/128‑1 (MQ=255)
                                  aaataaaaaaaaaTTAAAAATTCTTATAATTTACTTCCTAGTTATCTAATTTTTTAATTTTTTATATTAAAAAAAAAAAAAAAAAAAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTaaa                                                                                      <  1:3375530/119‑1 (MQ=255)
                                   aaTACCATAAACTTAATAATTCTTATAATTTAGATCCTCGTGATCTATTTTATTATTTTTTTATCTTAACAAATAAAAAAAAAAAAAAACAC‑‑TTAAAAATTAaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                     >  2:663052/1‑128 (MQ=255)
                                   aaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTAaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                     >  2:3444236/1‑128 (MQ=255)
                                    aaaataTAAAATTAATAATTTTTATAATTTATTTACTGTTTTTTTATTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAAAAAACCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTaaaaa                                                                                    <  1:3080428/123‑1 (MQ=255)
                                        cATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAAAATCCC‑‑ATTAAAAATTAAAAAAAAAAAAAAAAAAAAAATAaaaaaaaa                                                                                >  2:230255/1‑128 (MQ=255)
                                        cATAAAATTAATAATTATTATAATGTTTATACTCGTTATATATTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAAAAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAaaacaaa                                                                                <  1:3187775/128‑1 (MQ=255)
                                           aaaaataaaaaTTATTATAATTTATATACACTTTATTTATTTTTTTATTTTTTTATATTCACAAAAAAAAAAAAAAAAAACCCCTT‑‑‑AAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                          <  1:1276140/123‑1 (MQ=255)
                                              attAATAATTTAAATAATTTATAAACTCTGGATAAATTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAAAAATACCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAActct                                                                           <  1:3743939/127‑1 (MQ=255)
                                                  ataatTCTTATAATGTAGAAACTAGTTATATATTTTTTTATTTTTTTATCTTAACAAATAAAAAAAAAAAAATACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTa                                                                   <  1:1378216/128‑1 (MQ=255)
                                                     aatCTTATAATGTAGATCCTCGTGATCTATTATTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAA‑‑ACCAAAAAAAAATTAAAAAAAAATAAAAAAAAAAAAAAAAAAACAAACTAAAtttataa                                                                   >  2:919304/3‑124 (MQ=255)
                                                       ttataaaaTTTATATACTAGTTATCTATTTTTTTAATTTTTTAACTTAAAAAAAAAAAAAAAAAAAAACCCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTaaa                                                                 <  1:1071951/122‑1 (MQ=255)
                                                                  aGATCCTAGTTTTATATTTTTTTTTTTTTTTTTATTAAAAAAAAAAAAAAAAAAAAACCCC‑‑TTAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtattat                                                    <  1:3619315/128‑1 (MQ=255)
                                                                     tctacttgtgatatttttttattttttTATATTAAAAAAAAAAAAAAAAAAAATACCC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAcc                                                 <  1:892477/117‑1 (MQ=255)
                                                                     tacTCGTTATCTATTTTTTTATATGTTTATATTAACAAATAAAAAAAAAACAATACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcac                                               <  1:579526/126‑1 (MQ=255)
                                                                      aaatatttattATTTTTTTAATTTTTTAAAAAAAAAAAAAAAAAAAAAAAAATACC‑‑ATAAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                <  1:1590884/118‑1 (MQ=255)
                                                                       tattgttttttttttttttttttttttttttAAAAAAAAAAAAAAAAAAAAAACCCTTT‑‑AAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcac                                               <  2:3368480/117‑1 (MQ=255)
                                                                        ttgttttattttttttttttttttTATTTTAAAAAAAAAAAAAAAAAAAATAACATTAAAAAATAAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAcc                                                 <  1:212113/118‑1 (MQ=255)
                                                                         tttatatattttttttATTTTTTATATTAACAAAAAAAAAAAAAAAAATCACCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                           <  1:3572385/119‑1 (MQ=255)
                                                                           ttaTCTATTTTTTATATTTTTTATTTAAAAAAAAAAAAAAAAAAAAAACACC‑‑ATAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                           <  1:866064/126‑1 (MQ=255)
                                                                            tatatatttttttattttttTATTATTAAAAATAAAAAAAAAACAATCCCCTTAATAATTAAAAAAAAAAAAAAAAAAAAAACTAAACACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                           <  2:371498/124‑1 (MQ=255)
                                                                            tatatatttttttattttttTATCATTAAAAATAAAAAAAAAACAATTCCC‑‑TTAAAATTTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttt                                          <  1:3442654/124‑1 (MQ=255)
                                                                              tattttttttttttTGTTTTTATTCACAAATAAAAAAAAAAAAATACCCTTAAAAATAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttta                                         <  1:2970623/124‑1 (MQ=255)
                                                                              tatATTTTTTTTTTTTTTTATATTAAAAAATAAAAAAAAAAAAAAACACTTAAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttta                                         <  1:2956551/126‑1 (MQ=255)
                                                                              aaaaTTTTTTTATTTGTATATATTAAAAAAAAAAAAAAAAACAAAACCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttt                                          <  1:1526262/125‑1 (MQ=255)
                                                                                tttttttttatttttttaTATTAAAAAATAAAAAAAAAAAAATAACCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                     <  2:3395194/126‑1 (MQ=255)
                                                                                 aTTTTTTTATTTGTTTAAATTAAAAAAAAAAAAAAAAAAAATACCC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                     <  1:3688227/128‑1 (MQ=255)
                                                                                  tttttttatttttttaTCTTAACAAATAAAAAAAAAACAATCCCCAT‑‑‑AAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaa                                  <  1:3228072/128‑1 (MQ=255)
                                                                                  tttttttATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACAAAAAACAAACCACAAATAATTAAAATACCCTTTTATACCCAATTATAttttt                                    >  2:278313/1‑128 (MQ=255)
                                                                                  tttttttAATTTTTTATCTTCACAAATAAAAAAAAAACAATCCC‑‑ATTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                    <  1:2866647/128‑1 (MQ=255)
                                                                                  attttttATTTGTTTATATTCAAAAATAAAAAAAAAAAATCACC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                   <  1:1196883/127‑1 (MQ=255)
                                                                                  attttttATTTGTTTATATACAAAAAAAAAAAAAAAAAAAACCCC‑‑‑TAAAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                   <  2:3641617/127‑1 (MQ=255)
                                                                                   ttttttattttttaTATTAAAAAAAAAAAAAAAAAAAAAACCC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaa                                  <  1:3090145/128‑1 (MQ=255)
                                                                                    tttttatttttttattTTAAAAAAAAAAAAAAAAATAAACCCATTTAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                   <  1:2403657/128‑1 (MQ=255)
                                                                                    tttttATTTTTTTATCTTAAAAAATAAAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAGAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaa                                  <  1:2299585/128‑1 (MQ=255)
                                                                                    tttttATTTTTTTTATTAAAAAAAAAAAAAAAAAAAAAACCCTTAAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaa                                  <  1:476096/128‑1 (MQ=255)
                                                                                    atattatatttattATATTAAAAAAAAAAAAAAAAATAAACCCC‑‑TAAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTAATTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                   <  1:2634638/116‑1 (MQ=255)
                                                                                     ttttATTTTTTTATCTTAAAAAAAAAAAAAAAAAAAAAACCCTAAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaa                                  <  1:2160335/128‑1 (MQ=255)
                                                                                      tttATTTTTTTATAATAAAAAATAAAAAAAAAAAAT‑‑ACTATTAAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATa                              <  1:1818579/128‑1 (MQ=255)
                                                                                       ttATTTTTTTATCTTAACAAAAAAAAAAAAAAAAATACCC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAAt                               <  1:3534162/128‑1 (MQ=255)
                                                                                       ttATTTTTTTATATTTAAAAAAAAAAAAAAAAAAAAACCCATAAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaaaa                                <  1:1329813/128‑1 (MQ=255)
                                                                                          tttttttTATTTCAAAAATAAAAAAAAAAAAATACCATTAAAAATTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATa                              <  1:2090225/127‑1 (MQ=255)
                                                                                           ttttttTATTTAACAAAAAAAAAAAAAAAAAACACCATAAAAATTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATaa                             <  1:775029/128‑1 (MQ=255)
                                                                                              ttttttattaataaaaaaaaaaaaaaaaATACCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGata                         <  1:2014271/116‑1 (MQ=255)
                                                                                              tttaatattaaaaaaaaaaaaaaaaaCAATACC‑‑ATTAAAAATTTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGata                         <  1:190490/116‑1 (MQ=255)
                                                                                               ttttatttCAAAAAAAAAAAAAAAAAAAAACCCTTAAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGata                         <  2:3123633/122‑1 (MQ=255)
                                                                                               tttaatatataaaaTAAAAAAAAAAAAAAACCATTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGata                         <  2:3330518/117‑1 (MQ=255)
                                                                                               tttATCTTCACAAATAAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAA‑‑AAAACCAAAATATATTTATTAAAATTACATATTAAACCAAATATAATTTTTAAAAAAAGATAttta                     >  2:3150396/1‑126 (MQ=255)
                                                                                                ttaatataaaaaaTAAAAAAAAAAAAATAACC‑‑ATAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATAtt                       <  1:3826262/118‑1 (MQ=255)
                                                                                                   ttaattaaaaaaaaaaaaaaaaaaaACCC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                   <  1:1350260/121‑1 (MQ=255)
                                                                                                   tcataaaaaaTAAAAAAAAAAAAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGAc                    <  2:3072440/121‑1 (MQ=255)
                                                                                                    tataaaaaataaaaaaaaaaaaaATCCCC‑‑‑TAAAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                   <  2:3793067/117‑1 (MQ=255)
                                                                                                    attaacaAATAAAAAAAAAAAAAAAACC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                   <  1:3331400/124‑1 (MQ=255)
                                                                                                     ttaaaaaaaaaaaaaaaaaaaATAACCATAAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                   <  2:3552557/123‑1 (MQ=255)
                                                                                                     taaaaaaaaaaaaaaaaaaaaaaACCC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACata                 <  1:1989921/123‑1 (MQ=255)
                                                                                                     aataaaaaaaaaaaaaaaaaaaaaaCCATATAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                   <  1:2751028/123‑1 (MQ=255)
                                                                                                       aaaaaaTAAAAAAAAAACAATACCC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACataat               <  1:1136955/125‑1 (MQ=255)
                                                                                                        acaAATAAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACAAAAACCAAAAAAAATTTATAAAAATACCAATATTTACCACTTTTTTTTTTTAAAAAAATAtttttaataaaaa              >  2:292827/1‑119 (MQ=255)
                                                                                                         aaaaaaaaaaaaaaaCAAAACC‑‑ATTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACataata              <  1:519753/127‑1 (MQ=255)
                                                                                                                  aaaaaaaCAATAACCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAatatatatt        <  1:698782/128‑2 (MQ=255)
                                                                                                                     aaaaaaaaacccATTAAAAATAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTACCTATTATACCAAATTTTATTTTTAAAATAAGATATTGACATAATATATATCTaa     <  1:2521671/119‑1 (MQ=255)
                                                                                                                     aaaaCAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAACTATTTATTAAAATTACCTTTTTTAAAACATTTTATTTTTAAAATAAAATATTCACATAatatataaataaaaa  >  1:3049103/1‑120 (MQ=255)
                                                                                                                                 |                                                                                                                          
AAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTTTTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATAT  >  CP016816/202358‑202603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: